Streptomyces palmae
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z0H6U2|A0A4Z0H6U2_9ACTN DNA-protecting protein DprA OS=Streptomyces palmae OX=1701085 GN=dprA PE=3 SV=1
MM1 pKa = 7.81 VIFDD5 pKa = 4.63 LQNLDD10 pKa = 3.63 VDD12 pKa = 4.68 DD13 pKa = 4.25 KK14 pKa = 11.69 HH15 pKa = 8.74 SGEE18 pKa = 4.38 APASEE23 pKa = 4.68 GSLLTCDD30 pKa = 3.57 MEE32 pKa = 4.72 SAVSILLCGG41 pKa = 3.49
Molecular weight: 4.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.77
IPC_protein 3.605
Toseland 3.427
ProMoST 3.834
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.35
Solomon 3.567
Lehninger 3.528
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.745
Patrickios 1.863
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A4Z0HFP2|A0A4Z0HFP2_9ACTN SDR family oxidoreductase OS=Streptomyces palmae OX=1701085 GN=E4099_02175 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.37 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6120
0
6120
2028746
29
5442
331.5
35.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.964 ± 0.059
0.821 ± 0.009
5.824 ± 0.025
5.938 ± 0.03
2.617 ± 0.017
9.643 ± 0.035
2.346 ± 0.015
3.098 ± 0.02
1.971 ± 0.028
10.403 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.695 ± 0.012
1.599 ± 0.016
6.343 ± 0.033
2.822 ± 0.017
8.469 ± 0.036
4.869 ± 0.024
5.942 ± 0.026
8.158 ± 0.03
1.483 ± 0.013
1.995 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here