Amino acid dipepetide frequency for Escherichia phage N7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.123AlaAla: 9.123 ± 1.054
0.447AlaCys: 0.447 ± 0.191
6.082AlaAsp: 6.082 ± 0.602
7.424AlaGlu: 7.424 ± 1.385
2.862AlaPhe: 2.862 ± 0.483
5.725AlaGly: 5.725 ± 0.921
1.431AlaHis: 1.431 ± 0.381
6.619AlaIle: 6.619 ± 0.712
6.44AlaLys: 6.44 ± 0.967
6.53AlaLeu: 6.53 ± 0.876
3.846AlaMet: 3.846 ± 0.629
4.383AlaAsn: 4.383 ± 0.797
2.415AlaPro: 2.415 ± 0.569
5.903AlaGln: 5.903 ± 1.3
5.277AlaArg: 5.277 ± 0.703
5.098AlaSer: 5.098 ± 0.628
6.351AlaThr: 6.351 ± 0.969
5.009AlaVal: 5.009 ± 0.697
1.342AlaTrp: 1.342 ± 0.271
2.504AlaTyr: 2.504 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
1.252CysAla: 1.252 ± 0.382
0.179CysCys: 0.179 ± 0.128
0.268CysAsp: 0.268 ± 0.132
0.537CysGlu: 0.537 ± 0.209
0.447CysPhe: 0.447 ± 0.214
1.252CysGly: 1.252 ± 0.362
0.358CysHis: 0.358 ± 0.191
0.626CysIle: 0.626 ± 0.226
0.716CysLys: 0.716 ± 0.258
0.626CysLeu: 0.626 ± 0.275
0.358CysMet: 0.358 ± 0.169
0.358CysAsn: 0.358 ± 0.167
0.626CysPro: 0.626 ± 0.255
0.358CysGln: 0.358 ± 0.166
0.805CysArg: 0.805 ± 0.292
0.894CysSer: 0.894 ± 0.303
0.358CysThr: 0.358 ± 0.238
0.894CysVal: 0.894 ± 0.31
0.268CysTrp: 0.268 ± 0.148
0.179CysTyr: 0.179 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
5.635AspAla: 5.635 ± 0.52
0.894AspCys: 0.894 ± 0.307
3.667AspAsp: 3.667 ± 0.622
4.025AspGlu: 4.025 ± 0.797
1.789AspPhe: 1.789 ± 0.437
4.293AspGly: 4.293 ± 0.561
1.163AspHis: 1.163 ± 0.333
4.293AspIle: 4.293 ± 0.499
3.309AspLys: 3.309 ± 0.538
3.757AspLeu: 3.757 ± 0.532
1.342AspMet: 1.342 ± 0.34
3.131AspAsn: 3.131 ± 0.449
2.236AspPro: 2.236 ± 0.571
1.431AspGln: 1.431 ± 0.365
3.309AspArg: 3.309 ± 0.599
3.578AspSer: 3.578 ± 0.585
1.878AspThr: 1.878 ± 0.483
3.757AspVal: 3.757 ± 0.569
0.805AspTrp: 0.805 ± 0.216
1.878AspTyr: 1.878 ± 0.479
0.0AspXaa: 0.0 ± 0.0
Glu
6.977GluAla: 6.977 ± 1.41
0.894GluCys: 0.894 ± 0.283
2.952GluAsp: 2.952 ± 0.468
4.383GluGlu: 4.383 ± 1.113
2.415GluPhe: 2.415 ± 0.463
3.309GluGly: 3.309 ± 0.565
1.431GluHis: 1.431 ± 0.33
4.651GluIle: 4.651 ± 0.752
4.741GluLys: 4.741 ± 0.706
6.261GluLeu: 6.261 ± 0.741
2.147GluMet: 2.147 ± 0.356
3.309GluAsn: 3.309 ± 0.586
2.683GluPro: 2.683 ± 0.564
4.383GluGln: 4.383 ± 0.618
4.293GluArg: 4.293 ± 0.94
3.578GluSer: 3.578 ± 0.752
3.131GluThr: 3.131 ± 0.763
4.383GluVal: 4.383 ± 0.846
1.699GluTrp: 1.699 ± 0.427
2.236GluTyr: 2.236 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
3.399PheAla: 3.399 ± 0.642
0.447PheCys: 0.447 ± 0.148
2.057PheAsp: 2.057 ± 0.462
1.878PheGlu: 1.878 ± 0.41
1.163PhePhe: 1.163 ± 0.401
2.236PheGly: 2.236 ± 0.467
0.358PheHis: 0.358 ± 0.193
2.862PheIle: 2.862 ± 0.492
2.236PheLys: 2.236 ± 0.406
2.326PheLeu: 2.326 ± 0.433
0.716PheMet: 0.716 ± 0.261
1.878PheAsn: 1.878 ± 0.341
0.894PhePro: 0.894 ± 0.243
0.537PheGln: 0.537 ± 0.208
1.61PheArg: 1.61 ± 0.376
2.594PheSer: 2.594 ± 0.482
2.862PheThr: 2.862 ± 0.553
1.431PheVal: 1.431 ± 0.405
0.268PheTrp: 0.268 ± 0.143
0.537PheTyr: 0.537 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
5.903GlyAla: 5.903 ± 0.887
1.073GlyCys: 1.073 ± 0.302
3.846GlyAsp: 3.846 ± 0.688
4.472GlyGlu: 4.472 ± 0.602
2.862GlyPhe: 2.862 ± 0.451
4.651GlyGly: 4.651 ± 0.943
1.073GlyHis: 1.073 ± 0.356
4.204GlyIle: 4.204 ± 0.577
5.546GlyLys: 5.546 ± 0.702
4.383GlyLeu: 4.383 ± 0.786
1.163GlyMet: 1.163 ± 0.259
3.936GlyAsn: 3.936 ± 1.003
1.431GlyPro: 1.431 ± 0.409
3.936GlyGln: 3.936 ± 0.811
4.383GlyArg: 4.383 ± 0.684
4.114GlySer: 4.114 ± 0.566
3.399GlyThr: 3.399 ± 0.604
4.651GlyVal: 4.651 ± 0.672
0.894GlyTrp: 0.894 ± 0.385
2.147GlyTyr: 2.147 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
1.521HisAla: 1.521 ± 0.365
0.358HisCys: 0.358 ± 0.163
0.805HisAsp: 0.805 ± 0.29
1.521HisGlu: 1.521 ± 0.541
0.626HisPhe: 0.626 ± 0.259
1.431HisGly: 1.431 ± 0.349
0.0HisHis: 0.0 ± 0.0
0.984HisIle: 0.984 ± 0.321
1.163HisLys: 1.163 ± 0.257
1.073HisLeu: 1.073 ± 0.313
0.268HisMet: 0.268 ± 0.156
0.626HisAsn: 0.626 ± 0.239
0.447HisPro: 0.447 ± 0.177
0.716HisGln: 0.716 ± 0.24
0.626HisArg: 0.626 ± 0.249
0.894HisSer: 0.894 ± 0.253
0.179HisThr: 0.179 ± 0.134
0.984HisVal: 0.984 ± 0.368
0.358HisTrp: 0.358 ± 0.146
0.805HisTyr: 0.805 ± 0.278
0.0HisXaa: 0.0 ± 0.0
Ile
6.44IleAla: 6.44 ± 0.805
0.984IleCys: 0.984 ± 0.298
4.472IleAsp: 4.472 ± 0.49
4.025IleGlu: 4.025 ± 0.756
0.984IlePhe: 0.984 ± 0.275
4.919IleGly: 4.919 ± 0.828
1.073IleHis: 1.073 ± 0.345
3.846IleIle: 3.846 ± 0.619
3.22IleLys: 3.22 ± 0.543
3.578IleLeu: 3.578 ± 0.448
1.073IleMet: 1.073 ± 0.302
3.578IleAsn: 3.578 ± 0.631
3.041IlePro: 3.041 ± 0.437
3.22IleGln: 3.22 ± 0.532
4.919IleArg: 4.919 ± 0.681
4.114IleSer: 4.114 ± 0.488
3.667IleThr: 3.667 ± 0.456
2.683IleVal: 2.683 ± 0.406
0.984IleTrp: 0.984 ± 0.382
1.789IleTyr: 1.789 ± 0.34
0.0IleXaa: 0.0 ± 0.0
Lys
6.708LysAla: 6.708 ± 1.157
0.626LysCys: 0.626 ± 0.271
3.131LysAsp: 3.131 ± 0.444
5.903LysGlu: 5.903 ± 0.979
2.415LysPhe: 2.415 ± 0.416
4.204LysGly: 4.204 ± 0.544
0.805LysHis: 0.805 ± 0.295
3.399LysIle: 3.399 ± 0.677
4.293LysLys: 4.293 ± 0.761
6.172LysLeu: 6.172 ± 0.672
1.878LysMet: 1.878 ± 0.401
2.236LysAsn: 2.236 ± 0.48
3.309LysPro: 3.309 ± 0.587
3.309LysGln: 3.309 ± 0.661
3.936LysArg: 3.936 ± 0.665
3.309LysSer: 3.309 ± 0.469
2.683LysThr: 2.683 ± 0.506
3.309LysVal: 3.309 ± 0.565
0.716LysTrp: 0.716 ± 0.235
2.594LysTyr: 2.594 ± 0.596
0.0LysXaa: 0.0 ± 0.0
Leu
8.318LeuAla: 8.318 ± 1.069
0.626LeuCys: 0.626 ± 0.204
4.383LeuAsp: 4.383 ± 0.584
6.261LeuGlu: 6.261 ± 0.789
2.057LeuPhe: 2.057 ± 0.38
3.757LeuGly: 3.757 ± 0.858
0.805LeuHis: 0.805 ± 0.308
4.383LeuIle: 4.383 ± 0.551
5.546LeuLys: 5.546 ± 0.818
5.456LeuLeu: 5.456 ± 0.638
2.236LeuMet: 2.236 ± 0.447
3.309LeuAsn: 3.309 ± 0.497
3.667LeuPro: 3.667 ± 0.565
2.862LeuGln: 2.862 ± 0.566
4.651LeuArg: 4.651 ± 0.669
5.725LeuSer: 5.725 ± 0.657
4.293LeuThr: 4.293 ± 0.501
3.757LeuVal: 3.757 ± 0.544
0.894LeuTrp: 0.894 ± 0.238
2.057LeuTyr: 2.057 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
3.131MetAla: 3.131 ± 0.489
0.268MetCys: 0.268 ± 0.169
1.431MetAsp: 1.431 ± 0.285
1.968MetGlu: 1.968 ± 0.419
0.894MetPhe: 0.894 ± 0.267
0.894MetGly: 0.894 ± 0.313
0.268MetHis: 0.268 ± 0.149
1.431MetIle: 1.431 ± 0.376
1.878MetLys: 1.878 ± 0.481
2.594MetLeu: 2.594 ± 0.472
0.716MetMet: 0.716 ± 0.251
1.431MetAsn: 1.431 ± 0.306
1.252MetPro: 1.252 ± 0.315
1.61MetGln: 1.61 ± 0.363
1.431MetArg: 1.431 ± 0.377
2.415MetSer: 2.415 ± 0.442
2.057MetThr: 2.057 ± 0.355
1.431MetVal: 1.431 ± 0.325
0.268MetTrp: 0.268 ± 0.14
0.716MetTyr: 0.716 ± 0.275
0.0MetXaa: 0.0 ± 0.0
Asn
4.562AsnAla: 4.562 ± 0.804
0.358AsnCys: 0.358 ± 0.199
1.968AsnAsp: 1.968 ± 0.5
3.309AsnGlu: 3.309 ± 0.609
0.984AsnPhe: 0.984 ± 0.366
4.651AsnGly: 4.651 ± 0.671
1.521AsnHis: 1.521 ± 0.355
2.504AsnIle: 2.504 ± 0.393
3.488AsnLys: 3.488 ± 0.588
3.667AsnLeu: 3.667 ± 0.461
1.163AsnMet: 1.163 ± 0.275
3.488AsnAsn: 3.488 ± 0.706
3.757AsnPro: 3.757 ± 0.69
2.236AsnGln: 2.236 ± 0.442
2.773AsnArg: 2.773 ± 0.44
3.22AsnSer: 3.22 ± 0.829
2.326AsnThr: 2.326 ± 0.418
2.773AsnVal: 2.773 ± 0.487
0.537AsnTrp: 0.537 ± 0.223
2.057AsnTyr: 2.057 ± 0.683
0.0AsnXaa: 0.0 ± 0.0
Pro
3.578ProAla: 3.578 ± 0.535
0.089ProCys: 0.089 ± 0.094
2.862ProAsp: 2.862 ± 0.388
4.114ProGlu: 4.114 ± 0.578
1.342ProPhe: 1.342 ± 0.444
2.415ProGly: 2.415 ± 0.472
0.268ProHis: 0.268 ± 0.196
2.326ProIle: 2.326 ± 0.411
2.594ProLys: 2.594 ± 0.476
2.504ProLeu: 2.504 ± 0.548
1.431ProMet: 1.431 ± 0.36
1.789ProAsn: 1.789 ± 0.397
1.342ProPro: 1.342 ± 0.288
2.057ProGln: 2.057 ± 0.408
1.789ProArg: 1.789 ± 0.361
3.131ProSer: 3.131 ± 0.527
2.147ProThr: 2.147 ± 0.399
2.236ProVal: 2.236 ± 0.485
0.358ProTrp: 0.358 ± 0.148
0.537ProTyr: 0.537 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
4.83GlnAla: 4.83 ± 0.793
0.447GlnCys: 0.447 ± 0.18
1.699GlnAsp: 1.699 ± 0.411
2.952GlnGlu: 2.952 ± 0.693
2.862GlnPhe: 2.862 ± 0.514
2.415GlnGly: 2.415 ± 0.428
0.626GlnHis: 0.626 ± 0.304
3.488GlnIle: 3.488 ± 0.425
2.862GlnLys: 2.862 ± 0.414
3.936GlnLeu: 3.936 ± 0.662
1.789GlnMet: 1.789 ± 0.405
2.147GlnAsn: 2.147 ± 0.657
1.878GlnPro: 1.878 ± 0.441
5.635GlnGln: 5.635 ± 1.459
3.309GlnArg: 3.309 ± 0.625
3.757GlnSer: 3.757 ± 0.701
2.683GlnThr: 2.683 ± 0.508
2.415GlnVal: 2.415 ± 0.455
1.073GlnTrp: 1.073 ± 0.272
1.252GlnTyr: 1.252 ± 0.433
0.0GlnXaa: 0.0 ± 0.0
Arg
5.009ArgAla: 5.009 ± 0.784
0.805ArgCys: 0.805 ± 0.272
2.952ArgAsp: 2.952 ± 0.593
3.846ArgGlu: 3.846 ± 0.894
1.342ArgPhe: 1.342 ± 0.258
4.293ArgGly: 4.293 ± 0.649
1.342ArgHis: 1.342 ± 0.339
4.562ArgIle: 4.562 ± 0.718
5.098ArgLys: 5.098 ± 0.841
4.204ArgLeu: 4.204 ± 0.502
1.61ArgMet: 1.61 ± 0.445
3.399ArgAsn: 3.399 ± 0.575
1.252ArgPro: 1.252 ± 0.322
3.22ArgGln: 3.22 ± 0.529
3.399ArgArg: 3.399 ± 0.78
3.131ArgSer: 3.131 ± 0.499
1.878ArgThr: 1.878 ± 0.423
3.846ArgVal: 3.846 ± 0.588
1.073ArgTrp: 1.073 ± 0.316
1.968ArgTyr: 1.968 ± 0.459
0.0ArgXaa: 0.0 ± 0.0
Ser
5.009SerAla: 5.009 ± 0.879
0.358SerCys: 0.358 ± 0.174
3.936SerAsp: 3.936 ± 0.512
4.025SerGlu: 4.025 ± 0.6
2.594SerPhe: 2.594 ± 0.449
5.814SerGly: 5.814 ± 0.896
0.716SerHis: 0.716 ± 0.233
3.131SerIle: 3.131 ± 0.44
3.131SerLys: 3.131 ± 0.666
5.814SerLeu: 5.814 ± 0.603
2.236SerMet: 2.236 ± 0.522
2.952SerAsn: 2.952 ± 0.414
3.131SerPro: 3.131 ± 0.525
4.025SerGln: 4.025 ± 0.601
3.22SerArg: 3.22 ± 0.554
3.041SerSer: 3.041 ± 0.69
2.683SerThr: 2.683 ± 0.576
5.009SerVal: 5.009 ± 0.673
1.252SerTrp: 1.252 ± 0.283
1.789SerTyr: 1.789 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
4.919ThrAla: 4.919 ± 0.731
0.894ThrCys: 0.894 ± 0.289
4.114ThrAsp: 4.114 ± 0.567
3.041ThrGlu: 3.041 ± 0.486
1.521ThrPhe: 1.521 ± 0.478
4.741ThrGly: 4.741 ± 0.63
0.537ThrHis: 0.537 ± 0.197
3.309ThrIle: 3.309 ± 0.754
2.862ThrLys: 2.862 ± 0.437
3.131ThrLeu: 3.131 ± 0.688
0.984ThrMet: 0.984 ± 0.25
2.504ThrAsn: 2.504 ± 0.438
2.504ThrPro: 2.504 ± 0.59
1.878ThrGln: 1.878 ± 0.334
2.236ThrArg: 2.236 ± 0.599
3.22ThrSer: 3.22 ± 0.506
3.399ThrThr: 3.399 ± 0.622
4.293ThrVal: 4.293 ± 0.609
0.894ThrTrp: 0.894 ± 0.265
1.699ThrTyr: 1.699 ± 0.444
0.0ThrXaa: 0.0 ± 0.0
Val
4.741ValAla: 4.741 ± 0.673
0.894ValCys: 0.894 ± 0.275
2.236ValAsp: 2.236 ± 0.432
4.114ValGlu: 4.114 ± 0.472
1.61ValPhe: 1.61 ± 0.427
4.204ValGly: 4.204 ± 0.606
0.894ValHis: 0.894 ± 0.272
4.472ValIle: 4.472 ± 0.695
3.131ValLys: 3.131 ± 0.398
4.204ValLeu: 4.204 ± 0.49
1.699ValMet: 1.699 ± 0.342
4.293ValAsn: 4.293 ± 0.451
1.521ValPro: 1.521 ± 0.35
2.147ValGln: 2.147 ± 0.446
2.862ValArg: 2.862 ± 0.417
4.83ValSer: 4.83 ± 0.722
4.919ValThr: 4.919 ± 0.812
3.488ValVal: 3.488 ± 0.586
0.626ValTrp: 0.626 ± 0.273
2.147ValTyr: 2.147 ± 0.611
0.0ValXaa: 0.0 ± 0.0
Trp
1.521TrpAla: 1.521 ± 0.339
0.179TrpCys: 0.179 ± 0.135
0.626TrpAsp: 0.626 ± 0.272
0.626TrpGlu: 0.626 ± 0.258
0.716TrpPhe: 0.716 ± 0.271
0.626TrpGly: 0.626 ± 0.215
0.447TrpHis: 0.447 ± 0.208
0.268TrpIle: 0.268 ± 0.151
0.894TrpLys: 0.894 ± 0.256
2.326TrpLeu: 2.326 ± 0.482
0.805TrpMet: 0.805 ± 0.279
0.447TrpAsn: 0.447 ± 0.192
0.716TrpPro: 0.716 ± 0.282
0.716TrpGln: 0.716 ± 0.283
0.716TrpArg: 0.716 ± 0.284
1.073TrpSer: 1.073 ± 0.291
0.626TrpThr: 0.626 ± 0.267
0.984TrpVal: 0.984 ± 0.29
0.179TrpTrp: 0.179 ± 0.121
0.447TrpTyr: 0.447 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.326TyrAla: 2.326 ± 0.512
0.537TyrCys: 0.537 ± 0.227
2.683TyrAsp: 2.683 ± 0.602
1.431TyrGlu: 1.431 ± 0.39
0.805TyrPhe: 0.805 ± 0.278
2.236TyrGly: 2.236 ± 0.661
0.268TyrHis: 0.268 ± 0.2
1.252TyrIle: 1.252 ± 0.331
1.789TyrLys: 1.789 ± 0.362
2.504TyrLeu: 2.504 ± 0.521
0.537TyrMet: 0.537 ± 0.194
2.147TyrAsn: 2.147 ± 0.431
0.805TyrPro: 0.805 ± 0.309
1.878TyrGln: 1.878 ± 0.416
2.683TyrArg: 2.683 ± 0.519
2.147TyrSer: 2.147 ± 0.424
1.163TyrThr: 1.163 ± 0.239
1.699TyrVal: 1.699 ± 0.404
0.447TyrTrp: 0.447 ± 0.202
0.984TyrTyr: 0.984 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski