Alistipes communis
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2673 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y1WQD6|A0A4Y1WQD6_9BACT ATPase OS=Alistipes communis OX=2585118 GN=A5CBH24_00470 PE=4 SV=1
MM1 pKa = 7.46 LLVALTGMGLASCSEE16 pKa = 4.27 SPEE19 pKa = 4.05 APEE22 pKa = 5.82 DD23 pKa = 3.66 PTEE26 pKa = 4.53 NPDD29 pKa = 5.55 DD30 pKa = 4.09 EE31 pKa = 5.66 DD32 pKa = 5.18 KK33 pKa = 11.75 GDD35 pKa = 3.62 GSEE38 pKa = 5.04 GEE40 pKa = 4.56 GGDD43 pKa = 3.55 EE44 pKa = 4.3 NKK46 pKa = 9.67 TVEE49 pKa = 4.23 LNYY52 pKa = 10.01 TVAEE56 pKa = 3.91 AAYY59 pKa = 9.14 WGDD62 pKa = 4.69 DD63 pKa = 3.46 YY64 pKa = 11.76 MEE66 pKa = 6.13 DD67 pKa = 3.55 GTSNLIVYY75 pKa = 10.01 LYY77 pKa = 10.1 DD78 pKa = 3.45 SPRR81 pKa = 11.84 DD82 pKa = 3.33 EE83 pKa = 5.86 DD84 pKa = 4.3 GVHH87 pKa = 6.63 TGPKK91 pKa = 9.73 DD92 pKa = 3.31 HH93 pKa = 7.26 FNIDD97 pKa = 3.22 MNLPNFNGGEE107 pKa = 4.04 LLIPARR113 pKa = 11.84 SYY115 pKa = 10.94 PIGEE119 pKa = 4.34 PEE121 pKa = 4.28 VYY123 pKa = 10.41 APNTLEE129 pKa = 4.6 PGFADD134 pKa = 3.48 EE135 pKa = 5.09 FEE137 pKa = 4.74 IGGVLYY143 pKa = 10.84 GDD145 pKa = 4.23 YY146 pKa = 10.98 YY147 pKa = 11.61 GVFHH151 pKa = 6.04 QVIDD155 pKa = 3.73 AEE157 pKa = 4.31 QNEE160 pKa = 4.73 TYY162 pKa = 11.19 EE163 pKa = 4.25 MVSEE167 pKa = 4.58 GEE169 pKa = 4.03 MTVGYY174 pKa = 10.2 EE175 pKa = 3.86 NGVYY179 pKa = 10.19 TIDD182 pKa = 3.3 ATFTTEE188 pKa = 3.62 EE189 pKa = 4.34 GVVYY193 pKa = 10.38 HH194 pKa = 5.5 VTYY197 pKa = 10.51 KK198 pKa = 11.01 GAITVDD204 pKa = 4.08 DD205 pKa = 4.57 EE206 pKa = 5.17 SLGEE210 pKa = 4.4 PDD212 pKa = 5.32 DD213 pKa = 4.39 EE214 pKa = 4.68 DD215 pKa = 3.77 WYY217 pKa = 10.88 STLTDD222 pKa = 3.9 DD223 pKa = 5.38 VEE225 pKa = 4.64 VDD227 pKa = 4.17 ALTQGQLYY235 pKa = 10.63 YY236 pKa = 10.24 MGADD240 pKa = 3.65 DD241 pKa = 5.04 KK242 pKa = 11.8 SSLFGILLGTEE253 pKa = 4.53 GVTLSNGTGTGEE265 pKa = 3.83 LMQFFVNADD274 pKa = 3.31 PDD276 pKa = 3.92 AVKK279 pKa = 10.22 EE280 pKa = 4.12 LPAGKK285 pKa = 10.36 YY286 pKa = 9.91 EE287 pKa = 4.01 IVPRR291 pKa = 11.84 SAPMEE296 pKa = 4.45 PEE298 pKa = 4.18 TVIEE302 pKa = 5.0 GYY304 pKa = 10.37 KK305 pKa = 10.34 EE306 pKa = 4.16 GNQLGGSWYY315 pKa = 10.67 FEE317 pKa = 4.18 LEE319 pKa = 3.92 KK320 pKa = 11.15 NLTVNRR326 pKa = 11.84 APAYY330 pKa = 9.23 EE331 pKa = 4.07 GTVTIGHH338 pKa = 5.96 TEE340 pKa = 3.71 NTLDD344 pKa = 3.51 YY345 pKa = 10.23 TVDD348 pKa = 3.89 FEE350 pKa = 6.05 FYY352 pKa = 10.81 DD353 pKa = 4.09 DD354 pKa = 5.31 NLDD357 pKa = 3.12 EE358 pKa = 5.16 GYY360 pKa = 9.9 IVSGSFSGKK369 pKa = 8.59 MEE371 pKa = 4.5 YY372 pKa = 9.85 IYY374 pKa = 10.96 DD375 pKa = 4.24 AGTLQAKK382 pKa = 9.54 GGRR385 pKa = 11.84 VTAPRR390 pKa = 11.84 RR391 pKa = 11.84 PADD394 pKa = 3.24 KK395 pKa = 9.48 RR396 pKa = 11.84 AARR399 pKa = 11.84 IRR401 pKa = 11.84 RR402 pKa = 3.65
Molecular weight: 44.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.973
Patrickios 0.858
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A4Y1WUB2|A0A4Y1WUB2_9BACT Ribosome-recycling factor OS=Alistipes communis OX=2585118 GN=frr PE=3 SV=1
MM1 pKa = 6.74 EE2 pKa = 5.23 TKK4 pKa = 10.08 LQHH7 pKa = 6.55 PLLGEE12 pKa = 3.77 IVLCQTRR19 pKa = 11.84 RR20 pKa = 11.84 ARR22 pKa = 11.84 RR23 pKa = 11.84 ISLSVRR29 pKa = 11.84 STGGVRR35 pKa = 11.84 LSFPCNVPRR44 pKa = 11.84 ARR46 pKa = 11.84 ALAFLEE52 pKa = 5.03 SKK54 pKa = 9.3 TDD56 pKa = 3.14 WVIRR60 pKa = 11.84 TRR62 pKa = 11.84 DD63 pKa = 3.27 RR64 pKa = 11.84 LAARR68 pKa = 11.84 AAAHH72 pKa = 6.58 PAPAPCDD79 pKa = 3.37 IEE81 pKa = 4.29 ALRR84 pKa = 11.84 SAAKK88 pKa = 10.49 AEE90 pKa = 3.97 LPTRR94 pKa = 11.84 VEE96 pKa = 3.85 EE97 pKa = 4.09 LARR100 pKa = 11.84 LHH102 pKa = 5.82 GFRR105 pKa = 11.84 YY106 pKa = 9.98 GRR108 pKa = 11.84 VTIRR112 pKa = 11.84 ASRR115 pKa = 11.84 TKK117 pKa = 9.64 WGCCTGDD124 pKa = 3.46 NNLSLSLFLMTLPPHH139 pKa = 6.55 LRR141 pKa = 11.84 DD142 pKa = 3.41 FVLLHH147 pKa = 6.02 EE148 pKa = 4.96 LCHH151 pKa = 5.84 TVHH154 pKa = 7.09 HH155 pKa = 6.76 DD156 pKa = 3.51 HH157 pKa = 6.87 SPHH160 pKa = 5.39 FHH162 pKa = 6.92 ALLDD166 pKa = 3.84 RR167 pKa = 11.84 LTGGNEE173 pKa = 3.69 KK174 pKa = 10.54 LLSRR178 pKa = 11.84 EE179 pKa = 3.96 LRR181 pKa = 11.84 SYY183 pKa = 10.64 SIPARR188 pKa = 3.79
Molecular weight: 21.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.297
IPC_protein 10.014
Toseland 10.467
ProMoST 10.467
Dawson 10.57
Bjellqvist 10.335
Wikipedia 10.789
Rodwell 10.628
Grimsley 10.613
Solomon 10.716
Lehninger 10.687
Nozaki 10.54
DTASelect 10.292
Thurlkill 10.482
EMBOSS 10.891
Sillero 10.526
Patrickios 10.394
IPC_peptide 10.716
IPC2_peptide 9.78
IPC2.peptide.svr19 8.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2673
0
2673
959588
41
2342
359.0
40.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.491 ± 0.064
1.311 ± 0.02
5.955 ± 0.034
6.559 ± 0.046
4.15 ± 0.025
7.54 ± 0.04
1.778 ± 0.017
5.546 ± 0.034
4.468 ± 0.045
9.038 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.394 ± 0.017
3.78 ± 0.031
4.117 ± 0.029
2.984 ± 0.024
6.845 ± 0.058
5.683 ± 0.044
5.848 ± 0.042
7.068 ± 0.037
1.282 ± 0.018
4.161 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here