Geobacillus sp. BCO2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; unclassified Geobacillus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1KER1|A0A0N1KER1_9BACI Cytosol aminopeptidase OS=Geobacillus sp. BCO2 OX=1547578 GN=pepA_2 PE=3 SV=1
MM1 pKa = 7.28EE2 pKa = 5.7HH3 pKa = 7.27GDD5 pKa = 3.4RR6 pKa = 11.84HH7 pKa = 5.08ITVVDD12 pKa = 3.13EE13 pKa = 4.8HH14 pKa = 8.52GNEE17 pKa = 4.01QLCEE21 pKa = 3.68ILFTFEE27 pKa = 4.68SDD29 pKa = 4.23DD30 pKa = 3.72FGKK33 pKa = 10.65SYY35 pKa = 11.12VFYY38 pKa = 10.84YY39 pKa = 10.0PVGVEE44 pKa = 5.18AEE46 pKa = 4.4DD47 pKa = 3.8EE48 pKa = 4.4DD49 pKa = 5.74GEE51 pKa = 4.86TEE53 pKa = 4.16VHH55 pKa = 6.12VSAFIPGDD63 pKa = 3.61GNKK66 pKa = 10.1EE67 pKa = 4.0GEE69 pKa = 4.35LLPIEE74 pKa = 4.66TEE76 pKa = 4.37EE77 pKa = 3.97EE78 pKa = 3.3WDD80 pKa = 3.66MIEE83 pKa = 4.42EE84 pKa = 4.17VWNTFCAEE92 pKa = 3.99QEE94 pKa = 4.29EE95 pKa = 4.64EE96 pKa = 4.38DD97 pKa = 5.22DD98 pKa = 4.01EE99 pKa = 4.62

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0I5P9|A0A0N0I5P9_9BACI Bifunctional homocysteine S-methyltransferase/5 10-methylenetetrahydrofolate reductase OS=Geobacillus sp. BCO2 OX=1547578 GN=yitJ_2 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.33QRR4 pKa = 11.84IVLSLWAAASTAAFILNLLGLMQLLPLWATMPLLFLSLLGLVATWNRR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 5.61QFRR56 pKa = 11.84GFPSKK61 pKa = 10.61RR62 pKa = 11.84MRR64 pKa = 11.84LL65 pKa = 3.52

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4649

0

4649

947638

29

1291

203.8

22.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.299 ± 0.049

1.015 ± 0.015

4.755 ± 0.033

7.138 ± 0.044

4.2 ± 0.029

7.218 ± 0.04

2.307 ± 0.022

6.241 ± 0.036

5.509 ± 0.033

9.838 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.02

3.058 ± 0.027

4.302 ± 0.027

3.565 ± 0.025

6.451 ± 0.04

5.204 ± 0.032

4.976 ± 0.028

7.507 ± 0.035

1.318 ± 0.018

3.258 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski