Amino acid dipepetide frequency for Cyanobium sp. PCC 7001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.139AlaAla: 18.139 ± 0.235	1.565AlaCys: 1.565 ± 0.045	6.307AlaAsp: 6.307 ± 0.105	7.635AlaGlu: 7.635 ± 0.111	3.335AlaPhe: 3.335 ± 0.062	11.115AlaGly: 11.115 ± 0.123	1.957AlaHis: 1.957 ± 0.052	5.024AlaIle: 5.024 ± 0.089	2.025AlaLys: 2.025 ± 0.061	15.841AlaLeu: 15.841 ± 0.199	2.946AlaMet: 2.946 ± 0.071	2.204AlaAsn: 2.204 ± 0.066	6.702AlaPro: 6.702 ± 0.113	4.184AlaGln: 4.184 ± 0.078	8.347AlaArg: 8.347 ± 0.115	7.047AlaSer: 7.047 ± 0.124	5.392AlaThr: 5.392 ± 0.084	9.285AlaVal: 9.285 ± 0.12	1.991AlaTrp: 1.991 ± 0.054	1.973AlaTyr: 1.973 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.041CysAla: 1.041 ± 0.036	0.223CysCys: 0.223 ± 0.016	0.592CysAsp: 0.592 ± 0.027	0.523CysGlu: 0.523 ± 0.025	0.391CysPhe: 0.391 ± 0.022	1.249CysGly: 1.249 ± 0.037	0.319CysHis: 0.319 ± 0.022	0.352CysIle: 0.352 ± 0.022	0.168CysLys: 0.168 ± 0.014	1.366CysLeu: 1.366 ± 0.045	0.158CysMet: 0.158 ± 0.014	0.252CysAsn: 0.252 ± 0.019	0.74CysPro: 0.74 ± 0.029	0.395CysGln: 0.395 ± 0.023	1.173CysArg: 1.173 ± 0.039	0.727CysSer: 0.727 ± 0.03	0.48CysThr: 0.48 ± 0.026	0.671CysVal: 0.671 ± 0.029	0.257CysTrp: 0.257 ± 0.016	0.216CysTyr: 0.216 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.506AspAla: 5.506 ± 0.109	0.501AspCys: 0.501 ± 0.025	2.749AspAsp: 2.749 ± 0.391	2.192AspGlu: 2.192 ± 0.056	1.44AspPhe: 1.44 ± 0.039	4.993AspGly: 4.993 ± 0.099	1.226AspHis: 1.226 ± 0.042	1.4AspIle: 1.4 ± 0.042	0.825AspLys: 0.825 ± 0.036	6.789AspLeu: 6.789 ± 0.101	0.619AspMet: 0.619 ± 0.028	0.928AspAsn: 0.928 ± 0.037	4.7AspPro: 4.7 ± 0.087	2.258AspGln: 2.258 ± 0.049	4.022AspArg: 4.022 ± 0.084	2.138AspSer: 2.138 ± 0.059	2.177AspThr: 2.177 ± 0.056	2.91AspVal: 2.91 ± 0.061	0.95AspTrp: 0.95 ± 0.038	1.021AspTyr: 1.021 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	8.882GluAla: 8.882 ± 0.123	0.369GluCys: 0.369 ± 0.022	2.213GluAsp: 2.213 ± 0.054	3.344GluGlu: 3.344 ± 0.077	1.177GluPhe: 1.177 ± 0.04	4.801GluGly: 4.801 ± 0.082	1.199GluHis: 1.199 ± 0.035	1.944GluIle: 1.944 ± 0.054	1.229GluLys: 1.229 ± 0.051	7.714GluLeu: 7.714 ± 0.111	0.808GluMet: 0.808 ± 0.033	0.905GluAsn: 0.905 ± 0.037	3.771GluPro: 3.771 ± 0.067	3.39GluGln: 3.39 ± 0.073	5.807GluArg: 5.807 ± 0.089	2.394GluSer: 2.394 ± 0.051	2.184GluThr: 2.184 ± 0.052	3.799GluVal: 3.799 ± 0.073	0.612GluTrp: 0.612 ± 0.027	