Microbacterium saccharophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8HTV3|A0A5C8HTV3_9MICO DUF3093 domain-containing protein OS=Microbacterium saccharophilum OX=1213358 GN=FVP74_11305 PE=4 SV=1
MM1 pKa = 7.46NPRR4 pKa = 11.84RR5 pKa = 11.84LLAGSALGIVALLSLTACATPSSSSGSPDD34 pKa = 2.92APAGHH39 pKa = 6.61SLGSLWPAPPEE50 pKa = 4.17SEE52 pKa = 4.48VVGQGTVMDD61 pKa = 3.72VGGAPEE67 pKa = 4.65LCLGPIMEE75 pKa = 5.23SYY77 pKa = 10.51PPQCHH82 pKa = 6.89GIPLAGWSWDD92 pKa = 3.48GVDD95 pKa = 3.67GAEE98 pKa = 4.55SVGDD102 pKa = 4.0VRR104 pKa = 11.84WGTYY108 pKa = 9.22AVQGTYY114 pKa = 10.4DD115 pKa = 3.95GEE117 pKa = 4.61TLTVTRR123 pKa = 11.84PPIMLALYY131 pKa = 10.04DD132 pKa = 3.86PMMTEE137 pKa = 4.9DD138 pKa = 3.7PTGGKK143 pKa = 9.99AGAGEE148 pKa = 3.97EE149 pKa = 4.71AEE151 pKa = 4.13LQEE154 pKa = 4.71IQDD157 pKa = 4.55ALPDD161 pKa = 3.97LLGAEE166 pKa = 4.43YY167 pKa = 10.64LSSFPEE173 pKa = 4.22NGWLWVDD180 pKa = 3.79VVWDD184 pKa = 4.0DD185 pKa = 4.63GTWQKK190 pKa = 11.29AADD193 pKa = 4.2DD194 pKa = 4.58DD195 pKa = 4.69YY196 pKa = 10.71GTGVVVIGSALRR208 pKa = 11.84AVSGG212 pKa = 3.67

Molecular weight: 21.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8I1D3|A0A5C8I1D3_9MICO Recombinase family protein OS=Microbacterium saccharophilum OX=1213358 GN=FVP74_09325 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2750

0

2750

902738

32

2021

328.3

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.196 ± 0.074

0.5 ± 0.01

6.41 ± 0.041

5.537 ± 0.042

3.056 ± 0.025

9.033 ± 0.038

1.992 ± 0.022

4.418 ± 0.034

1.818 ± 0.032

10.156 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.746 ± 0.018

1.759 ± 0.022

5.531 ± 0.031

2.673 ± 0.023

7.556 ± 0.05

5.085 ± 0.03

6.036 ± 0.042

9.017 ± 0.042

1.524 ± 0.019

1.956 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski