Oleiphilus sp. HI0066

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oleiphilaceae; Oleiphilus; unclassified Oleiphilus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3585 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A657AUS8|A0A657AUS8_9GAMM Deoxyribodipyrimidine photolyase OS=Oleiphilus sp. HI0066 OX=1822242 GN=A3738_09590 PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.36KK3 pKa = 10.24VVLASALLAASVSAQAGLITTVEE26 pKa = 4.18AEE28 pKa = 4.11NQFVSTVSDD37 pKa = 3.44SLDD40 pKa = 3.44IIDD43 pKa = 4.52FDD45 pKa = 4.21EE46 pKa = 5.32GNVGNISGDD55 pKa = 3.41YY56 pKa = 9.02TVYY59 pKa = 9.75STPSGTNQSAPPFGITSEE77 pKa = 4.22YY78 pKa = 10.87LSVPNPNQNGSATILLDD95 pKa = 3.07EE96 pKa = 4.76TYY98 pKa = 11.38NFFGLFWGSVDD109 pKa = 4.58DD110 pKa = 4.19YY111 pKa = 12.07NSITFYY117 pKa = 11.15NGDD120 pKa = 3.37VSDD123 pKa = 5.14DD124 pKa = 3.82NNIVASISGDD134 pKa = 3.66QIDD137 pKa = 5.75DD138 pKa = 3.98LLADD142 pKa = 4.72GNQVSWTSNRR152 pKa = 11.84FVNFFFTDD160 pKa = 2.95GDD162 pKa = 3.78VFDD165 pKa = 5.81RR166 pKa = 11.84YY167 pKa = 11.24VLTSNGYY174 pKa = 9.8AFEE177 pKa = 4.94SDD179 pKa = 2.67NHH181 pKa = 6.87SYY183 pKa = 11.7GNVSVSVSEE192 pKa = 4.21PATLALFGLGLAALGFARR210 pKa = 11.84RR211 pKa = 11.84KK212 pKa = 9.56AA213 pKa = 3.35

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A657ATS8|A0A657ATS8_9GAMM Protein TolR (Fragment) OS=Oleiphilus sp. HI0066 OX=1822242 GN=A3738_19525 PE=3 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84IAGVNIPDD11 pKa = 3.76NKK13 pKa = 10.22HH14 pKa = 5.79AVISLTYY21 pKa = 9.11IYY23 pKa = 10.96GVGRR27 pKa = 11.84TTAYY31 pKa = 9.65KK32 pKa = 10.57LCDD35 pKa = 3.55ATGVTPTAKK44 pKa = 10.03VSSLTEE50 pKa = 3.81EE51 pKa = 4.13QLDD54 pKa = 3.83SLRR57 pKa = 11.84TEE59 pKa = 4.14VTKK62 pKa = 9.96LTVEE66 pKa = 3.92GDD68 pKa = 3.39LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.69VQMNIKK78 pKa = 9.87RR79 pKa = 11.84LKK81 pKa = 10.79DD82 pKa = 3.39LGCFRR87 pKa = 11.84GLRR90 pKa = 11.84HH91 pKa = 5.81RR92 pKa = 11.84HH93 pKa = 4.7SLPVRR98 pKa = 11.84GQRR101 pKa = 11.84SKK103 pKa = 10.14TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.72GPRR113 pKa = 11.84KK114 pKa = 9.36PIRR117 pKa = 11.84KK118 pKa = 9.01

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3585

0

3585

1085609

35

3444

302.8

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.839 ± 0.04

1.009 ± 0.011

5.89 ± 0.04

6.74 ± 0.043

4.185 ± 0.03

6.864 ± 0.039

2.14 ± 0.02

6.271 ± 0.035

5.195 ± 0.043

10.348 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.019

4.129 ± 0.029

3.813 ± 0.02

4.157 ± 0.028

4.689 ± 0.033

7.174 ± 0.034

5.047 ± 0.033

6.996 ± 0.041

1.104 ± 0.016

2.959 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski