Amino acid dipepetide frequency for Streptococcus phage Javan440

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.208AlaAla: 3.208 ± 1.159
0.493AlaCys: 0.493 ± 0.201
5.017AlaAsp: 5.017 ± 0.637
5.839AlaGlu: 5.839 ± 0.596
2.55AlaPhe: 2.55 ± 0.417
4.441AlaGly: 4.441 ± 0.808
0.658AlaHis: 0.658 ± 0.207
4.935AlaIle: 4.935 ± 0.826
6.086AlaLys: 6.086 ± 1.155
7.155AlaLeu: 7.155 ± 0.964
2.961AlaMet: 2.961 ± 0.588
4.606AlaAsn: 4.606 ± 0.81
1.151AlaPro: 1.151 ± 0.305
2.55AlaGln: 2.55 ± 0.466
3.536AlaArg: 3.536 ± 0.672
4.359AlaSer: 4.359 ± 0.665
3.948AlaThr: 3.948 ± 0.594
5.428AlaVal: 5.428 ± 0.669
0.987AlaTrp: 0.987 ± 0.294
1.727AlaTyr: 1.727 ± 0.447
0.0AlaXaa: 0.0 ± 0.0
Cys
0.164CysAla: 0.164 ± 0.119
0.0CysCys: 0.0 ± 0.0
0.247CysAsp: 0.247 ± 0.119
0.329CysGlu: 0.329 ± 0.159
0.082CysPhe: 0.082 ± 0.096
0.493CysGly: 0.493 ± 0.189
0.082CysHis: 0.082 ± 0.08
0.082CysIle: 0.082 ± 0.093
0.658CysLys: 0.658 ± 0.216
0.493CysLeu: 0.493 ± 0.217
0.082CysMet: 0.082 ± 0.089
0.082CysAsn: 0.082 ± 0.08
0.082CysPro: 0.082 ± 0.083
0.164CysGln: 0.164 ± 0.107
0.493CysArg: 0.493 ± 0.193
0.082CysSer: 0.082 ± 0.08
0.164CysThr: 0.164 ± 0.116
0.247CysVal: 0.247 ± 0.132
0.0CysTrp: 0.0 ± 0.0
0.164CysTyr: 0.164 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
4.77AspAla: 4.77 ± 0.866
0.329AspCys: 0.329 ± 0.153
3.372AspAsp: 3.372 ± 0.729
4.935AspGlu: 4.935 ± 0.585
3.536AspPhe: 3.536 ± 0.418
3.865AspGly: 3.865 ± 0.731
0.822AspHis: 0.822 ± 0.248
4.606AspIle: 4.606 ± 0.626
6.004AspLys: 6.004 ± 0.691
5.675AspLeu: 5.675 ± 0.842
1.974AspMet: 1.974 ± 0.344
3.701AspAsn: 3.701 ± 0.621
1.727AspPro: 1.727 ± 0.334
1.398AspGln: 1.398 ± 0.342
2.467AspArg: 2.467 ± 0.432
2.467AspSer: 2.467 ± 0.414
3.29AspThr: 3.29 ± 0.588
4.852AspVal: 4.852 ± 0.617
0.905AspTrp: 0.905 ± 0.239
3.619AspTyr: 3.619 ± 0.778
0.0AspXaa: 0.0 ± 0.0
Glu
5.264GluAla: 5.264 ± 0.86
0.493GluCys: 0.493 ± 0.204
5.51GluAsp: 5.51 ± 1.038
6.744GluGlu: 6.744 ± 0.837
3.619GluPhe: 3.619 ± 0.721
3.701GluGly: 3.701 ± 0.569
0.411GluHis: 0.411 ± 0.197
5.264GluIle: 5.264 ± 0.693
7.813GluLys: 7.813 ± 0.905
8.307GluLeu: 8.307 ± 0.932
2.632GluMet: 2.632 ± 0.472
5.099GluAsn: 5.099 ± 0.608
1.645GluPro: 1.645 ± 0.474
3.619GluGln: 3.619 ± 0.725
3.619GluArg: 3.619 ± 0.608
5.017GluSer: 5.017 ± 0.757
3.948GluThr: 3.948 ± 0.538
6.168GluVal: 6.168 ± 0.778
0.822GluTrp: 0.822 ± 0.256
1.727GluTyr: 1.727 ± 0.462
0.0GluXaa: 0.0 ± 0.0
Phe
3.372PheAla: 3.372 ± 0.616
0.082PheCys: 0.082 ± 0.075
3.536PheAsp: 3.536 ± 0.551
3.29PheGlu: 3.29 ± 0.559
1.234PhePhe: 1.234 ± 0.272
2.714PheGly: 2.714 ± 0.421
0.74PheHis: 0.74 ± 0.221
2.303PheIle: 2.303 ± 0.486
2.879PheLys: 2.879 ± 0.522
3.948PheLeu: 3.948 ± 0.655
1.398PheMet: 1.398 ± 0.385
2.138PheAsn: 2.138 ± 0.432
1.069PhePro: 1.069 ± 0.273
1.398PheGln: 1.398 ± 0.358
1.892PheArg: 1.892 ± 0.465
2.467PheSer: 2.467 ± 0.44
2.796PheThr: 2.796 ± 0.413
1.645PheVal: 1.645 ± 0.39
0.247PheTrp: 0.247 ± 0.127
1.892PheTyr: 1.892 ± 0.422
0.0PheXaa: 0.0 ± 0.0
Gly
4.277GlyAla: 4.277 ± 0.885
0.247GlyCys: 0.247 ± 0.135
3.619GlyAsp: 3.619 ± 0.544
4.359GlyGlu: 4.359 ± 0.531
4.277GlyPhe: 4.277 ± 0.531
4.112GlyGly: 4.112 ± 0.682
0.658GlyHis: 0.658 ± 0.185
3.372GlyIle: 3.372 ± 0.535
4.359GlyLys: 4.359 ± 0.561
5.428GlyLeu: 5.428 ± 0.856
2.056GlyMet: 2.056 ± 0.44
2.879GlyAsn: 2.879 ± 0.568
0.74GlyPro: 0.74 ± 0.23
3.29GlyGln: 3.29 ± 0.616
3.372GlyArg: 3.372 ± 0.521
3.208GlySer: 3.208 ± 0.66
2.961GlyThr: 2.961 ± 0.523
3.125GlyVal: 3.125 ± 0.453
0.822GlyTrp: 0.822 ± 0.329
3.454GlyTyr: 3.454 ± 0.569
0.0GlyXaa: 0.0 ± 0.0
His
1.234HisAla: 1.234 ± 0.246
0.082HisCys: 0.082 ± 0.072
0.658HisAsp: 0.658 ± 0.189
1.151HisGlu: 1.151 ± 0.284
0.576HisPhe: 0.576 ± 0.235
1.398HisGly: 1.398 ± 0.293
0.411HisHis: 0.411 ± 0.24
0.905HisIle: 0.905 ± 0.277
0.987HisLys: 0.987 ± 0.235
1.398HisLeu: 1.398 ± 0.365
0.082HisMet: 0.082 ± 0.068
0.822HisAsn: 0.822 ± 0.241
0.74HisPro: 0.74 ± 0.198
0.822HisGln: 0.822 ± 0.213
0.411HisArg: 0.411 ± 0.197
1.398HisSer: 1.398 ± 0.43
0.658HisThr: 0.658 ± 0.189
0.822HisVal: 0.822 ± 0.295
0.164HisTrp: 0.164 ± 0.109
0.74HisTyr: 0.74 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
4.77IleAla: 4.77 ± 0.817
0.411IleCys: 0.411 ± 0.165
4.606IleAsp: 4.606 ± 0.609
5.839IleGlu: 5.839 ± 0.83
2.467IlePhe: 2.467 ± 0.453
3.701IleGly: 3.701 ± 0.582
0.74IleHis: 0.74 ± 0.248
3.29IleIle: 3.29 ± 0.64
5.346IleLys: 5.346 ± 0.62
5.51IleLeu: 5.51 ± 0.712
0.987IleMet: 0.987 ± 0.371
2.632IleAsn: 2.632 ± 0.397
1.316IlePro: 1.316 ± 0.397
3.29IleGln: 3.29 ± 0.524
3.043IleArg: 3.043 ± 0.53
4.523IleSer: 4.523 ± 0.667
3.865IleThr: 3.865 ± 0.619
2.961IleVal: 2.961 ± 0.418
0.822IleTrp: 0.822 ± 0.292
2.55IleTyr: 2.55 ± 0.444
0.0IleXaa: 0.0 ± 0.0
Lys
6.497LysAla: 6.497 ± 1.018
0.082LysCys: 0.082 ± 0.081
5.099LysAsp: 5.099 ± 0.611
7.484LysGlu: 7.484 ± 1.0
2.55LysPhe: 2.55 ± 0.401
3.783LysGly: 3.783 ± 0.572
1.563LysHis: 1.563 ± 0.3
5.675LysIle: 5.675 ± 0.813
8.224LysLys: 8.224 ± 1.01
7.484LysLeu: 7.484 ± 0.82
2.138LysMet: 2.138 ± 0.343
5.593LysAsn: 5.593 ± 0.587
2.632LysPro: 2.632 ± 0.5
4.77LysGln: 4.77 ± 0.568
3.948LysArg: 3.948 ± 0.705
4.606LysSer: 4.606 ± 0.532
3.619LysThr: 3.619 ± 0.546
5.675LysVal: 5.675 ± 0.733
1.234LysTrp: 1.234 ± 0.316
2.467LysTyr: 2.467 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
7.649LeuAla: 7.649 ± 0.888
0.082LeuCys: 0.082 ± 0.096
7.155LeuAsp: 7.155 ± 0.729
7.813LeuGlu: 7.813 ± 0.792
3.208LeuPhe: 3.208 ± 0.68
5.428LeuGly: 5.428 ± 0.55
0.905LeuHis: 0.905 ± 0.246
5.017LeuIle: 5.017 ± 0.673
8.636LeuLys: 8.636 ± 0.958
8.389LeuLeu: 8.389 ± 1.13
2.221LeuMet: 2.221 ± 0.577
4.523LeuAsn: 4.523 ± 0.525
2.879LeuPro: 2.879 ± 0.627
2.55LeuGln: 2.55 ± 0.568
5.264LeuArg: 5.264 ± 0.614
5.839LeuSer: 5.839 ± 0.879
5.428LeuThr: 5.428 ± 0.595
4.441LeuVal: 4.441 ± 0.587
0.247LeuTrp: 0.247 ± 0.136
2.879LeuTyr: 2.879 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
1.645MetAla: 1.645 ± 0.314
0.082MetCys: 0.082 ± 0.068
1.645MetAsp: 1.645 ± 0.35
2.879MetGlu: 2.879 ± 0.581
0.822MetPhe: 0.822 ± 0.241
1.316MetGly: 1.316 ± 0.35
0.411MetHis: 0.411 ± 0.194
1.727MetIle: 1.727 ± 0.346
2.056MetLys: 2.056 ± 0.502
1.727MetLeu: 1.727 ± 0.432
0.493MetMet: 0.493 ± 0.201
2.056MetAsn: 2.056 ± 0.477
0.493MetPro: 0.493 ± 0.217
0.905MetGln: 0.905 ± 0.21
0.905MetArg: 0.905 ± 0.247
2.138MetSer: 2.138 ± 0.463
2.467MetThr: 2.467 ± 0.413
1.809MetVal: 1.809 ± 0.434
0.247MetTrp: 0.247 ± 0.147
0.658MetTyr: 0.658 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.701AsnAla: 3.701 ± 0.791
0.247AsnCys: 0.247 ± 0.136
2.961AsnAsp: 2.961 ± 0.479
4.359AsnGlu: 4.359 ± 0.637
1.892AsnPhe: 1.892 ± 0.326
4.688AsnGly: 4.688 ± 0.745
1.151AsnHis: 1.151 ± 0.334
3.701AsnIle: 3.701 ± 0.399
4.359AsnLys: 4.359 ± 0.503
4.606AsnLeu: 4.606 ± 0.589
0.905AsnMet: 0.905 ± 0.263
2.961AsnAsn: 2.961 ± 0.361
1.563AsnPro: 1.563 ± 0.325
3.125AsnGln: 3.125 ± 0.567
2.55AsnArg: 2.55 ± 0.519
3.29AsnSer: 3.29 ± 0.575
2.714AsnThr: 2.714 ± 0.394
3.125AsnVal: 3.125 ± 0.573
0.822AsnTrp: 0.822 ± 0.246
1.727AsnTyr: 1.727 ± 0.34
0.0AsnXaa: 0.0 ± 0.0
Pro
1.316ProAla: 1.316 ± 0.264
0.164ProCys: 0.164 ± 0.106
1.974ProAsp: 1.974 ± 0.537
1.398ProGlu: 1.398 ± 0.347
1.563ProPhe: 1.563 ± 0.263
1.151ProGly: 1.151 ± 0.256
0.905ProHis: 0.905 ± 0.28
1.727ProIle: 1.727 ± 0.454
2.221ProLys: 2.221 ± 0.432
2.138ProLeu: 2.138 ± 0.366
0.658ProMet: 0.658 ± 0.205
0.987ProAsn: 0.987 ± 0.288
0.658ProPro: 0.658 ± 0.277
0.74ProGln: 0.74 ± 0.27
1.151ProArg: 1.151 ± 0.362
1.563ProSer: 1.563 ± 0.375
1.645ProThr: 1.645 ± 0.38
2.221ProVal: 2.221 ± 0.409
0.247ProTrp: 0.247 ± 0.149
0.905ProTyr: 0.905 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
3.701GlnAla: 3.701 ± 0.555
0.082GlnCys: 0.082 ± 0.086
2.303GlnAsp: 2.303 ± 0.408
3.948GlnGlu: 3.948 ± 0.557
1.234GlnPhe: 1.234 ± 0.252
2.385GlnGly: 2.385 ± 0.449
0.576GlnHis: 0.576 ± 0.162
2.632GlnIle: 2.632 ± 0.522
3.372GlnLys: 3.372 ± 0.414
3.865GlnLeu: 3.865 ± 0.558
0.905GlnMet: 0.905 ± 0.252
2.632GlnAsn: 2.632 ± 0.437
1.151GlnPro: 1.151 ± 0.336
1.48GlnGln: 1.48 ± 0.323
2.221GlnArg: 2.221 ± 0.526
1.974GlnSer: 1.974 ± 0.382
2.796GlnThr: 2.796 ± 0.503
2.632GlnVal: 2.632 ± 0.404
0.411GlnTrp: 0.411 ± 0.188
0.74GlnTyr: 0.74 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
2.632ArgAla: 2.632 ± 0.433
0.164ArgCys: 0.164 ± 0.097
2.632ArgAsp: 2.632 ± 0.572
4.277ArgGlu: 4.277 ± 0.562
2.796ArgPhe: 2.796 ± 0.556
2.056ArgGly: 2.056 ± 0.415
1.069ArgHis: 1.069 ± 0.277
3.454ArgIle: 3.454 ± 0.546
4.277ArgLys: 4.277 ± 0.667
4.606ArgLeu: 4.606 ± 0.663
1.563ArgMet: 1.563 ± 0.338
2.303ArgAsn: 2.303 ± 0.384
1.398ArgPro: 1.398 ± 0.328
1.892ArgGln: 1.892 ± 0.456
2.879ArgArg: 2.879 ± 0.673
2.385ArgSer: 2.385 ± 0.419
2.796ArgThr: 2.796 ± 0.482
2.056ArgVal: 2.056 ± 0.474
0.658ArgTrp: 0.658 ± 0.217
2.221ArgTyr: 2.221 ± 0.454
0.0ArgXaa: 0.0 ± 0.0
Ser
4.606SerAla: 4.606 ± 0.557
0.247SerCys: 0.247 ± 0.147
4.112SerAsp: 4.112 ± 0.673
4.523SerGlu: 4.523 ± 0.605
2.467SerPhe: 2.467 ± 0.512
4.194SerGly: 4.194 ± 0.649
1.069SerHis: 1.069 ± 0.309
2.796SerIle: 2.796 ± 0.485
4.688SerLys: 4.688 ± 0.726
5.593SerLeu: 5.593 ± 0.677
2.056SerMet: 2.056 ± 0.386
3.043SerAsn: 3.043 ± 0.453
2.303SerPro: 2.303 ± 0.413
2.55SerGln: 2.55 ± 0.573
2.55SerArg: 2.55 ± 0.53
4.606SerSer: 4.606 ± 0.88
3.29SerThr: 3.29 ± 0.653
3.29SerVal: 3.29 ± 0.4
0.822SerTrp: 0.822 ± 0.29
2.879SerTyr: 2.879 ± 0.528
0.0SerXaa: 0.0 ± 0.0
Thr
5.264ThrAla: 5.264 ± 0.92
0.164ThrCys: 0.164 ± 0.125
3.619ThrAsp: 3.619 ± 0.654
3.125ThrGlu: 3.125 ± 0.555
2.467ThrPhe: 2.467 ± 0.475
3.454ThrGly: 3.454 ± 0.614
0.822ThrHis: 0.822 ± 0.215
3.619ThrIle: 3.619 ± 0.458
4.852ThrLys: 4.852 ± 0.594
5.346ThrLeu: 5.346 ± 0.703
1.151ThrMet: 1.151 ± 0.253
2.714ThrAsn: 2.714 ± 0.493
1.809ThrPro: 1.809 ± 0.445
1.892ThrGln: 1.892 ± 0.443
2.303ThrArg: 2.303 ± 0.533
4.277ThrSer: 4.277 ± 0.806
4.359ThrThr: 4.359 ± 0.801
3.619ThrVal: 3.619 ± 0.49
0.905ThrTrp: 0.905 ± 0.236
2.056ThrTyr: 2.056 ± 0.32
0.0ThrXaa: 0.0 ± 0.0
Val
4.112ValAla: 4.112 ± 0.65
0.164ValCys: 0.164 ± 0.112
3.701ValAsp: 3.701 ± 0.549
5.428ValGlu: 5.428 ± 0.724
1.727ValPhe: 1.727 ± 0.325
4.935ValGly: 4.935 ± 0.696
1.563ValHis: 1.563 ± 0.394
3.701ValIle: 3.701 ± 0.541
4.606ValLys: 4.606 ± 0.46
4.852ValLeu: 4.852 ± 0.623
1.48ValMet: 1.48 ± 0.402
3.208ValAsn: 3.208 ± 0.42
1.069ValPro: 1.069 ± 0.256
1.48ValGln: 1.48 ± 0.345
3.043ValArg: 3.043 ± 0.563
4.441ValSer: 4.441 ± 0.566
4.277ValThr: 4.277 ± 0.584
4.03ValVal: 4.03 ± 0.556
0.329ValTrp: 0.329 ± 0.184
1.974ValTyr: 1.974 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
0.658TrpAla: 0.658 ± 0.197
0.082TrpCys: 0.082 ± 0.089
0.493TrpAsp: 0.493 ± 0.194
1.234TrpGlu: 1.234 ± 0.271
0.576TrpPhe: 0.576 ± 0.242
0.576TrpGly: 0.576 ± 0.2
0.082TrpHis: 0.082 ± 0.078
0.822TrpIle: 0.822 ± 0.219
0.822TrpLys: 0.822 ± 0.31
0.905TrpLeu: 0.905 ± 0.252
0.164TrpMet: 0.164 ± 0.109
0.658TrpAsn: 0.658 ± 0.291
0.082TrpPro: 0.082 ± 0.083
0.74TrpGln: 0.74 ± 0.241
0.411TrpArg: 0.411 ± 0.177
0.987TrpSer: 0.987 ± 0.251
0.493TrpThr: 0.493 ± 0.171
0.493TrpVal: 0.493 ± 0.199
0.082TrpTrp: 0.082 ± 0.068
0.822TrpTyr: 0.822 ± 0.49
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.796TyrAla: 2.796 ± 0.536
0.493TyrCys: 0.493 ± 0.174
2.056TyrAsp: 2.056 ± 0.44
2.55TyrGlu: 2.55 ± 0.535
1.398TyrPhe: 1.398 ± 0.29
2.221TyrGly: 2.221 ± 0.546
0.822TyrHis: 0.822 ± 0.227
3.043TyrIle: 3.043 ± 0.584
2.879TyrLys: 2.879 ± 0.42
3.125TyrLeu: 3.125 ± 0.49
0.493TyrMet: 0.493 ± 0.214
1.809TyrAsn: 1.809 ± 0.338
0.822TyrPro: 0.822 ± 0.311
2.385TyrGln: 2.385 ± 0.365
2.056TyrArg: 2.056 ± 0.55
2.056TyrSer: 2.056 ± 0.63
2.303TyrThr: 2.303 ± 0.4
1.48TyrVal: 1.48 ± 0.316
0.411TyrTrp: 0.411 ± 0.171
1.563TyrTyr: 1.563 ± 0.525
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12160 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski