Cyanobium sp. PCC 7001
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5IKG5|B5IKG5_9CYAN Uncharacterized protein OS=Cyanobium sp. PCC 7001 OX=180281 GN=CPCC7001_1821 PE=4 SV=1
MM1 pKa = 7.8 IDD3 pKa = 3.29 VDD5 pKa = 5.29 DD6 pKa = 6.33 LIDD9 pKa = 4.39 CGSVSTGTLRR19 pKa = 11.84 SSDD22 pKa = 3.87 LADD25 pKa = 3.9 ALLGEE30 pKa = 4.52 IDD32 pKa = 3.94 RR33 pKa = 11.84 LGLATHH39 pKa = 7.0 APDD42 pKa = 4.33 ALIHH46 pKa = 6.24 AAQALACFSSPTVEE60 pKa = 4.87 DD61 pKa = 4.64 LPDD64 pKa = 3.6 NMAEE68 pKa = 4.32 TAGEE72 pKa = 4.37 TVSDD76 pKa = 4.06 MIDD79 pKa = 3.3 WLNDD83 pKa = 3.36 HH84 pKa = 7.1 APDD87 pKa = 4.18 GWCLQTLEE95 pKa = 4.82 GDD97 pKa = 4.05 GADD100 pKa = 5.14 LLWQPVTLRR109 pKa = 11.84 AQLQAAGSPWVQSEE123 pKa = 3.78 WDD125 pKa = 3.48 KK126 pKa = 11.35 RR127 pKa = 11.84 SQNDD131 pKa = 4.01 AIDD134 pKa = 4.98 DD135 pKa = 4.22 IIEE138 pKa = 4.46 RR139 pKa = 11.84 FDD141 pKa = 4.07 DD142 pKa = 4.45 CPEE145 pKa = 4.02 ALQDD149 pKa = 4.11 FDD151 pKa = 3.72 ISDD154 pKa = 3.6 WVNVAEE160 pKa = 5.22 CYY162 pKa = 9.91 TGTLLRR168 pKa = 11.84 RR169 pKa = 11.84 WEE171 pKa = 4.08 LQEE174 pKa = 3.66 QSIRR178 pKa = 11.84 ALFDD182 pKa = 4.47 DD183 pKa = 4.49 YY184 pKa = 11.34 CDD186 pKa = 3.91 QLGCTSALEE195 pKa = 4.23 ALDD198 pKa = 3.99 GQTIEE203 pKa = 5.67 DD204 pKa = 4.49 PDD206 pKa = 4.78 DD207 pKa = 3.45 FTAAYY212 pKa = 10.25 VNLAMTWGAQCLLRR226 pKa = 11.84 EE227 pKa = 4.55 IDD229 pKa = 3.91 PDD231 pKa = 3.7 HH232 pKa = 6.78
Molecular weight: 25.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.579
IPC_protein 3.617
Toseland 3.389
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.579
Rodwell 3.439
Grimsley 3.287
Solomon 3.617
Lehninger 3.579
Nozaki 3.732
DTASelect 4.024
Thurlkill 3.452
EMBOSS 3.592
Sillero 3.745
Patrickios 1.837
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|B5INV1|B5INV1_9CYAN 30S ribosomal protein S4 OS=Cyanobium sp. PCC 7001 OX=180281 GN=rpsD PE=3 SV=1
MM1 pKa = 7.56 TKK3 pKa = 9.03 RR4 pKa = 11.84 TLEE7 pKa = 3.92 GTSRR11 pKa = 11.84 KK12 pKa = 9.24 RR13 pKa = 11.84 KK14 pKa = 7.95 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 THH26 pKa = 5.95 TGRR29 pKa = 11.84 RR30 pKa = 11.84 VIRR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 SRR42 pKa = 11.84 LAVV45 pKa = 3.35
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.489
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.427
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.149
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2762
0
2762
820680
29
2168
297.1
32.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.301 ± 0.063
1.129 ± 0.016
4.935 ± 0.074
5.709 ± 0.046
2.92 ± 0.032
9.055 ± 0.046
2.118 ± 0.029
3.638 ± 0.033
1.895 ± 0.032
12.869 ± 0.091
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.805 ± 0.019
2.078 ± 0.029
6.456 ± 0.041
4.4 ± 0.038
7.972 ± 0.057
5.602 ± 0.038
4.591 ± 0.033
7.057 ± 0.048
1.709 ± 0.025
1.755 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here