0.543GluTyr: 0.543 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	2.927PheAla: 2.927 ± 0.065	0.464PheCys: 0.464 ± 0.024	1.842PheAsp: 1.842 ± 0.051	1.638PheGlu: 1.638 ± 0.044	1.0PhePhe: 1.0 ± 0.038	2.945PheGly: 2.945 ± 0.066	0.687PheHis: 0.687 ± 0.03	0.998PheIle: 0.998 ± 0.042	0.563PheLys: 0.563 ± 0.029	3.117PheLeu: 3.117 ± 0.068	0.491PheMet: 0.491 ± 0.025	0.92PheAsn: 0.92 ± 0.034	1.454PhePro: 1.454 ± 0.041	1.128PheGln: 1.128 ± 0.034	2.176PheArg: 2.176 ± 0.057	2.109PheSer: 2.109 ± 0.055	1.657PheThr: 1.657 ± 0.044	1.846PheVal: 1.846 ± 0.058	0.526PheTrp: 0.526 ± 0.03	0.704PheTyr: 0.704 ± 0.034	0.001PheXaa: 0.001 ± 0.001
Gly	9.041GlyAla: 9.041 ± 0.129	1.29GlyCys: 1.29 ± 0.043	4.536GlyAsp: 4.536 ± 0.098	4.93GlyGlu: 4.93 ± 0.084	3.218GlyPhe: 3.218 ± 0.066	8.121GlyGly: 8.121 ± 0.137	1.924GlyHis: 1.924 ± 0.049	3.698GlyIle: 3.698 ± 0.076	2.04GlyLys: 2.04 ± 0.058	11.751GlyLeu: 11.751 ± 0.146	2.089GlyMet: 2.089 ± 0.063	1.907GlyAsn: 1.907 ± 0.067	4.49GlyPro: 4.49 ± 0.083	3.754GlyGln: 3.754 ± 0.075	7.062GlyArg: 7.062 ± 0.103	6.008GlySer: 6.008 ± 0.105	4.394GlyThr: 4.394 ± 0.099	6.463GlyVal: 6.463 ± 0.09	1.95GlyTrp: 1.95 ± 0.046	1.875GlyTyr: 1.875 ± 0.045	0.001GlyXaa: 0.001 ± 0.001
His	1.979HisAla: 1.979 ± 0.055	0.366HisCys: 0.366 ± 0.023	0.997HisAsp: 0.997 ± 0.034	0.821HisGlu: 0.821 ± 0.033	0.589HisPhe: 0.589 ± 0.027	2.227HisGly: 2.227 ± 0.054	0.911HisHis: 0.911 ± 0.041	0.632HisIle: 0.632 ± 0.028	0.284HisLys: 0.284 ± 0.019	2.586HisLeu: 2.586 ± 0.056	0.253HisMet: 0.253 ± 0.017	0.444HisAsn: 0.444 ± 0.024	1.894HisPro: 1.894 ± 0.051	0.958HisGln: 0.958 ± 0.037	2.057HisArg: 2.057 ± 0.055	1.099HisSer: 1.099 ± 0.04	0.937HisThr: 0.937 ± 0.034	1.071HisVal: 1.071 ± 0.036	0.552HisTrp: 0.552 ± 0.024	0.523HisTyr: 0.523 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	4.65IleAla: 4.65 ± 0.075	0.444IleCys: 0.444 ± 0.024	2.425IleAsp: 2.425 ± 0.059	2.364IleGlu: 2.364 ± 0.063	0.928IlePhe: 0.928 ± 0.035	3.821IleGly: 3.821 ± 0.064	0.881IleHis: 0.881 ± 0.039	0.853IleIle: 0.853 ± 0.035	0.827IleLys: 0.827 ± 0.035	3.125IleLeu: 3.125 ± 0.07	0.311IleMet: 0.311 ± 0.025	1.138IleAsn: 1.138 ± 0.043	2.315IlePro: 2.315 ± 0.058	1.451IleGln: 1.451 ± 0.037	2.782IleArg: 2.782 ± 0.059	2.237IleSer: 2.237 ± 0.063	2.41IleThr: 2.41 ± 0.061	2.176IleVal: 2.176 ± 0.054	0.498IleTrp: 0.498 ± 0.026	0.738IleTyr: 0.738 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	2.671LysAla: 2.671 ± 0.063	0.116LysCys: 0.116 ± 0.012	0.936LysAsp: 0.936 ± 0.036	1.077LysGlu: 1.077 ± 0.047	0.445LysPhe: 0.445 ± 0.024	1.746LysGly: 1.746 ± 0.056	0.344LysHis: 0.344 ± 0.023	0.69LysIle: 0.69 ± 0.032	0.586LysLys: 0.586 ± 0.041	2.215LysLeu: 2.215 ± 0.06	0.26LysMet: 0.26 ± 0.017	0.406LysAsn: 0.406 ± 0.025	1.353LysPro: 1.353 ± 0.052	0.818LysGln: 0.818 ± 0.039	1.451LysArg: 1.451 ± 0.047	0.877LysSer: 0.877 ± 0.037	1.042LysThr: 1.042 ± 0.044	1.426LysVal: 1.426 ± 0.048	0.158LysTrp: 0.158 ± 0.014	0.331LysTyr: 0.331 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	16.931LeuAla: 16.931 ± 0.204	1.318LeuCys: 1.318 ± 0.036	6.256LeuAsp: 6.256 ± 0.099	8.889LeuGlu: 8.889 ± 0.138	3.214LeuPhe: 3.214 ± 0.069	10.681LeuGly: 10.681 ± 0.129	2.598LeuHis: 2.598 ± 0.055	4.656LeuIle: 4.656 ± 0.087	2.388LeuLys: 2.388 ± 0.059	16.86LeuLeu: 16.86 ± 0.227	2.182LeuMet: 2.182 ± 0.056	2.517LeuAsn: 2.517 ± 0.064	8.209LeuPro: 8.209 ± 0.117	6.342LeuGln: 6.342 ± 0.098	9.989LeuArg: 9.989 ± 0.125	6.698LeuSer: 6.698 ± 0.099	5.474LeuThr: 5.474 ± 0.085	9.755LeuVal: 9.755 ± 0.13	1.833LeuTrp: 1.833 ± 0.054	1.9LeuTyr: 1.9 ± 0.052	0.002LeuXaa: 0.002 ± 0.002
Met	3.211MetAla: 3.211 ± 0.063	0.071MetCys: 0.071 ± 0.009	0.785MetAsp: 0.785 ± 0.034	1.098MetGlu: 1.098 ± 0.034	0.341MetPhe: 0.341 ± 0.02	1.718MetGly: 1.718 ± 0.054	0.317MetHis: 0.317 ± 0.024	0.604MetIle: 0.604 ± 0.028	0.414MetLys: 0.414 ± 0.023	1.915MetLeu: 1.915 ± 0.047	0.228MetMet: 0.228 ± 0.016	0.403MetAsn: 0.403 ± 0.022	1.329MetPro: 1.329 ± 0.042	0.677MetGln: 0.677 ± 0.029	1.145MetArg: 1.145 ± 0.039	0.986MetSer: 0.986 ± 0.037	1.022MetThr: 1.022 ± 0.032	1.513MetVal: 1.513 ± 0.042	0.095MetTrp: 0.095 ± 0.011	0.179MetTyr: 0.179 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.337AsnAla: 2.337 ± 0.073	0.214AsnCys: 0.214 ± 0.017	1.041AsnAsp: 1.041 ± 0.048	0.723AsnGlu: 0.723 ± 0.033	0.626AsnPhe: 0.626 ± 0.034	2.128AsnGly: 2.128 ± 0.074	0.492AsnHis: 0.492 ± 0.026	0.662AsnIle: 0.662 ± 0.03	0.433AsnLys: 0.433 ± 0.026	2.661AsnLeu: 2.661 ± 0.061	0.29AsnMet: 0.29 ± 0.02	0.58AsnAsn: 0.58 ± 0.033	1.822AsnPro: 1.822 ± 0.045	0.827AsnGln: 0.827 ± 0.035	1.795AsnArg: 1.795 ± 0.049	1.027AsnSer: 1.027 ± 0.036	1.052AsnThr: 1.052 ± 0.037	1.292AsnVal: 1.292 ± 0.042	0.341AsnTrp: 0.341 ± 0.022	0.437AsnTyr: 0.437 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	8.782ProAla: 8.782 ± 0.129	0.592ProCys: 0.592 ± 0.03	3.584ProAsp: 3.584 ± 0.074	4.641ProGlu: 4.641 ± 0.079	1.872ProPhe: 1.872 ± 0.054	6.188ProGly: 6.188 ± 0.095	1.201ProHis: 1.201 ± 0.039	2.076ProIle: 2.076 ± 0.053	0.944ProLys: 0.944 ± 0.035	7.835ProLeu: 7.835 ± 0.129	1.371ProMet: 1.371 ± 0.046	1.075ProAsn: 1.075 ± 0.038	4.399ProPro: 4.399 ± 0.115	2.441ProGln: 2.441 ± 0.057	3.981ProArg: 3.981 ± 0.077	3.847ProSer: 3.847 ± 0.074	2.844ProThr: 2.844 ± 0.062	4.602ProVal: 4.602 ± 0.088	1.237ProTrp: 1.237 ± 0.045	1.052ProTyr: 1.052 ± 0.036	0.001ProXaa: 0.001 ± 0.001
Gln	5.882GlnAla: 5.882 ± 0.1	0.331GlnCys: 0.331 ± 0.023	1.554GlnAsp: 1.554 ± 0.038	2.578GlnGlu: 2.578 ± 0.062	0.963GlnPhe: 0.963 ± 0.036	3.798GlnGly: 3.798 ± 0.069	0.9GlnHis: 0.9 ± 0.037	1.48GlnIle: 1.48 ± 0.041	0.842GlnLys: 0.842 ± 0.033	6.339GlnLeu: 6.339 ± 0.095	0.647GlnMet: 0.647 ± 0.029	0.675GlnAsn: 0.675 ± 0.03	3.133GlnPro: 3.133 ± 0.067	2.654GlnGln: 2.654 ± 0.081	4.449GlnArg: 4.449 ± 0.075	1.894GlnSer: 1.894 ± 0.047	1.454GlnThr: 1.454 ± 0.043	3.297GlnVal: 3.297 ± 0.059	0.659GlnTrp: 0.659 ± 0.029	0.476GlnTyr: 0.476 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.129ArgAla: 7.129 ± 0.086	1.088ArgCys: 1.088 ± 0.039	3.48ArgAsp: 3.48 ± 0.069	4.037ArgGlu: 4.037 ± 0.071	3.007ArgPhe: 3.007 ± 0.062	5.57ArgGly: 5.57 ± 0.094	2.253ArgHis: 2.253 ± 0.055	3.189ArgIle: 3.189 ± 0.059	1.332ArgLys: 1.332 ± 0.049	11.406ArgLeu: 11.406 ± 0.159	1.506ArgMet: 1.506 ± 0.041	1.651ArgAsn: 1.651 ± 0.047	4.867ArgPro: 4.867 ± 0.092	4.846ArgGln: 4.846 ± 0.104	8.013ArgArg: 8.013 ± 0.124	4.83ArgSer: 4.83 ± 0.085	3.111ArgThr: 3.111 ± 0.066	4.69ArgVal: 4.69 ± 0.09	2.013ArgTrp: 2.013 ± 0.051	1.697ArgTyr: 1.697 ± 0.042	0.001ArgXaa: 0.001 ± 0.001
Ser	5.962SerAla: 5.962 ± 0.101	0.698SerCys: 0.698 ± 0.031	2.761SerAsp: 2.761 ± 0.077	2.647SerGlu: 2.647 ± 0.057	1.908SerPhe: 1.908 ± 0.053	6.108SerGly: 6.108 ± 0.11	1.181SerHis: 1.181 ± 0.039	1.886SerIle: 1.886 ± 0.058	0.977SerLys: 0.977 ± 0.04	7.003SerLeu: 7.003 ± 0.1	1.044SerMet: 1.044 ± 0.037	1.149SerAsn: 1.149 ± 0.036	3.649SerPro: 3.649 ± 0.07	2.207SerGln: 2.207 ± 0.051	4.555SerArg: 4.555 ± 0.073	3.781SerSer: 3.781 ± 0.09	2.895SerThr: 2.895 ± 0.066	3.425SerVal: 3.425 ± 0.075	1.048SerTrp: 1.048 ± 0.04	1.137SerTyr: 1.137 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	6.057ThrAla: 6.057 ± 0.088	0.45ThrCys: 0.45 ± 0.024	2.128ThrAsp: 2.128 ± 0.052	2.05ThrGlu: 2.05 ± 0.049	1.359ThrPhe: 1.359 ± 0.046	4.426ThrGly: 4.426 ± 0.084	0.766ThrHis: 0.766 ± 0.032	1.844ThrIle: 1.844 ± 0.05	0.816ThrLys: 0.816 ± 0.039	5.8ThrLeu: 5.8 ± 0.098	0.773ThrMet: 0.773 ± 0.032	0.997ThrAsn: 0.997 ± 0.043	3.645ThrPro: 3.645 ± 0.072	1.26ThrGln: 1.26 ± 0.041	2.72ThrArg: 2.72 ± 0.061	2.645ThrSer: 2.645 ± 0.064	2.787ThrThr: 2.787 ± 0.082	3.68ThrVal: 3.68 ± 0.064	0.677ThrTrp: 0.677 ± 0.03	1.03ThrTyr: 1.03 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	8.543ValAla: 8.543 ± 0.115	0.727ValCys: 0.727 ± 0.027	3.563ValAsp: 3.563 ± 0.064	4.366ValGlu: 4.366 ± 0.073	2.254ValPhe: 2.254 ± 0.053	5.576ValGly: 5.576 ± 0.089	1.265ValHis: 1.265 ± 0.045	3.117ValIle: 3.117 ± 0.071	1.549ValLys: 1.549 ± 0.048	9.568ValLeu: 9.568 ± 0.128	1.42ValMet: 1.42 ± 0.048	1.8ValAsn: 1.8 ± 0.053	4.008ValPro: 4.008 ± 0.068	2.664ValGln: 2.664 ± 0.053	4.717ValArg: 4.717 ± 0.088	3.697ValSer: 3.697 ± 0.079	3.208ValThr: 3.208 ± 0.079	6.514ValVal: 6.514 ± 0.116	0.949ValTrp: 0.949 ± 0.032	1.07ValTyr: 1.07 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.471TrpAla: 1.471 ± 0.048	0.24TrpCys: 0.24 ± 0.017	0.624TrpAsp: 0.624 ± 0.031	0.74TrpGlu: 0.74 ± 0.033	0.55TrpPhe: 0.55 ± 0.028	1.296TrpGly: 1.296 ± 0.038	0.418TrpHis: 0.418 ± 0.022	0.707TrpIle: 0.707 ± 0.03	0.35TrpLys: 0.35 ± 0.02	2.77TrpLeu: 2.77 ± 0.069	0.408TrpMet: 0.408 ± 0.024	0.39TrpAsn: 0.39 ± 0.023	1.061TrpPro: 1.061 ± 0.044	1.091TrpGln: 1.091 ± 0.047	1.725TrpArg: 1.725 ± 0.049	0.985TrpSer: 0.985 ± 0.039	0.617TrpThr: 0.617 ± 0.027	0.991TrpVal: 0.991 ± 0.036	0.401TrpTrp: 0.401 ± 0.022	0.258TrpTyr: 0.258 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.87TyrAla: 1.87 ± 0.048	0.227TyrCys: 0.227 ± 0.016	0.976TyrAsp: 0.976 ± 0.034	0.794TyrGlu: 0.794 ± 0.032	0.578TyrPhe: 0.578 ± 0.028	2.037TyrGly: 2.037 ± 0.056	0.339TyrHis: 0.339 ± 0.023	0.564TyrIle: 0.564 ± 0.026	0.353TyrLys: 0.353 ± 0.021	2.032TyrLeu: 2.032 ± 0.054	0.247TyrMet: 0.247 ± 0.017	0.437TyrAsn: 0.437 ± 0.023	1.025TyrPro: 1.025 ± 0.037	0.659TyrGln: 0.659 ± 0.031	1.749TyrArg: 1.749 ± 0.057	0.993TyrSer: 0.993 ± 0.035	0.771TyrThr: 0.771 ± 0.034	1.166TyrVal: 1.166 ± 0.038	0.342TyrTrp: 0.342 ± 0.021	0.387TyrTyr: 0.387 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.057XaaXaa: 0.057 ± 0.024

Statistics based on 2762 proteins (820680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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