Mariniradius saccharolyticus AK6
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4633 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M7X9M5|M7X9M5_9BACT Rhomboid family protein OS=Mariniradius saccharolyticus AK6 OX=1239962 GN=C943_01910 PE=4 SV=1
MM1 pKa = 7.44 NPTIVFGQGFNNNEE15 pKa = 4.02 WVFGDD20 pKa = 4.91 CGTGQNNYY28 pKa = 10.36 LSFGKK33 pKa = 10.65 GGDD36 pKa = 3.82 PIVRR40 pKa = 11.84 TLPSGVIVGQNNNAVAIDD58 pKa = 4.74 PISGNIIFYY67 pKa = 10.7 SNGVLVYY74 pKa = 10.37 DD75 pKa = 4.71 ANNQILEE82 pKa = 4.27 GVAPGINGDD91 pKa = 3.56 INGQQQVGIGILSYY105 pKa = 10.54 EE106 pKa = 4.13 PQGDD110 pKa = 3.11 RR111 pKa = 11.84 LYY113 pKa = 11.06 YY114 pKa = 9.84 IFYY117 pKa = 10.34 VSPAGDD123 pKa = 3.62 LQYY126 pKa = 12.09 ALIDD130 pKa = 3.72 MNAPGQAVGNEE141 pKa = 4.05 PPLGEE146 pKa = 5.15 IIEE149 pKa = 4.36 KK150 pKa = 10.49 DD151 pKa = 3.5 QVVVGAVNGPILVVKK166 pKa = 9.09 TPQSPSYY173 pKa = 10.1 LISIEE178 pKa = 3.99 GGSIVSRR185 pKa = 11.84 RR186 pKa = 11.84 IGANPGEE193 pKa = 4.22 FTLISNAAVPFTPKK207 pKa = 10.37 FMVFDD212 pKa = 3.87 EE213 pKa = 4.49 ASQRR217 pKa = 11.84 VILIPDD223 pKa = 4.32 DD224 pKa = 4.12 PNQNIVVLGFDD235 pKa = 3.65 TASGTFGSQEE245 pKa = 4.47 PISQSSNGQAYY256 pKa = 9.81 GGASFSPDD264 pKa = 2.54 GAFIYY269 pKa = 9.73 FSRR272 pKa = 11.84 GNQLFRR278 pKa = 11.84 IPSNNLTASPEE289 pKa = 4.41 AIPLDD294 pKa = 4.03 NPVHH298 pKa = 6.93 AIYY301 pKa = 10.18 DD302 pKa = 3.91 VKK304 pKa = 11.03 VGPDD308 pKa = 2.85 GRR310 pKa = 11.84 LYY312 pKa = 10.67 YY313 pKa = 9.94 IYY315 pKa = 10.49 EE316 pKa = 4.15 EE317 pKa = 4.33 VLGGPQLIGRR327 pKa = 11.84 VNNPNEE333 pKa = 4.12 PVLDD337 pKa = 4.17 DD338 pKa = 4.63 LVLAEE343 pKa = 5.25 DD344 pKa = 4.7 PFNGTDD350 pKa = 3.47 FCGRR354 pKa = 11.84 IFPKK358 pKa = 10.41 FAPNATISPTVDD370 pKa = 4.01 FTWEE374 pKa = 3.73 PDD376 pKa = 3.63 QPCSNNPVQLTSLITPEE393 pKa = 3.69 NYY395 pKa = 9.83 RR396 pKa = 11.84 PVSFEE401 pKa = 3.59 WTFNPPLVDD410 pKa = 3.73 EE411 pKa = 5.6 DD412 pKa = 4.28 GQPVDD417 pKa = 3.34 IDD419 pKa = 3.57 FTQEE423 pKa = 3.47 HH424 pKa = 6.7 LLIPEE429 pKa = 4.84 DD430 pKa = 3.34 ATANQSIDD438 pKa = 3.25 VTLTVTFADD447 pKa = 4.79 GSTQTVNKK455 pKa = 9.24 TISLLEE461 pKa = 4.16 NDD463 pKa = 4.39 LQVQFSSQDD472 pKa = 3.23 TTVCEE477 pKa = 4.55 GACVDD482 pKa = 3.62 IGSMLEE488 pKa = 3.96 VQQGGGQGGGQGGGQGNYY506 pKa = 9.45 EE507 pKa = 4.24 YY508 pKa = 10.0 FWSNIRR514 pKa = 11.84 EE515 pKa = 4.13 WRR517 pKa = 11.84 SSKK520 pKa = 10.77 DD521 pKa = 3.24 NCVDD525 pKa = 3.75 LPGLYY530 pKa = 8.72 WVLVRR535 pKa = 11.84 EE536 pKa = 4.76 PGSSCYY542 pKa = 10.37 AYY544 pKa = 9.8 GSVRR548 pKa = 11.84 VRR550 pKa = 11.84 IWDD553 pKa = 4.85 LLDD556 pKa = 3.11 QSNNVWYY563 pKa = 10.11 FGDD566 pKa = 4.18 GAGLDD571 pKa = 4.3 FNPDD575 pKa = 3.63 PNNPNAPLPRR585 pKa = 11.84 PVGHH589 pKa = 6.26 NQNIPAGTTTISDD602 pKa = 3.35 GTGQVLFFTDD612 pKa = 5.3 GEE614 pKa = 4.97 TVWDD618 pKa = 3.94 LNGNIMEE625 pKa = 4.92 NGDD628 pKa = 4.23 SIGGSNLSSQSVIAVPVPTDD648 pKa = 3.07 EE649 pKa = 4.55 TIFYY653 pKa = 10.89 LFTTQLAQDD662 pKa = 4.33 GSNQVKK668 pKa = 9.9 FSVVDD673 pKa = 3.98 IKK675 pKa = 11.45 AEE677 pKa = 3.97 NQNGVGNVVSKK688 pKa = 11.44 DD689 pKa = 3.27 NFLFSPSTEE698 pKa = 3.76 HH699 pKa = 6.8 SAALAAGDD707 pKa = 3.95 TTWVLFHH714 pKa = 7.06 EE715 pKa = 5.11 LGNNTFRR722 pKa = 11.84 AYY724 pKa = 9.44 PVSIHH729 pKa = 7.26 GIGQPVLSSVGSSHH743 pKa = 6.38 GFNSGVGSMKK753 pKa = 10.43 FSPDD757 pKa = 2.97 GTKK760 pKa = 10.44 VAVTISDD767 pKa = 4.35 GACSRR772 pKa = 11.84 LEE774 pKa = 4.37 IFDD777 pKa = 4.46 FDD779 pKa = 4.15 QNTGEE784 pKa = 4.09 MTEE787 pKa = 4.12 YY788 pKa = 11.42 ALLDD792 pKa = 3.97 LGCNNDD798 pKa = 3.11 QVYY801 pKa = 10.72 GLEE804 pKa = 3.99 FSNDD808 pKa = 2.81 GSRR811 pKa = 11.84 VYY813 pKa = 10.81 VSYY816 pKa = 10.92 RR817 pKa = 11.84 GGNGKK822 pKa = 8.93 IDD824 pKa = 3.76 EE825 pKa = 4.75 FLIQAPEE832 pKa = 3.86 QTGNNPIPCATCFSSATTRR851 pKa = 11.84 PQRR854 pKa = 11.84 EE855 pKa = 3.86 ACILNSPVRR864 pKa = 11.84 NTLTTSGPFGALQIGPDD881 pKa = 3.55 GQIYY885 pKa = 8.59 VARR888 pKa = 11.84 PGQNVLGTINAGQDD902 pKa = 4.0 CNRR905 pKa = 11.84 SAYY908 pKa = 10.04 NEE910 pKa = 4.32 NGTSTLLGTSNLGLPSFVQQSGSSIPEE937 pKa = 3.75 PALAGTPFLCIDD949 pKa = 4.06 PQNGALGEE957 pKa = 4.19 FEE959 pKa = 5.44 GGGEE963 pKa = 4.17 PDD965 pKa = 2.93 IDD967 pKa = 3.99 SYY969 pKa = 11.62 FWTIVHH975 pKa = 6.9 EE976 pKa = 5.02 DD977 pKa = 3.29 GTSILNNYY985 pKa = 8.14 GGPGEE990 pKa = 4.03 NFQVLEE996 pKa = 4.2 QPFTRR1001 pKa = 11.84 PGTYY1005 pKa = 8.87 TVSLRR1010 pKa = 11.84 VDD1012 pKa = 3.06 RR1013 pKa = 11.84 CGDD1016 pKa = 3.37 PDD1018 pKa = 4.01 FYY1020 pKa = 11.05 EE1021 pKa = 4.66 ASLEE1025 pKa = 4.27 VEE1027 pKa = 4.88 VIAPPTLTLPGEE1039 pKa = 4.3 ATLCSGNPVSLTAIDD1054 pKa = 5.26 GYY1056 pKa = 11.51 DD1057 pKa = 3.76 PADD1060 pKa = 3.52 GLYY1063 pKa = 10.83 DD1064 pKa = 3.84 FEE1066 pKa = 4.45 WRR1068 pKa = 11.84 NAQGTLFGDD1077 pKa = 4.11 SNSNTIFVNEE1087 pKa = 3.94 EE1088 pKa = 4.07 SIFTVTVSHH1097 pKa = 7.0 RR1098 pKa = 11.84 RR1099 pKa = 11.84 PDD1101 pKa = 4.52 DD1102 pKa = 3.37 IPAEE1106 pKa = 4.11 FFNPCPATASVFVGPPFDD1124 pKa = 5.08 FDD1126 pKa = 3.71 LTQTADD1132 pKa = 3.26 QVCFDD1137 pKa = 3.76 EE1138 pKa = 4.72 SLVVFAPNTPIVGEE1152 pKa = 3.87 WFYY1155 pKa = 11.38 QVQGTNNRR1163 pKa = 11.84 VSLGDD1168 pKa = 3.62 FFEE1171 pKa = 6.18 LEE1173 pKa = 4.26 LVANTLPGPGIYY1185 pKa = 10.03 DD1186 pKa = 3.82 IIFVAEE1192 pKa = 4.2 DD1193 pKa = 4.54 PIVPGCLVEE1202 pKa = 5.13 KK1203 pKa = 10.42 VVEE1206 pKa = 4.62 LIVWPLPAVEE1216 pKa = 5.04 AVVLTDD1222 pKa = 5.46 ADD1224 pKa = 3.67 ACGQPTGSFEE1234 pKa = 4.02 ITALTAADD1242 pKa = 4.33 EE1243 pKa = 4.78 IIVVEE1248 pKa = 4.25 TGQRR1252 pKa = 11.84 FTNVNAGDD1260 pKa = 3.74 VLPIVSNLEE1269 pKa = 3.84 PGVYY1273 pKa = 8.31 TVRR1276 pKa = 11.84 ATNDD1280 pKa = 3.37 TGCEE1284 pKa = 3.64 FSLPVQIRR1292 pKa = 11.84 NLNPPTGIEE1301 pKa = 4.03 DD1302 pKa = 4.95 FIVQASPEE1310 pKa = 4.1 VCGPVGVEE1318 pKa = 3.65 NGEE1321 pKa = 4.2 LLVVFPAGPQTGTYY1335 pKa = 9.64 RR1336 pKa = 11.84 AIRR1339 pKa = 11.84 VEE1341 pKa = 4.16 NGLEE1345 pKa = 3.96 TTGAFTNEE1353 pKa = 3.57 DD1354 pKa = 3.38 SVRR1357 pKa = 11.84 ISVEE1361 pKa = 3.3 AGTYY1365 pKa = 8.51 TIEE1368 pKa = 6.29 IIDD1371 pKa = 3.84 QFNCSVPDD1379 pKa = 4.07 PGQYY1383 pKa = 9.86 IVDD1386 pKa = 3.56 EE1387 pKa = 4.47 RR1388 pKa = 11.84 EE1389 pKa = 3.65 QAEE1392 pKa = 3.99 FSIPNDD1398 pKa = 3.35 LSICQEE1404 pKa = 4.02 FTFTPVSQQNLVYY1417 pKa = 10.43 TLVGPNNQPITPNQDD1432 pKa = 2.24 GSYY1435 pKa = 8.62 TISVTGQYY1443 pKa = 10.5 TMTGTDD1449 pKa = 4.13 PNGLLCPAQRR1459 pKa = 11.84 QITILNNIFVDD1470 pKa = 4.61 FAVSFAQVDD1479 pKa = 4.13 CALGQYY1485 pKa = 10.27 FDD1487 pKa = 6.09 AILNNTNPGNVNFFWRR1503 pKa = 11.84 NEE1505 pKa = 3.76 AGDD1508 pKa = 3.54 IVGRR1512 pKa = 11.84 DD1513 pKa = 3.29 QRR1515 pKa = 11.84 FFPPAPGTYY1524 pKa = 9.85 SLEE1527 pKa = 3.98 VQPRR1531 pKa = 11.84 SGALCPPRR1539 pKa = 11.84 IITFTAPPYY1548 pKa = 10.49 ALSVDD1553 pKa = 3.56 AALEE1557 pKa = 4.16 VIPFCVEE1564 pKa = 3.6 DD1565 pKa = 3.79 AFTVVSLEE1573 pKa = 3.75 ADD1575 pKa = 3.22 QTFVEE1580 pKa = 4.9 RR1581 pKa = 11.84 IDD1583 pKa = 3.34 WLFVVNNNPVALPQYY1598 pKa = 10.8 EE1599 pKa = 4.39 NLTSINVSDD1608 pKa = 3.55 EE1609 pKa = 4.3 GIYY1612 pKa = 10.1 QVVLTSVDD1620 pKa = 3.17 GCEE1623 pKa = 4.07 LARR1626 pKa = 11.84 EE1627 pKa = 4.14 QIQVIRR1633 pKa = 11.84 STIVPPVIDD1642 pKa = 3.91 PVYY1645 pKa = 10.11 IICAVEE1651 pKa = 4.42 GVTATITPGTYY1662 pKa = 8.42 STYY1665 pKa = 9.71 RR1666 pKa = 11.84 WLLNGVEE1673 pKa = 4.3 ISTAPEE1679 pKa = 3.92 LTPTEE1684 pKa = 4.26 AGSYY1688 pKa = 10.21 LLTVGDD1694 pKa = 5.43 DD1695 pKa = 3.99 LGCSYY1700 pKa = 10.55 TVSFTVNEE1708 pKa = 4.1 DD1709 pKa = 3.21 CALRR1713 pKa = 11.84 VIFPDD1718 pKa = 4.16 AMTPSNPSKK1727 pKa = 10.55 RR1728 pKa = 11.84 FEE1730 pKa = 4.28 VYY1732 pKa = 10.48 TNDD1735 pKa = 3.23 FVSDD1739 pKa = 3.84 LEE1741 pKa = 4.26 VFIFNRR1747 pKa = 11.84 WGEE1750 pKa = 4.29 LIFYY1754 pKa = 7.64 CQQSNINGEE1763 pKa = 4.15 INVCQWDD1770 pKa = 3.94 GTVDD1774 pKa = 3.69 GKK1776 pKa = 10.43 YY1777 pKa = 10.29 VPIGVYY1783 pKa = 9.75 PVVVKK1788 pKa = 10.57 YY1789 pKa = 10.63 RR1790 pKa = 11.84 SQRR1793 pKa = 11.84 QNVTNSITKK1802 pKa = 10.0 SIIVIEE1808 pKa = 4.16
Molecular weight: 195.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.884
IPC_protein 3.91
Toseland 3.694
ProMoST 4.037
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.795
Sillero 4.024
Patrickios 0.642
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|M7XA83|M7XA83_9BACT GCN5-related N-acetyltransferase OS=Mariniradius saccharolyticus AK6 OX=1239962 GN=C943_03544 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.5 HH16 pKa = 3.99 GFRR19 pKa = 11.84 EE20 pKa = 4.18 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 RR29 pKa = 11.84 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 HH40 pKa = 5.24 KK41 pKa = 10.87 LSVSSEE47 pKa = 3.97 KK48 pKa = 9.91 TLKK51 pKa = 10.51 KK52 pKa = 10.72
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.368
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.281
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.003
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.088
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4633
0
4633
1435942
37
4749
309.9
34.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.076 ± 0.035
0.679 ± 0.011
5.3 ± 0.026
6.607 ± 0.034
5.514 ± 0.031
7.302 ± 0.034
1.896 ± 0.02
6.928 ± 0.028
6.587 ± 0.038
9.722 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.508 ± 0.018
4.811 ± 0.028
4.173 ± 0.022
3.735 ± 0.018
4.436 ± 0.024
6.265 ± 0.026
5.042 ± 0.031
6.478 ± 0.031
1.347 ± 0.013
3.592 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here