Aspergillus tamarii
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13330 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6UQK7|A0A5N6UQK7_9EURO Uncharacterized protein OS=Aspergillus tamarii OX=41984 GN=BDV40DRAFT_201625 PE=4 SV=1
MM1 pKa = 8.28 PDD3 pKa = 3.19 TLSNGEE9 pKa = 4.08 WLDD12 pKa = 3.59 VGDD15 pKa = 5.04 SLWSEE20 pKa = 3.71 NGEE23 pKa = 4.17 FEE25 pKa = 5.97 FRR27 pKa = 11.84 MQQDD31 pKa = 3.08 GKK33 pKa = 10.24 IAVYY37 pKa = 10.35 QGGNPDD43 pKa = 2.95 SGYY46 pKa = 10.83 DD47 pKa = 5.1 GKK49 pKa = 11.06 CLWQNTPEE57 pKa = 3.75 QRR59 pKa = 11.84 YY60 pKa = 9.47 DD61 pKa = 3.15 IKK63 pKa = 10.77 GVKK66 pKa = 7.98 MQEE69 pKa = 4.01 DD70 pKa = 5.1 GNFCMYY76 pKa = 9.88 TDD78 pKa = 4.09 EE79 pKa = 6.47 GIGNCTWHH87 pKa = 6.87 TNTAAPTGDD96 pKa = 3.34 DD97 pKa = 3.19 SVYY100 pKa = 11.17 GIMQNDD106 pKa = 3.93 GNFVLYY112 pKa = 10.34 KK113 pKa = 11.0 SNDD116 pKa = 3.38 QPIWATDD123 pKa = 3.46 TNQQ126 pKa = 2.93
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A5N6UGY9|A0A5N6UGY9_9EURO Non-specific serine/threonine protein kinase OS=Aspergillus tamarii OX=41984 GN=BDV40DRAFT_304698 PE=4 SV=1
MM1 pKa = 7.55 TNLTTTIRR9 pKa = 11.84 PQLPTLSNTQLPSVATPSAQQTRR32 pKa = 11.84 FFSASASLAGKK43 pKa = 9.59 RR44 pKa = 11.84 ATYY47 pKa = 10.03 NPSRR51 pKa = 11.84 RR52 pKa = 11.84 VQKK55 pKa = 10.2 RR56 pKa = 11.84 RR57 pKa = 11.84 HH58 pKa = 5.18 GFLARR63 pKa = 11.84 VRR65 pKa = 11.84 SRR67 pKa = 11.84 GGRR70 pKa = 11.84 MIILRR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 AKK79 pKa = 10.07 GRR81 pKa = 11.84 KK82 pKa = 8.12 SLSWW86 pKa = 3.4
Molecular weight: 9.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.242
IPC_protein 12.661
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.427
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.808
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.149
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13330
0
13330
6167100
49
7779
462.6
51.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.178 ± 0.014
1.38 ± 0.008
5.523 ± 0.013
5.982 ± 0.02
3.874 ± 0.013
6.799 ± 0.02
2.459 ± 0.01
5.214 ± 0.016
4.544 ± 0.015
9.237 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.192 ± 0.008
3.748 ± 0.01
5.843 ± 0.02
4.01 ± 0.013
5.976 ± 0.018
8.285 ± 0.024
5.955 ± 0.013
6.278 ± 0.017
1.538 ± 0.007
2.984 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here