Amino acid dipepetide frequency for Escherichia virus ECG4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.541AlaAla: 9.541 ± 0.958
0.867AlaCys: 0.867 ± 0.361
5.378AlaAsp: 5.378 ± 0.959
6.245AlaGlu: 6.245 ± 1.26
2.776AlaPhe: 2.776 ± 0.485
7.633AlaGly: 7.633 ± 1.137
0.954AlaHis: 0.954 ± 0.38
5.725AlaIle: 5.725 ± 0.726
6.158AlaLys: 6.158 ± 0.849
7.459AlaLeu: 7.459 ± 1.036
2.255AlaMet: 2.255 ± 0.52
2.949AlaAsn: 2.949 ± 0.639
2.168AlaPro: 2.168 ± 0.523
1.821AlaGln: 1.821 ± 0.547
4.944AlaArg: 4.944 ± 0.661
4.944AlaSer: 4.944 ± 0.817
4.424AlaThr: 4.424 ± 0.785
5.551AlaVal: 5.551 ± 0.898
1.128AlaTrp: 1.128 ± 0.337
2.082AlaTyr: 2.082 ± 0.679
0.0AlaXaa: 0.0 ± 0.0
Cys
0.52CysAla: 0.52 ± 0.196
0.087CysCys: 0.087 ± 0.09
0.694CysAsp: 0.694 ± 0.372
0.607CysGlu: 0.607 ± 0.219
0.434CysPhe: 0.434 ± 0.218
0.607CysGly: 0.607 ± 0.237
0.26CysHis: 0.26 ± 0.144
0.26CysIle: 0.26 ± 0.207
0.434CysLys: 0.434 ± 0.154
1.475CysLeu: 1.475 ± 0.397
0.26CysMet: 0.26 ± 0.191
0.26CysAsn: 0.26 ± 0.172
0.867CysPro: 0.867 ± 0.251
0.173CysGln: 0.173 ± 0.119
0.694CysArg: 0.694 ± 0.648
0.607CysSer: 0.607 ± 0.268
0.26CysThr: 0.26 ± 0.119
1.735CysVal: 1.735 ± 0.695
0.0CysTrp: 0.0 ± 0.0
0.347CysTyr: 0.347 ± 0.244
0.0CysXaa: 0.0 ± 0.0
Asp
6.158AspAla: 6.158 ± 0.73
0.694AspCys: 0.694 ± 0.308
4.337AspAsp: 4.337 ± 0.81
4.51AspGlu: 4.51 ± 0.64
1.908AspPhe: 1.908 ± 0.451
6.766AspGly: 6.766 ± 1.234
1.475AspHis: 1.475 ± 0.475
3.209AspIle: 3.209 ± 0.512
2.515AspLys: 2.515 ± 0.597
5.898AspLeu: 5.898 ± 0.846
1.908AspMet: 1.908 ± 0.505
2.602AspAsn: 2.602 ± 0.582
1.561AspPro: 1.561 ± 0.327
1.475AspGln: 1.475 ± 0.535
2.082AspArg: 2.082 ± 0.572
3.816AspSer: 3.816 ± 0.507
4.163AspThr: 4.163 ± 0.805
4.771AspVal: 4.771 ± 0.905
1.301AspTrp: 1.301 ± 0.439
2.515AspTyr: 2.515 ± 0.643
0.0AspXaa: 0.0 ± 0.0
Glu
6.852GluAla: 6.852 ± 1.089
0.434GluCys: 0.434 ± 0.211
5.378GluAsp: 5.378 ± 0.788
4.857GluGlu: 4.857 ± 0.906
2.602GluPhe: 2.602 ± 0.598
3.73GluGly: 3.73 ± 0.606
0.867GluHis: 0.867 ± 0.349
2.949GluIle: 2.949 ± 0.655
2.862GluLys: 2.862 ± 0.67
5.464GluLeu: 5.464 ± 0.907
1.908GluMet: 1.908 ± 0.498
1.561GluAsn: 1.561 ± 0.465
2.082GluPro: 2.082 ± 0.427
2.949GluGln: 2.949 ± 0.631
4.51GluArg: 4.51 ± 0.875
4.424GluSer: 4.424 ± 1.144
3.296GluThr: 3.296 ± 0.596
5.638GluVal: 5.638 ± 0.874
1.301GluTrp: 1.301 ± 0.395
2.776GluTyr: 2.776 ± 0.534
0.0GluXaa: 0.0 ± 0.0
Phe
2.949PheAla: 2.949 ± 0.587
0.434PheCys: 0.434 ± 0.275
2.602PheAsp: 2.602 ± 0.408
1.648PheGlu: 1.648 ± 0.452
1.301PhePhe: 1.301 ± 0.515
2.342PheGly: 2.342 ± 0.622
0.694PheHis: 0.694 ± 0.282
1.475PheIle: 1.475 ± 0.431
3.036PheLys: 3.036 ± 0.687
3.209PheLeu: 3.209 ± 0.636
0.694PheMet: 0.694 ± 0.39
1.821PheAsn: 1.821 ± 0.526
1.214PhePro: 1.214 ± 0.409
0.434PheGln: 0.434 ± 0.182
1.648PheArg: 1.648 ± 0.451
2.342PheSer: 2.342 ± 0.483
1.735PheThr: 1.735 ± 0.4
3.903PheVal: 3.903 ± 0.755
0.52PheTrp: 0.52 ± 0.205
1.475PheTyr: 1.475 ± 0.446
0.0PheXaa: 0.0 ± 0.0
Gly
4.771GlyAla: 4.771 ± 0.74
1.214GlyCys: 1.214 ± 0.459
5.638GlyAsp: 5.638 ± 0.907
4.857GlyGlu: 4.857 ± 0.969
2.429GlyPhe: 2.429 ± 0.591
5.291GlyGly: 5.291 ± 0.682
1.301GlyHis: 1.301 ± 0.446
2.862GlyIle: 2.862 ± 0.609
4.163GlyLys: 4.163 ± 0.773
6.245GlyLeu: 6.245 ± 1.311
2.168GlyMet: 2.168 ± 0.548
3.036GlyAsn: 3.036 ± 0.877
0.781GlyPro: 0.781 ± 0.373
2.776GlyGln: 2.776 ± 0.747
6.679GlyArg: 6.679 ± 1.028
5.811GlySer: 5.811 ± 0.729
4.337GlyThr: 4.337 ± 1.049
5.031GlyVal: 5.031 ± 1.027
1.041GlyTrp: 1.041 ± 0.434
3.036GlyTyr: 3.036 ± 0.595
0.0GlyXaa: 0.0 ± 0.0
His
1.128HisAla: 1.128 ± 0.369
0.781HisCys: 0.781 ± 0.437
1.214HisAsp: 1.214 ± 0.5
1.214HisGlu: 1.214 ± 0.508
0.347HisPhe: 0.347 ± 0.225
1.301HisGly: 1.301 ± 0.42
0.52HisHis: 0.52 ± 0.348
1.128HisIle: 1.128 ± 0.564
1.214HisLys: 1.214 ± 0.291
2.429HisLeu: 2.429 ± 0.653
0.52HisMet: 0.52 ± 0.207
0.607HisAsn: 0.607 ± 0.224
0.781HisPro: 0.781 ± 0.259
0.781HisGln: 0.781 ± 0.495
1.128HisArg: 1.128 ± 0.455
0.781HisSer: 0.781 ± 0.269
1.214HisThr: 1.214 ± 0.405
1.388HisVal: 1.388 ± 0.36
0.087HisTrp: 0.087 ± 0.082
0.434HisTyr: 0.434 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
3.99IleAla: 3.99 ± 0.815
0.434IleCys: 0.434 ± 0.266
3.036IleAsp: 3.036 ± 0.556
2.429IleGlu: 2.429 ± 0.327
1.475IlePhe: 1.475 ± 0.476
3.296IleGly: 3.296 ± 0.606
0.867IleHis: 0.867 ± 0.559
2.515IleIle: 2.515 ± 0.732
4.944IleLys: 4.944 ± 0.975
3.296IleLeu: 3.296 ± 0.821
1.214IleMet: 1.214 ± 0.332
2.776IleAsn: 2.776 ± 0.528
1.821IlePro: 1.821 ± 0.575
1.561IleGln: 1.561 ± 0.41
2.776IleArg: 2.776 ± 0.608
1.995IleSer: 1.995 ± 0.522
3.383IleThr: 3.383 ± 0.615
3.73IleVal: 3.73 ± 0.563
1.041IleTrp: 1.041 ± 0.447
0.867IleTyr: 0.867 ± 0.278
0.0IleXaa: 0.0 ± 0.0
Lys
7.112LysAla: 7.112 ± 0.893
0.781LysCys: 0.781 ± 0.389
3.556LysAsp: 3.556 ± 0.501
3.816LysGlu: 3.816 ± 0.474
2.082LysPhe: 2.082 ± 0.486
3.903LysGly: 3.903 ± 0.574
1.735LysHis: 1.735 ± 0.592
1.735LysIle: 1.735 ± 0.328
3.73LysLys: 3.73 ± 1.076
4.597LysLeu: 4.597 ± 0.62
1.388LysMet: 1.388 ± 0.392
2.255LysAsn: 2.255 ± 0.688
2.776LysPro: 2.776 ± 0.695
2.689LysGln: 2.689 ± 0.772
4.163LysArg: 4.163 ± 0.65
3.556LysSer: 3.556 ± 0.661
3.816LysThr: 3.816 ± 0.793
4.771LysVal: 4.771 ± 0.858
1.041LysTrp: 1.041 ± 0.367
2.082LysTyr: 2.082 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
6.679LeuAla: 6.679 ± 0.958
0.26LeuCys: 0.26 ± 0.157
4.684LeuAsp: 4.684 ± 0.69
5.898LeuGlu: 5.898 ± 1.097
2.255LeuPhe: 2.255 ± 0.56
4.597LeuGly: 4.597 ± 0.837
0.867LeuHis: 0.867 ± 0.319
2.342LeuIle: 2.342 ± 0.634
6.505LeuLys: 6.505 ± 0.77
6.679LeuLeu: 6.679 ± 1.359
3.556LeuMet: 3.556 ± 0.754
3.816LeuAsn: 3.816 ± 0.789
3.209LeuPro: 3.209 ± 0.476
5.291LeuGln: 5.291 ± 1.027
5.638LeuArg: 5.638 ± 0.685
6.072LeuSer: 6.072 ± 1.03
6.072LeuThr: 6.072 ± 0.878
7.286LeuVal: 7.286 ± 1.769
1.561LeuTrp: 1.561 ± 0.491
1.821LeuTyr: 1.821 ± 0.468
0.0LeuXaa: 0.0 ± 0.0
Met
2.862MetAla: 2.862 ± 0.627
0.434MetCys: 0.434 ± 0.196
1.214MetAsp: 1.214 ± 0.333
2.255MetGlu: 2.255 ± 0.61
1.301MetPhe: 1.301 ± 0.391
1.995MetGly: 1.995 ± 0.53
0.347MetHis: 0.347 ± 0.15
1.648MetIle: 1.648 ± 0.366
0.867MetLys: 0.867 ± 0.262
2.168MetLeu: 2.168 ± 0.401
0.52MetMet: 0.52 ± 0.201
0.867MetAsn: 0.867 ± 0.268
1.301MetPro: 1.301 ± 0.393
0.867MetGln: 0.867 ± 0.423
1.735MetArg: 1.735 ± 0.48
1.475MetSer: 1.475 ± 0.471
1.821MetThr: 1.821 ± 0.419
3.383MetVal: 3.383 ± 0.75
0.173MetTrp: 0.173 ± 0.127
0.781MetTyr: 0.781 ± 0.302
0.0MetXaa: 0.0 ± 0.0
Asn
4.597AsnAla: 4.597 ± 0.854
0.434AsnCys: 0.434 ± 0.228
1.908AsnAsp: 1.908 ± 0.678
2.255AsnGlu: 2.255 ± 0.51
1.214AsnPhe: 1.214 ± 0.287
4.424AsnGly: 4.424 ± 0.921
0.694AsnHis: 0.694 ± 0.227
1.995AsnIle: 1.995 ± 0.457
2.342AsnLys: 2.342 ± 0.667
3.296AsnLeu: 3.296 ± 0.572
1.214AsnMet: 1.214 ± 0.41
1.735AsnAsn: 1.735 ± 0.593
3.296AsnPro: 3.296 ± 0.899
1.301AsnGln: 1.301 ± 0.489
2.342AsnArg: 2.342 ± 0.622
2.862AsnSer: 2.862 ± 0.665
1.648AsnThr: 1.648 ± 0.387
2.602AsnVal: 2.602 ± 0.55
0.087AsnTrp: 0.087 ± 0.086
1.388AsnTyr: 1.388 ± 0.359
0.0AsnXaa: 0.0 ± 0.0
Pro
2.255ProAla: 2.255 ± 0.553
0.694ProCys: 0.694 ± 0.228
1.908ProAsp: 1.908 ± 0.519
3.643ProGlu: 3.643 ± 0.668
1.388ProPhe: 1.388 ± 0.374
1.301ProGly: 1.301 ± 0.318
0.781ProHis: 0.781 ± 0.627
2.082ProIle: 2.082 ± 0.489
2.862ProLys: 2.862 ± 0.564
1.995ProLeu: 1.995 ± 0.476
1.128ProMet: 1.128 ± 0.276
1.735ProAsn: 1.735 ± 0.514
0.434ProPro: 0.434 ± 0.193
1.735ProGln: 1.735 ± 0.387
1.301ProArg: 1.301 ± 0.461
1.995ProSer: 1.995 ± 0.387
2.255ProThr: 2.255 ± 0.444
2.342ProVal: 2.342 ± 0.533
1.041ProTrp: 1.041 ± 0.537
1.214ProTyr: 1.214 ± 0.34
0.0ProXaa: 0.0 ± 0.0
Gln
3.903GlnAla: 3.903 ± 0.579
0.26GlnCys: 0.26 ± 0.166
3.209GlnAsp: 3.209 ± 0.805
2.602GlnGlu: 2.602 ± 0.592
1.908GlnPhe: 1.908 ± 0.346
1.821GlnGly: 1.821 ± 0.567
0.954GlnHis: 0.954 ± 0.407
1.301GlnIle: 1.301 ± 0.457
1.041GlnLys: 1.041 ± 0.31
4.51GlnLeu: 4.51 ± 1.504
0.954GlnMet: 0.954 ± 0.364
2.168GlnAsn: 2.168 ± 0.909
1.128GlnPro: 1.128 ± 0.501
1.214GlnGln: 1.214 ± 0.35
1.648GlnArg: 1.648 ± 0.483
2.776GlnSer: 2.776 ± 0.543
1.908GlnThr: 1.908 ± 0.569
3.036GlnVal: 3.036 ± 0.815
0.26GlnTrp: 0.26 ± 0.151
1.128GlnTyr: 1.128 ± 0.439
0.0GlnXaa: 0.0 ± 0.0
Arg
4.163ArgAla: 4.163 ± 0.884
0.26ArgCys: 0.26 ± 0.146
4.424ArgAsp: 4.424 ± 0.783
3.73ArgGlu: 3.73 ± 0.775
2.082ArgPhe: 2.082 ± 0.456
3.73ArgGly: 3.73 ± 0.439
1.041ArgHis: 1.041 ± 0.463
3.47ArgIle: 3.47 ± 0.766
2.949ArgLys: 2.949 ± 0.744
5.985ArgLeu: 5.985 ± 0.836
0.954ArgMet: 0.954 ± 0.369
2.602ArgAsn: 2.602 ± 0.619
1.908ArgPro: 1.908 ± 0.396
2.429ArgGln: 2.429 ± 0.367
3.816ArgArg: 3.816 ± 0.749
4.337ArgSer: 4.337 ± 0.746
4.684ArgThr: 4.684 ± 0.996
3.123ArgVal: 3.123 ± 0.607
1.821ArgTrp: 1.821 ± 0.475
1.648ArgTyr: 1.648 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
3.643SerAla: 3.643 ± 0.813
1.041SerCys: 1.041 ± 0.285
4.597SerAsp: 4.597 ± 0.656
3.816SerGlu: 3.816 ± 0.674
3.036SerPhe: 3.036 ± 0.884
6.332SerGly: 6.332 ± 0.865
2.255SerHis: 2.255 ± 0.444
3.816SerIle: 3.816 ± 0.647
3.296SerLys: 3.296 ± 0.639
3.556SerLeu: 3.556 ± 0.659
1.388SerMet: 1.388 ± 0.371
2.342SerAsn: 2.342 ± 0.614
2.342SerPro: 2.342 ± 0.42
2.515SerGln: 2.515 ± 0.669
3.383SerArg: 3.383 ± 0.599
4.684SerSer: 4.684 ± 1.107
4.857SerThr: 4.857 ± 0.871
5.118SerVal: 5.118 ± 0.736
1.301SerTrp: 1.301 ± 0.517
3.209SerTyr: 3.209 ± 0.62
0.0SerXaa: 0.0 ± 0.0
Thr
3.643ThrAla: 3.643 ± 0.719
0.347ThrCys: 0.347 ± 0.154
2.949ThrAsp: 2.949 ± 0.518
4.944ThrGlu: 4.944 ± 0.762
1.735ThrPhe: 1.735 ± 0.459
5.204ThrGly: 5.204 ± 0.995
0.954ThrHis: 0.954 ± 0.333
4.51ThrIle: 4.51 ± 1.177
3.556ThrLys: 3.556 ± 0.644
6.158ThrLeu: 6.158 ± 0.737
2.689ThrMet: 2.689 ± 0.879
2.429ThrAsn: 2.429 ± 0.538
2.168ThrPro: 2.168 ± 0.589
1.995ThrGln: 1.995 ± 0.357
3.036ThrArg: 3.036 ± 0.681
4.424ThrSer: 4.424 ± 1.089
4.163ThrThr: 4.163 ± 0.821
4.51ThrVal: 4.51 ± 0.703
0.52ThrTrp: 0.52 ± 0.198
0.954ThrTyr: 0.954 ± 0.248
0.0ThrXaa: 0.0 ± 0.0
Val
5.985ValAla: 5.985 ± 1.253
0.781ValCys: 0.781 ± 0.277
3.903ValAsp: 3.903 ± 0.543
4.944ValGlu: 4.944 ± 0.844
3.036ValPhe: 3.036 ± 0.687
6.158ValGly: 6.158 ± 1.017
1.561ValHis: 1.561 ± 0.64
2.602ValIle: 2.602 ± 0.951
5.898ValLys: 5.898 ± 0.905
6.332ValLeu: 6.332 ± 0.941
2.429ValMet: 2.429 ± 0.548
3.643ValAsn: 3.643 ± 0.86
2.776ValPro: 2.776 ± 0.552
3.903ValGln: 3.903 ± 0.599
4.684ValArg: 4.684 ± 0.659
5.985ValSer: 5.985 ± 0.861
4.337ValThr: 4.337 ± 0.841
5.811ValVal: 5.811 ± 1.085
0.867ValTrp: 0.867 ± 0.423
1.648ValTyr: 1.648 ± 0.54
0.0ValXaa: 0.0 ± 0.0
Trp
0.347TrpAla: 0.347 ± 0.163
0.087TrpCys: 0.087 ± 0.082
1.214TrpAsp: 1.214 ± 0.449
0.954TrpGlu: 0.954 ± 0.436
0.694TrpPhe: 0.694 ± 0.33
0.694TrpGly: 0.694 ± 0.244
0.347TrpHis: 0.347 ± 0.166
0.26TrpIle: 0.26 ± 0.168
1.301TrpLys: 1.301 ± 0.459
1.735TrpLeu: 1.735 ± 0.484
0.087TrpMet: 0.087 ± 0.08
1.388TrpAsn: 1.388 ± 0.464
0.26TrpPro: 0.26 ± 0.192
0.867TrpGln: 0.867 ± 0.482
0.781TrpArg: 0.781 ± 0.348
1.648TrpSer: 1.648 ± 0.599
0.781TrpThr: 0.781 ± 0.239
1.735TrpVal: 1.735 ± 0.701
0.173TrpTrp: 0.173 ± 0.137
0.347TrpTyr: 0.347 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.903TyrAla: 3.903 ± 0.604
0.26TyrCys: 0.26 ± 0.136
1.821TyrAsp: 1.821 ± 0.415
1.214TyrGlu: 1.214 ± 0.475
1.475TyrPhe: 1.475 ± 0.56
2.342TyrGly: 2.342 ± 0.562
0.52TyrHis: 0.52 ± 0.267
1.648TyrIle: 1.648 ± 0.535
1.908TyrLys: 1.908 ± 0.495
2.602TyrLeu: 2.602 ± 0.57
0.694TyrMet: 0.694 ± 0.298
1.041TyrAsn: 1.041 ± 0.319
1.214TyrPro: 1.214 ± 0.435
0.954TyrGln: 0.954 ± 0.411
2.168TyrArg: 2.168 ± 0.608
1.995TyrSer: 1.995 ± 0.443
1.735TyrThr: 1.735 ± 0.381
1.821TyrVal: 1.821 ± 0.561
0.434TyrTrp: 0.434 ± 0.188
1.475TyrTyr: 1.475 ± 0.494
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (11530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski