Nonomuraea pusilla

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8486 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8K2W3|A0A1H8K2W3_9ACTN Uncharacterized protein OS=Nonomuraea pusilla OX=46177 GN=SAMN05660976_08508 PE=4 SV=1
MM1 pKa = 8.08AYY3 pKa = 9.5PSSGWDD9 pKa = 3.31GKK11 pKa = 11.23FMTVAAADD19 pKa = 3.65VRR21 pKa = 11.84PTDD24 pKa = 3.57VVLNPARR31 pKa = 11.84YY32 pKa = 7.22VTRR35 pKa = 11.84TADD38 pKa = 3.95LPDD41 pKa = 3.48GMIDD45 pKa = 3.42IFRR48 pKa = 11.84PAHH51 pKa = 6.19PSQPCEE57 pKa = 4.04GCDD60 pKa = 3.49ARR62 pKa = 11.84PGEE65 pKa = 4.28PCEE68 pKa = 4.06WDD70 pKa = 3.95CLGLAALDD78 pKa = 4.24DD79 pKa = 3.72ATGYY83 pKa = 11.07GFADD87 pKa = 3.57VGG89 pKa = 3.7

Molecular weight: 9.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7XVC4|A0A1H7XVC4_9ACTN Uncharacterized protein OS=Nonomuraea pusilla OX=46177 GN=SAMN05660976_04984 PE=4 SV=1
MM1 pKa = 7.42FLRR4 pKa = 11.84VALALQTVALLVQAVTAGMLLSSPGGRR31 pKa = 11.84AAHH34 pKa = 6.84AATAIAVVVTVLVHH48 pKa = 6.27LVAAVMTRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84AVIMPAVTMLLMTVVQIALAVAHH81 pKa = 6.6LKK83 pKa = 9.96VLHH86 pKa = 6.11VPVGVLMFGVSLMRR100 pKa = 11.84VSALWPSRR108 pKa = 11.84RR109 pKa = 11.84AAAVPAA115 pKa = 4.36

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8486

0

8486

2733672

31

3143

322.1

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.867 ± 0.041

0.785 ± 0.008

5.651 ± 0.021

5.625 ± 0.029

2.764 ± 0.014

9.637 ± 0.028

2.194 ± 0.012

3.083 ± 0.019

1.877 ± 0.021

10.776 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.01

1.576 ± 0.015

6.301 ± 0.028

2.569 ± 0.017

8.513 ± 0.027

4.815 ± 0.016

5.6 ± 0.024

8.894 ± 0.029

1.569 ± 0.011

2.071 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski