Amino acid dipepetide frequency for Microbacterium saccharophilum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.862AlaAla: 22.862 ± 0.301	0.749AlaCys: 0.749 ± 0.034	9.291AlaAsp: 9.291 ± 0.122	8.398AlaGlu: 8.398 ± 0.132	4.086AlaPhe: 4.086 ± 0.07	13.077AlaGly: 13.077 ± 0.144	2.661AlaHis: 2.661 ± 0.06	5.594AlaIle: 5.594 ± 0.082	2.553AlaLys: 2.553 ± 0.073	14.917AlaLeu: 14.917 ± 0.168	2.717AlaMet: 2.717 ± 0.056	2.058AlaAsn: 2.058 ± 0.048	7.194AlaPro: 7.194 ± 0.126	3.924AlaGln: 3.924 ± 0.082	10.244AlaArg: 10.244 ± 0.152	6.83AlaSer: 6.83 ± 0.094	7.672AlaThr: 7.672 ± 0.105	12.516AlaVal: 12.516 ± 0.14	2.077AlaTrp: 2.077 ± 0.053	2.544AlaTyr: 2.544 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.727CysAla: 0.727 ± 0.029	0.051CysCys: 0.051 ± 0.009	0.335CysAsp: 0.335 ± 0.021	0.234CysGlu: 0.234 ± 0.019	0.161CysPhe: 0.161 ± 0.014	0.561CysGly: 0.561 ± 0.026	0.113CysHis: 0.113 ± 0.011	0.177CysIle: 0.177 ± 0.014	0.052CysLys: 0.052 ± 0.008	0.457CysLeu: 0.457 ± 0.026	0.071CysMet: 0.071 ± 0.01	0.097CysAsn: 0.097 ± 0.011	0.294CysPro: 0.294 ± 0.021	0.124CysGln: 0.124 ± 0.012	0.381CysArg: 0.381 ± 0.021	0.268CysSer: 0.268 ± 0.019	0.311CysThr: 0.311 ± 0.02	0.43CysVal: 0.43 ± 0.022	0.068CysTrp: 0.068 ± 0.009	0.092CysTyr: 0.092 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	10.175AspAla: 10.175 ± 0.128	0.225AspCys: 0.225 ± 0.015	4.401AspAsp: 4.401 ± 0.085	4.349AspGlu: 4.349 ± 0.077	1.673AspPhe: 1.673 ± 0.044	6.613AspGly: 6.613 ± 0.101	1.173AspHis: 1.173 ± 0.037	2.532AspIle: 2.532 ± 0.05	0.955AspLys: 0.955 ± 0.041	6.612AspLeu: 6.612 ± 0.085	0.861AspMet: 0.861 ± 0.026	0.927AspAsn: 0.927 ± 0.037	4.378AspPro: 4.378 ± 0.085	1.438AspGln: 1.438 ± 0.041	4.681AspArg: 4.681 ± 0.084	2.222AspSer: 2.222 ± 0.049	2.972AspThr: 2.972 ± 0.064	5.784AspVal: 5.784 ± 0.083	0.923AspTrp: 0.923 ± 0.036	1.206AspTyr: 1.206 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	6.956GluAla: 6.956 ± 0.115	0.27GluCys: 0.27 ± 0.019	3.053GluAsp: 3.053 ± 0.061	3.07GluGlu: 3.07 ± 0.076	1.666GluPhe: 1.666 ± 0.042	3.982GluGly: 3.982 ± 0.071	1.366GluHis: 1.366 ± 0.039	2.803GluIle: 2.803 ± 0.053	1.318GluLys: 1.318 ± 0.043	6.285GluLeu: 6.285 ± 0.085	0.895GluMet: 0.895 ± 0.034	1.181GluAsn: 1.181 ± 0.04	3.023GluPro: 3.023 ± 0.069	1.996GluGln: 1.996 ± 0.054	5.106GluArg: 5.106 ± 0.097	2.657GluSer: 2.657 ± 0.063	3.053GluThr: 3.053 ± 0.049	4.646GluVal: 4.646 ± 0.08	0.897GluTrp: 0.897 ± 0.033	1.149GluTyr: 1.149 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.507PheAla: 4.507 ± 0.08	0.14PheCys: 0.14 ± 0.013	2.316PheAsp: 2.316 ± 0.054	1.643PheGlu: 1.643 ± 0.046	1.045PhePhe: 1.045 ± 0.041	3.315PheGly: 3.315 ± 0.072	0.558PheHis: 0.558 ± 0.025	1.119PheIle: 1.119 ± 0.04	0.422PheLys: 0.422 ± 0.024	2.955PheLeu: 2.955 ± 0.068	0.407PheMet: 0.407 ± 0.024	0.577PheAsn: 0.577 ± 0.029	1.404PhePro: 1.404 ± 0.043	0.751PheGln: 0.751 ± 0.03	1.851PheArg: 1.851 ± 0.046	1.616PheSer: 1.616 ± 0.048	2.074PheThr: 2.074 ± 0.048	2.702PheVal: 2.702 ± 0.058	0.495PheTrp: 0.495 ± 0.024	0.665PheTyr: 0.665 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.488GlyAla: 11.488 ± 0.142	0.562GlyCys: 0.562 ± 0.025	5.54GlyAsp: 5.54 ± 0.077	5.15GlyGlu: 5.15 ± 0.07	3.23GlyPhe: 3.23 ± 0.064	7.982GlyGly: 7.982 ± 0.102	1.87GlyHis: 1.87 ± 0.045	4.818GlyIle: 4.818 ± 0.076	2.039GlyLys: 2.039 ± 0.058	8.542GlyLeu: 8.542 ± 0.127	2.054GlyMet: 2.054 ± 0.052	1.536GlyAsn: 1.536 ± 0.045	3.965GlyPro: 3.965 ± 0.078	2.433GlyGln: 2.433 ± 0.058	6.573GlyArg: 6.573 ± 0.086	4.857GlySer: 4.857 ± 0.086	5.604GlyThr: 5.604 ± 0.096	8.223GlyVal: 8.223 ± 0.111	1.655GlyTrp: 1.655 ± 0.048	2.213GlyTyr: 2.213 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	2.602HisAla: 2.602 ± 0.064	0.117HisCys: 0.117 ± 0.01	1.291HisAsp: 1.291 ± 0.041	1.106HisGlu: 1.106 ± 0.041	0.531HisPhe: 0.531 ± 0.023	2.034HisGly: 2.034 ± 0.052	0.575HisHis: 0.575 ± 0.029	0.79HisIle: 0.79 ± 0.03	0.261HisLys: 0.261 ± 0.019	2.002HisLeu: 2.002 ± 0.048	0.318HisMet: 0.318 ± 0.02	0.342HisAsn: 0.342 ± 0.018	1.549HisPro: 1.549 ± 0.05	0.424HisGln: 0.424 ± 0.022	1.639HisArg: 1.639 ± 0.051	0.898HisSer: 0.898 ± 0.033	1.02HisThr: 1.02 ± 0.034	1.742HisVal: 1.742 ± 0.044	0.28HisTrp: 0.28 ± 0.015	0.4HisTyr: 0.4 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.057IleAla: 7.057 ± 0.097	0.233IleCys: 0.233 ± 0.018	3.371IleAsp: 3.371 ± 0.059	2.681IleGlu: 2.681 ± 0.061	1.077IlePhe: 1.077 ± 0.042	4.526IleGly: 4.526 ± 0.077	0.736IleHis: 0.736 ± 0.027	1.697IleIle: 1.697 ± 0.05	0.691IleLys: 0.691 ± 0.031	3.857IleLeu: 3.857 ± 0.072	0.588IleMet: 0.588 ± 0.028	0.774IleAsn: 0.774 ± 0.033	2.48IlePro: 2.48 ± 0.056	0.949IleGln: 0.949 ± 0.033	2.926IleArg: 2.926 ± 0.05	2.045IleSer: 2.045 ± 0.043	2.659IleThr: 2.659 ± 0.063	4.659IleVal: 4.659 ± 0.09	0.485IleTrp: 0.485 ± 0.021	0.689IleTyr: 0.689 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	2.309LysAla: 2.309 ± 0.068	0.064LysCys: 0.064 ± 0.008	1.076LysAsp: 1.076 ± 0.043	0.846LysGlu: 0.846 ± 0.04	0.39LysPhe: 0.39 ± 0.021	1.462LysGly: 1.462 ± 0.044	0.38LysHis: 0.38 ± 0.022	0.881LysIle: 0.881 ± 0.037	0.699LysLys: 0.699 ± 0.04	1.768LysLeu: 1.768 ± 0.051	0.333LysMet: 0.333 ± 0.018	0.434LysAsn: 0.434 ± 0.021	1.12LysPro: 1.12 ± 0.044	0.603LysGln: 0.603 ± 0.031	1.415LysArg: 1.415 ± 0.048	1.005LysSer: 1.005 ± 0.036	1.257LysThr: 1.257 ± 0.038	1.554LysVal: 1.554 ± 0.056	0.202LysTrp: 0.202 ± 0.015	0.385LysTyr: 0.385 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	15.144LeuAla: 15.144 ± 0.172	0.51LeuCys: 0.51 ± 0.022	6.785LeuAsp: 6.785 ± 0.089	5.221LeuGlu: 5.221 ± 0.094	2.88LeuPhe: 2.88 ± 0.071	9.029LeuGly: 9.029 ± 0.121	1.826LeuHis: 1.826 ± 0.048	4.404LeuIle: 4.404 ± 0.084	1.605LeuLys: 1.605 ± 0.051	10.444LeuLeu: 10.444 ± 0.149	1.463LeuMet: 1.463 ± 0.04	1.654LeuAsn: 1.654 ± 0.047	5.628LeuPro: 5.628 ± 0.084	2.58LeuGln: 2.58 ± 0.051	8.017LeuArg: 8.017 ± 0.116	5.513LeuSer: 5.513 ± 0.082	6.495LeuThr: 6.495 ± 0.089	9.298LeuVal: 9.298 ± 0.115	1.404LeuTrp: 1.404 ± 0.041	1.658LeuTyr: 1.658 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.109MetAla: 2.109 ± 0.052	0.102MetCys: 0.102 ± 0.012	0.824MetAsp: 0.824 ± 0.032	0.578MetGlu: 0.578 ± 0.026	0.486MetPhe: 0.486 ± 0.024	1.338MetGly: 1.338 ± 0.039	0.28MetHis: 0.28 ± 0.016	0.806MetIle: 0.806 ± 0.027	0.373MetLys: 0.373 ± 0.02	1.798MetLeu: 1.798 ± 0.048	0.34MetMet: 0.34 ± 0.02	0.479MetAsn: 0.479 ± 0.024	1.183MetPro: 1.183 ± 0.038	0.515MetGln: 0.515 ± 0.024	1.417MetArg: 1.417 ± 0.036	1.38MetSer: 1.38 ± 0.04	1.719MetThr: 1.719 ± 0.04	1.241MetVal: 1.241 ± 0.042	0.206MetTrp: 0.206 ± 0.016	0.286MetTyr: 0.286 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.323AsnAla: 2.323 ± 0.052	0.097AsnCys: 0.097 ± 0.011	1.049AsnAsp: 1.049 ± 0.035	0.881AsnGlu: 0.881 ± 0.035	0.548AsnPhe: 0.548 ± 0.027	1.624AsnGly: 1.624 ± 0.05	0.318AsnHis: 0.318 ± 0.019	0.719AsnIle: 0.719 ± 0.027	0.34AsnLys: 0.34 ± 0.022	1.837AsnLeu: 1.837 ± 0.05	0.285AsnMet: 0.285 ± 0.018	0.422AsnAsn: 0.422 ± 0.025	1.442AsnPro: 1.442 ± 0.047	0.457AsnGln: 0.457 ± 0.024	1.185AsnArg: 1.185 ± 0.037	0.791AsnSer: 0.791 ± 0.035	0.95AsnThr: 0.95 ± 0.032	1.607AsnVal: 1.607 ± 0.043	0.27AsnTrp: 0.27 ± 0.018	0.445AsnTyr: 0.445 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	8.269ProAla: 8.269 ± 0.121	0.191ProCys: 0.191 ± 0.017	4.094ProAsp: 4.094 ± 0.077	3.798ProGlu: 3.798 ± 0.07	1.677ProPhe: 1.677 ± 0.042	5.186ProGly: 5.186 ± 0.099	1.19ProHis: 1.19 ± 0.044	2.088ProIle: 2.088 ± 0.044	0.885ProLys: 0.885 ± 0.035	5.157ProLeu: 5.157 ± 0.076	0.857ProMet: 0.857 ± 0.032	0.906ProAsn: 0.906 ± 0.034	2.556ProPro: 2.556 ± 0.078	1.562ProGln: 1.562 ± 0.042	3.719ProArg: 3.719 ± 0.066	2.875ProSer: 2.875 ± 0.058	3.307ProThr: 3.307 ± 0.066	5.039ProVal: 5.039 ± 0.076	0.916ProTrp: 0.916 ± 0.033	1.041ProTyr: 1.041 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.457GlnAla: 3.457 ± 0.068	0.101GlnCys: 0.101 ± 0.012	1.278GlnAsp: 1.278 ± 0.038	1.322GlnGlu: 1.322 ± 0.044	0.808GlnPhe: 0.808 ± 0.034	1.98GlnGly: 1.98 ± 0.048	0.572GlnHis: 0.572 ± 0.027	1.268GlnIle: 1.268 ± 0.038	0.626GlnLys: 0.626 ± 0.028	2.984GlnLeu: 2.984 ± 0.069	0.49GlnMet: 0.49 ± 0.026	0.594GlnAsn: 0.594 ± 0.026	1.488GlnPro: 1.488 ± 0.041	1.058GlnGln: 1.058 ± 0.034	2.482GlnArg: 2.482 ± 0.053	1.355GlnSer: 1.355 ± 0.04	1.54GlnThr: 1.54 ± 0.038	2.27GlnVal: 2.27 ± 0.053	0.452GlnTrp: 0.452 ± 0.021	0.606GlnTyr: 0.606 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	9.967ArgAla: 9.967 ± 0.136	0.347ArgCys: 0.347 ± 0.023	4.657ArgAsp: 4.657 ± 0.087	4.302ArgGlu: 4.302 ± 0.079	2.418ArgPhe: 2.418 ± 0.055	5.969ArgGly: 5.969 ± 0.089	1.724ArgHis: 1.724 ± 0.046	3.772ArgIle: 3.772 ± 0.065	1.282ArgLys: 1.282 ± 0.039	7.677ArgLeu: 7.677 ± 0.118	1.724ArgMet: 1.724 ± 0.041	1.22ArgAsn: 1.22 ± 0.036	3.959ArgPro: 3.959 ± 0.084	1.957ArgGln: 1.957 ± 0.045	7.116ArgArg: 7.116 ± 0.132	3.87ArgSer: 3.87 ± 0.079	4.522ArgThr: 4.522 ± 0.073	6.355ArgVal: 6.355 ± 0.093	1.176ArgTrp: 1.176 ± 0.036	1.544ArgTyr: 1.544 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.888SerAla: 6.888 ± 0.099	0.249SerCys: 0.249 ± 0.016	2.886SerAsp: 2.886 ± 0.06	2.374SerGlu: 2.374 ± 0.052	1.782SerPhe: 1.782 ± 0.044	5.46SerGly: 5.46 ± 0.087	0.971SerHis: 0.971 ± 0.031	2.249SerIle: 2.249 ± 0.051	0.895SerLys: 0.895 ± 0.033	5.03SerLeu: 5.03 ± 0.084	1.076SerMet: 1.076 ± 0.037	0.891SerAsn: 0.891 ± 0.033	2.964SerPro: 2.964 ± 0.059	1.183SerGln: 1.183 ± 0.035	3.549SerArg: 3.549 ± 0.078	2.847SerSer: 2.847 ± 0.064	3.176SerThr: 3.176 ± 0.065	4.39SerVal: 4.39 ± 0.068	0.895SerTrp: 0.895 ± 0.029	1.094SerTyr: 1.094 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	8.437ThrAla: 8.437 ± 0.106	0.285ThrCys: 0.285 ± 0.02	3.564ThrAsp: 3.564 ± 0.068	2.767ThrGlu: 2.767 ± 0.061	1.884ThrPhe: 1.884 ± 0.053	5.96ThrGly: 5.96 ± 0.095	1.17ThrHis: 1.17 ± 0.041	2.821ThrIle: 2.821 ± 0.057	1.039ThrLys: 1.039 ± 0.041	6.099ThrLeu: 6.099 ± 0.094	0.944ThrMet: 0.944 ± 0.033	1.055ThrAsn: 1.055 ± 0.036	4.067ThrPro: 4.067 ± 0.079	1.456ThrGln: 1.456 ± 0.038	3.877ThrArg: 3.877 ± 0.064	3.22ThrSer: 3.22 ± 0.061	3.729ThrThr: 3.729 ± 0.078	5.869ThrVal: 5.869 ± 0.101	0.918ThrTrp: 0.918 ± 0.031	1.196ThrTyr: 1.196 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	12.477ValAla: 12.477 ± 0.128	0.505ValCys: 0.505 ± 0.024	6.109ValAsp: 6.109 ± 0.085	4.842ValGlu: 4.842 ± 0.083	2.954ValPhe: 2.954 ± 0.075	7.168ValGly: 7.168 ± 0.113	1.772ValHis: 1.772 ± 0.049	4.246ValIle: 4.246 ± 0.084	1.54ValLys: 1.54 ± 0.05	9.253ValLeu: 9.253 ± 0.122	1.397ValMet: 1.397 ± 0.041	1.622ValAsn: 1.622 ± 0.049	4.836ValPro: 4.836 ± 0.086	2.275ValGln: 2.275 ± 0.048	6.416ValArg: 6.416 ± 0.094	4.737ValSer: 4.737 ± 0.078	6.136ValThr: 6.136 ± 0.094	9.034ValVal: 9.034 ± 0.12	1.237ValTrp: 1.237 ± 0.04	1.617ValTyr: 1.617 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	1.665TrpAla: 1.665 ± 0.047	0.101TrpCys: 0.101 ± 0.01	0.832TrpAsp: 0.832 ± 0.032	0.697TrpGlu: 0.697 ± 0.027	0.58TrpPhe: 0.58 ± 0.027	1.153TrpGly: 1.153 ± 0.039	0.364TrpHis: 0.364 ± 0.02	0.688TrpIle: 0.688 ± 0.032	0.275TrpLys: 0.275 ± 0.017	1.786TrpLeu: 1.786 ± 0.045	0.39TrpMet: 0.39 ± 0.021	0.381TrpAsn: 0.381 ± 0.02	0.758TrpPro: 0.758 ± 0.03	0.525TrpGln: 0.525 ± 0.024	1.287TrpArg: 1.287 ± 0.045	0.894TrpSer: 0.894 ± 0.031	0.966TrpThr: 0.966 ± 0.034	1.234TrpVal: 1.234 ± 0.036	0.373TrpTrp: 0.373 ± 0.023	0.287TrpTyr: 0.287 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.547TyrAla: 2.547 ± 0.056	0.105TyrCys: 0.105 ± 0.011	1.349TyrAsp: 1.349 ± 0.044	1.114TyrGlu: 1.114 ± 0.039	0.685TyrPhe: 0.685 ± 0.029	1.914TyrGly: 1.914 ± 0.054	0.304TyrHis: 0.304 ± 0.02	0.708TyrIle: 0.708 ± 0.027	0.331TyrLys: 0.331 ± 0.018	2.098TyrLeu: 2.098 ± 0.049	0.251TyrMet: 0.251 ± 0.017	0.442TyrAsn: 0.442 ± 0.025	1.028TyrPro: 1.028 ± 0.039	0.518TyrGln: 0.518 ± 0.025	1.676TyrArg: 1.676 ± 0.044	0.968TyrSer: 0.968 ± 0.033	1.194TyrThr: 1.194 ± 0.039	1.581TyrVal: 1.581 ± 0.042	0.306TyrTrp: 0.306 ± 0.02	0.436TyrTyr: 0.436 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2750 proteins (902738 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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