Amino acid dipepetide frequency for Mycobacterium phage Lephleur

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.297AlaAla: 18.297 ± 1.511
1.494AlaCys: 1.494 ± 0.355
7.702AlaAsp: 7.702 ± 0.847
7.982AlaGlu: 7.982 ± 0.695
3.547AlaPhe: 3.547 ± 0.472
10.035AlaGly: 10.035 ± 1.046
2.521AlaHis: 2.521 ± 0.445
5.228AlaIle: 5.228 ± 0.662
4.108AlaLys: 4.108 ± 0.566
11.669AlaLeu: 11.669 ± 0.803
2.754AlaMet: 2.754 ± 0.367
4.248AlaAsn: 4.248 ± 0.484
7.655AlaPro: 7.655 ± 0.59
4.481AlaGln: 4.481 ± 0.465
8.962AlaArg: 8.962 ± 0.686
6.768AlaSer: 6.768 ± 0.649
8.729AlaThr: 8.729 ± 0.715
10.502AlaVal: 10.502 ± 0.636
2.194AlaTrp: 2.194 ± 0.333
2.987AlaTyr: 2.987 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
1.494CysAla: 1.494 ± 0.337
0.093CysCys: 0.093 ± 0.07
0.934CysAsp: 0.934 ± 0.229
0.7CysGlu: 0.7 ± 0.19
0.14CysPhe: 0.14 ± 0.116
1.494CysGly: 1.494 ± 0.315
0.187CysHis: 0.187 ± 0.121
0.233CysIle: 0.233 ± 0.093
0.14CysLys: 0.14 ± 0.08
0.607CysLeu: 0.607 ± 0.162
0.233CysMet: 0.233 ± 0.109
0.28CysAsn: 0.28 ± 0.112
0.7CysPro: 0.7 ± 0.169
0.28CysGln: 0.28 ± 0.116
0.84CysArg: 0.84 ± 0.274
0.327CysSer: 0.327 ± 0.128
0.7CysThr: 0.7 ± 0.2
0.84CysVal: 0.84 ± 0.197
0.42CysTrp: 0.42 ± 0.192
0.233CysTyr: 0.233 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
7.562AspAla: 7.562 ± 0.619
0.747AspCys: 0.747 ± 0.156
4.574AspAsp: 4.574 ± 0.647
3.454AspGlu: 3.454 ± 0.459
1.494AspPhe: 1.494 ± 0.356
5.788AspGly: 5.788 ± 0.502
1.4AspHis: 1.4 ± 0.278
1.867AspIle: 1.867 ± 0.221
2.007AspLys: 2.007 ± 0.241
6.208AspLeu: 6.208 ± 0.591
1.82AspMet: 1.82 ± 0.319
1.68AspAsn: 1.68 ± 0.305
4.434AspPro: 4.434 ± 0.457
1.727AspGln: 1.727 ± 0.272
3.687AspArg: 3.687 ± 0.42
3.081AspSer: 3.081 ± 0.431
4.434AspThr: 4.434 ± 0.443
4.108AspVal: 4.108 ± 0.406
1.354AspTrp: 1.354 ± 0.271
1.307AspTyr: 1.307 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
8.262GluAla: 8.262 ± 0.892
0.607GluCys: 0.607 ± 0.182
3.361GluAsp: 3.361 ± 0.559
2.847GluGlu: 2.847 ± 0.424
2.1GluPhe: 2.1 ± 0.264
3.734GluGly: 3.734 ± 0.39
1.634GluHis: 1.634 ± 0.259
2.24GluIle: 2.24 ± 0.333
1.074GluLys: 1.074 ± 0.238
6.255GluLeu: 6.255 ± 0.535
0.98GluMet: 0.98 ± 0.236
1.26GluAsn: 1.26 ± 0.276
3.454GluPro: 3.454 ± 0.5
2.707GluGln: 2.707 ± 0.42
4.388GluArg: 4.388 ± 0.492
2.054GluSer: 2.054 ± 0.281
2.707GluThr: 2.707 ± 0.395
5.508GluVal: 5.508 ± 0.507
1.354GluTrp: 1.354 ± 0.209
1.214GluTyr: 1.214 ± 0.241
0.0GluXaa: 0.0 ± 0.0
Phe
3.968PheAla: 3.968 ± 0.378
0.093PheCys: 0.093 ± 0.059
1.914PheAsp: 1.914 ± 0.307
1.587PheGlu: 1.587 ± 0.285
0.513PhePhe: 0.513 ± 0.169
2.427PheGly: 2.427 ± 0.417
0.42PheHis: 0.42 ± 0.151
0.887PheIle: 0.887 ± 0.263
0.7PheLys: 0.7 ± 0.202
1.96PheLeu: 1.96 ± 0.419
0.373PheMet: 0.373 ± 0.152
1.167PheAsn: 1.167 ± 0.197
1.214PhePro: 1.214 ± 0.233
1.12PheGln: 1.12 ± 0.187
1.867PheArg: 1.867 ± 0.321
1.027PheSer: 1.027 ± 0.233
1.727PheThr: 1.727 ± 0.276
1.82PheVal: 1.82 ± 0.329
0.467PheTrp: 0.467 ± 0.148
0.467PheTyr: 0.467 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
8.355GlyAla: 8.355 ± 1.078
0.747GlyCys: 0.747 ± 0.224
5.274GlyAsp: 5.274 ± 0.448
5.228GlyGlu: 5.228 ± 0.556
2.287GlyPhe: 2.287 ± 0.432
10.502GlyGly: 10.502 ± 2.142
2.007GlyHis: 2.007 ± 0.282
3.734GlyIle: 3.734 ± 0.425
2.754GlyLys: 2.754 ± 0.483
7.655GlyLeu: 7.655 ± 0.668
1.727GlyMet: 1.727 ± 0.237
2.894GlyAsn: 2.894 ± 0.32
4.481GlyPro: 4.481 ± 0.676
3.874GlyGln: 3.874 ± 0.569
4.341GlyArg: 4.341 ± 0.559
4.201GlySer: 4.201 ± 0.506
7.235GlyThr: 7.235 ± 0.613
7.235GlyVal: 7.235 ± 0.678
2.147GlyTrp: 2.147 ± 0.341
2.614GlyTyr: 2.614 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
2.054HisAla: 2.054 ± 0.375
0.28HisCys: 0.28 ± 0.126
1.26HisAsp: 1.26 ± 0.223
1.354HisGlu: 1.354 ± 0.251
0.607HisPhe: 0.607 ± 0.156
1.82HisGly: 1.82 ± 0.317
0.653HisHis: 0.653 ± 0.181
0.607HisIle: 0.607 ± 0.171
0.373HisLys: 0.373 ± 0.156
2.24HisLeu: 2.24 ± 0.393
0.373HisMet: 0.373 ± 0.129
0.7HisAsn: 0.7 ± 0.185
1.12HisPro: 1.12 ± 0.24
0.56HisGln: 0.56 ± 0.172
1.447HisArg: 1.447 ± 0.312
1.214HisSer: 1.214 ± 0.23
1.634HisThr: 1.634 ± 0.327
1.214HisVal: 1.214 ± 0.28
0.887HisTrp: 0.887 ± 0.198
0.513HisTyr: 0.513 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
5.368IleAla: 5.368 ± 0.45
0.233IleCys: 0.233 ± 0.093
3.081IleAsp: 3.081 ± 0.371
2.427IleGlu: 2.427 ± 0.373
0.42IlePhe: 0.42 ± 0.15
3.407IleGly: 3.407 ± 0.362
0.56IleHis: 0.56 ± 0.156
1.167IleIle: 1.167 ± 0.288
1.354IleLys: 1.354 ± 0.26
2.567IleLeu: 2.567 ± 0.305
0.56IleMet: 0.56 ± 0.174
1.307IleAsn: 1.307 ± 0.256
1.727IlePro: 1.727 ± 0.28
1.26IleGln: 1.26 ± 0.243
1.914IleArg: 1.914 ± 0.296
2.24IleSer: 2.24 ± 0.368
3.127IleThr: 3.127 ± 0.444
2.754IleVal: 2.754 ± 0.399
0.28IleTrp: 0.28 ± 0.121
0.42IleTyr: 0.42 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
4.248LysAla: 4.248 ± 0.644
0.42LysCys: 0.42 ± 0.145
1.26LysAsp: 1.26 ± 0.284
1.074LysGlu: 1.074 ± 0.236
0.653LysPhe: 0.653 ± 0.17
2.287LysGly: 2.287 ± 0.326
0.98LysHis: 0.98 ± 0.212
0.794LysIle: 0.794 ± 0.228
1.027LysLys: 1.027 ± 0.26
2.567LysLeu: 2.567 ± 0.356
0.56LysMet: 0.56 ± 0.147
0.84LysAsn: 0.84 ± 0.244
0.887LysPro: 0.887 ± 0.205
0.653LysGln: 0.653 ± 0.203
2.147LysArg: 2.147 ± 0.307
1.214LysSer: 1.214 ± 0.293
1.82LysThr: 1.82 ± 0.289
2.801LysVal: 2.801 ± 0.362
0.513LysTrp: 0.513 ± 0.163
0.653LysTyr: 0.653 ± 0.157
0.0LysXaa: 0.0 ± 0.0
Leu
14.19LeuAla: 14.19 ± 0.903
0.794LeuCys: 0.794 ± 0.185
5.274LeuAsp: 5.274 ± 0.554
4.714LeuGlu: 4.714 ± 0.49
1.774LeuPhe: 1.774 ± 0.279
7.001LeuGly: 7.001 ± 0.639
1.307LeuHis: 1.307 ± 0.276
3.641LeuIle: 3.641 ± 0.438
2.007LeuLys: 2.007 ± 0.399
7.235LeuLeu: 7.235 ± 0.571
2.054LeuMet: 2.054 ± 0.287
2.147LeuAsn: 2.147 ± 0.334
5.741LeuPro: 5.741 ± 0.56
2.194LeuGln: 2.194 ± 0.345
5.881LeuArg: 5.881 ± 0.504
4.434LeuSer: 4.434 ± 0.478
5.181LeuThr: 5.181 ± 0.482
6.441LeuVal: 6.441 ± 0.501
1.447LeuTrp: 1.447 ± 0.264
1.634LeuTyr: 1.634 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
3.547MetAla: 3.547 ± 0.399
0.14MetCys: 0.14 ± 0.072
1.074MetAsp: 1.074 ± 0.217
0.747MetGlu: 0.747 ± 0.214
0.467MetPhe: 0.467 ± 0.143
0.794MetGly: 0.794 ± 0.202
0.56MetHis: 0.56 ± 0.149
0.653MetIle: 0.653 ± 0.182
0.42MetLys: 0.42 ± 0.113
1.774MetLeu: 1.774 ± 0.27
0.28MetMet: 0.28 ± 0.099
0.653MetAsn: 0.653 ± 0.177
1.727MetPro: 1.727 ± 0.294
0.607MetGln: 0.607 ± 0.196
1.354MetArg: 1.354 ± 0.263
2.24MetSer: 2.24 ± 0.307
2.007MetThr: 2.007 ± 0.356
1.68MetVal: 1.68 ± 0.255
0.28MetTrp: 0.28 ± 0.138
0.513MetTyr: 0.513 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.968AsnAla: 3.968 ± 0.318
0.327AsnCys: 0.327 ± 0.14
1.82AsnAsp: 1.82 ± 0.336
1.214AsnGlu: 1.214 ± 0.251
0.56AsnPhe: 0.56 ± 0.171
3.687AsnGly: 3.687 ± 0.327
0.56AsnHis: 0.56 ± 0.18
1.26AsnIle: 1.26 ± 0.264
0.607AsnLys: 0.607 ± 0.204
2.521AsnLeu: 2.521 ± 0.341
0.7AsnMet: 0.7 ± 0.16
0.7AsnAsn: 0.7 ± 0.191
1.774AsnPro: 1.774 ± 0.316
1.167AsnGln: 1.167 ± 0.27
1.447AsnArg: 1.447 ± 0.256
1.587AsnSer: 1.587 ± 0.281
1.914AsnThr: 1.914 ± 0.332
1.774AsnVal: 1.774 ± 0.284
0.653AsnTrp: 0.653 ± 0.203
0.42AsnTyr: 0.42 ± 0.127
0.0AsnXaa: 0.0 ± 0.0
Pro
6.768ProAla: 6.768 ± 0.713
0.42ProCys: 0.42 ± 0.146
4.341ProAsp: 4.341 ± 0.421
5.228ProGlu: 5.228 ± 0.527
1.214ProPhe: 1.214 ± 0.244
6.628ProGly: 6.628 ± 0.709
1.214ProHis: 1.214 ± 0.289
1.587ProIle: 1.587 ± 0.354
1.774ProLys: 1.774 ± 0.328
3.734ProLeu: 3.734 ± 0.439
0.794ProMet: 0.794 ± 0.194
1.774ProAsn: 1.774 ± 0.307
4.714ProPro: 4.714 ± 0.601
1.587ProGln: 1.587 ± 0.315
2.614ProArg: 2.614 ± 0.349
3.267ProSer: 3.267 ± 0.401
5.041ProThr: 5.041 ± 0.572
6.348ProVal: 6.348 ± 0.502
0.887ProTrp: 0.887 ± 0.223
1.074ProTyr: 1.074 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
5.601GlnAla: 5.601 ± 0.596
0.187GlnCys: 0.187 ± 0.103
1.68GlnAsp: 1.68 ± 0.251
1.774GlnGlu: 1.774 ± 0.251
1.354GlnPhe: 1.354 ± 0.263
2.24GlnGly: 2.24 ± 0.36
0.56GlnHis: 0.56 ± 0.144
1.26GlnIle: 1.26 ± 0.252
0.653GlnLys: 0.653 ± 0.188
3.734GlnLeu: 3.734 ± 0.446
0.98GlnMet: 0.98 ± 0.235
0.513GlnAsn: 0.513 ± 0.163
1.587GlnPro: 1.587 ± 0.238
1.867GlnGln: 1.867 ± 0.308
2.754GlnArg: 2.754 ± 0.334
1.447GlnSer: 1.447 ± 0.266
1.587GlnThr: 1.587 ± 0.298
3.081GlnVal: 3.081 ± 0.436
1.027GlnTrp: 1.027 ± 0.243
0.607GlnTyr: 0.607 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
8.075ArgAla: 8.075 ± 0.752
1.074ArgCys: 1.074 ± 0.244
3.921ArgAsp: 3.921 ± 0.486
4.061ArgGlu: 4.061 ± 0.565
1.82ArgPhe: 1.82 ± 0.329
5.274ArgGly: 5.274 ± 0.533
1.727ArgHis: 1.727 ± 0.289
2.521ArgIle: 2.521 ± 0.327
1.54ArgLys: 1.54 ± 0.294
5.835ArgLeu: 5.835 ± 0.527
1.96ArgMet: 1.96 ± 0.371
2.054ArgAsn: 2.054 ± 0.315
3.127ArgPro: 3.127 ± 0.404
2.334ArgGln: 2.334 ± 0.338
5.648ArgArg: 5.648 ± 0.586
2.801ArgSer: 2.801 ± 0.332
4.154ArgThr: 4.154 ± 0.503
4.574ArgVal: 4.574 ± 0.511
1.54ArgTrp: 1.54 ± 0.279
1.96ArgTyr: 1.96 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
6.208SerAla: 6.208 ± 0.595
0.607SerCys: 0.607 ± 0.183
3.594SerAsp: 3.594 ± 0.412
2.614SerGlu: 2.614 ± 0.372
1.26SerPhe: 1.26 ± 0.291
6.255SerGly: 6.255 ± 0.584
0.56SerHis: 0.56 ± 0.163
1.867SerIle: 1.867 ± 0.34
1.727SerLys: 1.727 ± 0.32
3.781SerLeu: 3.781 ± 0.485
1.587SerMet: 1.587 ± 0.295
1.447SerAsn: 1.447 ± 0.259
3.034SerPro: 3.034 ± 0.396
1.354SerGln: 1.354 ± 0.209
2.847SerArg: 2.847 ± 0.398
2.381SerSer: 2.381 ± 0.352
3.594SerThr: 3.594 ± 0.444
3.968SerVal: 3.968 ± 0.489
0.98SerTrp: 0.98 ± 0.232
0.98SerTyr: 0.98 ± 0.269
0.0SerXaa: 0.0 ± 0.0
Thr
8.122ThrAla: 8.122 ± 0.56
0.747ThrCys: 0.747 ± 0.209
3.827ThrAsp: 3.827 ± 0.465
3.547ThrGlu: 3.547 ± 0.375
2.194ThrPhe: 2.194 ± 0.342
6.488ThrGly: 6.488 ± 0.662
1.447ThrHis: 1.447 ± 0.259
2.941ThrIle: 2.941 ± 0.481
2.521ThrLys: 2.521 ± 0.383
4.994ThrLeu: 4.994 ± 0.564
1.26ThrMet: 1.26 ± 0.271
1.82ThrAsn: 1.82 ± 0.299
5.788ThrPro: 5.788 ± 0.545
2.194ThrGln: 2.194 ± 0.359
3.547ThrArg: 3.547 ± 0.378
4.014ThrSer: 4.014 ± 0.502
4.808ThrThr: 4.808 ± 0.782
5.181ThrVal: 5.181 ± 0.549
1.54ThrTrp: 1.54 ± 0.288
1.587ThrTyr: 1.587 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
11.296ValAla: 11.296 ± 0.867
0.98ValCys: 0.98 ± 0.225
5.134ValAsp: 5.134 ± 0.436
4.621ValGlu: 4.621 ± 0.513
1.867ValPhe: 1.867 ± 0.26
6.021ValGly: 6.021 ± 0.662
1.54ValHis: 1.54 ± 0.308
2.474ValIle: 2.474 ± 0.282
1.54ValLys: 1.54 ± 0.259
6.255ValLeu: 6.255 ± 0.482
1.587ValMet: 1.587 ± 0.335
1.914ValAsn: 1.914 ± 0.324
5.601ValPro: 5.601 ± 0.62
2.987ValGln: 2.987 ± 0.426
6.301ValArg: 6.301 ± 0.718
4.434ValSer: 4.434 ± 0.515
5.228ValThr: 5.228 ± 0.501
6.441ValVal: 6.441 ± 0.784
1.214ValTrp: 1.214 ± 0.292
1.82ValTyr: 1.82 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
2.194TrpAla: 2.194 ± 0.381
0.513TrpCys: 0.513 ± 0.177
1.12TrpAsp: 1.12 ± 0.186
1.307TrpGlu: 1.307 ± 0.24
0.84TrpPhe: 0.84 ± 0.226
1.027TrpGly: 1.027 ± 0.271
0.747TrpHis: 0.747 ± 0.185
0.513TrpIle: 0.513 ± 0.144
0.373TrpLys: 0.373 ± 0.118
1.727TrpLeu: 1.727 ± 0.314
0.42TrpMet: 0.42 ± 0.164
0.653TrpAsn: 0.653 ± 0.19
1.26TrpPro: 1.26 ± 0.232
0.794TrpGln: 0.794 ± 0.198
1.447TrpArg: 1.447 ± 0.272
1.4TrpSer: 1.4 ± 0.292
1.12TrpThr: 1.12 ± 0.239
1.354TrpVal: 1.354 ± 0.29
0.513TrpTrp: 0.513 ± 0.165
0.607TrpTyr: 0.607 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.334TyrAla: 2.334 ± 0.304
0.373TyrCys: 0.373 ± 0.126
1.587TyrAsp: 1.587 ± 0.289
1.26TyrGlu: 1.26 ± 0.254
0.607TyrPhe: 0.607 ± 0.201
2.381TyrGly: 2.381 ± 0.282
0.327TyrHis: 0.327 ± 0.126
0.7TyrIle: 0.7 ± 0.188
0.7TyrLys: 0.7 ± 0.19
1.774TyrLeu: 1.774 ± 0.357
0.467TyrMet: 0.467 ± 0.143
0.607TyrAsn: 0.607 ± 0.171
0.98TyrPro: 0.98 ± 0.188
0.747TyrGln: 0.747 ± 0.201
2.567TyrArg: 2.567 ± 0.39
0.513TyrSer: 0.513 ± 0.138
1.82TyrThr: 1.82 ± 0.285
1.634TyrVal: 1.634 ± 0.262
0.233TyrTrp: 0.233 ± 0.1
0.513TyrTyr: 0.513 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (21425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski