Amino acid dipepetide frequency for Geobacillus sp. BCO2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.312AlaAla: 11.312 ± 0.153
0.962AlaCys: 0.962 ± 0.037
4.449AlaAsp: 4.449 ± 0.07
6.213AlaGlu: 6.213 ± 0.094
3.777AlaPhe: 3.777 ± 0.073
6.72AlaGly: 6.72 ± 0.092
1.749AlaHis: 1.749 ± 0.04
5.983AlaIle: 5.983 ± 0.08
5.536AlaLys: 5.536 ± 0.08
9.752AlaLeu: 9.752 ± 0.11
2.694AlaMet: 2.694 ± 0.058
2.689AlaAsn: 2.689 ± 0.052
3.337AlaPro: 3.337 ± 0.062
2.508AlaGln: 2.508 ± 0.054
5.183AlaArg: 5.183 ± 0.088
4.799AlaSer: 4.799 ± 0.074
3.715AlaThr: 3.715 ± 0.063
7.909AlaVal: 7.909 ± 0.114
1.029AlaTrp: 1.029 ± 0.036
2.67AlaTyr: 2.67 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.795CysAla: 0.795 ± 0.03
0.268CysCys: 0.268 ± 0.018
0.451CysAsp: 0.451 ± 0.022
0.549CysGlu: 0.549 ± 0.025
0.548CysPhe: 0.548 ± 0.025
0.886CysGly: 0.886 ± 0.033
0.283CysHis: 0.283 ± 0.017
0.502CysIle: 0.502 ± 0.025
0.333CysLys: 0.333 ± 0.021
1.106CysLeu: 1.106 ± 0.039
0.304CysMet: 0.304 ± 0.019
0.246CysAsn: 0.246 ± 0.015
0.485CysPro: 0.485 ± 0.023
0.323CysGln: 0.323 ± 0.018
0.916CysArg: 0.916 ± 0.038
0.633CysSer: 0.633 ± 0.025
0.522CysThr: 0.522 ± 0.025
0.547CysVal: 0.547 ± 0.025
0.151CysTrp: 0.151 ± 0.011
0.301CysTyr: 0.301 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.735AspAla: 3.735 ± 0.061
0.436AspCys: 0.436 ± 0.021
2.638AspAsp: 2.638 ± 0.057
4.615AspGlu: 4.615 ± 0.071
1.844AspPhe: 1.844 ± 0.046
4.086AspGly: 4.086 ± 0.067
1.158AspHis: 1.158 ± 0.034
3.38AspIle: 3.38 ± 0.068
1.926AspLys: 1.926 ± 0.047
4.168AspLeu: 4.168 ± 0.071
1.187AspMet: 1.187 ± 0.034
1.094AspAsn: 1.094 ± 0.034
2.253AspPro: 2.253 ± 0.047
1.378AspGln: 1.378 ± 0.043
3.074AspArg: 3.074 ± 0.061
1.854AspSer: 1.854 ± 0.046
1.682AspThr: 1.682 ± 0.038
4.485AspVal: 4.485 ± 0.079
0.718AspTrp: 0.718 ± 0.027
1.844AspTyr: 1.844 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
7.274GluAla: 7.274 ± 0.102
0.415GluCys: 0.415 ± 0.023
2.599GluAsp: 2.599 ± 0.058
6.354GluGlu: 6.354 ± 0.098
2.044GluPhe: 2.044 ± 0.049
4.138GluGly: 4.138 ± 0.067
1.658GluHis: 1.658 ± 0.044
3.874GluIle: 3.874 ± 0.073
5.115GluLys: 5.115 ± 0.099
6.982GluLeu: 6.982 ± 0.08
2.203GluMet: 2.203 ± 0.05
2.287GluAsn: 2.287 ± 0.049
2.386GluPro: 2.386 ± 0.049
4.215GluGln: 4.215 ± 0.079
6.022GluArg: 6.022 ± 0.098
2.401GluSer: 2.401 ± 0.053
4.047GluThr: 4.047 ± 0.067
4.435GluVal: 4.435 ± 0.074
1.101GluTrp: 1.101 ± 0.034
1.831GluTyr: 1.831 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
4.137PheAla: 4.137 ± 0.077
0.55PheCys: 0.55 ± 0.022
2.056PheAsp: 2.056 ± 0.049
2.064PheGlu: 2.064 ± 0.04
2.238PhePhe: 2.238 ± 0.056
3.315PheGly: 3.315 ± 0.067
1.095PheHis: 1.095 ± 0.036
2.568PheIle: 2.568 ± 0.05
1.242PheLys: 1.242 ± 0.033
4.423PheLeu: 4.423 ± 0.081
1.008PheMet: 1.008 ± 0.035
0.992PheAsn: 0.992 ± 0.038
1.909PhePro: 1.909 ± 0.044
1.412PheGln: 1.412 ± 0.041
2.517PheArg: 2.517 ± 0.055
2.882PheSer: 2.882 ± 0.056
2.089PheThr: 2.089 ± 0.049
3.382PheVal: 3.382 ± 0.069
0.634PheTrp: 0.634 ± 0.024
1.49PheTyr: 1.49 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
6.358GlyAla: 6.358 ± 0.103
0.859GlyCys: 0.859 ± 0.032
3.32GlyAsp: 3.32 ± 0.063
4.849GlyGlu: 4.849 ± 0.074
2.965GlyPhe: 2.965 ± 0.064
5.441GlyGly: 5.441 ± 0.091
1.623GlyHis: 1.623 ± 0.045
4.766GlyIle: 4.766 ± 0.073
4.681GlyLys: 4.681 ± 0.075
6.644GlyLeu: 6.644 ± 0.092
2.421GlyMet: 2.421 ± 0.05
2.115GlyAsn: 2.115 ± 0.048
2.13GlyPro: 2.13 ± 0.049
2.422GlyGln: 2.422 ± 0.054
4.801GlyArg: 4.801 ± 0.074
3.481GlySer: 3.481 ± 0.069
3.869GlyThr: 3.869 ± 0.059
5.731GlyVal: 5.731 ± 0.077
1.035GlyTrp: 1.035 ± 0.032
2.666GlyTyr: 2.666 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.697HisAla: 1.697 ± 0.046
0.293HisCys: 0.293 ± 0.015
1.132HisAsp: 1.132 ± 0.034
1.626HisGlu: 1.626 ± 0.039
1.048HisPhe: 1.048 ± 0.032
1.803HisGly: 1.803 ± 0.048
0.835HisHis: 0.835 ± 0.032
1.438HisIle: 1.438 ± 0.038
0.701HisLys: 0.701 ± 0.029
2.315HisLeu: 2.315 ± 0.054
0.592HisMet: 0.592 ± 0.025
0.632HisAsn: 0.632 ± 0.026
1.524HisPro: 1.524 ± 0.041
0.819HisGln: 0.819 ± 0.033
1.635HisArg: 1.635 ± 0.043
1.086HisSer: 1.086 ± 0.037
0.959HisThr: 0.959 ± 0.03
1.687HisVal: 1.687 ± 0.04
0.342HisTrp: 0.342 ± 0.02
0.901HisTyr: 0.901 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.456IleAla: 6.456 ± 0.087
0.658IleCys: 0.658 ± 0.025
3.87IleAsp: 3.87 ± 0.072
4.892IleGlu: 4.892 ± 0.072
2.119IlePhe: 2.119 ± 0.054
5.482IleGly: 5.482 ± 0.085
1.522IleHis: 1.522 ± 0.044
3.864IleIle: 3.864 ± 0.069
2.523IleLys: 2.523 ± 0.063
4.604IleLeu: 4.604 ± 0.08
1.375IleMet: 1.375 ± 0.042
1.858IleAsn: 1.858 ± 0.055
2.972IlePro: 2.972 ± 0.056
2.085IleGln: 2.085 ± 0.05
4.031IleArg: 4.031 ± 0.066
3.296IleSer: 3.296 ± 0.062
2.875IleThr: 2.875 ± 0.054
5.474IleVal: 5.474 ± 0.067
0.622IleTrp: 0.622 ± 0.026
1.837IleTyr: 1.837 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.812LysAla: 4.812 ± 0.093
0.364LysCys: 0.364 ± 0.021
2.276LysAsp: 2.276 ± 0.057
5.208LysGlu: 5.208 ± 0.076
1.263LysPhe: 1.263 ± 0.038
3.738LysGly: 3.738 ± 0.068
1.067LysHis: 1.067 ± 0.032
2.842LysIle: 2.842 ± 0.063
3.885LysLys: 3.885 ± 0.076
4.465LysLeu: 4.465 ± 0.075
1.662LysMet: 1.662 ± 0.041
1.8LysAsn: 1.8 ± 0.048
2.346LysPro: 2.346 ± 0.053
3.265LysGln: 3.265 ± 0.061
4.498LysArg: 4.498 ± 0.073
2.285LysSer: 2.285 ± 0.056
3.51LysThr: 3.51 ± 0.067
3.446LysVal: 3.446 ± 0.07
0.828LysTrp: 0.828 ± 0.033
1.531LysTyr: 1.531 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
10.203LeuAla: 10.203 ± 0.128
0.982LeuCys: 0.982 ± 0.034
4.792LeuAsp: 4.792 ± 0.073
6.318LeuGlu: 6.318 ± 0.085
4.943LeuPhe: 4.943 ± 0.082
6.7LeuGly: 6.7 ± 0.096
2.279LeuHis: 2.279 ± 0.051
5.553LeuIle: 5.553 ± 0.084
5.069LeuLys: 5.069 ± 0.077
10.65LeuLeu: 10.65 ± 0.147
2.322LeuMet: 2.322 ± 0.052
2.812LeuAsn: 2.812 ± 0.056
4.723LeuPro: 4.723 ± 0.076
3.135LeuGln: 3.135 ± 0.056
5.545LeuArg: 5.545 ± 0.079
6.249LeuSer: 6.249 ± 0.087
4.924LeuThr: 4.924 ± 0.08
6.946LeuVal: 6.946 ± 0.09
1.063LeuTrp: 1.063 ± 0.038
3.175LeuTyr: 3.175 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.952MetAla: 2.952 ± 0.061
0.195MetCys: 0.195 ± 0.015
1.316MetAsp: 1.316 ± 0.035
2.079MetGlu: 2.079 ± 0.049
1.03MetPhe: 1.03 ± 0.037
1.626MetGly: 1.626 ± 0.037
0.509MetHis: 0.509 ± 0.021
1.94MetIle: 1.94 ± 0.042
2.317MetLys: 2.317 ± 0.05
2.45MetLeu: 2.45 ± 0.052
1.024MetMet: 1.024 ± 0.033
1.328MetAsn: 1.328 ± 0.035
1.203MetPro: 1.203 ± 0.039
0.825MetGln: 0.825 ± 0.031
1.631MetArg: 1.631 ± 0.042
1.468MetSer: 1.468 ± 0.038
1.764MetThr: 1.764 ± 0.044
1.76MetVal: 1.76 ± 0.04
0.243MetTrp: 0.243 ± 0.015
0.751MetTyr: 0.751 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.502AsnAla: 2.502 ± 0.053
0.27AsnCys: 0.27 ± 0.016
1.608AsnAsp: 1.608 ± 0.044
2.657AsnGlu: 2.657 ± 0.055
0.946AsnPhe: 0.946 ± 0.034
3.086AsnGly: 3.086 ± 0.068
0.655AsnHis: 0.655 ± 0.023
2.211AsnIle: 2.211 ± 0.049
1.586AsnLys: 1.586 ± 0.041
2.154AsnLeu: 2.154 ± 0.047
0.941AsnMet: 0.941 ± 0.036
1.02AsnAsn: 1.02 ± 0.034
1.66AsnPro: 1.66 ± 0.044
0.988AsnGln: 0.988 ± 0.033
1.902AsnArg: 1.902 ± 0.049
1.305AsnSer: 1.305 ± 0.039
1.297AsnThr: 1.297 ± 0.038
2.523AsnVal: 2.523 ± 0.054
0.382AsnTrp: 0.382 ± 0.02
0.889AsnTyr: 0.889 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
3.408ProAla: 3.408 ± 0.059
0.369ProCys: 0.369 ± 0.02
2.351ProAsp: 2.351 ± 0.052
2.915ProGlu: 2.915 ± 0.062
2.371ProPhe: 2.371 ± 0.051
2.782ProGly: 2.782 ± 0.063
1.182ProHis: 1.182 ± 0.036
2.686ProIle: 2.686 ± 0.055
2.257ProLys: 2.257 ± 0.048
4.828ProLeu: 4.828 ± 0.072
1.077ProMet: 1.077 ± 0.032
1.692ProAsn: 1.692 ± 0.04
1.85ProPro: 1.85 ± 0.05
1.168ProGln: 1.168 ± 0.042
1.945ProArg: 1.945 ± 0.043
2.705ProSer: 2.705 ± 0.055
2.103ProThr: 2.103 ± 0.048
3.26ProVal: 3.26 ± 0.058
0.489ProTrp: 0.489 ± 0.023
1.579ProTyr: 1.579 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.427GlnAla: 3.427 ± 0.072
0.299GlnCys: 0.299 ± 0.019
1.182GlnAsp: 1.182 ± 0.037
2.424GlnGlu: 2.424 ± 0.06
1.5GlnPhe: 1.5 ± 0.036
2.137GlnGly: 2.137 ± 0.046
0.794GlnHis: 0.794 ± 0.029
2.082GlnIle: 2.082 ± 0.054
2.289GlnLys: 2.289 ± 0.047
4.112GlnLeu: 4.112 ± 0.066
1.142GlnMet: 1.142 ± 0.035
1.084GlnAsn: 1.084 ± 0.034
1.374GlnPro: 1.374 ± 0.035
1.74GlnGln: 1.74 ± 0.044
2.286GlnArg: 2.286 ± 0.053
1.672GlnSer: 1.672 ± 0.042
2.146GlnThr: 2.146 ± 0.057
2.074GlnVal: 2.074 ± 0.046
0.628GlnTrp: 0.628 ± 0.029
1.259GlnTyr: 1.259 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
4.695ArgAla: 4.695 ± 0.079
0.838ArgCys: 0.838 ± 0.036
2.747ArgAsp: 2.747 ± 0.052
4.401ArgGlu: 4.401 ± 0.076
2.889ArgPhe: 2.889 ± 0.055
3.739ArgGly: 3.739 ± 0.067
1.791ArgHis: 1.791 ± 0.04
3.611ArgIle: 3.611 ± 0.059
3.663ArgLys: 3.663 ± 0.062
7.467ArgLeu: 7.467 ± 0.089
2.104ArgMet: 2.104 ± 0.056
1.731ArgAsn: 1.731 ± 0.039
2.736ArgPro: 2.736 ± 0.064
2.897ArgGln: 2.897 ± 0.062
5.568ArgArg: 5.568 ± 0.098
3.491ArgSer: 3.491 ± 0.065
3.102ArgThr: 3.102 ± 0.046
3.701ArgVal: 3.701 ± 0.059
1.025ArgTrp: 1.025 ± 0.035
2.316ArgTyr: 2.316 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.606SerAla: 4.606 ± 0.083
0.622SerCys: 0.622 ± 0.028
2.334SerAsp: 2.334 ± 0.05
2.997SerGlu: 2.997 ± 0.064
2.934SerPhe: 2.934 ± 0.053
4.22SerGly: 4.22 ± 0.072
1.107SerHis: 1.107 ± 0.034
3.31SerIle: 3.31 ± 0.065
2.345SerLys: 2.345 ± 0.05
5.57SerLeu: 5.57 ± 0.077
1.493SerMet: 1.493 ± 0.04
1.381SerAsn: 1.381 ± 0.039
2.541SerPro: 2.541 ± 0.06
1.485SerGln: 1.485 ± 0.041
3.053SerArg: 3.053 ± 0.062
3.45SerSer: 3.45 ± 0.065
2.358SerThr: 2.358 ± 0.052
3.911SerVal: 3.911 ± 0.07
0.653SerTrp: 0.653 ± 0.027
1.673SerTyr: 1.673 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
4.664ThrAla: 4.664 ± 0.074
0.448ThrCys: 0.448 ± 0.023
2.356ThrAsp: 2.356 ± 0.058
2.835ThrGlu: 2.835 ± 0.054
2.279ThrPhe: 2.279 ± 0.046
3.928ThrGly: 3.928 ± 0.068
0.976ThrHis: 0.976 ± 0.03
3.746ThrIle: 3.746 ± 0.063
2.968ThrLys: 2.968 ± 0.063
4.617ThrLeu: 4.617 ± 0.073
1.453ThrMet: 1.453 ± 0.036
1.882ThrAsn: 1.882 ± 0.047
2.348ThrPro: 2.348 ± 0.057
1.031ThrGln: 1.031 ± 0.035
2.157ThrArg: 2.157 ± 0.043
2.671ThrSer: 2.671 ± 0.059
2.529ThrThr: 2.529 ± 0.058
4.6ThrVal: 4.6 ± 0.066
0.581ThrTrp: 0.581 ± 0.028
1.686ThrTyr: 1.686 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
6.475ValAla: 6.475 ± 0.102
0.844ValCys: 0.844 ± 0.03
3.705ValAsp: 3.705 ± 0.068
4.859ValGlu: 4.859 ± 0.082
3.221ValPhe: 3.221 ± 0.058
4.933ValGly: 4.933 ± 0.087
1.741ValHis: 1.741 ± 0.043
5.17ValIle: 5.17 ± 0.082
4.38ValLys: 4.38 ± 0.069
7.463ValLeu: 7.463 ± 0.104
2.06ValMet: 2.06 ± 0.039
2.463ValAsn: 2.463 ± 0.049
3.344ValPro: 3.344 ± 0.052
2.391ValGln: 2.391 ± 0.05
4.452ValArg: 4.452 ± 0.063
4.152ValSer: 4.152 ± 0.066
3.938ValThr: 3.938 ± 0.069
5.952ValVal: 5.952 ± 0.103
0.965ValTrp: 0.965 ± 0.031
2.562ValTyr: 2.562 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.93TrpAla: 0.93 ± 0.034
0.125TrpCys: 0.125 ± 0.011
0.5TrpAsp: 0.5 ± 0.026
0.808TrpGlu: 0.808 ± 0.03
0.528TrpPhe: 0.528 ± 0.023
0.722TrpGly: 0.722 ± 0.028
0.298TrpHis: 0.298 ± 0.017
0.825TrpIle: 0.825 ± 0.026
0.916TrpLys: 0.916 ± 0.035
1.789TrpLeu: 1.789 ± 0.053
0.504TrpMet: 0.504 ± 0.021
0.509TrpAsn: 0.509 ± 0.022
0.512TrpPro: 0.512 ± 0.026
0.551TrpGln: 0.551 ± 0.025
1.038TrpArg: 1.038 ± 0.038
0.595TrpSer: 0.595 ± 0.025
0.71TrpThr: 0.71 ± 0.026
0.758TrpVal: 0.758 ± 0.024
0.211TrpTrp: 0.211 ± 0.017
0.354TrpTyr: 0.354 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.548TyrAla: 2.548 ± 0.054
0.35TyrCys: 0.35 ± 0.02
1.871TyrAsp: 1.871 ± 0.046
2.715TyrGlu: 2.715 ± 0.062
1.517TyrPhe: 1.517 ± 0.04
2.695TyrGly: 2.695 ± 0.06
0.746TyrHis: 0.746 ± 0.028
2.063TyrIle: 2.063 ± 0.037
1.357TyrLys: 1.357 ± 0.038
2.827TyrLeu: 2.827 ± 0.057
0.844TyrMet: 0.844 ± 0.031
0.968TyrAsn: 0.968 ± 0.032
1.383TyrPro: 1.383 ± 0.039
1.011TyrGln: 1.011 ± 0.033
2.257TyrArg: 2.257 ± 0.048
1.568TyrSer: 1.568 ± 0.039
1.617TyrThr: 1.617 ± 0.044
2.491TyrVal: 2.491 ± 0.052
0.483TyrTrp: 0.483 ± 0.021
1.269TyrTyr: 1.269 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4649 proteins (947638 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski