Vibrio phage KIT04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; unclassified Ermolyevavirinae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A679DMP9|A0A679DMP9_9CAUD Uncharacterized protein OS=Vibrio phage KIT04 OX=2508207 GN=KIT04_025 PE=4 SV=1
MM1 pKa = 7.53FNQKK5 pKa = 10.62DD6 pKa = 3.54KK7 pKa = 11.17TNLVTLDD14 pKa = 3.88YY15 pKa = 10.82ILAEE19 pKa = 4.19LSMLSATDD27 pKa = 4.2DD28 pKa = 4.03PSDD31 pKa = 3.8TVWYY35 pKa = 9.56IDD37 pKa = 3.51SDD39 pKa = 3.83EE40 pKa = 4.47SLLLTVHH47 pKa = 6.83PKK49 pKa = 10.31YY50 pKa = 11.11GLVFYY55 pKa = 10.31NDD57 pKa = 3.38EE58 pKa = 4.04MHH60 pKa = 6.87TLGIYY65 pKa = 10.44DD66 pKa = 4.26KK67 pKa = 9.65EE68 pKa = 4.45QEE70 pKa = 4.22SEE72 pKa = 4.24MLNLLDD78 pKa = 4.87DD79 pKa = 4.29WSICSDD85 pKa = 3.35SKK87 pKa = 9.99VWRR90 pKa = 11.84EE91 pKa = 3.68IGIDD95 pKa = 3.4QIDD98 pKa = 3.93YY99 pKa = 10.91VVV101 pKa = 3.02

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A679DKJ6|A0A679DKJ6_9CAUD Putative exodeoxyribonuclease OS=Vibrio phage KIT04 OX=2508207 GN=KIT04_088 PE=4 SV=1
MM1 pKa = 7.86WDD3 pKa = 3.06RR4 pKa = 11.84HH5 pKa = 4.59KK6 pKa = 11.32LRR8 pKa = 11.84VNWHH12 pKa = 6.33RR13 pKa = 11.84RR14 pKa = 11.84LKK16 pKa = 11.0DD17 pKa = 3.37DD18 pKa = 3.38VTSIEE23 pKa = 3.86NGFNKK28 pKa = 10.18RR29 pKa = 11.84LFRR32 pKa = 11.84QADD35 pKa = 3.65VYY37 pKa = 10.78MYY39 pKa = 10.85DD40 pKa = 4.2LSWWDD45 pKa = 3.51YY46 pKa = 10.8PSRR49 pKa = 11.84TVQRR53 pKa = 11.84SWKK56 pKa = 9.17SQSKK60 pKa = 9.83KK61 pKa = 8.87RR62 pKa = 11.84KK63 pKa = 6.25QWEE66 pKa = 3.91RR67 pKa = 3.3

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

160

0

160

33904

34

1622

211.9

23.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.778 ± 0.437

1.195 ± 0.118

6.303 ± 0.182

7.082 ± 0.264

3.943 ± 0.173

6.454 ± 0.238

1.985 ± 0.114

6.023 ± 0.188

7.61 ± 0.16

8.382 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.139

5.1 ± 0.172

3.386 ± 0.142

3.601 ± 0.149

4.495 ± 0.162

6.129 ± 0.342

6.135 ± 0.215

6.389 ± 0.156

1.413 ± 0.098

3.985 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski