Gluconobacter morbifer G707
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2846 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G6XKP4|G6XKP4_9PROT CMD domain-containing protein OS=Gluconobacter morbifer G707 OX=1088869 GN=GMO_20600 PE=4 SV=1
MM1 pKa = 7.69 AGLKK5 pKa = 10.39 SSAHH9 pKa = 6.12 YY10 pKa = 10.92 DD11 pKa = 3.39 LTSGSNSITGGSAAQGLISSGGYY34 pKa = 6.83 YY35 pKa = 9.82 TINGEE40 pKa = 4.32 LGYY43 pKa = 10.03 IAAGSYY49 pKa = 10.89 GDD51 pKa = 4.0 SSNPQDD57 pKa = 3.61 TLNSGVAIDD66 pKa = 4.73 LRR68 pKa = 11.84 NNTASSVSVLAGDD81 pKa = 3.61 QAGVTVYY88 pKa = 10.82 AGDD91 pKa = 3.46 QSGSFVGGLGDD102 pKa = 4.16 NVFLGSGKK110 pKa = 8.31 TGSWNVATGSGNDD123 pKa = 4.06 TILGTNGNSTIDD135 pKa = 3.64 GGTGDD140 pKa = 3.58 NLIYY144 pKa = 10.53 LGSGTNVVRR153 pKa = 11.84 SEE155 pKa = 4.15 GQDD158 pKa = 3.39 TIDD161 pKa = 3.8 GGGGVDD167 pKa = 3.4 TVTLLGGSSVVSLQNNATVYY187 pKa = 8.83 DD188 pKa = 4.14 TTGHH192 pKa = 6.33 NDD194 pKa = 2.99 VTVGSNSSITGGSSSTYY211 pKa = 8.28 FTTGSMSTISGGQNDD226 pKa = 4.98 TISASGDD233 pKa = 3.29 LEE235 pKa = 4.17 QIRR238 pKa = 11.84 GSGNNLSVGGSLTFLNGTGSTTITAGNATLFGASGQDD275 pKa = 2.97 IQYY278 pKa = 9.35 TGTSGTALYY287 pKa = 10.55 VAGDD291 pKa = 3.68 GSEE294 pKa = 4.49 TIDD297 pKa = 3.49 ASASKK302 pKa = 9.03 TAINAFAGTGDD313 pKa = 3.58 DD314 pKa = 4.13 TIIGGSAADD323 pKa = 3.71 TMVGGSGNATLTGGSGAANLFALVDD348 pKa = 4.32 GKK350 pKa = 11.01 AGGDD354 pKa = 3.47 YY355 pKa = 10.49 TITDD359 pKa = 4.54 FGSAAGNLVALYY371 pKa = 10.67 NYY373 pKa = 9.63 GLNSNTLQTVLNDD386 pKa = 3.41 ATVSGGNTTIALSDD400 pKa = 3.36 NSKK403 pKa = 8.49 ITFVGVTDD411 pKa = 4.97 LKK413 pKa = 10.43 TSNFTGG419 pKa = 3.49
Molecular weight: 40.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.579
IPC_protein 3.63
Toseland 3.389
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.643
Rodwell 3.452
Grimsley 3.287
Solomon 3.643
Lehninger 3.605
Nozaki 3.77
DTASelect 4.101
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.757
Patrickios 0.477
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|G6XH41|G6XH41_9PROT Glycerol-3-phosphate dehydrogenase OS=Gluconobacter morbifer G707 OX=1088869 GN=GMO_08060 PE=3 SV=1
MM1 pKa = 7.63 PRR3 pKa = 11.84 PSGFSMPDD11 pKa = 2.99 ALEE14 pKa = 3.6 QRR16 pKa = 11.84 LIGEE20 pKa = 4.73 LTTAVDD26 pKa = 3.6 PAVVAFAHH34 pKa = 6.31 NLAQSMPAPPLGVLFYY50 pKa = 11.45 GSMLRR55 pKa = 11.84 SADD58 pKa = 3.41 PDD60 pKa = 4.65 GILDD64 pKa = 3.9 FYY66 pKa = 10.9 IITEE70 pKa = 4.15 TARR73 pKa = 11.84 DD74 pKa = 3.94 FPGGFLARR82 pKa = 11.84 AANRR86 pKa = 11.84 LLPPNVRR93 pKa = 11.84 YY94 pKa = 10.34 AEE96 pKa = 4.26 FTASDD101 pKa = 3.55 NRR103 pKa = 11.84 ILRR106 pKa = 11.84 AKK108 pKa = 10.11 IAILSRR114 pKa = 11.84 AQFAARR120 pKa = 11.84 TSLASLDD127 pKa = 3.53 TTLWARR133 pKa = 11.84 FCQPARR139 pKa = 11.84 LIWVRR144 pKa = 11.84 GPEE147 pKa = 3.98 AADD150 pKa = 3.48 ALLDD154 pKa = 4.53 LIARR158 pKa = 11.84 CVTTAACWSALLGEE172 pKa = 4.64 PRR174 pKa = 11.84 MTALGFWRR182 pKa = 11.84 TLFAQTYY189 pKa = 8.23 ASEE192 pKa = 4.13 LRR194 pKa = 11.84 VEE196 pKa = 4.32 KK197 pKa = 10.51 KK198 pKa = 10.83 GRR200 pKa = 11.84 GNSLLQGQEE209 pKa = 3.29 ARR211 pKa = 11.84 YY212 pKa = 9.01 AALLALGWARR222 pKa = 11.84 AHH224 pKa = 6.18 LQFSADD230 pKa = 3.61 GQLLEE235 pKa = 4.18 PVIPRR240 pKa = 11.84 EE241 pKa = 4.06 LRR243 pKa = 11.84 LKK245 pKa = 10.48 AVQRR249 pKa = 11.84 WQLVRR254 pKa = 11.84 RR255 pKa = 11.84 TGQPRR260 pKa = 11.84 NVMRR264 pKa = 11.84 LLKK267 pKa = 10.67 GAFTFEE273 pKa = 4.08 NGASYY278 pKa = 10.1 LAWKK282 pKa = 9.51 IRR284 pKa = 11.84 RR285 pKa = 11.84 HH286 pKa = 5.11 TGFDD290 pKa = 3.16 LRR292 pKa = 11.84 LSAFEE297 pKa = 3.93 QRR299 pKa = 11.84 HH300 pKa = 5.63 PLLMLPALLWRR311 pKa = 11.84 ARR313 pKa = 11.84 KK314 pKa = 8.72 LWNRR318 pKa = 11.84 PQDD321 pKa = 3.27 GMVPEE326 pKa = 4.97 KK327 pKa = 11.14 KK328 pKa = 10.33 NGTQQ332 pKa = 3.1
Molecular weight: 37.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.604
IPC_protein 10.628
Toseland 10.701
ProMoST 10.54
Dawson 10.789
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 10.833
Grimsley 10.847
Solomon 10.979
Lehninger 10.935
Nozaki 10.701
DTASelect 10.57
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.54
IPC_peptide 10.979
IPC2_peptide 9.721
IPC2.peptide.svr19 8.666
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2846
0
2846
857605
37
1992
301.3
32.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.011 ± 0.058
1.032 ± 0.016
5.598 ± 0.034
5.324 ± 0.044
3.567 ± 0.03
8.416 ± 0.044
2.467 ± 0.025
4.971 ± 0.035
2.914 ± 0.033
10.464 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.022
2.773 ± 0.034
5.583 ± 0.04
3.583 ± 0.032
7.239 ± 0.054
6.017 ± 0.042
5.816 ± 0.037
7.023 ± 0.042
1.385 ± 0.02
2.237 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here