Tilletiopsis washingtonensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Exobasidiomycetes; Entylomatales; Entylomatales incertae sedis; Tilletiopsis

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316Z1M4|A0A316Z1M4_9BASI E3 ubiquitin ligase complex SCF subunit OS=Tilletiopsis washingtonensis OX=58919 GN=FA09DRAFT_312931 PE=3 SV=1
MM1 pKa = 7.11LHH3 pKa = 6.55LWHH6 pKa = 6.43VVSAFALLLLQARR19 pKa = 11.84ALEE22 pKa = 4.32FAFTTFSCNEE32 pKa = 3.37VRR34 pKa = 11.84FTYY37 pKa = 10.95ALDD40 pKa = 3.35QNTWEE45 pKa = 4.14RR46 pKa = 11.84QANVLLINYY55 pKa = 9.21RR56 pKa = 11.84DD57 pKa = 3.64EE58 pKa = 4.43TIAAVDD64 pKa = 3.52INSPSGTGSFDD75 pKa = 3.15PTVITEE81 pKa = 4.1AARR84 pKa = 11.84YY85 pKa = 9.39DD86 pKa = 3.52FTVALGSDD94 pKa = 3.47ACDD97 pKa = 3.22VFATDD102 pKa = 4.75GACFEE107 pKa = 4.61AGRR110 pKa = 11.84NQLYY114 pKa = 10.75ALTISQQATCDD125 pKa = 3.67GEE127 pKa = 4.64GTGDD131 pKa = 4.62ADD133 pKa = 3.66ATSNLPADD141 pKa = 4.67GAQSSEE147 pKa = 3.97ADD149 pKa = 3.49EE150 pKa = 5.38ADD152 pKa = 3.6NQALNSAVVPDD163 pKa = 5.77DD164 pKa = 3.46IAHH167 pKa = 6.21TAGAVTVSVGSAEE180 pKa = 4.11ASSTGSGAQTATPTSSAAPTTATLIPLPLSSTAPAANAASARR222 pKa = 11.84AGWSGAAVLAALVLAAAVV240 pKa = 3.46

Molecular weight: 24.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316Z7X5|A0A316Z7X5_9BASI Uncharacterized protein OS=Tilletiopsis washingtonensis OX=58919 GN=FA09DRAFT_30911 PE=4 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84TSLTRR9 pKa = 11.84LLPQGTLSRR18 pKa = 11.84APHH21 pKa = 4.91RR22 pKa = 11.84TPRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 5.05PTLLPRR34 pKa = 11.84RR35 pKa = 11.84STRR38 pKa = 11.84SRR40 pKa = 11.84TLPTPSCVPPRR51 pKa = 11.84RR52 pKa = 11.84PRR54 pKa = 11.84AASPPPPRR62 pKa = 11.84LPRR65 pKa = 11.84TASRR69 pKa = 11.84RR70 pKa = 11.84LRR72 pKa = 11.84RR73 pKa = 11.84QSSRR77 pKa = 11.84ARR79 pKa = 11.84ATSHH83 pKa = 6.12RR84 pKa = 11.84RR85 pKa = 11.84QRR87 pKa = 11.84TASSARR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84ASTPRR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84VTTSRR109 pKa = 11.84ASSARR114 pKa = 11.84SRR116 pKa = 11.84WHH118 pKa = 6.21GRR120 pKa = 11.84SPMPVAMCKK129 pKa = 9.6PAA131 pKa = 4.87

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7005

2

7007

3617120

49

5324

516.2

55.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.01 ± 0.049

1.152 ± 0.014

5.126 ± 0.02

5.888 ± 0.026

2.843 ± 0.018

7.537 ± 0.025

2.386 ± 0.012

3.04 ± 0.02

3.36 ± 0.025

9.263 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.854 ± 0.009

2.139 ± 0.016

6.834 ± 0.035

3.886 ± 0.024

7.766 ± 0.03

8.973 ± 0.043

5.024 ± 0.019

5.767 ± 0.021

1.235 ± 0.009

1.919 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski