Rickettsia bellii (strain RML369-C)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; belli group; Rickettsia bellii

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1RIU7|Q1RIU7_RICBR Uncharacterized protein OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=RBE_0636 PE=4 SV=1
MM1 pKa = 7.6SNTKK5 pKa = 10.11EE6 pKa = 4.17VVDD9 pKa = 4.79SIEE12 pKa = 5.55DD13 pKa = 3.28ILAFIGDD20 pKa = 4.29AVSDD24 pKa = 3.61MSGGTKK30 pKa = 9.12ATFTLLKK37 pKa = 11.13SMDD40 pKa = 3.74NFNDD44 pKa = 3.1IFSDD48 pKa = 3.61QEE50 pKa = 4.27VIVEE54 pKa = 4.21DD55 pKa = 5.33FYY57 pKa = 11.19PSTYY61 pKa = 10.88SNDD64 pKa = 3.12IFTTAYY70 pKa = 10.46NIIGSVCDD78 pKa = 3.64YY79 pKa = 9.52VCSYY83 pKa = 10.54FWGSKK88 pKa = 8.46

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q1RI79|MRAY_RICBR Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=mraY PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATASGRR28 pKa = 11.84AILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1400

0

1400

424981

40

1981

303.6

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.234 ± 0.054

0.976 ± 0.021

5.126 ± 0.047

6.547 ± 0.072

4.733 ± 0.057

5.328 ± 0.079

1.788 ± 0.03

9.789 ± 0.068

8.735 ± 0.081

10.091 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.03

6.816 ± 0.075

3.282 ± 0.038

3.3 ± 0.037

3.22 ± 0.046

6.807 ± 0.052

5.068 ± 0.057

5.475 ± 0.054

0.761 ± 0.021

3.839 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski