Escherichia phage N7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M8E1|A0A3G3M8E1_9CAUD Uncharacterized protein OS=Escherichia phage N7 OX=2340720 PE=4 SV=1
MM1 pKa = 7.68LSPSQSLQYY10 pKa = 10.38QKK12 pKa = 11.19EE13 pKa = 4.4SVEE16 pKa = 4.33RR17 pKa = 11.84ALTCANCGQKK27 pKa = 10.32LHH29 pKa = 5.86VLEE32 pKa = 4.31VHH34 pKa = 6.17VCSDD38 pKa = 4.12CCAEE42 pKa = 5.29LMSDD46 pKa = 4.35PNSSMYY52 pKa = 10.6EE53 pKa = 3.98EE54 pKa = 5.0EE55 pKa = 4.99DD56 pKa = 4.39DD57 pKa = 4.1EE58 pKa = 4.75

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M8D1|A0A3G3M8D1_9CAUD Protein ninF OS=Escherichia phage N7 OX=2340720 PE=4 SV=1
MM1 pKa = 7.07TVVITYY7 pKa = 9.95LADD10 pKa = 4.18DD11 pKa = 3.61NARR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84QAQRR25 pKa = 11.84EE26 pKa = 3.88QAMQEE31 pKa = 3.48QRR33 pKa = 11.84LARR36 pKa = 11.84KK37 pKa = 8.86IALKK41 pKa = 10.84LSGCVRR47 pKa = 11.84ADD49 pKa = 3.17KK50 pKa = 10.69AASLGSLRR58 pKa = 11.84CKK60 pKa = 9.97KK61 pKa = 9.03TDD63 pKa = 3.19EE64 pKa = 4.41VEE66 pKa = 4.11RR67 pKa = 11.84KK68 pKa = 7.72QNRR71 pKa = 11.84IYY73 pKa = 10.3YY74 pKa = 8.1RR75 pKa = 11.84KK76 pKa = 8.79PRR78 pKa = 11.84SEE80 pKa = 3.97MGVTCVGRR88 pKa = 11.84QKK90 pKa = 10.7QRR92 pKa = 11.84GKK94 pKa = 10.65SIPAYY99 pKa = 10.47YY100 pKa = 10.36DD101 pKa = 2.97

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11181

50

709

199.7

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.489 ± 0.53

1.136 ± 0.145

5.617 ± 0.243

6.985 ± 0.537

3.229 ± 0.174

6.806 ± 0.333

1.538 ± 0.155

6.261 ± 0.279

6.493 ± 0.357

7.504 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.193

5.134 ± 0.436

3.891 ± 0.18

5.205 ± 0.389

5.822 ± 0.332

6.386 ± 0.324

5.268 ± 0.332

5.903 ± 0.314

1.494 ± 0.15

3.032 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski