Mycobacterium phage Lephleur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Rosebushvirus; unclassified Rosebushvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LGG7|A0A6B9LGG7_9CAUD Uncharacterized protein OS=Mycobacterium phage Lephleur OX=2686234 GN=87 PE=4 SV=1
MM1 pKa = 7.44SLYY4 pKa = 10.29TVITTEE10 pKa = 4.07ADD12 pKa = 3.33ATSHH16 pKa = 5.62EE17 pKa = 4.93VYY19 pKa = 9.34STHH22 pKa = 7.07VEE24 pKa = 3.73LAEE27 pKa = 4.3ACDD30 pKa = 3.98AVAEE34 pKa = 4.32MVDD37 pKa = 3.49EE38 pKa = 4.73LEE40 pKa = 4.86AEE42 pKa = 4.56EE43 pKa = 4.76VRR45 pKa = 11.84IALPADD51 pKa = 3.31IAAVDD56 pKa = 3.41IDD58 pKa = 4.16GEE60 pKa = 4.84SVSGTWYY67 pKa = 8.09ATHH70 pKa = 7.05RR71 pKa = 11.84GEE73 pKa = 4.53FLVAIFKK80 pKa = 10.62SS81 pKa = 3.73

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9L5W6|A0A6B9L5W6_9CAUD MuF-like minor capsid protein OS=Mycobacterium phage Lephleur OX=2686234 GN=13 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84RR4 pKa = 11.84CLRR7 pKa = 11.84VTCPVHH13 pKa = 6.72GIGRR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84AHH22 pKa = 5.97RR23 pKa = 11.84RR24 pKa = 11.84AAANARR30 pKa = 11.84EE31 pKa = 4.22ANFVAFFAHH40 pKa = 5.31QAEE43 pKa = 4.47LARR46 pKa = 11.84IEE48 pKa = 4.23RR49 pKa = 11.84KK50 pKa = 9.53KK51 pKa = 10.86RR52 pKa = 11.84LLAQAYY58 pKa = 8.63SGPGRR63 pKa = 11.84SGPEE67 pKa = 3.28LRR69 pKa = 11.84AWLDD73 pKa = 3.48ANQDD77 pKa = 3.23AAVRR81 pKa = 11.84LMTEE85 pKa = 4.03AMAADD90 pKa = 3.63LRR92 pKa = 11.84YY93 pKa = 9.69YY94 pKa = 10.46YY95 pKa = 10.86GYY97 pKa = 9.29ATALHH102 pKa = 6.34TEE104 pKa = 4.21LPGGIAA110 pKa = 4.01

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

21425

34

1881

232.9

25.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.19 ± 0.349

1.088 ± 0.137

6.273 ± 0.207

5.774 ± 0.246

2.572 ± 0.108

8.929 ± 0.543

2.091 ± 0.167

3.617 ± 0.174

2.74 ± 0.189

8.509 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.119

2.866 ± 0.133

6.1 ± 0.213

3.449 ± 0.161

6.6 ± 0.309

5.064 ± 0.18

6.856 ± 0.253

7.925 ± 0.225

1.993 ± 0.161

2.212 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski