bacterium 1XD21-70

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8U633|A0A3A8U633_9BACT Riboflavin biosynthesis protein OS=bacterium 1XD21-70 OX=2320103 GN=D7V81_16210 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.14LSKK5 pKa = 10.47SFKK8 pKa = 9.87TILLAAGCVLLLCGCTKK25 pKa = 10.92SNIVRR30 pKa = 11.84TDD32 pKa = 3.14EE33 pKa = 4.08TLGPDD38 pKa = 3.72FNYY41 pKa = 10.78YY42 pKa = 10.52SDD44 pKa = 4.01IEE46 pKa = 3.97IDD48 pKa = 3.44EE49 pKa = 4.74AQLHH53 pKa = 6.97DD54 pKa = 4.84DD55 pKa = 4.02VNDD58 pKa = 3.58IYY60 pKa = 11.2LDD62 pKa = 4.02PNDD65 pKa = 4.31YY66 pKa = 11.21PMASAIDD73 pKa = 4.0FKK75 pKa = 11.53LHH77 pKa = 6.83LDD79 pKa = 3.45EE80 pKa = 6.72AYY82 pKa = 9.68IDD84 pKa = 3.55VDD86 pKa = 4.03VVVKK90 pKa = 10.85DD91 pKa = 3.63GTSPEE96 pKa = 3.92DD97 pKa = 3.53TAWFADD103 pKa = 3.77QAIKK107 pKa = 10.69GINDD111 pKa = 3.37QVCVQDD117 pKa = 4.26FSYY120 pKa = 11.51GEE122 pKa = 4.19SDD124 pKa = 3.99DD125 pKa = 4.01DD126 pKa = 4.22TFGGLYY132 pKa = 9.37QDD134 pKa = 4.6NEE136 pKa = 4.07IHH138 pKa = 6.58LKK140 pKa = 10.69VYY142 pKa = 10.71DD143 pKa = 3.69EE144 pKa = 4.48SSYY147 pKa = 11.91KK148 pKa = 10.7NGTPLYY154 pKa = 6.86EE155 pKa = 4.11TKK157 pKa = 10.19IPKK160 pKa = 9.1DD161 pKa = 3.05AYY163 pKa = 7.98MTFEE167 pKa = 4.04IASS170 pKa = 3.63

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A8UAQ7|A0A3A8UAQ7_9BACT Uncharacterized protein OS=bacterium 1XD21-70 OX=2320103 GN=D7V81_13680 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3860

0

3860

1250839

26

4133

324.1

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.191 ± 0.051

1.563 ± 0.018

5.305 ± 0.03

7.739 ± 0.052

4.024 ± 0.029

8.025 ± 0.052

1.777 ± 0.017

6.476 ± 0.042

6.051 ± 0.032

9.08 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.02

3.837 ± 0.027

3.586 ± 0.024

3.499 ± 0.021

5.301 ± 0.041

5.607 ± 0.03

4.8 ± 0.03

6.944 ± 0.031

1.049 ± 0.015

4.014 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski