Escherichia virus ECG4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TAW5|A0A5J6TAW5_9CAUD Host RNAP inhibitor OS=Escherichia virus ECG4 OX=2601676 GN=ECG4_13 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.13AFIEE21 pKa = 4.8RR22 pKa = 11.84IQYY25 pKa = 10.64DD26 pKa = 4.39HH27 pKa = 6.98LTQDD31 pKa = 4.22DD32 pKa = 4.81DD33 pKa = 4.96YY34 pKa = 11.8SDD36 pKa = 4.23ALHH39 pKa = 6.22EE40 pKa = 4.41VVGGQVRR47 pKa = 11.84TITTRR52 pKa = 11.84FSRR55 pKa = 11.84AADD58 pKa = 4.69GIDD61 pKa = 4.33HH62 pKa = 6.55EE63 pKa = 5.23FEE65 pKa = 5.3DD66 pKa = 4.36SGLMPEE72 pKa = 4.55TKK74 pKa = 10.2DD75 pKa = 3.18VTRR78 pKa = 11.84ILQARR83 pKa = 11.84IYY85 pKa = 8.84EE86 pKa = 4.05ALYY89 pKa = 10.81NDD91 pKa = 3.8VSNSLDD97 pKa = 3.62VVWFEE102 pKa = 5.44DD103 pKa = 3.95EE104 pKa = 4.14EE105 pKa = 5.87ADD107 pKa = 5.03EE108 pKa = 4.35EE109 pKa = 4.27DD110 pKa = 4.56EE111 pKa = 4.47YY112 pKa = 11.18WVVDD116 pKa = 4.07AKK118 pKa = 10.28TGAIIVQAVALEE130 pKa = 4.19VATACAKK137 pKa = 10.32VNYY140 pKa = 9.74AAGRR144 pKa = 11.84HH145 pKa = 5.33LKK147 pKa = 10.6VEE149 pKa = 4.94DD150 pKa = 3.52INDD153 pKa = 3.43NVVFDD158 pKa = 4.99PAAEE162 pKa = 4.18DD163 pKa = 4.0CEE165 pKa = 4.3RR166 pKa = 5.01

Molecular weight: 18.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TE58|A0A5J6TE58_9CAUD Uncharacterized protein OS=Escherichia virus ECG4 OX=2601676 GN=ECG4_18 PE=4 SV=1
MM1 pKa = 7.65INLTHH6 pKa = 7.16SIRR9 pKa = 11.84SYY11 pKa = 11.05TMYY14 pKa = 10.71QNTINFEE21 pKa = 4.48RR22 pKa = 11.84IRR24 pKa = 11.84EE25 pKa = 4.04RR26 pKa = 11.84QQTEE30 pKa = 3.8DD31 pKa = 4.15YY32 pKa = 10.2IPKK35 pKa = 9.92GRR37 pKa = 11.84KK38 pKa = 8.63LNKK41 pKa = 7.87TKK43 pKa = 10.66RR44 pKa = 11.84GGGVKK49 pKa = 10.14GAFRR53 pKa = 11.84NAEE56 pKa = 4.27GKK58 pKa = 10.37DD59 pKa = 3.27SMVNQEE65 pKa = 3.5ILRR68 pKa = 11.84RR69 pKa = 11.84SVSHH73 pKa = 6.33EE74 pKa = 3.74PLRR77 pKa = 11.84AA78 pKa = 3.36

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11530

37

1324

268.1

29.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.5 ± 0.412

1.058 ± 0.17

6.366 ± 0.256

6.644 ± 0.427

3.547 ± 0.277

7.346 ± 0.318

1.899 ± 0.2

4.753 ± 0.224

6.158 ± 0.335

8.508 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.268

4.163 ± 0.272

3.408 ± 0.235

3.773 ± 0.4

5.915 ± 0.271

6.652 ± 0.452

6.088 ± 0.303

7.728 ± 0.321

1.57 ± 0.165

3.122 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski