Ochrobactrum phage vB_OspM_OC
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 390 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XXQ3|A0A6G6XXQ3_9CAUD Uncharacterized protein OS=Ochrobactrum phage vB_OspM_OC OX=2712956 GN=phiOC_p225 PE=4 SV=1
MM1 pKa = 7.37 RR2 pKa = 11.84 KK3 pKa = 9.42 KK4 pKa = 10.92 YY5 pKa = 10.41 KK6 pKa = 10.46 FSCPCQDD13 pKa = 3.76 KK14 pKa = 10.57 EE15 pKa = 4.8 VTITWTTDD23 pKa = 3.32 DD24 pKa = 3.96 EE25 pKa = 5.32 DD26 pKa = 4.03 EE27 pKa = 4.67 TVDD30 pKa = 4.2 FCPFCSANILDD41 pKa = 3.94 EE42 pKa = 4.68 DD43 pKa = 4.18 VPLEE47 pKa = 4.01 EE48 pKa = 6.3 DD49 pKa = 3.86 EE50 pKa = 6.04 DD51 pKa = 4.43 VSFYY55 pKa = 11.61 DD56 pKa = 4.06 NDD58 pKa = 3.49
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.439
ProMoST 3.77
Dawson 3.656
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.579
Nozaki 3.783
DTASelect 3.999
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.77
Patrickios 1.85
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A6G6XX96|A0A6G6XX96_9CAUD Uncharacterized protein OS=Ochrobactrum phage vB_OspM_OC OX=2712956 GN=phiOC_p184 PE=4 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 LVKK5 pKa = 10.28 HH6 pKa = 5.64 VSGLRR11 pKa = 11.84 KK12 pKa = 9.49 ASYY15 pKa = 9.04 PYY17 pKa = 10.84 RR18 pKa = 11.84 MWFNKK23 pKa = 8.97 VSNVHH28 pKa = 6.44 AEE30 pKa = 3.85 NGFDD34 pKa = 4.26 SRR36 pKa = 11.84 LL37 pKa = 3.41
Molecular weight: 4.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.97
IPC_protein 10.701
Toseland 10.716
ProMoST 10.409
Dawson 10.833
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.155
Grimsley 10.891
Solomon 10.935
Lehninger 10.906
Nozaki 10.687
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.979
IPC_peptide 10.935
IPC2_peptide 9.341
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
390
0
390
70648
29
1942
181.1
20.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.835 ± 0.148
1.04 ± 0.061
6.697 ± 0.095
6.671 ± 0.199
4.992 ± 0.099
6.38 ± 0.251
2.045 ± 0.085
7.641 ± 0.133
7.383 ± 0.177
7.547 ± 0.121
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.664 ± 0.084
6.237 ± 0.148
3.175 ± 0.071
2.882 ± 0.067
4.207 ± 0.094
6.283 ± 0.144
6.05 ± 0.183
6.415 ± 0.116
1.265 ± 0.054
4.592 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here