Saezia sanguinis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Saezia

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A433SAC5|A0A433SAC5_9BURK Uncharacterized protein OS=Saezia sanguinis OX=1965230 GN=CUZ56_02761 PE=4 SV=1
MM1 pKa = 7.47FCPRR5 pKa = 11.84CHH7 pKa = 6.56SVQLAVAHH15 pKa = 5.65YY16 pKa = 9.51QDD18 pKa = 4.86IEE20 pKa = 4.07IDD22 pKa = 3.43HH23 pKa = 7.22CEE25 pKa = 3.87QCHH28 pKa = 6.96GIWLDD33 pKa = 3.54KK34 pKa = 11.66NEE36 pKa = 3.97LQKK39 pKa = 10.74IIEE42 pKa = 4.5RR43 pKa = 11.84EE44 pKa = 3.92TADD47 pKa = 3.86RR48 pKa = 11.84NGDD51 pKa = 3.47GDD53 pKa = 4.44SYY55 pKa = 10.96EE56 pKa = 4.16TDD58 pKa = 3.17LGADD62 pKa = 3.53HH63 pKa = 7.26TSNEE67 pKa = 4.23ADD69 pKa = 3.87TLPTDD74 pKa = 4.33LDD76 pKa = 3.77GSAIADD82 pKa = 3.22IGEE85 pKa = 4.29AAGGDD90 pKa = 2.73IWDD93 pKa = 4.46FLGSLADD100 pKa = 3.76LL101 pKa = 4.55

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433SA22|A0A433SA22_9BURK Barstar domain-containing protein OS=Saezia sanguinis OX=1965230 GN=CUZ56_02884 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2987

0

2987

948645

29

3553

317.6

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.213 ± 0.048

1.072 ± 0.018

5.093 ± 0.038

5.37 ± 0.054

3.724 ± 0.037

7.601 ± 0.086

2.322 ± 0.024

5.651 ± 0.043

4.079 ± 0.051

10.219 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.026

3.625 ± 0.058

4.588 ± 0.04

5.185 ± 0.037

5.374 ± 0.048

5.969 ± 0.054

5.508 ± 0.058

7.226 ± 0.044

1.489 ± 0.024

3.0 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski