Mesotoga sp. SC_3PWM13N19

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Kosmotogales; Kosmotogaceae; Mesotoga; unclassified Mesotoga

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328IK35|A0A328IK35_9BACT Uncharacterized protein OS=Mesotoga sp. SC_3PWM13N19 OX=1487951 GN=DS66_06215 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.25RR3 pKa = 11.84AIGFVAIGLLVLVIAGCFSLDD24 pKa = 3.36QKK26 pKa = 11.13AVNSEE31 pKa = 4.06EE32 pKa = 3.91VSFILNDD39 pKa = 4.16DD40 pKa = 3.65GSISIKK46 pKa = 10.39ALKK49 pKa = 9.63EE50 pKa = 3.55LTGIDD55 pKa = 3.86VIVAASIEE63 pKa = 4.22EE64 pKa = 3.99EE65 pKa = 4.87AITVDD70 pKa = 4.81DD71 pKa = 4.32SLLKK75 pKa = 10.34IVTHH79 pKa = 6.18SDD81 pKa = 2.78EE82 pKa = 4.27GTTRR86 pKa = 11.84IAIVSTSANIRR97 pKa = 11.84EE98 pKa = 4.36GEE100 pKa = 4.14EE101 pKa = 3.55IARR104 pKa = 11.84IDD106 pKa = 4.6GNFTTLKK113 pKa = 10.02NLVISSSAKK122 pKa = 8.5YY123 pKa = 10.9LEE125 pKa = 4.38MPSPKK130 pKa = 9.05TQAKK134 pKa = 9.73PPFPDD139 pKa = 5.06GISIVDD145 pKa = 3.64GSIDD149 pKa = 3.55KK150 pKa = 10.95ASNGYY155 pKa = 9.64FLVHH159 pKa = 6.7AEE161 pKa = 4.21NVNGFAGVEE170 pKa = 3.71ITITYY175 pKa = 9.13DD176 pKa = 3.11PQYY179 pKa = 11.02IIIDD183 pKa = 3.75KK184 pKa = 10.97SKK186 pKa = 10.7GEE188 pKa = 4.0QGVTPLNSFGTGLLIVQHH206 pKa = 5.9TEE208 pKa = 3.35NTITITSAFNSAKK221 pKa = 10.29SVNCEE226 pKa = 3.78YY227 pKa = 10.17IYY229 pKa = 10.23QIHH232 pKa = 5.98FVASLIEE239 pKa = 4.31GKK241 pKa = 7.71TLIGLSGEE249 pKa = 4.13VRR251 pKa = 11.84DD252 pKa = 4.63ANTGLIQTVFHH263 pKa = 7.28DD264 pKa = 4.33GEE266 pKa = 4.28ITIGGPKK273 pKa = 10.37LIGDD277 pKa = 4.55FDD279 pKa = 4.15NSNKK283 pKa = 9.39VDD285 pKa = 4.06LPDD288 pKa = 5.66FILFARR294 pKa = 11.84SYY296 pKa = 10.44GSVLGGEE303 pKa = 4.48GEE305 pKa = 4.4YY306 pKa = 10.34IEE308 pKa = 5.18TYY310 pKa = 10.43DD311 pKa = 4.6IAPAEE316 pKa = 4.32DD317 pKa = 4.22KK318 pKa = 11.27YY319 pKa = 10.88QGVWAGIYY327 pKa = 9.74DD328 pKa = 3.71SCATDD333 pKa = 3.98GEE335 pKa = 4.31IDD337 pKa = 3.89LVDD340 pKa = 4.93FIIFARR346 pKa = 11.84NYY348 pKa = 10.17GKK350 pKa = 10.04NKK352 pKa = 9.36PNEE355 pKa = 4.04PPFISVPDD363 pKa = 3.59QQVAQGDD370 pKa = 4.17TLEE373 pKa = 5.44LDD375 pKa = 3.8LLNYY379 pKa = 10.61SSDD382 pKa = 3.88PEE384 pKa = 5.14GDD386 pKa = 3.35SLLFILEE393 pKa = 3.96VDD395 pKa = 3.88SPGTITDD402 pKa = 4.17SIYY405 pKa = 10.7SYY407 pKa = 11.04TPDD410 pKa = 4.4FEE412 pKa = 4.79TLGNQTVDD420 pKa = 2.8IAVEE424 pKa = 4.07DD425 pKa = 3.84TAGNMMTDD433 pKa = 3.15TFIIEE438 pKa = 4.08VTKK441 pKa = 10.08TNRR444 pKa = 11.84PPVTDD449 pKa = 5.7GIPDD453 pKa = 3.28QTIAEE458 pKa = 4.48GEE460 pKa = 4.35TLNLEE465 pKa = 4.13LLDD468 pKa = 4.06YY469 pKa = 11.32FEE471 pKa = 6.61DD472 pKa = 4.68PDD474 pKa = 4.47GDD476 pKa = 3.81NLVFAVISGVGTVIGSEE493 pKa = 4.11YY494 pKa = 9.98TYY496 pKa = 11.23SPNYY500 pKa = 9.52DD501 pKa = 2.98AAGTYY506 pKa = 9.53QVTIKK511 pKa = 10.75AADD514 pKa = 3.7GNGGEE519 pKa = 4.35VEE521 pKa = 4.45TTFTLTVEE529 pKa = 4.27NTNRR533 pKa = 11.84PPEE536 pKa = 4.25EE537 pKa = 4.13PQLVSPEE544 pKa = 3.81NAALLINTTSVEE556 pKa = 4.1LSWNCSDD563 pKa = 5.7PDD565 pKa = 4.08GQLLTYY571 pKa = 10.33DD572 pKa = 3.77VYY574 pKa = 11.08FGEE577 pKa = 5.34DD578 pKa = 3.0IEE580 pKa = 4.28ALEE583 pKa = 4.69VIATQSATSLHH594 pKa = 5.22VQEE597 pKa = 4.3ITAGKK602 pKa = 7.8TYY604 pKa = 10.3FWMIVARR611 pKa = 11.84DD612 pKa = 3.45PHH614 pKa = 6.51GAEE617 pKa = 4.17TASEE621 pKa = 4.7VYY623 pKa = 10.6SFSTDD628 pKa = 2.97YY629 pKa = 11.32YY630 pKa = 10.03LIPGGEE636 pKa = 4.25FEE638 pKa = 5.48GMISGYY644 pKa = 11.66ALMTKK649 pKa = 10.34ANSPYY654 pKa = 9.99IITGDD659 pKa = 3.14IVVEE663 pKa = 4.48SGAQLIIEE671 pKa = 4.64PGVEE675 pKa = 3.88VRR677 pKa = 11.84FTYY680 pKa = 10.48ISDD683 pKa = 3.87PDD685 pKa = 3.75NNGQEE690 pKa = 4.29DD691 pKa = 4.53TNAADD696 pKa = 4.56LIVYY700 pKa = 8.52GKK702 pKa = 10.5LFAEE706 pKa = 5.33GSEE709 pKa = 4.29TEE711 pKa = 4.13PLLFTSNEE719 pKa = 3.92TPALKK724 pKa = 10.49GDD726 pKa = 3.06WGGIRR731 pKa = 11.84FASSEE736 pKa = 4.15GTSKK740 pKa = 10.32ISHH743 pKa = 6.17ATIEE747 pKa = 4.15MAKK750 pKa = 10.45DD751 pKa = 4.05GVWTSSNCNIEE762 pKa = 3.74ISNCEE767 pKa = 3.21IRR769 pKa = 11.84TNIDD773 pKa = 3.11YY774 pKa = 11.02GVLLGSNSNATIVDD788 pKa = 3.83STIHH792 pKa = 6.27LNATGISNYY801 pKa = 8.35GTMIIRR807 pKa = 11.84GCTISSSGGTGIYY820 pKa = 10.45SNGVYY825 pKa = 10.01IEE827 pKa = 4.26IYY829 pKa = 7.51EE830 pKa = 4.21TLIGEE835 pKa = 4.23NSSTGITLHH844 pKa = 6.24NSSSNSRR851 pKa = 11.84VQNCHH856 pKa = 5.92FLDD859 pKa = 3.88NGSYY863 pKa = 10.63GIEE866 pKa = 4.08GYY868 pKa = 10.13RR869 pKa = 11.84FEE871 pKa = 5.69VIDD874 pKa = 4.52SSFARR879 pKa = 11.84NGNHH883 pKa = 7.33AISGSNCVVEE893 pKa = 4.89GCLFTEE899 pKa = 4.56SIKK902 pKa = 10.8GIRR905 pKa = 11.84GSSITAVNNNFEE917 pKa = 4.77GEE919 pKa = 4.43FCSSFYY925 pKa = 10.99DD926 pKa = 4.12DD927 pKa = 3.93YY928 pKa = 11.79SGVYY932 pKa = 9.5IDD934 pKa = 4.04GQALISHH941 pKa = 6.19NVFSGFATGVWLATSSEE958 pKa = 4.08VTIQDD963 pKa = 3.42NLVTGNYY970 pKa = 9.85RR971 pKa = 11.84GICFASVDD979 pKa = 4.14GQNLTCSNNNIYY991 pKa = 10.53DD992 pKa = 3.61NRR994 pKa = 11.84DD995 pKa = 2.86WNVYY999 pKa = 10.13NDD1001 pKa = 3.53TNQRR1005 pKa = 11.84VAITNSFWGTDD1016 pKa = 3.3NEE1018 pKa = 4.46STIKK1022 pKa = 10.82SKK1024 pKa = 10.75IFDD1027 pKa = 3.89YY1028 pKa = 11.61YY1029 pKa = 10.63EE1030 pKa = 4.75DD1031 pKa = 3.78SSKK1034 pKa = 11.41GPVSYY1039 pKa = 10.23TGFKK1043 pKa = 10.61SSLIDD1048 pKa = 3.45GATSSKK1054 pKa = 10.3RR1055 pKa = 11.84IALIPYY1061 pKa = 9.64DD1062 pKa = 3.52RR1063 pKa = 5.93

Molecular weight: 114.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328IT45|A0A328IT45_9BACT Sugar ABC transporter ATP-binding protein (Fragment) OS=Mesotoga sp. SC_3PWM13N19 OX=1487951 GN=DS66_03210 PE=4 SV=1
MM1 pKa = 7.17VKK3 pKa = 10.42DD4 pKa = 3.27NRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84GRR10 pKa = 11.84SRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.64CRR16 pKa = 11.84LCGTKK21 pKa = 9.17TKK23 pKa = 10.96YY24 pKa = 9.91IDD26 pKa = 3.65YY27 pKa = 10.49KK28 pKa = 10.17NTSLLRR34 pKa = 11.84DD35 pKa = 3.58YY36 pKa = 10.1VTEE39 pKa = 4.11KK40 pKa = 11.08GKK42 pKa = 10.48IIPKK46 pKa = 10.17RR47 pKa = 11.84ITGNCAKK54 pKa = 9.36HH55 pKa = 4.62QRR57 pKa = 11.84MVKK60 pKa = 9.9EE61 pKa = 4.51AIQRR65 pKa = 11.84ARR67 pKa = 11.84FMALLPYY74 pKa = 9.86TKK76 pKa = 10.23EE77 pKa = 3.82

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1662

0

1662

494837

32

2917

297.7

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.624 ± 0.057

0.809 ± 0.018

5.456 ± 0.047

7.729 ± 0.067

4.938 ± 0.052

7.34 ± 0.056

1.506 ± 0.025

7.511 ± 0.048

5.793 ± 0.057

10.031 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.027

3.765 ± 0.032

3.7 ± 0.031

2.285 ± 0.026

5.581 ± 0.048

7.492 ± 0.054

4.903 ± 0.041

7.542 ± 0.049

1.02 ± 0.023

3.418 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski