Amino acid dipepetide frequency for Lactococcus phage 63302

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.854AlaAla: 0.854 ± 0.45
0.107AlaCys: 0.107 ± 0.116
3.094AlaAsp: 3.094 ± 0.596
4.374AlaGlu: 4.374 ± 0.658
3.307AlaPhe: 3.307 ± 0.661
3.841AlaGly: 3.841 ± 0.775
0.747AlaHis: 0.747 ± 0.313
5.228AlaIle: 5.228 ± 0.963
6.295AlaLys: 6.295 ± 0.891
5.868AlaLeu: 5.868 ± 0.851
2.134AlaMet: 2.134 ± 0.546
5.014AlaAsn: 5.014 ± 1.133
1.707AlaPro: 1.707 ± 0.423
2.347AlaGln: 2.347 ± 0.558
2.454AlaArg: 2.454 ± 0.49
3.201AlaSer: 3.201 ± 0.666
3.734AlaThr: 3.734 ± 0.896
3.734AlaVal: 3.734 ± 0.801
1.707AlaTrp: 1.707 ± 0.619
2.24AlaTyr: 2.24 ± 0.436
0.0AlaXaa: 0.0 ± 0.0
Cys
0.213CysAla: 0.213 ± 0.151
0.0CysCys: 0.0 ± 0.0
0.213CysAsp: 0.213 ± 0.148
0.32CysGlu: 0.32 ± 0.216
0.213CysPhe: 0.213 ± 0.147
0.854CysGly: 0.854 ± 0.356
0.213CysHis: 0.213 ± 0.138
0.32CysIle: 0.32 ± 0.181
0.96CysLys: 0.96 ± 0.407
0.213CysLeu: 0.213 ± 0.155
0.0CysMet: 0.0 ± 0.0
0.427CysAsn: 0.427 ± 0.211
0.213CysPro: 0.213 ± 0.148
0.32CysGln: 0.32 ± 0.151
0.533CysArg: 0.533 ± 0.241
0.32CysSer: 0.32 ± 0.173
0.32CysThr: 0.32 ± 0.175
0.64CysVal: 0.64 ± 0.318
0.107CysTrp: 0.107 ± 0.109
0.533CysTyr: 0.533 ± 0.293
0.0CysXaa: 0.0 ± 0.0
Asp
2.24AspAla: 2.24 ± 0.622
0.107AspCys: 0.107 ± 0.105
3.627AspAsp: 3.627 ± 0.825
3.948AspGlu: 3.948 ± 0.746
3.521AspPhe: 3.521 ± 0.594
3.734AspGly: 3.734 ± 0.652
0.747AspHis: 0.747 ± 0.363
3.307AspIle: 3.307 ± 0.638
5.014AspLys: 5.014 ± 0.609
6.615AspLeu: 6.615 ± 0.956
0.96AspMet: 0.96 ± 0.258
4.481AspAsn: 4.481 ± 0.73
1.494AspPro: 1.494 ± 0.487
0.533AspGln: 0.533 ± 0.275
1.6AspArg: 1.6 ± 0.455
2.667AspSer: 2.667 ± 0.491
4.374AspThr: 4.374 ± 0.673
3.841AspVal: 3.841 ± 0.669
0.96AspTrp: 0.96 ± 0.287
2.774AspTyr: 2.774 ± 0.476
0.0AspXaa: 0.0 ± 0.0
Glu
3.841GluAla: 3.841 ± 0.521
0.747GluCys: 0.747 ± 0.334
2.774GluAsp: 2.774 ± 0.492
5.228GluGlu: 5.228 ± 0.829
4.161GluPhe: 4.161 ± 0.554
2.134GluGly: 2.134 ± 0.424
0.747GluHis: 0.747 ± 0.305
5.334GluIle: 5.334 ± 0.862
6.081GluLys: 6.081 ± 1.231
9.602GluLeu: 9.602 ± 1.335
2.24GluMet: 2.24 ± 0.471
5.014GluAsn: 5.014 ± 0.677
1.814GluPro: 1.814 ± 0.41
3.521GluGln: 3.521 ± 0.701
2.881GluArg: 2.881 ± 0.583
4.054GluSer: 4.054 ± 0.561
4.801GluThr: 4.801 ± 0.74
3.841GluVal: 3.841 ± 0.569
1.067GluTrp: 1.067 ± 0.305
3.307GluTyr: 3.307 ± 0.696
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 0.607
0.32PheCys: 0.32 ± 0.197
3.094PheAsp: 3.094 ± 0.491
2.667PheGlu: 2.667 ± 0.5
2.027PhePhe: 2.027 ± 0.672
2.774PheGly: 2.774 ± 0.549
0.533PheHis: 0.533 ± 0.228
3.201PheIle: 3.201 ± 0.535
3.841PheLys: 3.841 ± 0.518
2.24PheLeu: 2.24 ± 0.511
0.854PheMet: 0.854 ± 0.316
3.307PheAsn: 3.307 ± 0.866
0.64PhePro: 0.64 ± 0.23
0.96PheGln: 0.96 ± 0.28
1.28PheArg: 1.28 ± 0.343
3.948PheSer: 3.948 ± 0.77
2.561PheThr: 2.561 ± 0.451
2.454PheVal: 2.454 ± 0.429
0.32PheTrp: 0.32 ± 0.164
1.387PheTyr: 1.387 ± 0.357
0.0PheXaa: 0.0 ± 0.0
Gly
3.201GlyAla: 3.201 ± 0.898
0.427GlyCys: 0.427 ± 0.207
3.734GlyAsp: 3.734 ± 0.596
3.734GlyGlu: 3.734 ± 0.625
1.92GlyPhe: 1.92 ± 0.659
3.948GlyGly: 3.948 ± 0.723
0.747GlyHis: 0.747 ± 0.247
3.627GlyIle: 3.627 ± 1.374
6.401GlyLys: 6.401 ± 0.726
6.081GlyLeu: 6.081 ± 1.066
0.96GlyMet: 0.96 ± 0.318
3.521GlyAsn: 3.521 ± 0.54
0.0GlyPro: 0.0 ± 0.0
1.6GlyGln: 1.6 ± 0.396
2.24GlyArg: 2.24 ± 0.393
4.694GlySer: 4.694 ± 0.803
4.268GlyThr: 4.268 ± 0.983
5.761GlyVal: 5.761 ± 0.95
0.96GlyTrp: 0.96 ± 0.325
3.627GlyTyr: 3.627 ± 0.587
0.0GlyXaa: 0.0 ± 0.0
His
1.174HisAla: 1.174 ± 0.367
0.32HisCys: 0.32 ± 0.197
0.533HisAsp: 0.533 ± 0.313
0.64HisGlu: 0.64 ± 0.255
0.747HisPhe: 0.747 ± 0.295
1.174HisGly: 1.174 ± 0.352
0.213HisHis: 0.213 ± 0.154
1.174HisIle: 1.174 ± 0.329
0.533HisLys: 0.533 ± 0.239
0.747HisLeu: 0.747 ± 0.327
0.32HisMet: 0.32 ± 0.24
1.28HisAsn: 1.28 ± 0.45
0.213HisPro: 0.213 ± 0.149
0.213HisGln: 0.213 ± 0.143
0.213HisArg: 0.213 ± 0.169
0.533HisSer: 0.533 ± 0.211
1.067HisThr: 1.067 ± 0.381
0.533HisVal: 0.533 ± 0.255
0.0HisTrp: 0.0 ± 0.0
0.64HisTyr: 0.64 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
3.841IleAla: 3.841 ± 0.55
0.213IleCys: 0.213 ± 0.153
3.948IleAsp: 3.948 ± 0.545
5.868IleGlu: 5.868 ± 1.058
2.454IlePhe: 2.454 ± 0.553
3.841IleGly: 3.841 ± 0.818
0.747IleHis: 0.747 ± 0.297
4.268IleIle: 4.268 ± 0.606
6.721IleLys: 6.721 ± 0.748
5.014IleLeu: 5.014 ± 0.734
1.707IleMet: 1.707 ± 0.476
5.228IleAsn: 5.228 ± 0.664
1.707IlePro: 1.707 ± 0.398
1.92IleGln: 1.92 ± 0.38
2.134IleArg: 2.134 ± 0.462
3.948IleSer: 3.948 ± 0.792
4.801IleThr: 4.801 ± 0.608
4.588IleVal: 4.588 ± 0.616
1.387IleTrp: 1.387 ± 0.396
3.307IleTyr: 3.307 ± 0.559
0.0IleXaa: 0.0 ± 0.0
Lys
7.788LysAla: 7.788 ± 1.084
0.64LysCys: 0.64 ± 0.315
4.588LysAsp: 4.588 ± 0.738
8.108LysGlu: 8.108 ± 1.284
2.134LysPhe: 2.134 ± 0.49
5.228LysGly: 5.228 ± 0.878
1.387LysHis: 1.387 ± 0.48
5.975LysIle: 5.975 ± 0.944
9.069LysLys: 9.069 ± 1.241
7.575LysLeu: 7.575 ± 0.671
2.987LysMet: 2.987 ± 0.439
5.014LysAsn: 5.014 ± 0.694
1.28LysPro: 1.28 ± 0.399
3.521LysGln: 3.521 ± 0.707
3.841LysArg: 3.841 ± 0.609
5.548LysSer: 5.548 ± 0.969
5.121LysThr: 5.121 ± 0.786
6.081LysVal: 6.081 ± 0.732
1.067LysTrp: 1.067 ± 0.285
3.841LysTyr: 3.841 ± 0.766
0.0LysXaa: 0.0 ± 0.0
Leu
4.801LeuAla: 4.801 ± 0.56
0.32LeuCys: 0.32 ± 0.165
5.014LeuAsp: 5.014 ± 0.724
6.081LeuGlu: 6.081 ± 0.972
3.094LeuPhe: 3.094 ± 0.607
4.054LeuGly: 4.054 ± 0.556
1.387LeuHis: 1.387 ± 0.419
6.828LeuIle: 6.828 ± 0.927
8.108LeuLys: 8.108 ± 0.73
6.401LeuLeu: 6.401 ± 0.949
1.814LeuMet: 1.814 ± 0.563
5.548LeuAsn: 5.548 ± 0.903
2.881LeuPro: 2.881 ± 0.492
3.734LeuGln: 3.734 ± 0.653
2.774LeuArg: 2.774 ± 0.566
5.121LeuSer: 5.121 ± 0.794
6.295LeuThr: 6.295 ± 0.772
5.868LeuVal: 5.868 ± 0.608
1.387LeuTrp: 1.387 ± 0.389
4.268LeuTyr: 4.268 ± 0.742
0.0LeuXaa: 0.0 ± 0.0
Met
2.134MetAla: 2.134 ± 0.469
0.107MetCys: 0.107 ± 0.103
1.387MetAsp: 1.387 ± 0.544
1.814MetGlu: 1.814 ± 0.518
0.427MetPhe: 0.427 ± 0.211
0.64MetGly: 0.64 ± 0.233
0.32MetHis: 0.32 ± 0.195
1.814MetIle: 1.814 ± 0.385
2.134MetLys: 2.134 ± 0.428
1.92MetLeu: 1.92 ± 0.655
0.32MetMet: 0.32 ± 0.165
1.92MetAsn: 1.92 ± 0.42
0.533MetPro: 0.533 ± 0.234
1.494MetGln: 1.494 ± 0.35
0.213MetArg: 0.213 ± 0.159
1.707MetSer: 1.707 ± 0.408
1.814MetThr: 1.814 ± 0.543
1.6MetVal: 1.6 ± 0.316
0.107MetTrp: 0.107 ± 0.101
1.387MetTyr: 1.387 ± 0.383
0.0MetXaa: 0.0 ± 0.0
Asn
5.228AsnAla: 5.228 ± 1.262
0.32AsnCys: 0.32 ± 0.18
4.694AsnAsp: 4.694 ± 0.857
4.588AsnGlu: 4.588 ± 0.694
1.707AsnPhe: 1.707 ± 0.458
7.042AsnGly: 7.042 ± 0.83
0.747AsnHis: 0.747 ± 0.264
4.374AsnIle: 4.374 ± 0.599
6.935AsnLys: 6.935 ± 1.318
6.401AsnLeu: 6.401 ± 1.122
1.387AsnMet: 1.387 ± 0.381
3.841AsnAsn: 3.841 ± 0.664
1.92AsnPro: 1.92 ± 0.424
2.881AsnGln: 2.881 ± 0.584
1.92AsnArg: 1.92 ± 0.436
5.228AsnSer: 5.228 ± 0.868
4.054AsnThr: 4.054 ± 0.688
2.987AsnVal: 2.987 ± 0.522
1.28AsnTrp: 1.28 ± 0.374
2.347AsnTyr: 2.347 ± 0.563
0.0AsnXaa: 0.0 ± 0.0
Pro
1.067ProAla: 1.067 ± 0.466
0.107ProCys: 0.107 ± 0.121
1.174ProAsp: 1.174 ± 0.342
1.814ProGlu: 1.814 ± 0.452
0.96ProPhe: 0.96 ± 0.354
0.32ProGly: 0.32 ± 0.241
0.0ProHis: 0.0 ± 0.0
1.6ProIle: 1.6 ± 0.54
2.134ProLys: 2.134 ± 0.479
1.92ProLeu: 1.92 ± 0.395
0.64ProMet: 0.64 ± 0.262
2.347ProAsn: 2.347 ± 0.71
0.854ProPro: 0.854 ± 0.305
0.96ProGln: 0.96 ± 0.351
0.64ProArg: 0.64 ± 0.247
0.96ProSer: 0.96 ± 0.378
2.24ProThr: 2.24 ± 0.421
2.027ProVal: 2.027 ± 0.503
0.107ProTrp: 0.107 ± 0.108
0.96ProTyr: 0.96 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
3.734GlnAla: 3.734 ± 0.76
0.107GlnCys: 0.107 ± 0.111
2.027GlnAsp: 2.027 ± 0.596
2.134GlnGlu: 2.134 ± 0.528
1.494GlnPhe: 1.494 ± 0.386
2.454GlnGly: 2.454 ± 0.475
0.32GlnHis: 0.32 ± 0.189
1.387GlnIle: 1.387 ± 0.309
3.094GlnLys: 3.094 ± 0.539
3.201GlnLeu: 3.201 ± 0.677
0.747GlnMet: 0.747 ± 0.258
2.454GlnAsn: 2.454 ± 0.439
1.28GlnPro: 1.28 ± 0.358
1.92GlnGln: 1.92 ± 0.544
1.707GlnArg: 1.707 ± 0.471
2.454GlnSer: 2.454 ± 0.576
2.987GlnThr: 2.987 ± 0.456
2.134GlnVal: 2.134 ± 0.443
0.854GlnTrp: 0.854 ± 0.301
0.854GlnTyr: 0.854 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
2.347ArgAla: 2.347 ± 0.615
0.427ArgCys: 0.427 ± 0.19
1.6ArgAsp: 1.6 ± 0.401
2.454ArgGlu: 2.454 ± 0.443
0.854ArgPhe: 0.854 ± 0.292
2.24ArgGly: 2.24 ± 0.471
0.64ArgHis: 0.64 ± 0.297
2.24ArgIle: 2.24 ± 0.438
3.627ArgLys: 3.627 ± 0.826
3.521ArgLeu: 3.521 ± 0.508
0.64ArgMet: 0.64 ± 0.321
2.561ArgAsn: 2.561 ± 0.632
0.747ArgPro: 0.747 ± 0.292
1.387ArgGln: 1.387 ± 0.407
1.494ArgArg: 1.494 ± 0.414
2.347ArgSer: 2.347 ± 0.354
2.027ArgThr: 2.027 ± 0.347
1.387ArgVal: 1.387 ± 0.39
0.32ArgTrp: 0.32 ± 0.186
1.707ArgTyr: 1.707 ± 0.437
0.0ArgXaa: 0.0 ± 0.0
Ser
4.374SerAla: 4.374 ± 0.983
0.64SerCys: 0.64 ± 0.302
3.627SerAsp: 3.627 ± 0.564
4.694SerGlu: 4.694 ± 0.836
2.987SerPhe: 2.987 ± 0.513
5.761SerGly: 5.761 ± 1.666
0.747SerHis: 0.747 ± 0.255
3.627SerIle: 3.627 ± 0.661
5.441SerLys: 5.441 ± 0.846
5.441SerLeu: 5.441 ± 0.703
1.814SerMet: 1.814 ± 0.348
3.734SerAsn: 3.734 ± 0.885
1.174SerPro: 1.174 ± 0.356
2.561SerGln: 2.561 ± 0.562
2.347SerArg: 2.347 ± 0.409
5.228SerSer: 5.228 ± 0.714
3.521SerThr: 3.521 ± 0.827
4.268SerVal: 4.268 ± 0.747
0.96SerTrp: 0.96 ± 0.328
2.027SerTyr: 2.027 ± 0.486
0.0SerXaa: 0.0 ± 0.0
Thr
6.081ThrAla: 6.081 ± 0.935
0.427ThrCys: 0.427 ± 0.221
4.374ThrAsp: 4.374 ± 0.825
4.908ThrGlu: 4.908 ± 0.591
2.667ThrPhe: 2.667 ± 0.671
4.481ThrGly: 4.481 ± 0.641
0.107ThrHis: 0.107 ± 0.107
4.481ThrIle: 4.481 ± 0.689
5.014ThrLys: 5.014 ± 0.632
4.908ThrLeu: 4.908 ± 0.766
1.067ThrMet: 1.067 ± 0.319
5.868ThrAsn: 5.868 ± 0.81
1.707ThrPro: 1.707 ± 0.369
2.774ThrGln: 2.774 ± 0.521
1.814ThrArg: 1.814 ± 0.382
3.841ThrSer: 3.841 ± 0.543
4.374ThrThr: 4.374 ± 0.801
5.548ThrVal: 5.548 ± 0.802
0.854ThrTrp: 0.854 ± 0.281
2.347ThrTyr: 2.347 ± 0.521
0.0ThrXaa: 0.0 ± 0.0
Val
3.734ValAla: 3.734 ± 0.514
0.32ValCys: 0.32 ± 0.174
3.841ValAsp: 3.841 ± 0.644
5.334ValGlu: 5.334 ± 0.663
3.094ValPhe: 3.094 ± 0.626
3.307ValGly: 3.307 ± 0.536
0.747ValHis: 0.747 ± 0.266
4.588ValIle: 4.588 ± 0.847
5.975ValLys: 5.975 ± 0.701
3.307ValLeu: 3.307 ± 0.612
1.814ValMet: 1.814 ± 0.392
3.307ValAsn: 3.307 ± 0.532
1.707ValPro: 1.707 ± 0.485
1.6ValGln: 1.6 ± 0.344
2.774ValArg: 2.774 ± 0.677
5.975ValSer: 5.975 ± 1.155
5.334ValThr: 5.334 ± 0.917
4.054ValVal: 4.054 ± 1.004
0.533ValTrp: 0.533 ± 0.238
2.667ValTyr: 2.667 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.283
0.32TrpCys: 0.32 ± 0.181
0.747TrpAsp: 0.747 ± 0.425
0.854TrpGlu: 0.854 ± 0.252
0.96TrpPhe: 0.96 ± 0.436
0.747TrpGly: 0.747 ± 0.285
0.213TrpHis: 0.213 ± 0.159
1.067TrpIle: 1.067 ± 0.286
0.854TrpLys: 0.854 ± 0.269
1.28TrpLeu: 1.28 ± 0.43
0.213TrpMet: 0.213 ± 0.164
1.494TrpAsn: 1.494 ± 0.461
0.0TrpPro: 0.0 ± 0.0
1.067TrpGln: 1.067 ± 0.297
0.32TrpArg: 0.32 ± 0.261
1.28TrpSer: 1.28 ± 0.42
0.533TrpThr: 0.533 ± 0.267
0.533TrpVal: 0.533 ± 0.249
0.213TrpTrp: 0.213 ± 0.136
0.747TrpTyr: 0.747 ± 0.262
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.92TyrAla: 1.92 ± 0.535
0.96TyrCys: 0.96 ± 0.419
2.454TyrAsp: 2.454 ± 0.663
3.627TyrGlu: 3.627 ± 0.8
2.134TyrPhe: 2.134 ± 0.412
2.881TyrGly: 2.881 ± 0.46
0.96TyrHis: 0.96 ± 0.279
3.201TyrIle: 3.201 ± 0.862
2.561TyrLys: 2.561 ± 0.561
3.521TyrLeu: 3.521 ± 0.735
1.067TyrMet: 1.067 ± 0.338
3.627TyrAsn: 3.627 ± 0.508
0.96TyrPro: 0.96 ± 0.318
2.027TyrGln: 2.027 ± 0.536
1.387TyrArg: 1.387 ± 0.434
1.92TyrSer: 1.92 ± 0.489
3.414TyrThr: 3.414 ± 0.989
2.134TyrVal: 2.134 ± 0.493
0.213TyrTrp: 0.213 ± 0.146
2.134TyrTyr: 2.134 ± 0.494
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (9374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski