Amino acid dipepetide frequency for Vibrio phage KIT04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.226AlaAla: 7.226 ± 1.574
0.737AlaCys: 0.737 ± 0.154
4.542AlaAsp: 4.542 ± 0.366
5.87AlaGlu: 5.87 ± 0.539
2.507AlaPhe: 2.507 ± 0.285
4.247AlaGly: 4.247 ± 0.379
1.091AlaHis: 1.091 ± 0.163
4.483AlaIle: 4.483 ± 0.381
7.344AlaLys: 7.344 ± 0.833
6.548AlaLeu: 6.548 ± 0.503
2.183AlaMet: 2.183 ± 0.25
3.893AlaAsn: 3.893 ± 0.459
2.271AlaPro: 2.271 ± 0.229
3.038AlaGln: 3.038 ± 0.341
3.156AlaArg: 3.156 ± 0.285
6.106AlaSer: 6.106 ± 1.128
4.837AlaThr: 4.837 ± 0.532
4.07AlaVal: 4.07 ± 0.39
0.973AlaTrp: 0.973 ± 0.154
2.655AlaTyr: 2.655 ± 0.231
0.0AlaXaa: 0.0 ± 0.0
Cys
0.796CysAla: 0.796 ± 0.159
0.118CysCys: 0.118 ± 0.056
0.855CysAsp: 0.855 ± 0.167
0.944CysGlu: 0.944 ± 0.15
0.265CysPhe: 0.265 ± 0.1
0.767CysGly: 0.767 ± 0.16
0.295CysHis: 0.295 ± 0.096
0.619CysIle: 0.619 ± 0.145
0.855CysLys: 0.855 ± 0.179
0.914CysLeu: 0.914 ± 0.19
0.177CysMet: 0.177 ± 0.08
0.56CysAsn: 0.56 ± 0.124
0.472CysPro: 0.472 ± 0.119
0.619CysGln: 0.619 ± 0.133
0.59CysArg: 0.59 ± 0.135
0.855CysSer: 0.855 ± 0.151
0.826CysThr: 0.826 ± 0.154
0.796CysVal: 0.796 ± 0.186
0.118CysTrp: 0.118 ± 0.058
0.501CysTyr: 0.501 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
4.985AspAla: 4.985 ± 0.472
0.678AspCys: 0.678 ± 0.165
3.097AspAsp: 3.097 ± 0.343
4.365AspGlu: 4.365 ± 0.397
2.92AspPhe: 2.92 ± 0.29
4.542AspGly: 4.542 ± 0.622
0.855AspHis: 0.855 ± 0.138
4.631AspIle: 4.631 ± 0.401
4.896AspLys: 4.896 ± 0.32
5.28AspLeu: 5.28 ± 0.423
1.622AspMet: 1.622 ± 0.253
3.363AspAsn: 3.363 ± 0.313
2.065AspPro: 2.065 ± 0.263
1.681AspGln: 1.681 ± 0.246
2.861AspArg: 2.861 ± 0.305
3.628AspSer: 3.628 ± 0.308
3.657AspThr: 3.657 ± 0.363
4.011AspVal: 4.011 ± 0.302
1.327AspTrp: 1.327 ± 0.144
2.566AspTyr: 2.566 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
6.017GluAla: 6.017 ± 0.515
0.855GluCys: 0.855 ± 0.169
4.277GluAsp: 4.277 ± 0.353
4.867GluGlu: 4.867 ± 0.46
2.566GluPhe: 2.566 ± 0.316
3.805GluGly: 3.805 ± 0.334
1.386GluHis: 1.386 ± 0.256
4.454GluIle: 4.454 ± 0.366
5.073GluLys: 5.073 ± 0.417
7.109GluLeu: 7.109 ± 0.723
2.035GluMet: 2.035 ± 0.252
3.392GluAsn: 3.392 ± 0.295
1.799GluPro: 1.799 ± 0.262
3.245GluGln: 3.245 ± 0.331
3.274GluArg: 3.274 ± 0.349
3.599GluSer: 3.599 ± 0.387
3.333GluThr: 3.333 ± 0.4
5.486GluVal: 5.486 ± 0.467
0.885GluTrp: 0.885 ± 0.188
3.363GluTyr: 3.363 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
2.684PheAla: 2.684 ± 0.269
0.619PheCys: 0.619 ± 0.136
3.245PheAsp: 3.245 ± 0.357
3.245PheGlu: 3.245 ± 0.393
1.711PhePhe: 1.711 ± 0.243
2.684PheGly: 2.684 ± 0.284
1.18PheHis: 1.18 ± 0.212
2.183PheIle: 2.183 ± 0.276
2.507PheLys: 2.507 ± 0.227
3.009PheLeu: 3.009 ± 0.295
1.062PheMet: 1.062 ± 0.177
2.271PheAsn: 2.271 ± 0.224
1.504PhePro: 1.504 ± 0.222
0.914PheGln: 0.914 ± 0.171
1.711PheArg: 1.711 ± 0.205
1.976PheSer: 1.976 ± 0.277
2.743PheThr: 2.743 ± 0.3
2.094PheVal: 2.094 ± 0.255
0.678PheTrp: 0.678 ± 0.134
1.416PheTyr: 1.416 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
4.483GlyAla: 4.483 ± 0.482
0.973GlyCys: 0.973 ± 0.196
4.1GlyAsp: 4.1 ± 0.543
4.454GlyGlu: 4.454 ± 0.382
3.245GlyPhe: 3.245 ± 0.351
3.599GlyGly: 3.599 ± 0.335
1.209GlyHis: 1.209 ± 0.188
4.749GlyIle: 4.749 ± 0.37
5.781GlyLys: 5.781 ± 0.486
4.926GlyLeu: 4.926 ± 0.388
1.888GlyMet: 1.888 ± 0.293
3.392GlyAsn: 3.392 ± 0.413
0.501GlyPro: 0.501 ± 0.135
1.976GlyGln: 1.976 ± 0.297
3.009GlyArg: 3.009 ± 0.263
4.188GlySer: 4.188 ± 0.53
4.306GlyThr: 4.306 ± 0.396
3.923GlyVal: 3.923 ± 0.316
1.209GlyTrp: 1.209 ± 0.182
2.625GlyTyr: 2.625 ± 0.276
0.0GlyXaa: 0.0 ± 0.0
His
1.032HisAla: 1.032 ± 0.158
0.383HisCys: 0.383 ± 0.11
1.209HisAsp: 1.209 ± 0.237
1.386HisGlu: 1.386 ± 0.229
0.619HisPhe: 0.619 ± 0.14
1.357HisGly: 1.357 ± 0.213
0.413HisHis: 0.413 ± 0.119
1.209HisIle: 1.209 ± 0.205
1.504HisLys: 1.504 ± 0.179
1.681HisLeu: 1.681 ± 0.245
0.708HisMet: 0.708 ± 0.158
1.18HisAsn: 1.18 ± 0.188
1.003HisPro: 1.003 ± 0.195
0.619HisGln: 0.619 ± 0.133
1.062HisArg: 1.062 ± 0.164
1.091HisSer: 1.091 ± 0.187
1.18HisThr: 1.18 ± 0.207
0.914HisVal: 0.914 ± 0.172
0.324HisTrp: 0.324 ± 0.093
0.973HisTyr: 0.973 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
4.188IleAla: 4.188 ± 0.373
0.619IleCys: 0.619 ± 0.137
4.247IleAsp: 4.247 ± 0.275
5.339IleGlu: 5.339 ± 0.397
1.357IlePhe: 1.357 ± 0.237
3.569IleGly: 3.569 ± 0.362
1.239IleHis: 1.239 ± 0.195
3.569IleIle: 3.569 ± 0.405
4.985IleLys: 4.985 ± 0.374
4.159IleLeu: 4.159 ± 0.331
1.416IleMet: 1.416 ± 0.248
3.422IleAsn: 3.422 ± 0.355
2.655IlePro: 2.655 ± 0.319
2.153IleGln: 2.153 ± 0.23
3.156IleArg: 3.156 ± 0.288
3.687IleSer: 3.687 ± 0.381
4.129IleThr: 4.129 ± 0.404
3.422IleVal: 3.422 ± 0.309
0.59IleTrp: 0.59 ± 0.123
2.33IleTyr: 2.33 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
7.226LysAla: 7.226 ± 0.792
0.796LysCys: 0.796 ± 0.148
4.572LysAsp: 4.572 ± 0.391
5.25LysGlu: 5.25 ± 0.474
3.245LysPhe: 3.245 ± 0.345
4.837LysGly: 4.837 ± 0.359
1.475LysHis: 1.475 ± 0.218
3.952LysIle: 3.952 ± 0.352
4.985LysLys: 4.985 ± 0.395
7.875LysLeu: 7.875 ± 0.567
2.33LysMet: 2.33 ± 0.271
2.773LysAsn: 2.773 ± 0.283
2.92LysPro: 2.92 ± 0.354
2.537LysGln: 2.537 ± 0.307
3.422LysArg: 3.422 ± 0.355
3.982LysSer: 3.982 ± 0.475
4.66LysThr: 4.66 ± 0.4
4.985LysVal: 4.985 ± 0.333
0.855LysTrp: 0.855 ± 0.157
3.422LysTyr: 3.422 ± 0.34
0.0LysXaa: 0.0 ± 0.0
Leu
6.873LeuAla: 6.873 ± 0.56
1.15LeuCys: 1.15 ± 0.186
6.312LeuAsp: 6.312 ± 0.504
6.725LeuGlu: 6.725 ± 0.565
2.537LeuPhe: 2.537 ± 0.282
5.221LeuGly: 5.221 ± 0.43
1.77LeuHis: 1.77 ± 0.268
5.162LeuIle: 5.162 ± 0.411
5.545LeuLys: 5.545 ± 0.523
5.929LeuLeu: 5.929 ± 0.548
2.065LeuMet: 2.065 ± 0.219
3.893LeuAsn: 3.893 ± 0.384
3.481LeuPro: 3.481 ± 0.316
3.038LeuGln: 3.038 ± 0.303
3.716LeuArg: 3.716 ± 0.3
5.486LeuSer: 5.486 ± 0.365
5.516LeuThr: 5.516 ± 0.414
5.87LeuVal: 5.87 ± 0.458
0.737LeuTrp: 0.737 ± 0.206
2.802LeuTyr: 2.802 ± 0.281
0.0LeuXaa: 0.0 ± 0.0
Met
1.888MetAla: 1.888 ± 0.246
0.324MetCys: 0.324 ± 0.102
1.77MetAsp: 1.77 ± 0.243
1.711MetGlu: 1.711 ± 0.24
1.18MetPhe: 1.18 ± 0.208
1.445MetGly: 1.445 ± 0.175
0.442MetHis: 0.442 ± 0.128
1.622MetIle: 1.622 ± 0.217
2.684MetLys: 2.684 ± 0.308
2.006MetLeu: 2.006 ± 0.306
0.649MetMet: 0.649 ± 0.14
1.003MetAsn: 1.003 ± 0.172
1.062MetPro: 1.062 ± 0.206
0.944MetGln: 0.944 ± 0.201
1.003MetArg: 1.003 ± 0.173
1.652MetSer: 1.652 ± 0.201
1.947MetThr: 1.947 ± 0.22
1.239MetVal: 1.239 ± 0.174
0.442MetTrp: 0.442 ± 0.121
1.121MetTyr: 1.121 ± 0.161
0.0MetXaa: 0.0 ± 0.0
Asn
3.893AsnAla: 3.893 ± 0.43
0.619AsnCys: 0.619 ± 0.136
2.094AsnAsp: 2.094 ± 0.25
2.891AsnGlu: 2.891 ± 0.27
1.74AsnPhe: 1.74 ± 0.236
3.481AsnGly: 3.481 ± 0.312
1.003AsnHis: 1.003 ± 0.161
3.481AsnIle: 3.481 ± 0.267
4.631AsnLys: 4.631 ± 0.365
4.69AsnLeu: 4.69 ± 0.356
1.416AsnMet: 1.416 ± 0.206
2.802AsnAsn: 2.802 ± 0.417
2.625AsnPro: 2.625 ± 0.31
1.74AsnGln: 1.74 ± 0.258
2.271AsnArg: 2.271 ± 0.301
2.92AsnSer: 2.92 ± 0.344
3.038AsnThr: 3.038 ± 0.306
3.009AsnVal: 3.009 ± 0.262
0.649AsnTrp: 0.649 ± 0.147
1.976AsnTyr: 1.976 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
1.888ProAla: 1.888 ± 0.263
0.354ProCys: 0.354 ± 0.092
2.212ProAsp: 2.212 ± 0.252
2.92ProGlu: 2.92 ± 0.33
1.416ProPhe: 1.416 ± 0.206
2.124ProGly: 2.124 ± 0.405
0.944ProHis: 0.944 ± 0.17
2.006ProIle: 2.006 ± 0.272
2.183ProLys: 2.183 ± 0.24
2.36ProLeu: 2.36 ± 0.312
0.973ProMet: 0.973 ± 0.165
1.947ProAsn: 1.947 ± 0.287
0.767ProPro: 0.767 ± 0.146
1.18ProGln: 1.18 ± 0.166
1.475ProArg: 1.475 ± 0.2
2.271ProSer: 2.271 ± 0.358
2.33ProThr: 2.33 ± 0.341
2.743ProVal: 2.743 ± 0.282
0.354ProTrp: 0.354 ± 0.094
1.416ProTyr: 1.416 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
3.009GlnAla: 3.009 ± 0.409
0.472GlnCys: 0.472 ± 0.111
1.829GlnAsp: 1.829 ± 0.229
2.714GlnGlu: 2.714 ± 0.298
1.386GlnPhe: 1.386 ± 0.187
2.891GlnGly: 2.891 ± 0.384
0.649GlnHis: 0.649 ± 0.134
1.947GlnIle: 1.947 ± 0.24
2.36GlnLys: 2.36 ± 0.244
3.422GlnLeu: 3.422 ± 0.412
1.15GlnMet: 1.15 ± 0.191
1.563GlnAsn: 1.563 ± 0.261
1.003GlnPro: 1.003 ± 0.177
1.445GlnGln: 1.445 ± 0.248
1.74GlnArg: 1.74 ± 0.223
2.124GlnSer: 2.124 ± 0.296
1.711GlnThr: 1.711 ± 0.182
2.773GlnVal: 2.773 ± 0.302
0.59GlnTrp: 0.59 ± 0.144
1.239GlnTyr: 1.239 ± 0.183
0.0GlnXaa: 0.0 ± 0.0
Arg
3.805ArgAla: 3.805 ± 0.33
0.472ArgCys: 0.472 ± 0.11
3.363ArgAsp: 3.363 ± 0.25
2.743ArgGlu: 2.743 ± 0.258
2.183ArgPhe: 2.183 ± 0.244
3.215ArgGly: 3.215 ± 0.309
1.003ArgHis: 1.003 ± 0.184
2.507ArgIle: 2.507 ± 0.32
3.097ArgLys: 3.097 ± 0.313
3.834ArgLeu: 3.834 ± 0.359
1.18ArgMet: 1.18 ± 0.213
2.596ArgAsn: 2.596 ± 0.291
1.298ArgPro: 1.298 ± 0.193
1.622ArgGln: 1.622 ± 0.185
1.888ArgArg: 1.888 ± 0.274
2.212ArgSer: 2.212 ± 0.256
2.183ArgThr: 2.183 ± 0.273
2.95ArgVal: 2.95 ± 0.344
0.826ArgTrp: 0.826 ± 0.179
1.976ArgTyr: 1.976 ± 0.237
0.0ArgXaa: 0.0 ± 0.0
Ser
5.014SerAla: 5.014 ± 0.949
0.59SerCys: 0.59 ± 0.137
3.156SerAsp: 3.156 ± 0.338
3.923SerGlu: 3.923 ± 0.789
2.448SerPhe: 2.448 ± 0.275
4.955SerGly: 4.955 ± 0.533
1.003SerHis: 1.003 ± 0.172
3.805SerIle: 3.805 ± 0.399
4.778SerLys: 4.778 ± 0.401
5.309SerLeu: 5.309 ± 0.402
1.416SerMet: 1.416 ± 0.199
3.628SerAsn: 3.628 ± 0.433
1.858SerPro: 1.858 ± 0.253
2.153SerGln: 2.153 ± 0.57
2.537SerArg: 2.537 ± 0.278
4.1SerSer: 4.1 ± 0.822
3.657SerThr: 3.657 ± 0.379
3.982SerVal: 3.982 ± 0.333
0.944SerTrp: 0.944 ± 0.178
2.035SerTyr: 2.035 ± 0.265
0.0SerXaa: 0.0 ± 0.0
Thr
4.867ThrAla: 4.867 ± 0.476
0.501ThrCys: 0.501 ± 0.119
3.687ThrAsp: 3.687 ± 0.396
3.893ThrGlu: 3.893 ± 0.348
3.009ThrPhe: 3.009 ± 0.227
4.572ThrGly: 4.572 ± 0.458
1.327ThrHis: 1.327 ± 0.191
3.245ThrIle: 3.245 ± 0.317
4.07ThrLys: 4.07 ± 0.365
4.837ThrLeu: 4.837 ± 0.417
1.091ThrMet: 1.091 ± 0.171
3.097ThrAsn: 3.097 ± 0.4
2.596ThrPro: 2.596 ± 0.264
2.183ThrGln: 2.183 ± 0.308
2.596ThrArg: 2.596 ± 0.271
4.011ThrSer: 4.011 ± 0.521
3.569ThrThr: 3.569 ± 0.313
4.247ThrVal: 4.247 ± 0.327
1.209ThrTrp: 1.209 ± 0.273
2.743ThrTyr: 2.743 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
4.365ValAla: 4.365 ± 0.401
0.914ValCys: 0.914 ± 0.156
4.749ValAsp: 4.749 ± 0.331
4.1ValGlu: 4.1 ± 0.377
3.186ValPhe: 3.186 ± 0.312
3.746ValGly: 3.746 ± 0.351
1.268ValHis: 1.268 ± 0.165
3.009ValIle: 3.009 ± 0.291
5.132ValLys: 5.132 ± 0.326
5.191ValLeu: 5.191 ± 0.417
1.298ValMet: 1.298 ± 0.186
2.861ValAsn: 2.861 ± 0.248
2.301ValPro: 2.301 ± 0.294
2.419ValGln: 2.419 ± 0.248
2.979ValArg: 2.979 ± 0.358
4.336ValSer: 4.336 ± 0.332
4.129ValThr: 4.129 ± 0.416
4.572ValVal: 4.572 ± 0.429
0.708ValTrp: 0.708 ± 0.15
2.625ValTyr: 2.625 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
1.15TrpAla: 1.15 ± 0.198
0.206TrpCys: 0.206 ± 0.069
1.239TrpAsp: 1.239 ± 0.166
0.708TrpGlu: 0.708 ± 0.173
0.531TrpPhe: 0.531 ± 0.131
1.15TrpGly: 1.15 ± 0.178
0.324TrpHis: 0.324 ± 0.102
0.885TrpIle: 0.885 ± 0.218
0.885TrpLys: 0.885 ± 0.151
1.239TrpLeu: 1.239 ± 0.201
0.354TrpMet: 0.354 ± 0.103
0.855TrpAsn: 0.855 ± 0.185
0.295TrpPro: 0.295 ± 0.094
0.56TrpGln: 0.56 ± 0.129
0.737TrpArg: 0.737 ± 0.159
0.944TrpSer: 0.944 ± 0.267
0.531TrpThr: 0.531 ± 0.137
0.944TrpVal: 0.944 ± 0.144
0.118TrpTrp: 0.118 ± 0.059
0.472TrpTyr: 0.472 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.389TyrAla: 2.389 ± 0.226
0.56TyrCys: 0.56 ± 0.136
2.478TyrAsp: 2.478 ± 0.278
2.773TyrGlu: 2.773 ± 0.33
1.386TyrPhe: 1.386 ± 0.218
2.33TyrGly: 2.33 ± 0.247
0.973TyrHis: 0.973 ± 0.17
2.714TyrIle: 2.714 ± 0.339
2.802TyrLys: 2.802 ± 0.315
3.51TyrLeu: 3.51 ± 0.368
1.091TyrMet: 1.091 ± 0.176
2.507TyrAsn: 2.507 ± 0.303
1.386TyrPro: 1.386 ± 0.182
1.947TyrGln: 1.947 ± 0.26
1.77TyrArg: 1.77 ± 0.244
2.124TyrSer: 2.124 ± 0.28
3.068TyrThr: 3.068 ± 0.391
1.858TyrVal: 1.858 ± 0.226
0.59TyrTrp: 0.59 ± 0.154
1.593TyrTyr: 1.593 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 160 proteins (33904 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski