Microvirga lotononidis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4YX74|I4YX74_9RHIZ Uncharacterized protein OS=Microvirga lotononidis OX=864069 GN=MicloDRAFT_00027040 PE=4 SV=1
MM1 pKa = 7.81AIFKK5 pKa = 10.27AFNAAGVGFDD15 pKa = 3.49MSSTNSSGWAFIGAHH30 pKa = 6.22PSVTTDD36 pKa = 3.38LVYY39 pKa = 11.15DD40 pKa = 3.76NGTVAEE46 pKa = 4.34FEE48 pKa = 4.47VDD50 pKa = 3.73GSPLVDD56 pKa = 3.74YY57 pKa = 7.1FTARR61 pKa = 11.84YY62 pKa = 8.29WSDD65 pKa = 3.42GYY67 pKa = 9.05TVIIDD72 pKa = 3.57DD73 pKa = 5.65LYY75 pKa = 11.19YY76 pKa = 10.55EE77 pKa = 4.56NNGADD82 pKa = 3.17ILTIQDD88 pKa = 3.52LDD90 pKa = 4.01LYY92 pKa = 8.13TTVDD96 pKa = 3.47ALQGYY101 pKa = 8.6AWYY104 pKa = 10.78VNLNAGHH111 pKa = 6.53DD112 pKa = 3.66TFYY115 pKa = 11.54GNDD118 pKa = 3.42YY119 pKa = 11.04DD120 pKa = 4.71DD121 pKa = 6.27LIRR124 pKa = 11.84AGAGNDD130 pKa = 3.13VVYY133 pKa = 10.17AYY135 pKa = 10.54DD136 pKa = 4.36GDD138 pKa = 4.64DD139 pKa = 3.43IVYY142 pKa = 10.55GDD144 pKa = 4.7AGADD148 pKa = 3.5DD149 pKa = 6.21LYY151 pKa = 11.5GSWGDD156 pKa = 3.53DD157 pKa = 3.34DD158 pKa = 6.43LYY160 pKa = 11.39GGTGRR165 pKa = 11.84DD166 pKa = 3.42ILNGGAGSDD175 pKa = 3.97YY176 pKa = 11.3LNGGADD182 pKa = 3.68NDD184 pKa = 4.09EE185 pKa = 4.3LTGGSGKK192 pKa = 10.51DD193 pKa = 3.13YY194 pKa = 10.83FVFDD198 pKa = 3.93TRR200 pKa = 11.84PSRR203 pKa = 11.84TNVDD207 pKa = 3.73RR208 pKa = 11.84IVDD211 pKa = 4.22FRR213 pKa = 11.84PVDD216 pKa = 3.63DD217 pKa = 5.71TIMLDD222 pKa = 3.32NQVFTRR228 pKa = 11.84VGRR231 pKa = 11.84DD232 pKa = 2.82GWLSGGAFTTGSGARR247 pKa = 11.84DD248 pKa = 3.26SSDD251 pKa = 3.45RR252 pKa = 11.84IIYY255 pKa = 9.28NKK257 pKa = 8.08QTGALLYY264 pKa = 10.64DD265 pKa = 3.95PDD267 pKa = 6.31GIGGAAAIKK276 pKa = 9.57FAQLNKK282 pKa = 10.6GLTLTKK288 pKa = 10.57ADD290 pKa = 4.09FFLLL294 pKa = 3.91

Molecular weight: 31.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4Z232|I4Z232_9RHIZ Uncharacterized protein OS=Microvirga lotononidis OX=864069 GN=MicloDRAFT_00010790 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84FRR4 pKa = 11.84RR5 pKa = 11.84NRR7 pKa = 11.84LPPRR11 pKa = 11.84LFALRR16 pKa = 11.84WILLGVTVLIVAALAVGATFF36 pKa = 4.78

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6918

0

6918

1985803

24

3056

287.0

31.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.771 ± 0.037

0.807 ± 0.01

5.508 ± 0.026

5.757 ± 0.027

3.651 ± 0.02

8.437 ± 0.04

2.081 ± 0.015

5.236 ± 0.023

3.285 ± 0.023

10.223 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.014

2.614 ± 0.02

5.336 ± 0.023

3.312 ± 0.019

7.42 ± 0.038

5.719 ± 0.027

5.386 ± 0.025

7.471 ± 0.022

1.377 ± 0.012

2.24 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski