Amino acid dipepetide frequency for Mesotoga sp. SC_3PWM13N19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.276AlaAla: 5.276 ± 0.137
0.562AlaCys: 0.562 ± 0.036
3.098AlaAsp: 3.098 ± 0.087
4.353AlaGlu: 4.353 ± 0.096
3.425AlaPhe: 3.425 ± 0.087
5.198AlaGly: 5.198 ± 0.107
0.994AlaHis: 0.994 ± 0.039
5.485AlaIle: 5.485 ± 0.107
3.642AlaLys: 3.642 ± 0.105
7.16AlaLeu: 7.16 ± 0.138
1.78AlaMet: 1.78 ± 0.067
2.294AlaAsn: 2.294 ± 0.076
1.744AlaPro: 1.744 ± 0.048
1.427AlaGln: 1.427 ± 0.055
3.69AlaArg: 3.69 ± 0.089
4.737AlaSer: 4.737 ± 0.092
3.029AlaThr: 3.029 ± 0.089
5.713AlaVal: 5.713 ± 0.128
0.568AlaTrp: 0.568 ± 0.038
2.069AlaTyr: 2.069 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.42CysAla: 0.42 ± 0.034
0.073CysCys: 0.073 ± 0.012
0.521CysAsp: 0.521 ± 0.03
0.56CysGlu: 0.56 ± 0.032
0.388CysPhe: 0.388 ± 0.03
0.863CysGly: 0.863 ± 0.057
0.188CysHis: 0.188 ± 0.02
0.467CysIle: 0.467 ± 0.028
0.38CysLys: 0.38 ± 0.028
0.659CysLeu: 0.659 ± 0.035
0.164CysMet: 0.164 ± 0.018
0.309CysAsn: 0.309 ± 0.026
0.451CysPro: 0.451 ± 0.031
0.206CysGln: 0.206 ± 0.02
0.509CysArg: 0.509 ± 0.035
0.62CysSer: 0.62 ± 0.037
0.424CysThr: 0.424 ± 0.031
0.515CysVal: 0.515 ± 0.035
0.125CysTrp: 0.125 ± 0.016
0.249CysTyr: 0.249 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.021AspAla: 3.021 ± 0.071
0.542AspCys: 0.542 ± 0.033
2.666AspAsp: 2.666 ± 0.072
4.228AspGlu: 4.228 ± 0.101
3.153AspPhe: 3.153 ± 0.079
4.042AspGly: 4.042 ± 0.102
0.867AspHis: 0.867 ± 0.038
4.066AspIle: 4.066 ± 0.098
2.692AspLys: 2.692 ± 0.075
6.002AspLeu: 6.002 ± 0.131
1.229AspMet: 1.229 ± 0.055
1.932AspAsn: 1.932 ± 0.065
2.575AspPro: 2.575 ± 0.08
1.243AspGln: 1.243 ± 0.059
3.171AspArg: 3.171 ± 0.089
4.06AspSer: 4.06 ± 0.102
2.322AspThr: 2.322 ± 0.061
3.731AspVal: 3.731 ± 0.088
0.626AspTrp: 0.626 ± 0.04
2.395AspTyr: 2.395 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
5.281GluAla: 5.281 ± 0.103
0.424GluCys: 0.424 ± 0.028
3.89GluAsp: 3.89 ± 0.088
6.958GluGlu: 6.958 ± 0.15
3.27GluPhe: 3.27 ± 0.089
4.945GluGly: 4.945 ± 0.115
1.035GluHis: 1.035 ± 0.045
6.822GluIle: 6.822 ± 0.122
5.669GluLys: 5.669 ± 0.118
7.532GluLeu: 7.532 ± 0.143
2.201GluMet: 2.201 ± 0.06
3.256GluAsn: 3.256 ± 0.086
2.043GluPro: 2.043 ± 0.056
1.526GluGln: 1.526 ± 0.059
5.076GluArg: 5.076 ± 0.114
4.8GluSer: 4.8 ± 0.096
3.777GluThr: 3.777 ± 0.083
5.535GluVal: 5.535 ± 0.122
0.699GluTrp: 0.699 ± 0.039
2.554GluTyr: 2.554 ± 0.084
0.0GluXaa: 0.0 ± 0.0
Phe
3.286PheAla: 3.286 ± 0.089
0.467PheCys: 0.467 ± 0.035
3.064PheAsp: 3.064 ± 0.085
3.658PheGlu: 3.658 ± 0.095
2.635PhePhe: 2.635 ± 0.09
4.022PheGly: 4.022 ± 0.107
0.74PheHis: 0.74 ± 0.035
3.011PheIle: 3.011 ± 0.08
2.12PheLys: 2.12 ± 0.068
5.167PheLeu: 5.167 ± 0.128
1.227PheMet: 1.227 ± 0.052
1.687PheAsn: 1.687 ± 0.062
1.972PhePro: 1.972 ± 0.071
1.116PheGln: 1.116 ± 0.042
2.502PheArg: 2.502 ± 0.074
4.399PheSer: 4.399 ± 0.115
2.409PheThr: 2.409 ± 0.071
3.737PheVal: 3.737 ± 0.087
0.598PheTrp: 0.598 ± 0.034
1.558PheTyr: 1.558 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
4.571GlyAla: 4.571 ± 0.124
0.683GlyCys: 0.683 ± 0.039
3.702GlyAsp: 3.702 ± 0.091
5.184GlyGlu: 5.184 ± 0.131
3.979GlyPhe: 3.979 ± 0.096
5.056GlyGly: 5.056 ± 0.128
1.192GlyHis: 1.192 ± 0.052
6.269GlyIle: 6.269 ± 0.131
4.985GlyLys: 4.985 ± 0.121
6.738GlyLeu: 6.738 ± 0.136
2.098GlyMet: 2.098 ± 0.079
2.839GlyAsn: 2.839 ± 0.076
2.013GlyPro: 2.013 ± 0.07
1.637GlyGln: 1.637 ± 0.052
4.07GlyArg: 4.07 ± 0.098
5.125GlySer: 5.125 ± 0.116
3.973GlyThr: 3.973 ± 0.103
5.505GlyVal: 5.505 ± 0.118
0.879GlyTrp: 0.879 ± 0.045
2.902GlyTyr: 2.902 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.873HisAla: 0.873 ± 0.037
0.166HisCys: 0.166 ± 0.017
0.784HisAsp: 0.784 ± 0.043
1.043HisGlu: 1.043 ± 0.052
0.863HisPhe: 0.863 ± 0.043
1.211HisGly: 1.211 ± 0.047
0.309HisHis: 0.309 ± 0.026
1.136HisIle: 1.136 ± 0.052
0.645HisLys: 0.645 ± 0.036
1.491HisLeu: 1.491 ± 0.06
0.325HisMet: 0.325 ± 0.025
0.683HisAsn: 0.683 ± 0.037
0.776HisPro: 0.776 ± 0.043
0.321HisGln: 0.321 ± 0.026
0.845HisArg: 0.845 ± 0.045
1.126HisSer: 1.126 ± 0.047
0.711HisThr: 0.711 ± 0.041
0.978HisVal: 0.978 ± 0.045
0.164HisTrp: 0.164 ± 0.018
0.606HisTyr: 0.606 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.717IleAla: 5.717 ± 0.119
0.695IleCys: 0.695 ± 0.039
5.036IleAsp: 5.036 ± 0.119
6.253IleGlu: 6.253 ± 0.104
3.219IlePhe: 3.219 ± 0.089
5.434IleGly: 5.434 ± 0.126
1.128IleHis: 1.128 ± 0.052
4.743IleIle: 4.743 ± 0.102
3.365IleLys: 3.365 ± 0.09
7.063IleLeu: 7.063 ± 0.141
1.871IleMet: 1.871 ± 0.065
2.603IleAsn: 2.603 ± 0.069
3.476IlePro: 3.476 ± 0.085
1.631IleGln: 1.631 ± 0.061
3.813IleArg: 3.813 ± 0.087
5.852IleSer: 5.852 ± 0.125
3.848IleThr: 3.848 ± 0.092
6.289IleVal: 6.289 ± 0.116
0.711IleTrp: 0.711 ± 0.042
2.366IleTyr: 2.366 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
4.185LysAla: 4.185 ± 0.09
0.368LysCys: 0.368 ± 0.038
3.031LysAsp: 3.031 ± 0.094
4.988LysGlu: 4.988 ± 0.109
1.795LysPhe: 1.795 ± 0.066
4.068LysGly: 4.068 ± 0.102
0.857LysHis: 0.857 ± 0.034
4.391LysIle: 4.391 ± 0.091
4.167LysLys: 4.167 ± 0.122
5.171LysLeu: 5.171 ± 0.103
1.59LysMet: 1.59 ± 0.057
2.484LysAsn: 2.484 ± 0.079
2.051LysPro: 2.051 ± 0.061
1.215LysGln: 1.215 ± 0.052
3.678LysArg: 3.678 ± 0.083
4.106LysSer: 4.106 ± 0.092
3.332LysThr: 3.332 ± 0.081
3.904LysVal: 3.904 ± 0.103
0.54LysTrp: 0.54 ± 0.036
2.013LysTyr: 2.013 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
6.681LeuAla: 6.681 ± 0.134
0.764LeuCys: 0.764 ± 0.039
5.388LeuAsp: 5.388 ± 0.106
7.697LeuGlu: 7.697 ± 0.159
4.933LeuPhe: 4.933 ± 0.099
7.027LeuGly: 7.027 ± 0.134
1.429LeuHis: 1.429 ± 0.051
6.853LeuIle: 6.853 ± 0.141
6.713LeuLys: 6.713 ± 0.102
10.163LeuLeu: 10.163 ± 0.173
2.49LeuMet: 2.49 ± 0.076
3.763LeuAsn: 3.763 ± 0.085
3.991LeuPro: 3.991 ± 0.102
2.587LeuGln: 2.587 ± 0.074
5.555LeuArg: 5.555 ± 0.111
8.686LeuSer: 8.686 ± 0.141
4.886LeuThr: 4.886 ± 0.096
6.998LeuVal: 6.998 ± 0.135
0.855LeuTrp: 0.855 ± 0.043
2.855LeuTyr: 2.855 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
1.938MetAla: 1.938 ± 0.066
0.146MetCys: 0.146 ± 0.017
1.255MetAsp: 1.255 ± 0.054
1.79MetGlu: 1.79 ± 0.062
0.901MetPhe: 0.901 ± 0.045
1.803MetGly: 1.803 ± 0.063
0.317MetHis: 0.317 ± 0.026
2.181MetIle: 2.181 ± 0.07
2.411MetLys: 2.411 ± 0.063
2.29MetLeu: 2.29 ± 0.072
0.649MetMet: 0.649 ± 0.038
1.312MetAsn: 1.312 ± 0.048
0.893MetPro: 0.893 ± 0.044
0.511MetGln: 0.511 ± 0.032
1.584MetArg: 1.584 ± 0.054
1.696MetSer: 1.696 ± 0.06
1.354MetThr: 1.354 ± 0.051
1.712MetVal: 1.712 ± 0.064
0.182MetTrp: 0.182 ± 0.019
0.639MetTyr: 0.639 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
2.395AsnAla: 2.395 ± 0.076
0.386AsnCys: 0.386 ± 0.032
1.91AsnAsp: 1.91 ± 0.069
2.765AsnGlu: 2.765 ± 0.071
1.631AsnPhe: 1.631 ± 0.06
2.92AsnGly: 2.92 ± 0.09
0.655AsnHis: 0.655 ± 0.04
2.653AsnIle: 2.653 ± 0.077
1.811AsnLys: 1.811 ± 0.055
3.945AsnLeu: 3.945 ± 0.1
0.885AsnMet: 0.885 ± 0.043
1.316AsnAsn: 1.316 ± 0.054
2.015AsnPro: 2.015 ± 0.064
0.841AsnGln: 0.841 ± 0.045
2.14AsnArg: 2.14 ± 0.073
2.892AsnSer: 2.892 ± 0.083
1.772AsnThr: 1.772 ± 0.065
2.728AsnVal: 2.728 ± 0.073
0.479AsnTrp: 0.479 ± 0.031
1.512AsnTyr: 1.512 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
2.148ProAla: 2.148 ± 0.064
0.243ProCys: 0.243 ± 0.025
2.488ProAsp: 2.488 ± 0.066
3.508ProGlu: 3.508 ± 0.094
2.116ProPhe: 2.116 ± 0.064
2.837ProGly: 2.837 ± 0.075
0.546ProHis: 0.546 ± 0.032
2.457ProIle: 2.457 ± 0.072
1.661ProLys: 1.661 ± 0.058
3.476ProLeu: 3.476 ± 0.105
0.845ProMet: 0.845 ± 0.043
1.257ProAsn: 1.257 ± 0.049
1.18ProPro: 1.18 ± 0.051
0.942ProGln: 0.942 ± 0.041
1.455ProArg: 1.455 ± 0.053
2.704ProSer: 2.704 ± 0.066
1.896ProThr: 1.896 ± 0.058
3.367ProVal: 3.367 ± 0.081
0.469ProTrp: 0.469 ± 0.03
1.409ProTyr: 1.409 ± 0.058
0.0ProXaa: 0.0 ± 0.0
Gln
1.463GlnAla: 1.463 ± 0.054
0.162GlnCys: 0.162 ± 0.018
1.099GlnAsp: 1.099 ± 0.043
1.904GlnGlu: 1.904 ± 0.065
1.01GlnPhe: 1.01 ± 0.045
1.514GlnGly: 1.514 ± 0.058
0.303GlnHis: 0.303 ± 0.026
1.926GlnIle: 1.926 ± 0.069
1.455GlnLys: 1.455 ± 0.053
2.358GlnLeu: 2.358 ± 0.073
0.744GlnMet: 0.744 ± 0.041
1.035GlnAsn: 1.035 ± 0.046
0.705GlnPro: 0.705 ± 0.034
0.651GlnGln: 0.651 ± 0.034
1.279GlnArg: 1.279 ± 0.043
1.516GlnSer: 1.516 ± 0.054
1.128GlnThr: 1.128 ± 0.053
1.59GlnVal: 1.59 ± 0.055
0.261GlnTrp: 0.261 ± 0.023
0.746GlnTyr: 0.746 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
3.134ArgAla: 3.134 ± 0.077
0.416ArgCys: 0.416 ± 0.032
3.185ArgAsp: 3.185 ± 0.069
5.008ArgGlu: 5.008 ± 0.12
2.952ArgPhe: 2.952 ± 0.08
3.359ArgGly: 3.359 ± 0.089
0.812ArgHis: 0.812 ± 0.042
4.729ArgIle: 4.729 ± 0.102
3.676ArgLys: 3.676 ± 0.087
5.751ArgLeu: 5.751 ± 0.119
1.52ArgMet: 1.52 ± 0.051
2.267ArgAsn: 2.267 ± 0.07
1.675ArgPro: 1.675 ± 0.061
1.293ArgGln: 1.293 ± 0.049
3.268ArgArg: 3.268 ± 0.101
3.605ArgSer: 3.605 ± 0.098
2.496ArgThr: 2.496 ± 0.071
4.088ArgVal: 4.088 ± 0.101
0.548ArgTrp: 0.548 ± 0.032
2.029ArgTyr: 2.029 ± 0.066
0.0ArgXaa: 0.0 ± 0.0
Ser
4.452SerAla: 4.452 ± 0.106
0.651SerCys: 0.651 ± 0.041
3.775SerAsp: 3.775 ± 0.1
5.42SerGlu: 5.42 ± 0.09
4.147SerPhe: 4.147 ± 0.079
6.244SerGly: 6.244 ± 0.109
1.107SerHis: 1.107 ± 0.046
5.568SerIle: 5.568 ± 0.113
3.997SerLys: 3.997 ± 0.098
8.086SerLeu: 8.086 ± 0.152
1.916SerMet: 1.916 ± 0.066
2.575SerAsn: 2.575 ± 0.078
2.744SerPro: 2.744 ± 0.073
1.687SerGln: 1.687 ± 0.065
4.325SerArg: 4.325 ± 0.109
5.964SerSer: 5.964 ± 0.118
3.468SerThr: 3.468 ± 0.097
5.565SerVal: 5.565 ± 0.097
0.784SerTrp: 0.784 ± 0.043
2.445SerTyr: 2.445 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
3.528ThrAla: 3.528 ± 0.094
0.291ThrCys: 0.291 ± 0.024
2.601ThrAsp: 2.601 ± 0.086
3.264ThrGlu: 3.264 ± 0.077
2.379ThrPhe: 2.379 ± 0.073
4.387ThrGly: 4.387 ± 0.097
0.732ThrHis: 0.732 ± 0.04
3.775ThrIle: 3.775 ± 0.111
2.263ThrLys: 2.263 ± 0.068
5.167ThrLeu: 5.167 ± 0.11
1.273ThrMet: 1.273 ± 0.049
1.696ThrAsn: 1.696 ± 0.064
2.193ThrPro: 2.193 ± 0.069
0.968ThrGln: 0.968 ± 0.044
2.346ThrArg: 2.346 ± 0.067
3.355ThrSer: 3.355 ± 0.084
2.49ThrThr: 2.49 ± 0.077
4.29ThrVal: 4.29 ± 0.122
0.443ThrTrp: 0.443 ± 0.035
1.588ThrTyr: 1.588 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
5.167ValAla: 5.167 ± 0.113
0.62ValCys: 0.62 ± 0.035
4.484ValAsp: 4.484 ± 0.106
5.662ValGlu: 5.662 ± 0.115
4.171ValPhe: 4.171 ± 0.095
5.056ValGly: 5.056 ± 0.102
1.093ValHis: 1.093 ± 0.051
5.65ValIle: 5.65 ± 0.113
4.052ValLys: 4.052 ± 0.1
7.344ValLeu: 7.344 ± 0.133
1.734ValMet: 1.734 ± 0.063
2.69ValAsn: 2.69 ± 0.074
2.866ValPro: 2.866 ± 0.075
1.855ValGln: 1.855 ± 0.056
3.876ValArg: 3.876 ± 0.105
6.046ValSer: 6.046 ± 0.117
3.779ValThr: 3.779 ± 0.087
6.206ValVal: 6.206 ± 0.126
0.663ValTrp: 0.663 ± 0.041
2.405ValTyr: 2.405 ± 0.076
0.0ValXaa: 0.0 ± 0.0
Trp
0.59TrpAla: 0.59 ± 0.036
0.067TrpCys: 0.067 ± 0.012
0.531TrpAsp: 0.531 ± 0.037
0.699TrpGlu: 0.699 ± 0.04
0.467TrpPhe: 0.467 ± 0.027
0.732TrpGly: 0.732 ± 0.038
0.158TrpHis: 0.158 ± 0.017
0.768TrpIle: 0.768 ± 0.039
0.8TrpLys: 0.8 ± 0.046
1.039TrpLeu: 1.039 ± 0.048
0.299TrpMet: 0.299 ± 0.028
0.489TrpAsn: 0.489 ± 0.035
0.335TrpPro: 0.335 ± 0.027
0.364TrpGln: 0.364 ± 0.03
0.473TrpArg: 0.473 ± 0.032
0.719TrpSer: 0.719 ± 0.043
0.493TrpThr: 0.493 ± 0.031
0.608TrpVal: 0.608 ± 0.036
0.182TrpTrp: 0.182 ± 0.025
0.382TrpTyr: 0.382 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.116TyrAla: 2.116 ± 0.063
0.368TyrCys: 0.368 ± 0.029
2.051TyrAsp: 2.051 ± 0.07
2.352TyrGlu: 2.352 ± 0.07
1.942TyrPhe: 1.942 ± 0.075
2.684TyrGly: 2.684 ± 0.074
0.594TyrHis: 0.594 ± 0.037
2.164TyrIle: 2.164 ± 0.066
1.431TyrLys: 1.431 ± 0.059
3.712TyrLeu: 3.712 ± 0.091
0.723TyrMet: 0.723 ± 0.042
1.164TyrAsn: 1.164 ± 0.055
1.303TyrPro: 1.303 ± 0.052
0.829TyrGln: 0.829 ± 0.037
2.156TyrArg: 2.156 ± 0.077
2.91TyrSer: 2.91 ± 0.075
1.441TyrThr: 1.441 ± 0.057
2.36TyrVal: 2.36 ± 0.078
0.42TyrTrp: 0.42 ± 0.03
1.461TyrTyr: 1.461 ± 0.066
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1662 proteins (494837 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski