Corynebacterium stationis
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2428 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A177IEE6|A0A177IEE6_9CORY Peptide deformylase OS=Corynebacterium stationis OX=1705 GN=def PE=3 SV=1
MM1 pKa = 7.23 AQASEE6 pKa = 5.08 DD7 pKa = 3.82 YY8 pKa = 10.93 SSEE11 pKa = 3.7 NPARR15 pKa = 11.84 RR16 pKa = 11.84 KK17 pKa = 10.01 KK18 pKa = 10.49 SLPAIIGGAVVVLVAGYY35 pKa = 9.69 FGIDD39 pKa = 3.34 LTSDD43 pKa = 2.91 NSEE46 pKa = 3.92 NSGASDD52 pKa = 3.32 NTGNSDD58 pKa = 3.57 DD59 pKa = 4.17 SAVAGSDD66 pKa = 2.84 AGTTDD71 pKa = 4.18 GEE73 pKa = 4.56 TCAIASLPDD82 pKa = 3.42 EE83 pKa = 4.65 ASEE86 pKa = 4.19 TRR88 pKa = 11.84 DD89 pKa = 4.13 DD90 pKa = 3.87 ILAGGPYY97 pKa = 10.02 DD98 pKa = 5.4 YY99 pKa = 10.68 PDD101 pKa = 3.23 NDD103 pKa = 3.13 NRR105 pKa = 11.84 HH106 pKa = 5.73 FGNYY110 pKa = 9.31 EE111 pKa = 4.21 GILPEE116 pKa = 4.06 QDD118 pKa = 2.46 SDD120 pKa = 3.63 FYY122 pKa = 11.42 RR123 pKa = 11.84 EE124 pKa = 4.18 YY125 pKa = 10.56 TVEE128 pKa = 4.19 TPGLDD133 pKa = 2.84 HH134 pKa = 7.29 RR135 pKa = 11.84 GQRR138 pKa = 11.84 RR139 pKa = 11.84 IITGGGSEE147 pKa = 4.68 EE148 pKa = 5.42 DD149 pKa = 3.6 PEE151 pKa = 3.9 AWYY154 pKa = 8.41 YY155 pKa = 10.59 TDD157 pKa = 4.09 DD158 pKa = 4.05 HH159 pKa = 7.62 YY160 pKa = 11.78 EE161 pKa = 4.21 SFCEE165 pKa = 4.71 IPDD168 pKa = 3.75 AEE170 pKa = 4.16
Molecular weight: 18.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.605
ProMoST 3.948
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.935
Patrickios 1.125
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A0X8VHV0|A0A0X8VHV0_9CORY ATP synthase subunit c OS=Corynebacterium stationis OX=1705 GN=atpE PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.5 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2428
0
2428
788366
40
3065
324.7
35.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.371 ± 0.061
0.628 ± 0.013
6.066 ± 0.049
6.656 ± 0.054
3.499 ± 0.036
8.178 ± 0.042
2.099 ± 0.024
5.591 ± 0.038
3.547 ± 0.039
9.427 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.024
3.167 ± 0.031
4.801 ± 0.032
3.463 ± 0.033
5.594 ± 0.047
6.108 ± 0.035
5.91 ± 0.03
7.936 ± 0.04
1.363 ± 0.019
2.262 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here