Heliocybe sulcata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Gloeophyllales; Gloeophyllales incertae sedis; Heliocybe

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C3NDQ9|A0A5C3NDQ9_9AGAM Glycoside hydrolase family 105 protein OS=Heliocybe sulcata OX=5364 GN=OE88DRAFT_1672797 PE=4 SV=1
DDD2 pKa = 3.37SYYY5 pKa = 10.51GSISIGSPAQQFNVILDDD23 pKa = 3.74GSADDD28 pKa = 3.07WVAGSSCSSCSSSTPTFSTSRR49 pKa = 11.84SSSFQSSTSSAGQQQSLTIRR69 pKa = 11.84YYY71 pKa = 9.1SGQVTGSLGADDD83 pKa = 3.55VQMAGFQVSGQEEE96 pKa = 4.05VVVDDD101 pKa = 3.68TSQNLLSGSNAGIMGLGFQAIASSGATPFWEEE133 pKa = 4.1LVNANQLSQPLMAFWLRR150 pKa = 11.84RR151 pKa = 11.84LLDDD155 pKa = 4.44DD156 pKa = 5.32SATQNSEEE164 pKa = 4.02YYY166 pKa = 10.65GEEE169 pKa = 3.94TLGGTNTSLYYY180 pKa = 9.95GDDD183 pKa = 3.21QFIDDD188 pKa = 4.38PSGVQQSFWLLEEE201 pKa = 4.02QGVSVQGGSVGISTGNAALAAIDDD225 pKa = 3.72GTTLVGGPSNDDD237 pKa = 3.31DDD239 pKa = 4.93IWAAVSGSQALSGQYYY255 pKa = 10.56GFYYY259 pKa = 10.43FPCSTNLQVSLSFGGNAWPISPADDD284 pKa = 3.8NLGAISGNMCLGGIFDDD301 pKa = 4.72TQGADDD307 pKa = 3.27GSGNPGWVVGDDD319 pKa = 3.66FLKKK323 pKa = 10.46VYYY326 pKa = 8.57VFRR329 pKa = 11.84ASPASVGFAQLSEEE343 pKa = 4.13AGGSSGSSPHHH354 pKa = 6.08AASSSSARR362 pKa = 11.84S

Molecular weight: 36.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C3NMN1|A0A5C3NMN1_9AGAM Uncharacterized protein OS=Heliocybe sulcata OX=5364 GN=OE88DRAFT_973144 PE=4 SV=1
MM1 pKa = 7.55PRR3 pKa = 11.84ILNQIVQLLARR14 pKa = 11.84PPTLPRR20 pKa = 11.84PSSAVSALAQLHH32 pKa = 5.34RR33 pKa = 11.84TSLQRR38 pKa = 11.84TTLLPFTSPFQSSSALLAPSFVGQPSALLQLTQVRR73 pKa = 11.84WAARR77 pKa = 11.84GTEE80 pKa = 4.11YY81 pKa = 10.77QPSQRR86 pKa = 11.84VRR88 pKa = 11.84KK89 pKa = 9.28RR90 pKa = 11.84RR91 pKa = 11.84HH92 pKa = 4.84GFLARR97 pKa = 11.84KK98 pKa = 9.55RR99 pKa = 11.84SLHH102 pKa = 4.21GHH104 pKa = 6.86KK105 pKa = 10.1ILARR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84AKK113 pKa = 9.85GRR115 pKa = 11.84KK116 pKa = 8.43FLSHH120 pKa = 6.98

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12554

0

12554

5216656

49

5043

415.5

45.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.806 ± 0.02

1.315 ± 0.009

5.537 ± 0.016

6.043 ± 0.023

3.531 ± 0.014

6.727 ± 0.022

2.488 ± 0.011

4.576 ± 0.014

4.418 ± 0.023

9.22 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.009

3.144 ± 0.011

6.522 ± 0.027

3.673 ± 0.013

6.484 ± 0.02

8.694 ± 0.031

5.841 ± 0.015

6.507 ± 0.018

1.507 ± 0.008

2.784 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski