Streptococcus phage Javan274

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AXF0|A0A4D6AXF0_9CAUD Portal protein OS=Streptococcus phage Javan274 OX=2548089 GN=Javan274_0024 PE=4 SV=1
MM1 pKa = 6.58ICYY4 pKa = 9.67YY5 pKa = 11.18AFVFDD10 pKa = 5.47LDD12 pKa = 3.33ISLVLEE18 pKa = 4.67FSVHH22 pKa = 6.73DD23 pKa = 4.22LDD25 pKa = 6.18EE26 pKa = 4.47IALCKK31 pKa = 10.18LAIEE35 pKa = 4.13NWKK38 pKa = 10.54KK39 pKa = 11.08SEE41 pKa = 3.86

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AY25|A0A4D6AY25_9CAUD Cytosine-specific methyltransferase OS=Streptococcus phage Javan274 OX=2548089 GN=Javan274_0038 PE=3 SV=1
MM1 pKa = 7.55LNLVEE6 pKa = 5.16PIRR9 pKa = 11.84DD10 pKa = 3.49KK11 pKa = 11.73DD12 pKa = 4.28DD13 pKa = 3.22IQAMKK18 pKa = 10.44DD19 pKa = 3.7YY20 pKa = 10.24LLHH23 pKa = 7.07DD24 pKa = 3.71SAMSYY29 pKa = 8.24EE30 pKa = 3.93RR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 9.27RR34 pKa = 11.84NYY36 pKa = 9.95MIFLTGINSGLRR48 pKa = 11.84VGDD51 pKa = 4.22IIQLKK56 pKa = 9.77VKK58 pKa = 10.2DD59 pKa = 3.73VQGWHH64 pKa = 6.18IRR66 pKa = 11.84VKK68 pKa = 8.57EE69 pKa = 3.95QKK71 pKa = 8.66TGKK74 pKa = 8.01TRR76 pKa = 11.84TFRR79 pKa = 11.84MTKK82 pKa = 8.97PLKK85 pKa = 9.6NAMRR89 pKa = 11.84EE90 pKa = 4.1YY91 pKa = 11.23VKK93 pKa = 10.61DD94 pKa = 3.75KK95 pKa = 10.4PLHH98 pKa = 6.05HH99 pKa = 6.95YY100 pKa = 9.67IFKK103 pKa = 10.73SRR105 pKa = 11.84EE106 pKa = 3.88GHH108 pKa = 5.88NKK110 pKa = 9.82PITSGQVWNIIKK122 pKa = 10.07EE123 pKa = 3.99AADD126 pKa = 3.83FLGIDD131 pKa = 3.86NIGTHH136 pKa = 5.92SMRR139 pKa = 11.84KK140 pKa = 6.95TYY142 pKa = 10.23GYY144 pKa = 10.05HH145 pKa = 6.79HH146 pKa = 6.86YY147 pKa = 10.75KK148 pKa = 9.87QFKK151 pKa = 10.04DD152 pKa = 3.48VAALMEE158 pKa = 4.61TFNHH162 pKa = 6.72ASPMITLRR170 pKa = 11.84YY171 pKa = 9.53IGIKK175 pKa = 9.36QDD177 pKa = 3.57EE178 pKa = 4.62LDD180 pKa = 3.72DD181 pKa = 4.09FQRR184 pKa = 11.84KK185 pKa = 7.75FALL188 pKa = 3.85

Molecular weight: 22.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10965

41

1135

210.9

23.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.498

0.565 ± 0.108

6.183 ± 0.356

7.469 ± 0.349

4.332 ± 0.213

6.156 ± 0.339

1.596 ± 0.199

7.925 ± 0.33

8.39 ± 0.389

8.098 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.137

5.709 ± 0.317

2.909 ± 0.212

3.584 ± 0.179

3.767 ± 0.288

6.357 ± 0.385

6.566 ± 0.375

6.119 ± 0.301

1.167 ± 0.169

3.639 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski