Rhodobaca barguzinensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobaca

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8K5S8|A0A2K8K5S8_9RHOB DUF1045 domain-containing protein OS=Rhodobaca barguzinensis OX=441209 GN=BG454_02305 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.35RR3 pKa = 11.84GTLTRR8 pKa = 11.84FVGIWGIFFITPPNVAVSQTSTATFDD34 pKa = 3.3VTIEE38 pKa = 3.89IVAEE42 pKa = 4.22CEE44 pKa = 4.21VTSTQTLDD52 pKa = 3.41FGPAGVLTTEE62 pKa = 4.24IDD64 pKa = 3.3AASDD68 pKa = 3.51IEE70 pKa = 4.84VTCTPDD76 pKa = 2.84VAYY79 pKa = 10.73SIGLDD84 pKa = 3.13EE85 pKa = 4.54GTGDD89 pKa = 3.79GATTSVRR96 pKa = 11.84RR97 pKa = 11.84MTGAGEE103 pKa = 4.2TIDD106 pKa = 3.95YY107 pKa = 11.39GLFQDD112 pKa = 5.05AARR115 pKa = 11.84NVNWGNAPPTDD126 pKa = 3.74TVSATGTGSAQVYY139 pKa = 6.49TVYY142 pKa = 11.02GRR144 pKa = 11.84VPPQATPSEE153 pKa = 4.74GIYY156 pKa = 10.35SDD158 pKa = 4.44TITVTVTYY166 pKa = 10.89

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8K726|A0A2K8K726_9RHOB Uncharacterized protein OS=Rhodobaca barguzinensis OX=441209 GN=BG454_04990 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29AGQKK29 pKa = 9.44ILNARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84MGRR39 pKa = 11.84KK40 pKa = 9.09KK41 pKa = 10.62LSAA44 pKa = 3.93

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3617

0

3617

1148357

29

2646

317.5

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.553 ± 0.051

0.918 ± 0.013

5.829 ± 0.035

5.669 ± 0.038

3.722 ± 0.03

8.474 ± 0.036

2.132 ± 0.021

5.244 ± 0.028

2.728 ± 0.031

10.536 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.02

2.542 ± 0.024

5.119 ± 0.027

3.543 ± 0.021

7.086 ± 0.039

5.193 ± 0.027

5.265 ± 0.03

7.093 ± 0.037

1.42 ± 0.019

2.066 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski