Amino acid dipepetide frequency for Saezia sanguinis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.515AlaAla: 11.515 ± 0.166
1.273AlaCys: 1.273 ± 0.043
5.273AlaAsp: 5.273 ± 0.081
5.041AlaGlu: 5.041 ± 0.098
3.509AlaPhe: 3.509 ± 0.073
8.291AlaGly: 8.291 ± 0.166
2.235AlaHis: 2.235 ± 0.052
5.325AlaIle: 5.325 ± 0.086
3.792AlaLys: 3.792 ± 0.077
11.476AlaLeu: 11.476 ± 0.122
2.849AlaMet: 2.849 ± 0.056
3.027AlaAsn: 3.027 ± 0.063
4.082AlaPro: 4.082 ± 0.079
6.501AlaGln: 6.501 ± 0.109
5.397AlaArg: 5.397 ± 0.087
5.954AlaSer: 5.954 ± 0.084
5.048AlaThr: 5.048 ± 0.077
7.19AlaVal: 7.19 ± 0.114
1.408AlaTrp: 1.408 ± 0.041
2.939AlaTyr: 2.939 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.036
0.172CysCys: 0.172 ± 0.015
0.547CysAsp: 0.547 ± 0.025
0.564CysGlu: 0.564 ± 0.029
0.432CysPhe: 0.432 ± 0.023
0.985CysGly: 0.985 ± 0.037
0.31CysHis: 0.31 ± 0.022
0.634CysIle: 0.634 ± 0.029
0.368CysLys: 0.368 ± 0.019
1.015CysLeu: 1.015 ± 0.033
0.335CysMet: 0.335 ± 0.019
0.324CysAsn: 0.324 ± 0.02
0.512CysPro: 0.512 ± 0.024
0.464CysGln: 0.464 ± 0.024
0.521CysArg: 0.521 ± 0.028
0.681CysSer: 0.681 ± 0.031
0.563CysThr: 0.563 ± 0.027
0.742CysVal: 0.742 ± 0.029
0.159CysTrp: 0.159 ± 0.014
0.327CysTyr: 0.327 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
5.383AspAla: 5.383 ± 0.08
0.461AspCys: 0.461 ± 0.024
2.636AspAsp: 2.636 ± 0.071
3.38AspGlu: 3.38 ± 0.069
2.058AspPhe: 2.058 ± 0.049
4.054AspGly: 4.054 ± 0.079
1.075AspHis: 1.075 ± 0.04
3.212AspIle: 3.212 ± 0.062
2.146AspLys: 2.146 ± 0.056
4.852AspLeu: 4.852 ± 0.081
1.453AspMet: 1.453 ± 0.04
1.76AspAsn: 1.76 ± 0.05
2.39AspPro: 2.39 ± 0.052
2.104AspGln: 2.104 ± 0.046
2.49AspArg: 2.49 ± 0.052
2.599AspSer: 2.599 ± 0.059
2.637AspThr: 2.637 ± 0.06
3.766AspVal: 3.766 ± 0.074
0.843AspTrp: 0.843 ± 0.032
1.628AspTyr: 1.628 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.025GluAla: 5.025 ± 0.082
0.452GluCys: 0.452 ± 0.022
2.373GluAsp: 2.373 ± 0.056
2.857GluGlu: 2.857 ± 0.073
1.902GluPhe: 1.902 ± 0.051
3.462GluGly: 3.462 ± 0.058
1.502GluHis: 1.502 ± 0.046
3.277GluIle: 3.277 ± 0.064
2.928GluLys: 2.928 ± 0.066
5.66GluLeu: 5.66 ± 0.084
1.465GluMet: 1.465 ± 0.042
2.213GluAsn: 2.213 ± 0.049
2.311GluPro: 2.311 ± 0.057
3.647GluGln: 3.647 ± 0.076
3.413GluArg: 3.413 ± 0.067
2.654GluSer: 2.654 ± 0.056
2.595GluThr: 2.595 ± 0.05
3.801GluVal: 3.801 ± 0.078
0.669GluTrp: 0.669 ± 0.03
1.492GluTyr: 1.492 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.588PheAla: 3.588 ± 0.067
0.513PheCys: 0.513 ± 0.025
2.353PheAsp: 2.353 ± 0.055
2.144PheGlu: 2.144 ± 0.046
1.643PhePhe: 1.643 ± 0.05
2.867PheGly: 2.867 ± 0.064
0.752PheHis: 0.752 ± 0.028
2.359PheIle: 2.359 ± 0.055
1.474PheLys: 1.474 ± 0.044
3.325PheLeu: 3.325 ± 0.083
0.998PheMet: 0.998 ± 0.04
1.525PheAsn: 1.525 ± 0.04
1.485PhePro: 1.485 ± 0.043
1.306PheGln: 1.306 ± 0.038
1.664PheArg: 1.664 ± 0.039
2.698PheSer: 2.698 ± 0.058
2.078PheThr: 2.078 ± 0.054
2.588PheVal: 2.588 ± 0.059
0.584PheTrp: 0.584 ± 0.027
1.291PheTyr: 1.291 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
6.853GlyAla: 6.853 ± 0.127
0.94GlyCys: 0.94 ± 0.037
3.567GlyAsp: 3.567 ± 0.067
3.8GlyGlu: 3.8 ± 0.063
3.197GlyPhe: 3.197 ± 0.06
5.873GlyGly: 5.873 ± 0.159
1.806GlyHis: 1.806 ± 0.05
4.933GlyIle: 4.933 ± 0.089
3.679GlyLys: 3.679 ± 0.069
7.382GlyLeu: 7.382 ± 0.106
2.187GlyMet: 2.187 ± 0.044
2.883GlyAsn: 2.883 ± 0.122
2.223GlyPro: 2.223 ± 0.053
3.782GlyGln: 3.782 ± 0.081
3.856GlyArg: 3.856 ± 0.068
4.88GlySer: 4.88 ± 0.153
4.401GlyThr: 4.401 ± 0.143
5.911GlyVal: 5.911 ± 0.1
1.26GlyTrp: 1.26 ± 0.039
2.599GlyTyr: 2.599 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.295HisAla: 2.295 ± 0.053
0.284HisCys: 0.284 ± 0.019
1.264HisAsp: 1.264 ± 0.041
1.278HisGlu: 1.278 ± 0.038
0.974HisPhe: 0.974 ± 0.031
1.875HisGly: 1.875 ± 0.047
0.625HisHis: 0.625 ± 0.028
1.443HisIle: 1.443 ± 0.04
0.798HisLys: 0.798 ± 0.031
2.215HisLeu: 2.215 ± 0.053
0.597HisMet: 0.597 ± 0.026
0.777HisAsn: 0.777 ± 0.029
1.326HisPro: 1.326 ± 0.045
1.066HisGln: 1.066 ± 0.035
1.066HisArg: 1.066 ± 0.039
1.17HisSer: 1.17 ± 0.038
1.343HisThr: 1.343 ± 0.038
1.573HisVal: 1.573 ± 0.04
0.453HisTrp: 0.453 ± 0.02
0.797HisTyr: 0.797 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.117IleAla: 6.117 ± 0.077
0.677IleCys: 0.677 ± 0.026
3.259IleAsp: 3.259 ± 0.066
3.686IleGlu: 3.686 ± 0.076
1.987IlePhe: 1.987 ± 0.054
4.589IleGly: 4.589 ± 0.092
1.224IleHis: 1.224 ± 0.041
2.866IleIle: 2.866 ± 0.071
2.247IleLys: 2.247 ± 0.058
4.795IleLeu: 4.795 ± 0.082
1.231IleMet: 1.231 ± 0.04
2.34IleAsn: 2.34 ± 0.069
2.701IlePro: 2.701 ± 0.057
2.316IleGln: 2.316 ± 0.058
3.0IleArg: 3.0 ± 0.056
3.665IleSer: 3.665 ± 0.069
3.387IleThr: 3.387 ± 0.076
3.975IleVal: 3.975 ± 0.068
0.743IleTrp: 0.743 ± 0.026
1.7IleTyr: 1.7 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
4.163LysAla: 4.163 ± 0.082
0.287LysCys: 0.287 ± 0.019
2.1LysAsp: 2.1 ± 0.043
2.374LysGlu: 2.374 ± 0.064
1.092LysPhe: 1.092 ± 0.037
2.776LysGly: 2.776 ± 0.06
0.868LysHis: 0.868 ± 0.029
2.15LysIle: 2.15 ± 0.056
2.278LysLys: 2.278 ± 0.081
3.972LysLeu: 3.972 ± 0.072
1.105LysMet: 1.105 ± 0.034
1.683LysAsn: 1.683 ± 0.052
2.217LysPro: 2.217 ± 0.063
1.969LysGln: 1.969 ± 0.048
2.493LysArg: 2.493 ± 0.054
2.223LysSer: 2.223 ± 0.052
2.604LysThr: 2.604 ± 0.065
2.94LysVal: 2.94 ± 0.069
0.456LysTrp: 0.456 ± 0.023
1.036LysTyr: 1.036 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
10.584LeuAla: 10.584 ± 0.144
1.213LeuCys: 1.213 ± 0.035
5.125LeuAsp: 5.125 ± 0.081
5.019LeuGlu: 5.019 ± 0.083
3.795LeuPhe: 3.795 ± 0.084
7.139LeuGly: 7.139 ± 0.098
2.38LeuHis: 2.38 ± 0.058
5.554LeuIle: 5.554 ± 0.086
4.339LeuLys: 4.339 ± 0.077
10.899LeuLeu: 10.899 ± 0.182
2.649LeuMet: 2.649 ± 0.058
3.827LeuAsn: 3.827 ± 0.085
5.334LeuPro: 5.334 ± 0.09
4.915LeuGln: 4.915 ± 0.084
5.693LeuArg: 5.693 ± 0.096
6.776LeuSer: 6.776 ± 0.094
5.683LeuThr: 5.683 ± 0.097
7.137LeuVal: 7.137 ± 0.087
1.418LeuTrp: 1.418 ± 0.042
2.71LeuTyr: 2.71 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.817MetAla: 2.817 ± 0.053
0.213MetCys: 0.213 ± 0.016
1.286MetAsp: 1.286 ± 0.032
1.228MetGlu: 1.228 ± 0.036
0.797MetPhe: 0.797 ± 0.034
1.878MetGly: 1.878 ± 0.055
0.67MetHis: 0.67 ± 0.031
1.273MetIle: 1.273 ± 0.042
1.269MetLys: 1.269 ± 0.037
3.029MetLeu: 3.029 ± 0.061
0.78MetMet: 0.78 ± 0.029
1.141MetAsn: 1.141 ± 0.035
1.442MetPro: 1.442 ± 0.044
1.539MetGln: 1.539 ± 0.042
1.563MetArg: 1.563 ± 0.04
1.716MetSer: 1.716 ± 0.046
1.581MetThr: 1.581 ± 0.043
1.907MetVal: 1.907 ± 0.053
0.241MetTrp: 0.241 ± 0.015
0.568MetTyr: 0.568 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.813AsnAla: 3.813 ± 0.089
0.346AsnCys: 0.346 ± 0.021
1.806AsnAsp: 1.806 ± 0.052
1.781AsnGlu: 1.781 ± 0.046
1.186AsnPhe: 1.186 ± 0.04
3.149AsnGly: 3.149 ± 0.1
0.81AsnHis: 0.81 ± 0.031
2.166AsnIle: 2.166 ± 0.063
1.413AsnLys: 1.413 ± 0.042
3.307AsnLeu: 3.307 ± 0.069
0.965AsnMet: 0.965 ± 0.03
1.545AsnAsn: 1.545 ± 0.058
2.028AsnPro: 2.028 ± 0.051
1.58AsnGln: 1.58 ± 0.049
1.88AsnArg: 1.88 ± 0.047
2.024AsnSer: 2.024 ± 0.071
2.381AsnThr: 2.381 ± 0.069
2.371AsnVal: 2.371 ± 0.063
0.601AsnTrp: 0.601 ± 0.025
1.103AsnTyr: 1.103 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.191ProAla: 5.191 ± 0.097
0.401ProCys: 0.401 ± 0.021
2.93ProAsp: 2.93 ± 0.065
3.202ProGlu: 3.202 ± 0.062
1.697ProPhe: 1.697 ± 0.047
3.312ProGly: 3.312 ± 0.064
1.002ProHis: 1.002 ± 0.036
2.103ProIle: 2.103 ± 0.05
1.557ProLys: 1.557 ± 0.041
4.323ProLeu: 4.323 ± 0.073
1.163ProMet: 1.163 ± 0.034
1.458ProAsn: 1.458 ± 0.041
1.915ProPro: 1.915 ± 0.054
2.7ProGln: 2.7 ± 0.059
1.925ProArg: 1.925 ± 0.047
2.587ProSer: 2.587 ± 0.05
2.178ProThr: 2.178 ± 0.056
4.056ProVal: 4.056 ± 0.078
0.757ProTrp: 0.757 ± 0.029
1.421ProTyr: 1.421 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
6.063GlnAla: 6.063 ± 0.111
0.432GlnCys: 0.432 ± 0.025
2.105GlnAsp: 2.105 ± 0.052
2.692GlnGlu: 2.692 ± 0.068
1.546GlnPhe: 1.546 ± 0.036
3.722GlnGly: 3.722 ± 0.086
1.339GlnHis: 1.339 ± 0.037
2.771GlnIle: 2.771 ± 0.061
2.144GlnLys: 2.144 ± 0.051
5.007GlnLeu: 5.007 ± 0.076
1.402GlnMet: 1.402 ± 0.04
1.878GlnAsn: 1.878 ± 0.05
2.553GlnPro: 2.553 ± 0.065
3.618GlnGln: 3.618 ± 0.085
3.183GlnArg: 3.183 ± 0.075
2.692GlnSer: 2.692 ± 0.062
2.909GlnThr: 2.909 ± 0.059
3.497GlnVal: 3.497 ± 0.068
0.889GlnTrp: 0.889 ± 0.033
1.405GlnTyr: 1.405 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
4.637ArgAla: 4.637 ± 0.076
0.529ArgCys: 0.529 ± 0.025
2.554ArgAsp: 2.554 ± 0.056
3.13ArgGlu: 3.13 ± 0.067
2.372ArgPhe: 2.372 ± 0.052
3.201ArgGly: 3.201 ± 0.063
1.331ArgHis: 1.331 ± 0.044
3.497ArgIle: 3.497 ± 0.07
2.2ArgLys: 2.2 ± 0.052
5.958ArgLeu: 5.958 ± 0.099
1.769ArgMet: 1.769 ± 0.047
1.929ArgAsn: 1.929 ± 0.047
2.216ArgPro: 2.216 ± 0.051
3.104ArgGln: 3.104 ± 0.064
3.226ArgArg: 3.226 ± 0.074
3.001ArgSer: 3.001 ± 0.059
2.553ArgThr: 2.553 ± 0.049
3.639ArgVal: 3.639 ± 0.081
0.93ArgTrp: 0.93 ± 0.04
1.968ArgTyr: 1.968 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.977SerAla: 5.977 ± 0.092
0.61SerCys: 0.61 ± 0.028
3.079SerAsp: 3.079 ± 0.058
3.037SerGlu: 3.037 ± 0.063
2.279SerPhe: 2.279 ± 0.053
5.588SerGly: 5.588 ± 0.157
1.417SerHis: 1.417 ± 0.035
3.288SerIle: 3.288 ± 0.065
2.199SerLys: 2.199 ± 0.051
5.746SerLeu: 5.746 ± 0.083
1.566SerMet: 1.566 ± 0.041
2.246SerAsn: 2.246 ± 0.064
2.579SerPro: 2.579 ± 0.057
2.7SerGln: 2.7 ± 0.051
3.077SerArg: 3.077 ± 0.069
3.998SerSer: 3.998 ± 0.105
3.391SerThr: 3.391 ± 0.086
4.167SerVal: 4.167 ± 0.078
0.864SerTrp: 0.864 ± 0.036
1.878SerTyr: 1.878 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.517ThrAla: 5.517 ± 0.092
0.501ThrCys: 0.501 ± 0.024
2.751ThrAsp: 2.751 ± 0.058
2.57ThrGlu: 2.57 ± 0.057
1.927ThrPhe: 1.927 ± 0.052
4.885ThrGly: 4.885 ± 0.124
1.257ThrHis: 1.257 ± 0.038
2.928ThrIle: 2.928 ± 0.063
1.525ThrLys: 1.525 ± 0.042
6.414ThrLeu: 6.414 ± 0.108
1.206ThrMet: 1.206 ± 0.039
1.621ThrAsn: 1.621 ± 0.051
3.416ThrPro: 3.416 ± 0.07
2.708ThrGln: 2.708 ± 0.056
2.762ThrArg: 2.762 ± 0.053
3.299ThrSer: 3.299 ± 0.067
3.235ThrThr: 3.235 ± 0.095
4.221ThrVal: 4.221 ± 0.086
0.78ThrTrp: 0.78 ± 0.035
1.553ThrTyr: 1.553 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
7.327ValAla: 7.327 ± 0.107
0.889ValCys: 0.889 ± 0.033
3.713ValAsp: 3.713 ± 0.064
3.655ValGlu: 3.655 ± 0.064
2.988ValPhe: 2.988 ± 0.071
4.924ValGly: 4.924 ± 0.086
1.536ValHis: 1.536 ± 0.045
4.297ValIle: 4.297 ± 0.071
2.82ValLys: 2.82 ± 0.066
7.743ValLeu: 7.743 ± 0.099
2.052ValMet: 2.052 ± 0.052
2.624ValAsn: 2.624 ± 0.062
3.183ValPro: 3.183 ± 0.066
3.344ValGln: 3.344 ± 0.064
3.732ValArg: 3.732 ± 0.069
4.44ValSer: 4.44 ± 0.072
4.134ValThr: 4.134 ± 0.08
5.758ValVal: 5.758 ± 0.106
1.035ValTrp: 1.035 ± 0.034
2.066ValTyr: 2.066 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.112TrpAla: 1.112 ± 0.038
0.191TrpCys: 0.191 ± 0.014
0.611TrpAsp: 0.611 ± 0.026
0.612TrpGlu: 0.612 ± 0.027
0.588TrpPhe: 0.588 ± 0.024
1.019TrpGly: 1.019 ± 0.032
0.418TrpHis: 0.418 ± 0.023
0.865TrpIle: 0.865 ± 0.039
0.573TrpLys: 0.573 ± 0.023
2.066TrpLeu: 2.066 ± 0.054
0.453TrpMet: 0.453 ± 0.021
0.462TrpAsn: 0.462 ± 0.024
0.593TrpPro: 0.593 ± 0.029
1.005TrpGln: 1.005 ± 0.036
0.992TrpArg: 0.992 ± 0.033
0.856TrpSer: 0.856 ± 0.035
0.629TrpThr: 0.629 ± 0.028
1.026TrpVal: 1.026 ± 0.035
0.315TrpTrp: 0.315 ± 0.023
0.503TrpTyr: 0.503 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.08TyrAla: 3.08 ± 0.065
0.339TyrCys: 0.339 ± 0.021
1.595TyrAsp: 1.595 ± 0.046
1.649TyrGlu: 1.649 ± 0.043
1.266TyrPhe: 1.266 ± 0.044
2.425TyrGly: 2.425 ± 0.067
0.661TyrHis: 0.661 ± 0.031
1.563TyrIle: 1.563 ± 0.042
1.036TyrLys: 1.036 ± 0.038
3.004TyrLeu: 3.004 ± 0.059
0.713TyrMet: 0.713 ± 0.03
0.989TyrAsn: 0.989 ± 0.035
1.37TyrPro: 1.37 ± 0.042
1.482TyrGln: 1.482 ± 0.044
1.811TyrArg: 1.811 ± 0.052
1.775TyrSer: 1.775 ± 0.057
1.746TyrThr: 1.746 ± 0.043
1.995TyrVal: 1.995 ± 0.056
0.485TyrTrp: 0.485 ± 0.02
1.011TyrTyr: 1.011 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2987 proteins (948645 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski