Bacillariodnavirus LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Baphyvirales; Bacilladnaviridae; Protobacilladnavirus; Marine protobacilladnavirus 1

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TMU4|S5TMU4_9VIRU Uncharacterized protein OS=Bacillariodnavirus LDMD-2013 OX=1379694 PE=4 SV=1
MM1 pKa = 7.45FGYY4 pKa = 9.94FFAILDD10 pKa = 3.76RR11 pKa = 11.84LDD13 pKa = 4.2WICHH17 pKa = 4.45FLFRR21 pKa = 11.84STMLEE26 pKa = 3.53RR27 pKa = 11.84TMVVLEE33 pKa = 4.21RR34 pKa = 11.84NGNRR38 pKa = 11.84VPYY41 pKa = 9.57DD42 pKa = 2.88IHH44 pKa = 6.5ATQCRR49 pKa = 11.84ICEE52 pKa = 3.97LWHH55 pKa = 5.86TLEE58 pKa = 4.46SAPEE62 pKa = 3.86EE63 pKa = 4.02EE64 pKa = 4.58RR65 pKa = 11.84MNRR68 pKa = 11.84AALEE72 pKa = 4.01LCNQKK77 pKa = 9.19IHH79 pKa = 6.63DD80 pKa = 4.41LKK82 pKa = 11.18FLVEE86 pKa = 4.0VDD88 pKa = 5.49HH89 pKa = 8.0DD90 pKa = 4.63DD91 pKa = 3.44FWPILINHH99 pKa = 6.65PNGLRR104 pKa = 11.84RR105 pKa = 11.84CTWVHH110 pKa = 4.76MFEE113 pKa = 3.59WWSIYY118 pKa = 10.07GVEE121 pKa = 4.58CVLEE125 pKa = 4.46EE126 pKa = 4.48IPIVDD131 pKa = 4.84NIQIAEE137 pKa = 4.09PLDD140 pKa = 3.77YY141 pKa = 11.58NEE143 pKa = 5.57DD144 pKa = 3.5GSIGTNTEE152 pKa = 4.06AEE154 pKa = 4.24MCDD157 pKa = 4.17LVAEE161 pKa = 4.59LL162 pKa = 4.87

Molecular weight: 19.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TNB3|S5TNB3_9VIRU Replication-associated protein OS=Bacillariodnavirus LDMD-2013 OX=1379694 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 4.78IDD4 pKa = 3.61KK5 pKa = 10.34TSRR8 pKa = 11.84GVQSGIRR15 pKa = 11.84KK16 pKa = 9.19RR17 pKa = 11.84SNFEE21 pKa = 3.95EE22 pKa = 4.18IQNALQVVANKK33 pKa = 9.44KK34 pKa = 10.22AKK36 pKa = 10.06RR37 pKa = 11.84LDD39 pKa = 3.5YY40 pKa = 11.03AKK42 pKa = 10.39EE43 pKa = 3.9QGYY46 pKa = 9.53HH47 pKa = 6.52PLQLLGDD54 pKa = 3.97AVKK57 pKa = 10.75DD58 pKa = 3.32IGSVFLPEE66 pKa = 3.83QLASGVGGFITAGASEE82 pKa = 4.05PSIYY86 pKa = 10.32HH87 pKa = 5.86KK88 pKa = 10.86VGNVPTNVTEE98 pKa = 4.13KK99 pKa = 10.95DD100 pKa = 3.14AGQLLQSVSDD110 pKa = 3.39IYY112 pKa = 11.21EE113 pKa = 4.14GVGVLEE119 pKa = 4.52AEE121 pKa = 3.96QKK123 pKa = 9.92KK124 pKa = 10.06RR125 pKa = 11.84KK126 pKa = 9.08KK127 pKa = 10.73AEE129 pKa = 3.63AFIANEE135 pKa = 3.99ADD137 pKa = 3.3TSIGEE142 pKa = 4.27FEE144 pKa = 4.22SALAFLRR151 pKa = 11.84NEE153 pKa = 4.18VVPTGDD159 pKa = 3.24MDD161 pKa = 3.76QGEE164 pKa = 4.32LAEE167 pKa = 4.79AVPLPSGKK175 pKa = 9.77KK176 pKa = 9.92RR177 pKa = 11.84IVSKK181 pKa = 10.85KK182 pKa = 8.45KK183 pKa = 7.25TVKK186 pKa = 10.46RR187 pKa = 11.84KK188 pKa = 7.32ATKK191 pKa = 9.89PRR193 pKa = 11.84TTSKK197 pKa = 10.08RR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 8.34VSPVKK205 pKa = 9.46KK206 pKa = 8.91TSGKK210 pKa = 9.47RR211 pKa = 11.84SKK213 pKa = 10.98KK214 pKa = 10.25SVTKK218 pKa = 10.59GSSKK222 pKa = 9.97RR223 pKa = 11.84RR224 pKa = 11.84KK225 pKa = 8.52GNYY228 pKa = 8.59RR229 pKa = 11.84VSPTGKK235 pKa = 9.16RR236 pKa = 11.84QRR238 pKa = 11.84VSS240 pKa = 2.8

Molecular weight: 26.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1191

162

423

297.8

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.213 ± 0.883

1.511 ± 0.588

5.374 ± 0.483

6.549 ± 0.934

4.282 ± 0.462

7.137 ± 0.897

3.61 ± 0.751

5.458 ± 0.482

6.801 ± 1.7

7.053 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.511 ± 0.554

4.786 ± 0.431

4.45 ± 0.381

4.954 ± 0.679

6.297 ± 0.255

6.885 ± 0.839

6.213 ± 0.613

6.297 ± 0.748

2.183 ± 0.78

2.435 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski