Lactococcus phage 63302

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus 63302

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343JQ79|A0A343JQ79_9CAUD Structural protein 4 OS=Lactococcus phage 63302 OX=2029670 GN=63302_10 PE=4 SV=1
MM1 pKa = 7.52EE2 pKa = 5.73FDD4 pKa = 6.24SYY6 pKa = 11.4MDD8 pKa = 3.4WLNNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 9.05DD27 pKa = 3.82TIQDD31 pKa = 3.37KK32 pKa = 8.92TVYY35 pKa = 10.59LSLSDD40 pKa = 3.67SKK42 pKa = 11.01VIKK45 pKa = 9.75MDD47 pKa = 3.15NTSFIMGYY55 pKa = 9.09YY56 pKa = 7.66YY57 pKa = 10.47QVVLSVKK64 pKa = 10.52DD65 pKa = 3.51VDD67 pKa = 4.41DD68 pKa = 4.44EE69 pKa = 4.52LVGLVGDD76 pKa = 4.15VLQNGWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.42LPCGAGGQAWQQ112 pKa = 3.59

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343JQ97|A0A343JQ97_9CAUD Uncharacterized protein OS=Lactococcus phage 63302 OX=2029670 GN=63302_28 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.58SIATAQHH21 pKa = 5.37IKK23 pKa = 10.55KK24 pKa = 10.01NKK26 pKa = 8.71KK27 pKa = 9.41NKK29 pKa = 8.96NDD31 pKa = 4.3KK32 pKa = 10.52IPVRR36 pKa = 11.84AYY38 pKa = 8.85YY39 pKa = 10.06CKK41 pKa = 9.74WCNSYY46 pKa = 10.56HH47 pKa = 7.02LSSQQRR53 pKa = 11.84LNIKK57 pKa = 9.21TGVIGG62 pKa = 3.97

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

9374

42

999

187.5

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.198 ± 0.519

0.693 ± 0.153

5.654 ± 0.308

7.318 ± 0.585

4.086 ± 0.336

6.145 ± 0.614

1.227 ± 0.164

6.763 ± 0.318

9.078 ± 0.509

8.577 ± 0.369

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.182

6.998 ± 0.34

2.283 ± 0.235

3.734 ± 0.22

3.456 ± 0.245

6.497 ± 0.468

6.763 ± 0.361

6.401 ± 0.372

1.44 ± 0.161

4.31 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski