Dehalogenimonas alkenigignens
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1927 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0GKE3|A0A0W0GKE3_9CHLR Uncharacterized protein OS=Dehalogenimonas alkenigignens OX=1217799 GN=DEALK_18410 PE=4 SV=1
MM1 pKa = 7.23 KK2 pKa = 10.3 RR3 pKa = 11.84 VFKK6 pKa = 10.77 KK7 pKa = 9.88 FLVFAGVLAAVAAVLVPALAVPVSAQATNFSLNGYY42 pKa = 8.75 RR43 pKa = 11.84 VTVGGGGAYY52 pKa = 9.97 SAGWTGGNLGNQWAEE67 pKa = 4.41 GEE69 pKa = 4.3 WVPYY73 pKa = 10.3 KK74 pKa = 10.82 LVITNVQTNYY84 pKa = 10.13 PGLANFPDD92 pKa = 3.71 IVLSFDD98 pKa = 3.27 FHH100 pKa = 7.11 DD101 pKa = 4.33 ANGMFVDD108 pKa = 6.12 LIRR111 pKa = 11.84 GIQVGTTDD119 pKa = 3.62 LTDD122 pKa = 3.63 LQGFPTSGGGPQANNIAGAQLGQNDD147 pKa = 4.14 PDD149 pKa = 3.01 EE150 pKa = 4.43 WAWTNFALLNLPNEE164 pKa = 4.29 QVNRR168 pKa = 11.84 SLVPLGDD175 pKa = 4.29 LDD177 pKa = 4.2 VAPGTDD183 pKa = 2.49 KK184 pKa = 11.01 HH185 pKa = 6.61 VFIIHH190 pKa = 7.03 PSDD193 pKa = 3.12 ILGKK197 pKa = 10.79 GGVTVDD203 pKa = 3.14 SNTIVIYY210 pKa = 9.96 YY211 pKa = 8.76 QAHH214 pKa = 6.78 LSRR217 pKa = 11.84 TFVWSNALQDD227 pKa = 5.09 FYY229 pKa = 11.76 ATDD232 pKa = 3.66 ADD234 pKa = 4.07 LAKK237 pKa = 10.25 WGGYY241 pKa = 9.96 LYY243 pKa = 10.22 ATDD246 pKa = 3.74 AWPTSVQNGSGYY258 pKa = 9.93 QPGSSGHH265 pKa = 4.92 MHH267 pKa = 6.98 LEE269 pKa = 4.15 VEE271 pKa = 4.66 GTGQQDD277 pKa = 3.63 VPIPIPPKK285 pKa = 9.89 PVGLIDD291 pKa = 4.26 GYY293 pKa = 11.3 KK294 pKa = 10.33 FEE296 pKa = 6.24 DD297 pKa = 3.99 RR298 pKa = 11.84 DD299 pKa = 4.22 ADD301 pKa = 4.49 HH302 pKa = 7.04 IWDD305 pKa = 4.48 PGEE308 pKa = 3.82 PAIPGWDD315 pKa = 2.52 IHH317 pKa = 6.32 MYY319 pKa = 10.28 SVLEE323 pKa = 3.95 GGIVIDD329 pKa = 3.69 VHH331 pKa = 5.65 EE332 pKa = 4.53 TTDD335 pKa = 3.42 ANGFYY340 pKa = 10.46 SFPNLTEE347 pKa = 4.51 GIWYY351 pKa = 9.39 IGEE354 pKa = 4.22 HH355 pKa = 5.98 VGPGDD360 pKa = 3.75 PPLAGWTQTFPYY372 pKa = 10.27 NVGNVPPGANSSLIDD387 pKa = 3.69 NFPNNIKK394 pKa = 10.5 AAIGPAHH401 pKa = 6.29 LAEE404 pKa = 4.55 WGWIVSLTDD413 pKa = 3.66 AAPMQHH419 pKa = 5.79 NVNFGNVSFATKK431 pKa = 9.85 TGTKK435 pKa = 10.12 FHH437 pKa = 7.4 DD438 pKa = 4.07 LNANGVWDD446 pKa = 3.86 NGEE449 pKa = 4.31 PGLGGWTIYY458 pKa = 10.94 VDD460 pKa = 3.72 YY461 pKa = 10.89 NNNGVFDD468 pKa = 4.42 AATEE472 pKa = 4.13 PSAVTAGDD480 pKa = 3.42 GTYY483 pKa = 10.35 SINNVAPGTWRR494 pKa = 11.84 VRR496 pKa = 11.84 EE497 pKa = 3.95 VLQAGWFASYY507 pKa = 9.22 PATSDD512 pKa = 2.78 AFGRR516 pKa = 11.84 YY517 pKa = 8.92 HH518 pKa = 7.38 EE519 pKa = 4.33 EE520 pKa = 4.12 TFVGGQTYY528 pKa = 10.55 SGNDD532 pKa = 3.2 FGNWTTASKK541 pKa = 10.97 SGMKK545 pKa = 10.27 FEE547 pKa = 5.91 DD548 pKa = 3.6 MNADD552 pKa = 3.42 GDD554 pKa = 4.16 KK555 pKa = 11.0 DD556 pKa = 4.4 AEE558 pKa = 4.35 DD559 pKa = 3.45 TGLAGWVIYY568 pKa = 10.79 VDD570 pKa = 3.95 YY571 pKa = 10.88 NDD573 pKa = 5.36 NGVKK577 pKa = 10.22 DD578 pKa = 3.97 ANEE581 pKa = 4.1 PFATTAADD589 pKa = 3.53 GTYY592 pKa = 9.62 TITGINPGTWKK603 pKa = 10.5 VKK605 pKa = 10.18 EE606 pKa = 4.02 VAQAGWTQSYY616 pKa = 9.25 PVSGYY621 pKa = 10.38 HH622 pKa = 6.58 EE623 pKa = 4.31 EE624 pKa = 4.46 TFTSGAALTGNDD636 pKa = 3.26 FGNWTTAEE644 pKa = 3.82 KK645 pKa = 10.46 SGYY648 pKa = 9.59 KK649 pKa = 10.54 FNDD652 pKa = 3.45 KK653 pKa = 10.75 YY654 pKa = 11.42 DD655 pKa = 3.98 ADD657 pKa = 4.16 GVRR660 pKa = 11.84 DD661 pKa = 3.71 ADD663 pKa = 3.86 GVDD666 pKa = 3.82 NILGNADD673 pKa = 4.3 DD674 pKa = 4.75 EE675 pKa = 4.8 VLLSGWTIRR684 pKa = 11.84 IYY686 pKa = 11.13 EE687 pKa = 4.12 DD688 pKa = 3.47 TNGNGLLDD696 pKa = 3.66 QAEE699 pKa = 4.37 YY700 pKa = 10.96 DD701 pKa = 3.69 AGPLFTDD708 pKa = 3.42 VTDD711 pKa = 3.6 VNGAYY716 pKa = 10.42 SFTVNPGKK724 pKa = 10.88 YY725 pKa = 9.02 IVVEE729 pKa = 4.11 VMQDD733 pKa = 3.16 KK734 pKa = 11.07 LGTPYY739 pKa = 10.21 YY740 pKa = 10.04 QSYY743 pKa = 8.71 PATPVLAGGLNTGTEE758 pKa = 4.07 LLGANGHH765 pKa = 6.27 AVTLVSSQIDD775 pKa = 3.86 NNNHH779 pKa = 6.22 FGNYY783 pKa = 7.39 WPHH786 pKa = 4.62 TTMTLFDD793 pKa = 4.67 FVWEE797 pKa = 4.46 TTADD801 pKa = 3.73 GNVTLTISDD810 pKa = 3.99 TNDD813 pKa = 2.87 GDD815 pKa = 4.36 VNLTDD820 pKa = 3.5 AHH822 pKa = 5.78 VHH824 pKa = 6.05 LLANDD829 pKa = 3.37 VEE831 pKa = 5.05 YY832 pKa = 9.91 PFSPISTPTSGDD844 pKa = 3.33 DD845 pKa = 3.38 GDD847 pKa = 5.67 GIFEE851 pKa = 5.26 PGEE854 pKa = 3.78 TWTWIVYY861 pKa = 7.49 VTITQDD867 pKa = 3.18 TTFSTWGHH875 pKa = 6.02 GEE877 pKa = 4.09 DD878 pKa = 3.91 PFGNTVDD885 pKa = 3.7 YY886 pKa = 9.73 PQYY889 pKa = 8.4 QSEE892 pKa = 4.33 YY893 pKa = 8.84 AEE895 pKa = 4.1 VFVEE899 pKa = 4.28 VNEE902 pKa = 4.13 ATRR905 pKa = 11.84 TQGFWSTHH913 pKa = 4.8 LDD915 pKa = 3.48 FTEE918 pKa = 4.69 MIFDD922 pKa = 4.3 NYY924 pKa = 10.52 TGDD927 pKa = 4.24 EE928 pKa = 4.14 VDD930 pKa = 3.5 NDD932 pKa = 3.45 NVGFIDD938 pKa = 4.63 LGWRR942 pKa = 11.84 QITNINDD949 pKa = 3.23 LMGVFWANNAKK960 pKa = 10.58 NSDD963 pKa = 3.27 GSNRR967 pKa = 11.84 SALCQARR974 pKa = 11.84 IIASNQALAALLNAAMPGGAPLPAGYY1000 pKa = 10.19 SPAEE1004 pKa = 3.64 IAAILGGTDD1013 pKa = 2.81 IPAIKK1018 pKa = 9.85 MLGSVLGAYY1027 pKa = 10.21 NEE1029 pKa = 5.04 DD1030 pKa = 3.78 GDD1032 pKa = 5.8 DD1033 pKa = 3.75 VALDD1037 pKa = 4.78 PILGSTGKK1045 pKa = 10.5 ANPQQAKK1052 pKa = 9.83 ALANIGFADD1061 pKa = 4.31 CNIQTSLVVAALPASVVKK1079 pKa = 8.78 NTPVNITFTLNTVNDD1094 pKa = 3.42 VGAAGGTFTVIASKK1108 pKa = 10.69 DD1109 pKa = 3.55 SKK1111 pKa = 10.99 FPNGANKK1118 pKa = 9.64 TITVGSGAVNDD1129 pKa = 3.83 MPFNGGVSFTPTSAGTWYY1147 pKa = 10.13 IKK1149 pKa = 9.12 ITYY1152 pKa = 10.02 SGDD1155 pKa = 3.1 GEE1157 pKa = 4.65 NYY1159 pKa = 9.52 GGCSVIVTLVVTNSS1173 pKa = 2.91
Molecular weight: 125.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 4.012
IPC_protein 4.05
Toseland 3.834
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.973
Rodwell 3.872
Grimsley 3.732
Solomon 4.037
Lehninger 3.999
Nozaki 4.139
DTASelect 4.406
Thurlkill 3.872
EMBOSS 3.986
Sillero 4.177
Patrickios 1.494
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.031
Protein with the highest isoelectric point:
>tr|A0A0W0GG97|A0A0W0GG97_9CHLR Probable transaldolase OS=Dehalogenimonas alkenigignens OX=1217799 GN=tal PE=3 SV=1
MM1 pKa = 7.83 PSLKK5 pKa = 10.7 AFFTNWWAPMPLRR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 9.64 IYY22 pKa = 10.32 LAFRR26 pKa = 11.84 NNWIKK31 pKa = 10.27 ISRR34 pKa = 11.84 RR35 pKa = 11.84 QSCCGHH41 pKa = 6.75 PGEE44 pKa = 5.14 PGCC47 pKa = 5.73
Molecular weight: 5.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.604
IPC_protein 10.189
Toseland 10.672
ProMoST 10.292
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 11.052
Grimsley 10.789
Solomon 10.862
Lehninger 10.847
Nozaki 10.701
DTASelect 10.423
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.906
IPC_peptide 10.877
IPC2_peptide 9.838
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1927
0
1927
551133
13
2090
286.0
31.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.235 ± 0.069
1.188 ± 0.028
5.089 ± 0.037
6.244 ± 0.062
3.936 ± 0.04
8.132 ± 0.049
1.864 ± 0.023
6.417 ± 0.051
4.791 ± 0.058
10.047 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.408 ± 0.024
3.186 ± 0.041
4.838 ± 0.043
3.022 ± 0.027
6.103 ± 0.057
5.925 ± 0.048
5.375 ± 0.065
7.298 ± 0.044
1.158 ± 0.022
2.744 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here