Haloarcula hispanica pleomorphic virus 3
Average proteome isoelectric point is 4.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C8V5Y3|A0A1C8V5Y3_9VIRU Uncharacterized protein OS=Haloarcula hispanica pleomorphic virus 3 OX=1879051 PE=4 SV=1
MM1 pKa = 7.5 AALAASPTMFGKK13 pKa = 9.89 IDD15 pKa = 3.83 IEE17 pKa = 4.21 DD18 pKa = 3.8 ALAGVIFTASAFVTNGIASISLLGYY43 pKa = 10.45 DD44 pKa = 4.29 LAASVFTVQGTSIDD58 pKa = 3.82 LAFLLSLVALAMAYY72 pKa = 7.68 ATNRR76 pKa = 11.84 VNEE79 pKa = 4.26 SRR81 pKa = 11.84 NKK83 pKa = 10.3 NYY85 pKa = 9.95 QLDD88 pKa = 3.87 TDD90 pKa = 4.62 LVDD93 pKa = 3.32 IAKK96 pKa = 10.58 GSATVEE102 pKa = 4.21 TYY104 pKa = 10.68 LALGTLVIVLFTGLNILGAQDD125 pKa = 3.35 IVVGSAAIGLTVVAVEE141 pKa = 3.67 AAGYY145 pKa = 9.17 YY146 pKa = 9.57 VISYY150 pKa = 10.2 LGG152 pKa = 3.24
Molecular weight: 15.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.999
IPC_protein 3.897
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.024
Patrickios 0.782
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.923
Protein with the highest isoelectric point:
>tr|A0A1C8V5Z2|A0A1C8V5Z2_9VIRU Uncharacterized protein OS=Haloarcula hispanica pleomorphic virus 3 OX=1879051 PE=4 SV=1
MM1 pKa = 7.34 SRR3 pKa = 11.84 SHH5 pKa = 6.95 KK6 pKa = 10.07 ANHH9 pKa = 6.33 YY10 pKa = 6.72 GTLVEE15 pKa = 4.69 RR16 pKa = 11.84 KK17 pKa = 8.94 AAEE20 pKa = 4.35 RR21 pKa = 11.84 YY22 pKa = 10.16 DD23 pKa = 4.4 LDD25 pKa = 4.39 LDD27 pKa = 3.64 RR28 pKa = 11.84 CSWHH32 pKa = 6.96 DD33 pKa = 3.52 AKK35 pKa = 11.02 RR36 pKa = 11.84 PDD38 pKa = 3.58 GTPVEE43 pKa = 4.88 IKK45 pKa = 10.56 SAMHH49 pKa = 6.22 RR50 pKa = 11.84 HH51 pKa = 6.43 ADD53 pKa = 3.76 GQPGTFKK60 pKa = 11.04 LYY62 pKa = 10.68 DD63 pKa = 3.6 RR64 pKa = 11.84 YY65 pKa = 10.45 HH66 pKa = 6.13 RR67 pKa = 11.84 RR68 pKa = 11.84 LRR70 pKa = 11.84 AEE72 pKa = 4.05 NGWYY76 pKa = 10.3 AFGVYY81 pKa = 8.82 RR82 pKa = 11.84 VRR84 pKa = 11.84 GRR86 pKa = 11.84 GIEE89 pKa = 3.94 VLEE92 pKa = 3.82 WEE94 pKa = 4.51 IRR96 pKa = 11.84 HH97 pKa = 6.05 SSRR100 pKa = 11.84 LPKK103 pKa = 10.15 LDD105 pKa = 2.95 WHH107 pKa = 6.83 GGGIHH112 pKa = 7.4 RR113 pKa = 11.84 DD114 pKa = 3.22 AKK116 pKa = 8.96 QAKK119 pKa = 9.19 IPVSEE124 pKa = 4.2 LRR126 pKa = 11.84 YY127 pKa = 10.11
Molecular weight: 14.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 9.297
IPC_protein 9.502
Toseland 9.838
ProMoST 9.677
Dawson 10.101
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.321
Grimsley 10.204
Solomon 10.145
Lehninger 10.101
Nozaki 9.838
DTASelect 9.823
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.014
Patrickios 7.614
IPC_peptide 10.145
IPC2_peptide 8.565
IPC2.peptide.svr19 8.33
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
3367
47
613
198.1
22.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.554 ± 0.698
0.743 ± 0.179
7.692 ± 0.45
8.108 ± 0.698
2.465 ± 0.287
7.722 ± 0.469
2.138 ± 0.36
5.227 ± 0.576
4.039 ± 0.352
7.069 ± 0.432
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.782 ± 0.16
3.713 ± 0.55
4.544 ± 0.423
4.514 ± 0.504
6.207 ± 0.888
6.118 ± 0.711
6.801 ± 0.706
6.742 ± 0.438
1.544 ± 0.27
4.277 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here