Pelagirhabdus alkalitolerans
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2406 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6GQV9|A0A1G6GQV9_9BACI Histidine kinase OS=Pelagirhabdus alkalitolerans OX=1612202 GN=SAMN05421734_101408 PE=4 SV=1
MM1 pKa = 6.69 MKK3 pKa = 10.17 KK4 pKa = 9.77 YY5 pKa = 11.0 VKK7 pKa = 10.48 GFLFAVLITLVLAGCGNGDD26 pKa = 4.12 EE27 pKa = 4.92 NGNDD31 pKa = 3.29 QAGDD35 pKa = 3.83 SEE37 pKa = 4.75 AEE39 pKa = 4.12 AVEE42 pKa = 4.15 LTLFSSVTNEE52 pKa = 3.2 SDD54 pKa = 3.2 QAIFEE59 pKa = 4.27 EE60 pKa = 4.73 VIEE63 pKa = 4.31 RR64 pKa = 11.84 FEE66 pKa = 4.25 EE67 pKa = 4.37 EE68 pKa = 3.99 NDD70 pKa = 4.37 HH71 pKa = 7.14 IQIDD75 pKa = 4.37 YY76 pKa = 10.77 NFPTSEE82 pKa = 4.43 YY83 pKa = 8.69 EE84 pKa = 3.75 SMMRR88 pKa = 11.84 VRR90 pKa = 11.84 MGADD94 pKa = 3.33 DD95 pKa = 4.34 MPDD98 pKa = 3.16 LFDD101 pKa = 3.36 THH103 pKa = 5.67 GWAVNRR109 pKa = 11.84 YY110 pKa = 8.83 GDD112 pKa = 3.62 YY113 pKa = 11.34 TMDD116 pKa = 4.17 LRR118 pKa = 11.84 DD119 pKa = 4.15 MDD121 pKa = 4.0 WVDD124 pKa = 4.07 NLDD127 pKa = 4.0 PSLEE131 pKa = 4.34 PIITDD136 pKa = 4.06 DD137 pKa = 3.95 DD138 pKa = 4.69 GKK140 pKa = 11.41 VYY142 pKa = 10.32 AYY144 pKa = 10.06 PMNQANDD151 pKa = 3.55 GLTYY155 pKa = 10.76 NVGILEE161 pKa = 4.74 EE162 pKa = 4.16 YY163 pKa = 10.63 DD164 pKa = 2.97 IDD166 pKa = 4.01 VPEE169 pKa = 4.59 TFDD172 pKa = 3.78 EE173 pKa = 4.93 LMDD176 pKa = 4.26 ALRR179 pKa = 11.84 EE180 pKa = 4.09 IRR182 pKa = 11.84 DD183 pKa = 3.52 QSDD186 pKa = 4.08 GEE188 pKa = 4.44 VTPLWFPGSDD198 pKa = 3.37 RR199 pKa = 11.84 YY200 pKa = 11.12 SLGYY204 pKa = 10.21 FLDD207 pKa = 3.68 IMSTPLLVTHH217 pKa = 6.97 EE218 pKa = 4.1 DD219 pKa = 3.06 HH220 pKa = 8.04 DD221 pKa = 4.58 YY222 pKa = 11.54 SEE224 pKa = 4.26 EE225 pKa = 4.07 LKK227 pKa = 10.9 AGEE230 pKa = 4.44 YY231 pKa = 10.73 GWDD234 pKa = 3.41 EE235 pKa = 4.0 YY236 pKa = 11.42 TYY238 pKa = 11.07 LPEE241 pKa = 6.2 KK242 pKa = 9.57 LLQMQEE248 pKa = 3.92 EE249 pKa = 4.49 DD250 pKa = 4.03 LLNVDD255 pKa = 3.77 VLTAQTHH262 pKa = 5.11 EE263 pKa = 4.18 QTQLFAQDD271 pKa = 3.32 KK272 pKa = 9.43 IGFVMGTLPVDD283 pKa = 4.65 SIHH286 pKa = 6.61 EE287 pKa = 4.16 LNEE290 pKa = 4.74 DD291 pKa = 3.44 IEE293 pKa = 4.69 LGIMPVPPIHH303 pKa = 7.81 DD304 pKa = 4.15 GGEE307 pKa = 4.01 KK308 pKa = 9.95 SWIGGEE314 pKa = 4.05 RR315 pKa = 11.84 FTLAVAQNSDD325 pKa = 3.36 HH326 pKa = 7.05 PEE328 pKa = 3.63 EE329 pKa = 4.26 AKK331 pKa = 10.71 QFIEE335 pKa = 5.72 FLAQPDD341 pKa = 3.54 IAQKK345 pKa = 10.35 VAEE348 pKa = 4.46 GTNLPSGLEE357 pKa = 3.97 NVSAEE362 pKa = 3.78 IYY364 pKa = 9.87 YY365 pKa = 9.84 QDD367 pKa = 4.19 FYY369 pKa = 11.88 EE370 pKa = 5.0 EE371 pKa = 4.25 YY372 pKa = 10.42 DD373 pKa = 3.77 DD374 pKa = 6.16 VIVEE378 pKa = 4.29 PYY380 pKa = 9.85 FDD382 pKa = 5.48 RR383 pKa = 11.84 EE384 pKa = 4.07 FLPSGMWDD392 pKa = 3.4 VYY394 pKa = 11.55 GEE396 pKa = 4.21 TAQEE400 pKa = 3.97 LLSGRR405 pKa = 11.84 MTPEE409 pKa = 3.4 EE410 pKa = 4.16 VTEE413 pKa = 4.24 QMAAEE418 pKa = 4.1 YY419 pKa = 10.62 NRR421 pKa = 11.84 LYY423 pKa = 10.98 DD424 pKa = 3.78 EE425 pKa = 4.47 EE426 pKa = 4.33
Molecular weight: 48.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.579
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.897
Patrickios 1.418
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A1G6LCH0|A0A1G6LCH0_9BACI Uncharacterized protein OS=Pelagirhabdus alkalitolerans OX=1612202 GN=SAMN05421734_10835 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 8.86 HH12 pKa = 6.39 KK13 pKa = 9.52 KK14 pKa = 7.68 VHH16 pKa = 5.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 10.35 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.36 KK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.66 VLSAA44 pKa = 4.05
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2406
0
2406
744324
31
2683
309.4
34.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.419 ± 0.048
0.55 ± 0.012
6.432 ± 0.054
7.779 ± 0.066
4.375 ± 0.042
6.273 ± 0.049
2.359 ± 0.029
7.811 ± 0.05
6.088 ± 0.06
9.662 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.025
4.411 ± 0.034
3.397 ± 0.028
4.295 ± 0.032
4.02 ± 0.032
6.155 ± 0.035
5.659 ± 0.034
7.024 ± 0.039
0.888 ± 0.017
3.656 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here