Atopobium vaginae DSM 15829
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1T629|F1T629_9ACTO Phenylalanine--tRNA ligase alpha subunit OS=Atopobium vaginae DSM 15829 OX=525256 GN=pheS PE=3 SV=1
MM1 pKa = 7.09 VGCIITGHH9 pKa = 6.57 GDD11 pKa = 3.7 FAQGLMSALEE21 pKa = 4.42 MIAGPQNATQAVIFHH36 pKa = 7.03 EE37 pKa = 4.96 DD38 pKa = 2.9 EE39 pKa = 4.18 AATFGVRR46 pKa = 11.84 LTHH49 pKa = 7.34 AIDD52 pKa = 3.39 EE53 pKa = 5.08 AIEE56 pKa = 4.06 QFGSVLVCCDD66 pKa = 3.79 LMGGTPFNQAMLAAADD82 pKa = 3.95 KK83 pKa = 10.23 PVEE86 pKa = 4.15 IVTGCNLGMLLEE98 pKa = 4.51 LTSSQSSASSAQEE111 pKa = 3.74 LAQIAQDD118 pKa = 3.43 AGKK121 pKa = 10.31 EE122 pKa = 4.33 SVAHH126 pKa = 6.64 ISPSQISAHH135 pKa = 6.18 TDD137 pKa = 3.13 NQDD140 pKa = 3.29 TLDD143 pKa = 4.14 ISDD146 pKa = 4.43 EE147 pKa = 4.26 GDD149 pKa = 3.69 GII151 pKa = 4.32
Molecular weight: 15.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.706
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.757
EMBOSS 3.808
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|F1T5S3|F1T5S3_9ACTO Sporulation initiation inhibitor protein Soj OS=Atopobium vaginae DSM 15829 OX=525256 GN=soj PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.32 QPNKK9 pKa = 7.93 SKK11 pKa = 10.59 RR12 pKa = 11.84 VKK14 pKa = 8.79 THH16 pKa = 5.08 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 LVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LTVSLL46 pKa = 4.23
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1243
0
1243
419650
39
3715
337.6
37.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.168 ± 0.079
1.799 ± 0.04
5.985 ± 0.069
5.55 ± 0.061
3.921 ± 0.049
6.428 ± 0.064
2.706 ± 0.035
6.312 ± 0.063
5.626 ± 0.084
9.377 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.564 ± 0.039
3.872 ± 0.051
3.796 ± 0.039
4.126 ± 0.049
4.595 ± 0.06
6.777 ± 0.059
6.009 ± 0.061
7.214 ± 0.059
0.917 ± 0.026
3.26 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here