Streptococcus phage Javan440

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B858|A0A4D6B858_9CAUD Uncharacterized protein OS=Streptococcus phage Javan440 OX=2548168 GN=Javan440_0040 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.64YY3 pKa = 10.28KK4 pKa = 9.42VTEE7 pKa = 3.93YY8 pKa = 10.73HH9 pKa = 7.06SDD11 pKa = 3.47FQEE14 pKa = 4.37EE15 pKa = 4.28QTGTCEE21 pKa = 4.41LCFGTAWVEE30 pKa = 3.95NGSITVEE37 pKa = 4.07DD38 pKa = 3.91EE39 pKa = 3.92NGIEE43 pKa = 4.03TEE45 pKa = 4.01IYY47 pKa = 8.99LTVWDD52 pKa = 4.05WGDD55 pKa = 3.31YY56 pKa = 9.09DD57 pKa = 4.58TIYY60 pKa = 10.44IDD62 pKa = 3.47NVVNFSAWLQEE73 pKa = 4.15RR74 pKa = 11.84EE75 pKa = 3.74VDD77 pKa = 4.22PIIEE81 pKa = 4.19EE82 pKa = 4.28TEE84 pKa = 3.82PWSWLHH90 pKa = 6.31EE91 pKa = 4.07LVEE94 pKa = 4.62KK95 pKa = 11.11YY96 pKa = 10.58NEE98 pKa = 3.98EE99 pKa = 4.26LEE101 pKa = 4.3DD102 pKa = 4.5DD103 pKa = 4.27GRR105 pKa = 11.84FKK107 pKa = 11.2AKK109 pKa = 10.13SS110 pKa = 3.24

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B381|A0A4D6B381_9CAUD Uncharacterized protein OS=Streptococcus phage Javan440 OX=2548168 GN=Javan440_0053 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 8.46ITLDD7 pKa = 3.27QAKK10 pKa = 9.37IDD12 pKa = 3.69MYY14 pKa = 11.24INLLEE19 pKa = 4.57RR20 pKa = 11.84GAVDD24 pKa = 3.94FSWVHH29 pKa = 5.65KK30 pKa = 9.48RR31 pKa = 11.84WKK33 pKa = 10.52DD34 pKa = 3.56RR35 pKa = 11.84VRR37 pKa = 11.84KK38 pKa = 7.78EE39 pKa = 3.63LKK41 pKa = 10.19RR42 pKa = 11.84RR43 pKa = 11.84GLSHH47 pKa = 7.65LANN50 pKa = 4.05

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12160

37

1526

173.7

19.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.163 ± 0.563

0.419 ± 0.081

6.431 ± 0.279

8.15 ± 0.358

4.104 ± 0.32

6.061 ± 0.345

1.546 ± 0.111

6.266 ± 0.344

8.512 ± 0.402

9.054 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.189

5.444 ± 0.238

2.533 ± 0.187

4.104 ± 0.23

4.811 ± 0.324

6.168 ± 0.406

5.781 ± 0.291

6.012 ± 0.234

1.118 ± 0.127

3.676 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski