Theileria annulata
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3790 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4UIL9|Q4UIL9_THEAN Uncharacterized protein OS=Theileria annulata OX=5874 GN=TA16905 PE=4 SV=1
MM1 pKa = 7.29 NLIITLFAILIHH13 pKa = 6.65 VSICLDD19 pKa = 3.47 PVDD22 pKa = 5.53 IEE24 pKa = 4.5 LLQPLPEE31 pKa = 4.22 EE32 pKa = 5.07 GIVSEE37 pKa = 4.44 EE38 pKa = 4.13 VTFHH42 pKa = 6.89 GIHH45 pKa = 5.53 YY46 pKa = 9.47 QIIVPLGQYY55 pKa = 7.65 YY56 pKa = 9.95 FRR58 pKa = 11.84 TISYY62 pKa = 9.66 NNNPIFQEE70 pKa = 4.0 AQRR73 pKa = 11.84 ACLKK77 pKa = 10.56 VVIVTSDD84 pKa = 3.3 HH85 pKa = 5.96 IKK87 pKa = 10.87 YY88 pKa = 9.96 IFLRR92 pKa = 11.84 IITEE96 pKa = 4.16 DD97 pKa = 2.99 GFEE100 pKa = 4.29 IIRR103 pKa = 11.84 RR104 pKa = 11.84 YY105 pKa = 8.87 STFGLFCFLEE115 pKa = 5.05 RR116 pKa = 11.84 ITDD119 pKa = 3.9 CTSSITDD126 pKa = 4.97 LIAIANSWHH135 pKa = 6.53 HH136 pKa = 5.97 SLNNRR141 pKa = 11.84 GFNIPLADD149 pKa = 3.68 VPFEE153 pKa = 4.15 PVGGPDD159 pKa = 4.18 DD160 pKa = 4.34 DD161 pKa = 4.09 QGPGGGGPDD170 pKa = 4.97 DD171 pKa = 6.5 DD172 pKa = 6.97 DD173 pKa = 7.49 DD174 pKa = 6.4 DD175 pKa = 5.01 DD176 pKa = 5.27 HH177 pKa = 7.07 GPPGDD182 pKa = 4.1 VADD185 pKa = 5.21 QPPQPPGDD193 pKa = 4.49 DD194 pKa = 4.24 DD195 pKa = 5.48 ADD197 pKa = 4.04 SDD199 pKa = 3.92 EE200 pKa = 5.4 HH201 pKa = 8.19 SDD203 pKa = 3.52 NGGDD207 pKa = 3.61 NGEE210 pKa = 4.59 GGAPQDD216 pKa = 3.97 PNQIVQDD223 pKa = 4.06 NNLEE227 pKa = 4.5 AGPQDD232 pKa = 5.32 DD233 pKa = 4.2 IPILIEE239 pKa = 4.13 PDD241 pKa = 3.26 HH242 pKa = 7.05 PGGQDD247 pKa = 2.72 AVQQVVEE254 pKa = 5.03 IGPPSNSPYY263 pKa = 10.47 DD264 pKa = 3.59 YY265 pKa = 10.54 PYY267 pKa = 11.27 FNGFDD272 pKa = 3.66 YY273 pKa = 11.29 LGFDD277 pKa = 4.53 PEE279 pKa = 4.28 GLNLGEE285 pKa = 4.9 NEE287 pKa = 4.67 LDD289 pKa = 4.1 NPALNQPILQDD300 pKa = 4.59 DD301 pKa = 4.57 LEE303 pKa = 4.33 QLLGEE308 pKa = 4.59 AVVEE312 pKa = 4.95 DD313 pKa = 5.12 DD314 pKa = 5.05 FDD316 pKa = 6.05 DD317 pKa = 4.78 PFDD320 pKa = 4.4 VNIIDD325 pKa = 4.17 VNQNNQLVPGDD336 pKa = 4.07 SEE338 pKa = 4.84 QPDD341 pKa = 3.71 SEE343 pKa = 4.48 ANEE346 pKa = 3.9 NGFYY350 pKa = 11.04 DD351 pKa = 4.67 FDD353 pKa = 4.16 VEE355 pKa = 4.26 NN356 pKa = 4.08
Molecular weight: 39.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.49
IPC_protein 3.541
Toseland 3.3
ProMoST 3.706
Dawson 3.541
Bjellqvist 3.694
Wikipedia 3.49
Rodwell 3.363
Grimsley 3.21
Solomon 3.528
Lehninger 3.49
Nozaki 3.643
DTASelect 3.923
Thurlkill 3.363
EMBOSS 3.49
Sillero 3.656
Patrickios 0.579
IPC_peptide 3.528
IPC2_peptide 3.63
IPC2.peptide.svr19 3.645
Protein with the highest isoelectric point:
>tr|Q4UCH8|Q4UCH8_THEAN Uncharacterized protein OS=Theileria annulata OX=5874 GN=TA03600 PE=4 SV=1
MM1 pKa = 7.46 FKK3 pKa = 10.63 YY4 pKa = 10.74 LSQRR8 pKa = 11.84 FFSSVYY14 pKa = 10.87 ANLKK18 pKa = 9.78 RR19 pKa = 11.84 IRR21 pKa = 11.84 PEE23 pKa = 3.75 YY24 pKa = 11.12 VDD26 pKa = 5.38 FEE28 pKa = 5.26 TNFPKK33 pKa = 10.82 VKK35 pKa = 9.64 IEE37 pKa = 3.97 RR38 pKa = 11.84 QLLPVPYY45 pKa = 8.45 TQVRR49 pKa = 11.84 ILFFYY54 pKa = 10.67 NEE56 pKa = 3.81 KK57 pKa = 10.54 NLRR60 pKa = 11.84 FLKK63 pKa = 10.64 NLEE66 pKa = 4.25 TPQTLSNCRR75 pKa = 11.84 VVEE78 pKa = 3.84 QLSIRR83 pKa = 11.84 RR84 pKa = 11.84 VKK86 pKa = 10.72 FGGRR90 pKa = 11.84 NNTGRR95 pKa = 11.84 ITTRR99 pKa = 11.84 HH100 pKa = 5.88 RR101 pKa = 11.84 GGGHH105 pKa = 4.21 VQRR108 pKa = 11.84 IRR110 pKa = 11.84 LIDD113 pKa = 3.92 FKK115 pKa = 10.73 RR116 pKa = 11.84 QRR118 pKa = 11.84 KK119 pKa = 9.77 DD120 pKa = 2.67 IFSTVLRR127 pKa = 11.84 IEE129 pKa = 4.14 YY130 pKa = 10.42 DD131 pKa = 3.53 PTRR134 pKa = 11.84 SAHH137 pKa = 5.51 VALIQYY143 pKa = 10.78 DD144 pKa = 4.26 DD145 pKa = 4.31 GVLSYY150 pKa = 9.95 ILCAAGVLPGTRR162 pKa = 11.84 LLSSSNAPLLPGNSLPLRR180 pKa = 11.84 HH181 pKa = 6.43 IPVNSIVHH189 pKa = 5.19 NVEE192 pKa = 3.66 MRR194 pKa = 11.84 PGAGGQIARR203 pKa = 11.84 SGGVYY208 pKa = 9.06 CTVTEE213 pKa = 4.07 KK214 pKa = 11.32 DD215 pKa = 3.13 EE216 pKa = 4.36 TFATLRR222 pKa = 11.84 LSSTEE227 pKa = 3.74 LRR229 pKa = 11.84 KK230 pKa = 9.84 FPLDD234 pKa = 3.1 CWATIGQISNIKK246 pKa = 9.77 HH247 pKa = 5.61 NEE249 pKa = 4.07 RR250 pKa = 11.84 ILKK253 pKa = 9.99 KK254 pKa = 10.61 AGTRR258 pKa = 11.84 RR259 pKa = 11.84 NMGWRR264 pKa = 11.84 PVVRR268 pKa = 11.84 GIAMNANSHH277 pKa = 5.37 PHH279 pKa = 6.39 GGGNDD284 pKa = 3.14 KK285 pKa = 11.22 SGTKK289 pKa = 9.77 RR290 pKa = 11.84 PKK292 pKa = 9.52 CSIWGICKK300 pKa = 10.28 DD301 pKa = 3.66 GYY303 pKa = 8.72 KK304 pKa = 9.58 TRR306 pKa = 11.84 SKK308 pKa = 10.56 HH309 pKa = 5.68 KK310 pKa = 9.14 PLGFIIRR317 pKa = 11.84 RR318 pKa = 11.84 KK319 pKa = 9.51 LCGRR323 pKa = 11.84 LMKK326 pKa = 10.44 KK327 pKa = 10.47 YY328 pKa = 10.52 GITKK332 pKa = 10.22 RR333 pKa = 11.84 PP334 pKa = 3.26
Molecular weight: 38.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.853
IPC_protein 10.672
Toseland 10.862
ProMoST 10.57
Dawson 10.95
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.213
Grimsley 10.994
Solomon 11.082
Lehninger 11.038
Nozaki 10.847
DTASelect 10.657
Thurlkill 10.862
EMBOSS 11.272
Sillero 10.877
Patrickios 10.906
IPC_peptide 11.082
IPC2_peptide 9.706
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3790
0
3790
2024948
38
5808
534.3
61.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.338 ± 0.039
1.648 ± 0.02
5.765 ± 0.032
6.484 ± 0.043
4.962 ± 0.026
4.487 ± 0.035
2.021 ± 0.012
7.118 ± 0.051
8.059 ± 0.035
10.089 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.054 ± 0.015
7.634 ± 0.054
3.716 ± 0.037
3.078 ± 0.028
3.827 ± 0.031
8.631 ± 0.038
6.011 ± 0.045
5.91 ± 0.029
0.814 ± 0.01
4.354 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here