Candidatus Nitromaritima sp. SCGC AAA799-A02

Taxonomy: cellular organisms; Bacteria; Nitrospinae/Tectomicrobia group; Nitrospinae; Nitrospinia; Nitrospinales; Nitrospinaceae; Candidatus Nitromaritima

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6Z0D6|A0A0J6Z0D6_9BACT FtsA domain-containing protein OS=Candidatus Nitromaritima sp. SCGC AAA799-A02 OX=1628278 GN=UR09_00525 PE=4 SV=1
MM1 pKa = 8.05IEE3 pKa = 3.92VLIATVLIVLGLVLYY18 pKa = 10.56AVVSGNIMAKK28 pKa = 9.66NAEE31 pKa = 4.31TNRR34 pKa = 11.84EE35 pKa = 4.17SIAITLAQDD44 pKa = 3.1KK45 pKa = 11.11LEE47 pKa = 4.63EE48 pKa = 4.28IKK50 pKa = 9.88NTALTVDD57 pKa = 4.23LVGADD62 pKa = 3.89GFDD65 pKa = 3.41SPTYY69 pKa = 8.89STDD72 pKa = 2.58WSATPGGEE80 pKa = 4.32VVDD83 pKa = 4.35SEE85 pKa = 4.97GNSGTSSASYY95 pKa = 9.24TRR97 pKa = 11.84TWTITYY103 pKa = 10.03DD104 pKa = 3.58AILYY108 pKa = 8.37YY109 pKa = 10.53FYY111 pKa = 10.64TVNVTVAWNNGNDD124 pKa = 4.03SISLVTYY131 pKa = 10.6ISQQ134 pKa = 3.4

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J7BP20|A0A0J7BP20_9BACT Uncharacterized protein OS=Candidatus Nitromaritima sp. SCGC AAA799-A02 OX=1628278 GN=UR09_01590 PE=4 SV=1
MM1 pKa = 7.69GEE3 pKa = 3.83AQIFRR8 pKa = 11.84IEE10 pKa = 3.85VLIFNLEE17 pKa = 4.01KK18 pKa = 10.82AGGNIRR24 pKa = 11.84FQGWIFRR31 pKa = 11.84YY32 pKa = 9.43PKK34 pKa = 10.31IPWKK38 pKa = 10.34YY39 pKa = 9.34RR40 pKa = 11.84AAATPDD46 pKa = 3.48DD47 pKa = 3.9SRR49 pKa = 11.84RR50 pKa = 11.84PANISVYY57 pKa = 10.18RR58 pKa = 11.84KK59 pKa = 9.76RR60 pKa = 11.84FGLAGRR66 pKa = 11.84GLNN69 pKa = 3.78

Molecular weight: 8.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1139

0

1139

325876

59

1932

286.1

32.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.239 ± 0.078

1.088 ± 0.026

5.355 ± 0.055

6.977 ± 0.086

4.93 ± 0.066

7.164 ± 0.066

2.079 ± 0.033

6.769 ± 0.063

6.552 ± 0.081

10.151 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.032

4.1 ± 0.047

4.446 ± 0.05

3.356 ± 0.041

5.462 ± 0.07

6.228 ± 0.06

4.951 ± 0.061

6.494 ± 0.064

1.221 ± 0.029

2.982 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski