Bartonella tamiae Th239
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1978 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J0R7F7|J0R7F7_9RHIZ Uncharacterized protein OS=Bartonella tamiae Th239 OX=1094558 GN=ME5_00048 PE=4 SV=1
MM1 pKa = 7.7 AYY3 pKa = 9.18 IYY5 pKa = 10.74 YY6 pKa = 10.17 SIPLFNDD13 pKa = 2.73 NGRR16 pKa = 11.84 QISEE20 pKa = 4.24 LDD22 pKa = 3.48 GKK24 pKa = 10.73 VEE26 pKa = 4.39 FSVGLDD32 pKa = 3.05 NGEE35 pKa = 4.01 PFAEE39 pKa = 4.36 IDD41 pKa = 4.64 CIYY44 pKa = 11.16 ADD46 pKa = 4.0 DD47 pKa = 4.38 FEE49 pKa = 5.59 GFEE52 pKa = 4.12 ICYY55 pKa = 9.93 SANSDD60 pKa = 3.74 TPFDD64 pKa = 3.51 LTVFLRR70 pKa = 11.84 AKK72 pKa = 10.08 EE73 pKa = 3.89 YY74 pKa = 11.19 LEE76 pKa = 4.85 QSDD79 pKa = 4.4 AFQNEE84 pKa = 4.67 AINEE88 pKa = 4.4 DD89 pKa = 3.4 GSFVYY94 pKa = 10.07 EE95 pKa = 4.07 GRR97 pKa = 11.84 GSNDD101 pKa = 2.91 PDD103 pKa = 3.9 GYY105 pKa = 10.12 FKK107 pKa = 10.77 RR108 pKa = 11.84 VAA110 pKa = 3.45
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|J1K0T5|J1K0T5_9RHIZ 30S ribosomal protein S18 OS=Bartonella tamiae Th239 OX=1094558 GN=rpsR PE=3 SV=1
MM1 pKa = 7.2 QVNLKK6 pKa = 10.31 RR7 pKa = 11.84 KK8 pKa = 7.92 AQEE11 pKa = 3.69 RR12 pKa = 11.84 QSFVTIIVVRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 MGQRR28 pKa = 11.84 SQGILAIGQNRR39 pKa = 11.84 FICALGASGIVSQKK53 pKa = 10.57 YY54 pKa = 9.45 EE55 pKa = 4.23 GDD57 pKa = 3.45 GATPRR62 pKa = 11.84 GVMKK66 pKa = 10.39 VLQGFRR72 pKa = 11.84 QKK74 pKa = 10.58 NVHH77 pKa = 6.21 SRR79 pKa = 11.84 TKK81 pKa = 9.49 HH82 pKa = 4.91 CLKK85 pKa = 9.96 MRR87 pKa = 11.84 IIGNRR92 pKa = 11.84 DD93 pKa = 2.76 GWCDD97 pKa = 3.73 EE98 pKa = 4.12 NWDD101 pKa = 3.73 ANYY104 pKa = 10.18 NRR106 pKa = 11.84 PVRR109 pKa = 11.84 LPYY112 pKa = 9.9 RR113 pKa = 11.84 ASAEE117 pKa = 3.74 EE118 pKa = 3.99 MQRR121 pKa = 11.84 KK122 pKa = 9.82 DD123 pKa = 3.66 EE124 pKa = 4.48 IYY126 pKa = 11.3 DD127 pKa = 3.22 MGLIVDD133 pKa = 3.88 WNIQKK138 pKa = 10.52 RR139 pKa = 11.84 VMNRR143 pKa = 11.84 GSAIFMHH150 pKa = 7.19 LARR153 pKa = 11.84 KK154 pKa = 9.96 GYY156 pKa = 8.3 TPTRR160 pKa = 11.84 GCIALSRR167 pKa = 11.84 RR168 pKa = 11.84 DD169 pKa = 3.51 MEE171 pKa = 4.36 RR172 pKa = 11.84 VFTVVSNKK180 pKa = 8.28 TRR182 pKa = 11.84 LLILGG187 pKa = 4.04
Molecular weight: 21.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.78
IPC_protein 10.774
Toseland 10.935
ProMoST 10.804
Dawson 11.008
Bjellqvist 10.774
Wikipedia 11.272
Rodwell 11.125
Grimsley 11.038
Solomon 11.213
Lehninger 11.169
Nozaki 10.935
DTASelect 10.774
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 10.847
IPC_peptide 11.213
IPC2_peptide 9.984
IPC2.peptide.svr19 8.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1978
0
1978
619200
30
4052
313.0
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.719 ± 0.056
0.883 ± 0.018
5.794 ± 0.049
5.681 ± 0.056
4.422 ± 0.043
7.053 ± 0.071
2.423 ± 0.028
7.535 ± 0.047
5.741 ± 0.056
9.84 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.029
4.425 ± 0.058
4.004 ± 0.033
3.813 ± 0.037
5.234 ± 0.055
6.216 ± 0.047
5.351 ± 0.05
6.331 ± 0.046
1.121 ± 0.022
2.81 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here