Cyanobium sp. PCC 7001 
Average proteome isoelectric point is 6.73 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2762 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|B5IKG5|B5IKG5_9CYAN Uncharacterized protein OS=Cyanobium sp. PCC 7001 OX=180281 GN=CPCC7001_1821 PE=4 SV=1MM1 pKa = 7.8  IDD3 pKa = 3.29  VDD5 pKa = 5.29  DD6 pKa = 6.33  LIDD9 pKa = 4.39  CGSVSTGTLRR19 pKa = 11.84  SSDD22 pKa = 3.87  LADD25 pKa = 3.9  ALLGEE30 pKa = 4.52  IDD32 pKa = 3.94  RR33 pKa = 11.84  LGLATHH39 pKa = 7.0  APDD42 pKa = 4.33  ALIHH46 pKa = 6.24  AAQALACFSSPTVEE60 pKa = 4.87  DD61 pKa = 4.64  LPDD64 pKa = 3.6  NMAEE68 pKa = 4.32  TAGEE72 pKa = 4.37  TVSDD76 pKa = 4.06  MIDD79 pKa = 3.3  WLNDD83 pKa = 3.36  HH84 pKa = 7.1  APDD87 pKa = 4.18  GWCLQTLEE95 pKa = 4.82  GDD97 pKa = 4.05  GADD100 pKa = 5.14  LLWQPVTLRR109 pKa = 11.84  AQLQAAGSPWVQSEE123 pKa = 3.78  WDD125 pKa = 3.48  KK126 pKa = 11.35  RR127 pKa = 11.84  SQNDD131 pKa = 4.01  AIDD134 pKa = 4.98  DD135 pKa = 4.22  IIEE138 pKa = 4.46  RR139 pKa = 11.84  FDD141 pKa = 4.07  DD142 pKa = 4.45  CPEE145 pKa = 4.02  ALQDD149 pKa = 4.11  FDD151 pKa = 3.72  ISDD154 pKa = 3.6  WVNVAEE160 pKa = 5.22  CYY162 pKa = 9.91  TGTLLRR168 pKa = 11.84  RR169 pKa = 11.84  WEE171 pKa = 4.08  LQEE174 pKa = 3.66  QSIRR178 pKa = 11.84  ALFDD182 pKa = 4.47  DD183 pKa = 4.49  YY184 pKa = 11.34  CDD186 pKa = 3.91  QLGCTSALEE195 pKa = 4.23  ALDD198 pKa = 3.99  GQTIEE203 pKa = 5.67  DD204 pKa = 4.49  PDD206 pKa = 4.78  DD207 pKa = 3.45  FTAAYY212 pKa = 10.25  VNLAMTWGAQCLLRR226 pKa = 11.84  EE227 pKa = 4.55  IDD229 pKa = 3.91  PDD231 pKa = 3.7  HH232 pKa = 6.78  
 25.55 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.751 
IPC2_protein 3.579 
IPC_protein 3.617 
Toseland    3.389 
ProMoST     3.795 
Dawson      3.63 
Bjellqvist  3.783 
Wikipedia   3.579 
Rodwell     3.439 
Grimsley    3.287 
Solomon     3.617 
Lehninger   3.579 
Nozaki      3.732 
DTASelect   4.024 
Thurlkill   3.452 
EMBOSS      3.592 
Sillero     3.745 
Patrickios  1.837 
IPC_peptide 3.605 
IPC2_peptide  3.719 
IPC2.peptide.svr19  3.697 
 Protein with the highest isoelectric point: 
>tr|B5INV1|B5INV1_9CYAN 30S ribosomal protein S4 OS=Cyanobium sp. PCC 7001 OX=180281 GN=rpsD PE=3 SV=1MM1 pKa = 7.56  TKK3 pKa = 9.03  RR4 pKa = 11.84  TLEE7 pKa = 3.92  GTSRR11 pKa = 11.84  KK12 pKa = 9.24  RR13 pKa = 11.84  KK14 pKa = 7.95  RR15 pKa = 11.84  VSGFRR20 pKa = 11.84  VRR22 pKa = 11.84  MRR24 pKa = 11.84  THH26 pKa = 5.95  TGRR29 pKa = 11.84  RR30 pKa = 11.84  VIRR33 pKa = 11.84  SRR35 pKa = 11.84  RR36 pKa = 11.84  RR37 pKa = 11.84  RR38 pKa = 11.84  GRR40 pKa = 11.84  SRR42 pKa = 11.84  LAVV45 pKa = 3.35  
 5.47 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.489 
IPC2_protein 11.082 
IPC_protein 12.691 
Toseland    12.852 
ProMoST     13.349 
Dawson      12.852 
Bjellqvist  12.852 
Wikipedia   13.32 
Rodwell     12.427 
Grimsley    12.881 
Solomon     13.349 
Lehninger   13.247 
Nozaki      12.852 
DTASelect   12.852 
Thurlkill   12.852 
EMBOSS      13.349 
Sillero     12.852 
Patrickios  12.149 
IPC_peptide 13.349 
IPC2_peptide  12.34 
IPC2.peptide.svr19  9.145 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2762 
0
2762 
820680
29
2168
297.1
32.14
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        12.301 ± 0.063
1.129 ± 0.016
4.935 ± 0.074
5.709 ± 0.046
2.92 ± 0.032
9.055 ± 0.046
2.118 ± 0.029
3.638 ± 0.033
1.895 ± 0.032
12.869 ± 0.091
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.805 ± 0.019
2.078 ± 0.029
6.456 ± 0.041
4.4 ± 0.038
7.972 ± 0.057
5.602 ± 0.038
4.591 ± 0.033
7.057 ± 0.048
1.709 ± 0.025
1.755 ± 0.024
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here