Amino acid dipepetide frequency for Candidatus Burkholderia pumila

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.157AlaAla: 13.157 ± 0.248
1.335AlaCys: 1.335 ± 0.053
6.125AlaAsp: 6.125 ± 0.118
6.036AlaGlu: 6.036 ± 0.13
4.001AlaPhe: 4.001 ± 0.087
8.123AlaGly: 8.123 ± 0.125
2.592AlaHis: 2.592 ± 0.074
5.445AlaIle: 5.445 ± 0.113
4.822AlaLys: 4.822 ± 0.118
12.236AlaLeu: 12.236 ± 0.201
3.177AlaMet: 3.177 ± 0.07
3.487AlaAsn: 3.487 ± 0.09
4.817AlaPro: 4.817 ± 0.121
4.896AlaGln: 4.896 ± 0.111
7.984AlaArg: 7.984 ± 0.154
6.935AlaSer: 6.935 ± 0.13
5.452AlaThr: 5.452 ± 0.098
7.588AlaVal: 7.588 ± 0.133
1.335AlaTrp: 1.335 ± 0.048
2.559AlaTyr: 2.559 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
1.209CysAla: 1.209 ± 0.052
0.161CysCys: 0.161 ± 0.023
0.616CysAsp: 0.616 ± 0.035
0.564CysGlu: 0.564 ± 0.033
0.388CysPhe: 0.388 ± 0.03
1.109CysGly: 1.109 ± 0.049
0.275CysHis: 0.275 ± 0.027
0.521CysIle: 0.521 ± 0.032
0.333CysLys: 0.333 ± 0.026
0.861CysLeu: 0.861 ± 0.045
0.221CysMet: 0.221 ± 0.021
0.3CysAsn: 0.3 ± 0.023
0.56CysPro: 0.56 ± 0.04
0.254CysGln: 0.254 ± 0.024
0.7CysArg: 0.7 ± 0.037
0.613CysSer: 0.613 ± 0.037
0.525CysThr: 0.525 ± 0.037
0.793CysVal: 0.793 ± 0.041
0.083CysTrp: 0.083 ± 0.015
0.267CysTyr: 0.267 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
7.519AspAla: 7.519 ± 0.145
0.576AspCys: 0.576 ± 0.034
3.377AspAsp: 3.377 ± 0.088
3.979AspGlu: 3.979 ± 0.097
2.148AspPhe: 2.148 ± 0.069
4.324AspGly: 4.324 ± 0.105
1.223AspHis: 1.223 ± 0.052
3.123AspIle: 3.123 ± 0.08
2.338AspLys: 2.338 ± 0.074
5.241AspLeu: 5.241 ± 0.113
1.295AspMet: 1.295 ± 0.043
1.593AspAsn: 1.593 ± 0.062
2.719AspPro: 2.719 ± 0.071
1.735AspGln: 1.735 ± 0.06
3.311AspArg: 3.311 ± 0.078
2.793AspSer: 2.793 ± 0.081
2.838AspThr: 2.838 ± 0.071
4.507AspVal: 4.507 ± 0.109
0.886AspTrp: 0.886 ± 0.041
1.615AspTyr: 1.615 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
6.71GluAla: 6.71 ± 0.132
0.521GluCys: 0.521 ± 0.034
2.58GluAsp: 2.58 ± 0.082
3.057GluGlu: 3.057 ± 0.105
2.115GluPhe: 2.115 ± 0.072
3.656GluGly: 3.656 ± 0.087
1.514GluHis: 1.514 ± 0.051
3.483GluIle: 3.483 ± 0.098
2.648GluLys: 2.648 ± 0.084
5.615GluLeu: 5.615 ± 0.123
1.593GluMet: 1.593 ± 0.053
1.822GluAsn: 1.822 ± 0.047
2.442GluPro: 2.442 ± 0.066
2.535GluGln: 2.535 ± 0.068
4.799GluArg: 4.799 ± 0.105
3.057GluSer: 3.057 ± 0.065
3.272GluThr: 3.272 ± 0.093
4.067GluVal: 4.067 ± 0.114
0.752GluTrp: 0.752 ± 0.039
1.32GluTyr: 1.32 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
4.239PheAla: 4.239 ± 0.096
0.409PheCys: 0.409 ± 0.027
2.909PheAsp: 2.909 ± 0.078
2.299PheGlu: 2.299 ± 0.066
1.572PhePhe: 1.572 ± 0.062
3.475PheGly: 3.475 ± 0.098
0.855PheHis: 0.855 ± 0.044
1.971PheIle: 1.971 ± 0.062
1.367PheLys: 1.367 ± 0.05
2.898PheLeu: 2.898 ± 0.073
0.927PheMet: 0.927 ± 0.045
1.242PheAsn: 1.242 ± 0.049
1.574PhePro: 1.574 ± 0.06
1.039PheGln: 1.039 ± 0.05
2.064PheArg: 2.064 ± 0.053
2.367PheSer: 2.367 ± 0.064
2.093PheThr: 2.093 ± 0.063
3.026PheVal: 3.026 ± 0.084
0.487PheTrp: 0.487 ± 0.031
0.994PheTyr: 0.994 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
7.837GlyAla: 7.837 ± 0.133
0.82GlyCys: 0.82 ± 0.041
3.946GlyAsp: 3.946 ± 0.098
4.396GlyGlu: 4.396 ± 0.097
3.225GlyPhe: 3.225 ± 0.091
5.958GlyGly: 5.958 ± 0.146
1.911GlyHis: 1.911 ± 0.063
4.344GlyIle: 4.344 ± 0.089
3.884GlyLys: 3.884 ± 0.09
7.057GlyLeu: 7.057 ± 0.128
2.204GlyMet: 2.204 ± 0.063
2.394GlyAsn: 2.394 ± 0.069
2.45GlyPro: 2.45 ± 0.075
2.553GlyGln: 2.553 ± 0.068
4.875GlyArg: 4.875 ± 0.099
4.328GlySer: 4.328 ± 0.093
4.165GlyThr: 4.165 ± 0.085
6.063GlyVal: 6.063 ± 0.105
1.128GlyTrp: 1.128 ± 0.056
2.093GlyTyr: 2.093 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
2.871HisAla: 2.871 ± 0.081
0.298HisCys: 0.298 ± 0.022
1.386HisAsp: 1.386 ± 0.054
1.357HisGlu: 1.357 ± 0.051
0.971HisPhe: 0.971 ± 0.041
2.13HisGly: 2.13 ± 0.057
0.642HisHis: 0.642 ± 0.038
1.186HisIle: 1.186 ± 0.053
0.758HisLys: 0.758 ± 0.038
2.07HisLeu: 2.07 ± 0.06
0.529HisMet: 0.529 ± 0.033
0.686HisAsn: 0.686 ± 0.04
1.394HisPro: 1.394 ± 0.058
0.723HisGln: 0.723 ± 0.037
1.593HisArg: 1.593 ± 0.055
1.116HisSer: 1.116 ± 0.043
1.229HisThr: 1.229 ± 0.055
1.774HisVal: 1.774 ± 0.055
0.331HisTrp: 0.331 ± 0.024
0.713HisTyr: 0.713 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.962IleAla: 6.962 ± 0.12
0.597IleCys: 0.597 ± 0.035
4.191IleAsp: 4.191 ± 0.108
4.119IleGlu: 4.119 ± 0.097
1.727IlePhe: 1.727 ± 0.063
4.97IleGly: 4.97 ± 0.119
1.118IleHis: 1.118 ± 0.046
2.328IleIle: 2.328 ± 0.076
2.004IleLys: 2.004 ± 0.068
3.795IleLeu: 3.795 ± 0.104
1.012IleMet: 1.012 ± 0.054
1.791IleAsn: 1.791 ± 0.063
2.206IlePro: 2.206 ± 0.063
1.374IleGln: 1.374 ± 0.057
3.008IleArg: 3.008 ± 0.079
2.78IleSer: 2.78 ± 0.077
2.619IleThr: 2.619 ± 0.076
4.729IleVal: 4.729 ± 0.109
0.535IleTrp: 0.535 ± 0.033
1.194IleTyr: 1.194 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.222LysAla: 4.222 ± 0.102
0.3LysCys: 0.3 ± 0.023
2.101LysAsp: 2.101 ± 0.066
2.109LysGlu: 2.109 ± 0.077
1.38LysPhe: 1.38 ± 0.049
2.745LysGly: 2.745 ± 0.076
0.932LysHis: 0.932 ± 0.044
2.305LysIle: 2.305 ± 0.071
2.138LysLys: 2.138 ± 0.081
4.462LysLeu: 4.462 ± 0.1
1.208LysMet: 1.208 ± 0.051
1.37LysAsn: 1.37 ± 0.055
2.512LysPro: 2.512 ± 0.079
1.754LysGln: 1.754 ± 0.066
3.136LysArg: 3.136 ± 0.075
2.497LysSer: 2.497 ± 0.079
2.469LysThr: 2.469 ± 0.08
2.873LysVal: 2.873 ± 0.097
0.463LysTrp: 0.463 ± 0.031
0.981LysTyr: 0.981 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
11.446LeuAla: 11.446 ± 0.165
1.012LeuCys: 1.012 ± 0.046
6.028LeuAsp: 6.028 ± 0.117
5.191LeuGlu: 5.191 ± 0.11
3.423LeuPhe: 3.423 ± 0.091
7.154LeuGly: 7.154 ± 0.125
2.332LeuHis: 2.332 ± 0.074
5.051LeuIle: 5.051 ± 0.101
4.251LeuLys: 4.251 ± 0.106
8.932LeuLeu: 8.932 ± 0.176
2.446LeuMet: 2.446 ± 0.072
3.161LeuAsn: 3.161 ± 0.082
5.255LeuPro: 5.255 ± 0.101
3.28LeuGln: 3.28 ± 0.082
6.627LeuArg: 6.627 ± 0.121
6.168LeuSer: 6.168 ± 0.123
5.394LeuThr: 5.394 ± 0.108
6.91LeuVal: 6.91 ± 0.139
0.925LeuTrp: 0.925 ± 0.04
2.161LeuTyr: 2.161 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.498MetAla: 2.498 ± 0.067
0.219MetCys: 0.219 ± 0.022
1.064MetAsp: 1.064 ± 0.049
1.138MetGlu: 1.138 ± 0.046
0.866MetPhe: 0.866 ± 0.047
1.547MetGly: 1.547 ± 0.057
0.68MetHis: 0.68 ± 0.03
1.229MetIle: 1.229 ± 0.053
1.316MetLys: 1.316 ± 0.046
2.956MetLeu: 2.956 ± 0.083
0.713MetMet: 0.713 ± 0.041
1.132MetAsn: 1.132 ± 0.039
1.615MetPro: 1.615 ± 0.055
1.256MetGln: 1.256 ± 0.05
2.078MetArg: 2.078 ± 0.065
1.81MetSer: 1.81 ± 0.052
1.69MetThr: 1.69 ± 0.062
1.576MetVal: 1.576 ± 0.055
0.225MetTrp: 0.225 ± 0.024
0.481MetTyr: 0.481 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.811AsnAla: 3.811 ± 0.078
0.368AsnCys: 0.368 ± 0.031
1.88AsnAsp: 1.88 ± 0.066
1.787AsnGlu: 1.787 ± 0.055
1.258AsnPhe: 1.258 ± 0.051
2.778AsnGly: 2.778 ± 0.079
0.665AsnHis: 0.665 ± 0.036
1.686AsnIle: 1.686 ± 0.062
1.165AsnLys: 1.165 ± 0.044
3.092AsnLeu: 3.092 ± 0.082
0.752AsnMet: 0.752 ± 0.038
1.004AsnAsn: 1.004 ± 0.046
1.931AsnPro: 1.931 ± 0.063
1.018AsnGln: 1.018 ± 0.044
1.975AsnArg: 1.975 ± 0.067
1.525AsnSer: 1.525 ± 0.058
1.663AsnThr: 1.663 ± 0.057
2.495AsnVal: 2.495 ± 0.08
0.471AsnTrp: 0.471 ± 0.031
0.897AsnTyr: 0.897 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
5.175ProAla: 5.175 ± 0.125
0.419ProCys: 0.419 ± 0.028
3.109ProAsp: 3.109 ± 0.083
3.152ProGlu: 3.152 ± 0.082
1.894ProPhe: 1.894 ± 0.063
3.464ProGly: 3.464 ± 0.077
1.254ProHis: 1.254 ± 0.052
2.341ProIle: 2.341 ± 0.066
1.826ProLys: 1.826 ± 0.066
4.522ProLeu: 4.522 ± 0.09
1.184ProMet: 1.184 ± 0.059
1.704ProAsn: 1.704 ± 0.059
2.144ProPro: 2.144 ± 0.074
1.861ProGln: 1.861 ± 0.068
2.673ProArg: 2.673 ± 0.075
2.878ProSer: 2.878 ± 0.077
2.423ProThr: 2.423 ± 0.071
4.115ProVal: 4.115 ± 0.091
0.537ProTrp: 0.537 ± 0.028
1.208ProTyr: 1.208 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.113
0.333GlnCys: 0.333 ± 0.026
1.518GlnAsp: 1.518 ± 0.052
1.743GlnGlu: 1.743 ± 0.065
1.324GlnPhe: 1.324 ± 0.056
2.38GlnGly: 2.38 ± 0.076
0.932GlnHis: 0.932 ± 0.04
2.219GlnIle: 2.219 ± 0.062
1.584GlnLys: 1.584 ± 0.049
3.489GlnLeu: 3.489 ± 0.085
1.08GlnMet: 1.08 ± 0.049
1.115GlnAsn: 1.115 ± 0.051
1.774GlnPro: 1.774 ± 0.062
1.739GlnGln: 1.739 ± 0.068
2.739GlnArg: 2.739 ± 0.077
2.07GlnSer: 2.07 ± 0.062
2.035GlnThr: 2.035 ± 0.064
2.553GlnVal: 2.553 ± 0.079
0.492GlnTrp: 0.492 ± 0.031
1.021GlnTyr: 1.021 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
7.307ArgAla: 7.307 ± 0.144
0.702ArgCys: 0.702 ± 0.042
3.708ArgAsp: 3.708 ± 0.073
4.495ArgGlu: 4.495 ± 0.094
2.925ArgPhe: 2.925 ± 0.075
4.563ArgGly: 4.563 ± 0.105
1.805ArgHis: 1.805 ± 0.064
3.871ArgIle: 3.871 ± 0.085
2.597ArgLys: 2.597 ± 0.077
6.602ArgLeu: 6.602 ± 0.145
2.045ArgMet: 2.045 ± 0.065
2.235ArgAsn: 2.235 ± 0.064
2.785ArgPro: 2.785 ± 0.072
2.371ArgGln: 2.371 ± 0.073
5.15ArgArg: 5.15 ± 0.115
3.543ArgSer: 3.543 ± 0.092
3.272ArgThr: 3.272 ± 0.085
5.007ArgVal: 5.007 ± 0.092
0.919ArgTrp: 0.919 ± 0.039
1.942ArgTyr: 1.942 ± 0.066
0.0ArgXaa: 0.0 ± 0.0
Ser
6.222SerAla: 6.222 ± 0.126
0.568SerCys: 0.568 ± 0.038
3.169SerAsp: 3.169 ± 0.079
2.987SerGlu: 2.987 ± 0.074
2.289SerPhe: 2.289 ± 0.075
5.001SerGly: 5.001 ± 0.104
1.287SerHis: 1.287 ± 0.044
3.125SerIle: 3.125 ± 0.077
2.347SerLys: 2.347 ± 0.068
5.569SerLeu: 5.569 ± 0.101
1.545SerMet: 1.545 ± 0.055
1.913SerAsn: 1.913 ± 0.068
2.702SerPro: 2.702 ± 0.075
1.931SerGln: 1.931 ± 0.066
3.834SerArg: 3.834 ± 0.096
3.619SerSer: 3.619 ± 0.11
3.208SerThr: 3.208 ± 0.092
4.282SerVal: 4.282 ± 0.09
0.669SerTrp: 0.669 ± 0.036
1.388SerTyr: 1.388 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.293ThrAla: 5.293 ± 0.099
0.529ThrCys: 0.529 ± 0.032
2.96ThrAsp: 2.96 ± 0.08
2.708ThrGlu: 2.708 ± 0.072
1.979ThrPhe: 1.979 ± 0.063
4.311ThrGly: 4.311 ± 0.086
1.244ThrHis: 1.244 ± 0.051
2.776ThrIle: 2.776 ± 0.076
1.886ThrLys: 1.886 ± 0.051
6.131ThrLeu: 6.131 ± 0.114
1.295ThrMet: 1.295 ± 0.051
1.64ThrAsn: 1.64 ± 0.063
3.324ThrPro: 3.324 ± 0.082
1.905ThrGln: 1.905 ± 0.065
3.524ThrArg: 3.524 ± 0.082
3.072ThrSer: 3.072 ± 0.075
2.906ThrThr: 2.906 ± 0.086
4.198ThrVal: 4.198 ± 0.093
0.535ThrTrp: 0.535 ± 0.035
1.246ThrTyr: 1.246 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
7.99ValAla: 7.99 ± 0.14
0.787ValCys: 0.787 ± 0.044
4.417ValAsp: 4.417 ± 0.107
4.508ValGlu: 4.508 ± 0.093
2.716ValPhe: 2.716 ± 0.092
5.073ValGly: 5.073 ± 0.107
1.58ValHis: 1.58 ± 0.052
4.224ValIle: 4.224 ± 0.094
3.256ValLys: 3.256 ± 0.092
7.482ValLeu: 7.482 ± 0.128
1.921ValMet: 1.921 ± 0.062
2.44ValAsn: 2.44 ± 0.075
3.888ValPro: 3.888 ± 0.093
2.613ValGln: 2.613 ± 0.075
4.912ValArg: 4.912 ± 0.097
4.536ValSer: 4.536 ± 0.106
4.377ValThr: 4.377 ± 0.087
6.084ValVal: 6.084 ± 0.116
0.845ValTrp: 0.845 ± 0.041
1.632ValTyr: 1.632 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.89TrpAla: 0.89 ± 0.044
0.132TrpCys: 0.132 ± 0.019
0.568TrpAsp: 0.568 ± 0.031
0.516TrpGlu: 0.516 ± 0.036
0.508TrpPhe: 0.508 ± 0.034
0.729TrpGly: 0.729 ± 0.037
0.372TrpHis: 0.372 ± 0.03
0.758TrpIle: 0.758 ± 0.039
0.498TrpLys: 0.498 ± 0.029
1.675TrpLeu: 1.675 ± 0.053
0.366TrpMet: 0.366 ± 0.027
0.442TrpAsn: 0.442 ± 0.03
0.624TrpPro: 0.624 ± 0.037
0.564TrpGln: 0.564 ± 0.033
1.006TrpArg: 1.006 ± 0.053
0.655TrpSer: 0.655 ± 0.034
0.599TrpThr: 0.599 ± 0.038
0.814TrpVal: 0.814 ± 0.038
0.161TrpTrp: 0.161 ± 0.017
0.266TrpTyr: 0.266 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.607TyrAla: 2.607 ± 0.075
0.267TyrCys: 0.267 ± 0.024
1.487TyrAsp: 1.487 ± 0.061
1.419TyrGlu: 1.419 ± 0.055
1.099TyrPhe: 1.099 ± 0.049
2.144TyrGly: 2.144 ± 0.073
0.545TyrHis: 0.545 ± 0.03
1.074TyrIle: 1.074 ± 0.049
0.938TyrLys: 0.938 ± 0.046
2.462TyrLeu: 2.462 ± 0.074
0.576TyrMet: 0.576 ± 0.035
0.748TyrAsn: 0.748 ± 0.039
1.167TyrPro: 1.167 ± 0.047
0.878TyrGln: 0.878 ± 0.045
1.855TyrArg: 1.855 ± 0.063
1.291TyrSer: 1.291 ± 0.046
1.233TyrThr: 1.233 ± 0.049
1.828TyrVal: 1.828 ± 0.059
0.362TyrTrp: 0.362 ± 0.027
0.663TyrTyr: 0.663 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1736 proteins (515917 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski