Human herpesvirus 2 (HHV-2) (Human herpes simplex virus 2)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9I272|G9I272_HHV2 Envelope glycoprotein N OS=Human herpesvirus 2 OX=10310 GN=UL49A PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 6.11LLVDD6 pKa = 5.22DD7 pKa = 5.74LFADD11 pKa = 4.15ADD13 pKa = 4.36GVSPPPPRR21 pKa = 11.84PAGGPKK27 pKa = 8.47NTPAAPPLYY36 pKa = 9.66ATGRR40 pKa = 11.84LSQAQLMPSPPMPVPPAALFNRR62 pKa = 11.84LLDD65 pKa = 4.24DD66 pKa = 4.82LGFSAGPALCTMLDD80 pKa = 3.31TWNEE84 pKa = 3.9DD85 pKa = 3.45LFSGFPTNADD95 pKa = 3.44MYY97 pKa = 10.28RR98 pKa = 11.84EE99 pKa = 4.25CKK101 pKa = 9.87FLSTLPSDD109 pKa = 3.98VIDD112 pKa = 3.62WGDD115 pKa = 2.96AHH117 pKa = 7.92VPEE120 pKa = 5.48RR121 pKa = 11.84SPIDD125 pKa = 3.23IRR127 pKa = 11.84AHH129 pKa = 5.98GDD131 pKa = 3.29VAFPTLPATRR141 pKa = 11.84DD142 pKa = 3.71EE143 pKa = 4.42LPSYY147 pKa = 9.84YY148 pKa = 10.37EE149 pKa = 3.91AMAQFFRR156 pKa = 11.84GEE158 pKa = 3.49LRR160 pKa = 11.84ARR162 pKa = 11.84EE163 pKa = 3.85EE164 pKa = 4.07SYY166 pKa = 9.67RR167 pKa = 11.84TVLANFCSALYY178 pKa = 9.82RR179 pKa = 11.84YY180 pKa = 9.56LRR182 pKa = 11.84ASVRR186 pKa = 11.84QLHH189 pKa = 5.87RR190 pKa = 11.84QAHH193 pKa = 4.42MRR195 pKa = 11.84GRR197 pKa = 11.84NRR199 pKa = 11.84DD200 pKa = 3.5LRR202 pKa = 11.84EE203 pKa = 3.77MLRR206 pKa = 11.84TTIADD211 pKa = 3.77RR212 pKa = 11.84YY213 pKa = 8.15YY214 pKa = 11.05RR215 pKa = 11.84EE216 pKa = 3.99TARR219 pKa = 11.84LARR222 pKa = 11.84VLFLHH227 pKa = 7.12LYY229 pKa = 10.01LFLSRR234 pKa = 11.84EE235 pKa = 4.35ILWAAYY241 pKa = 9.3AEE243 pKa = 4.24QMMRR247 pKa = 11.84PDD249 pKa = 5.09LFDD252 pKa = 4.58GLCCDD257 pKa = 4.14LEE259 pKa = 4.27SWRR262 pKa = 11.84QLACLFQPLMFINGSLTVRR281 pKa = 11.84GVPVEE286 pKa = 3.73ARR288 pKa = 11.84RR289 pKa = 11.84LRR291 pKa = 11.84EE292 pKa = 3.74LNHH295 pKa = 6.26IRR297 pKa = 11.84EE298 pKa = 4.48HH299 pKa = 6.62LNLPLVRR306 pKa = 11.84SAAAEE311 pKa = 4.11EE312 pKa = 4.45PGAPLTTPPVLQGNQARR329 pKa = 11.84SSGYY333 pKa = 10.32FMLLIRR339 pKa = 11.84AKK341 pKa = 10.0LDD343 pKa = 3.46SYY345 pKa = 11.74SSVATSEE352 pKa = 4.56GEE354 pKa = 4.24SVMRR358 pKa = 11.84EE359 pKa = 3.61HH360 pKa = 7.3AYY362 pKa = 10.74SRR364 pKa = 11.84GRR366 pKa = 11.84TRR368 pKa = 11.84NNYY371 pKa = 9.34GSTIEE376 pKa = 4.57GLLDD380 pKa = 5.1LPDD383 pKa = 5.66DD384 pKa = 5.23DD385 pKa = 5.87DD386 pKa = 5.94APAEE390 pKa = 4.39AGLVAPRR397 pKa = 11.84MSFLSAGQRR406 pKa = 11.84PRR408 pKa = 11.84RR409 pKa = 11.84LSTTAPITDD418 pKa = 3.2VSLGDD423 pKa = 3.68EE424 pKa = 4.19LRR426 pKa = 11.84LDD428 pKa = 3.93GEE430 pKa = 4.64EE431 pKa = 4.29VDD433 pKa = 4.2MTPADD438 pKa = 3.84ALDD441 pKa = 4.69DD442 pKa = 4.45FDD444 pKa = 6.91LEE446 pKa = 4.23MLGDD450 pKa = 3.97VEE452 pKa = 4.78SPSPGMTHH460 pKa = 7.45DD461 pKa = 3.87PVSYY465 pKa = 10.51GALDD469 pKa = 3.39VDD471 pKa = 4.18DD472 pKa = 6.2FEE474 pKa = 4.83FEE476 pKa = 4.75QMFTDD481 pKa = 5.11AMGIDD486 pKa = 4.2DD487 pKa = 4.9FGGG490 pKa = 3.31

Molecular weight: 54.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6PUY5|D6PUY5_HHV2 Capsid vertex component 2 OS=Human herpesvirus 2 OX=10310 GN=UL25 PE=1 SV=1
MM1 pKa = 7.08TSRR4 pKa = 11.84RR5 pKa = 11.84SVKK8 pKa = 9.82SCPRR12 pKa = 11.84EE13 pKa = 4.0APRR16 pKa = 11.84GTHH19 pKa = 5.71EE20 pKa = 4.05EE21 pKa = 4.49LYY23 pKa = 10.27YY24 pKa = 11.29GPVSPADD31 pKa = 3.61PEE33 pKa = 4.33SPRR36 pKa = 11.84DD37 pKa = 3.64DD38 pKa = 3.91FRR40 pKa = 11.84RR41 pKa = 11.84GAGPMRR47 pKa = 11.84ARR49 pKa = 11.84PRR51 pKa = 11.84GEE53 pKa = 3.77VRR55 pKa = 11.84FLHH58 pKa = 6.22YY59 pKa = 10.97DD60 pKa = 3.31EE61 pKa = 6.13AGYY64 pKa = 11.17ALYY67 pKa = 10.14RR68 pKa = 11.84DD69 pKa = 4.14SSSSEE74 pKa = 4.22DD75 pKa = 3.38NDD77 pKa = 3.34EE78 pKa = 4.45SRR80 pKa = 11.84DD81 pKa = 3.44TARR84 pKa = 11.84PRR86 pKa = 11.84RR87 pKa = 11.84SASVAGSHH95 pKa = 6.7GPGPARR101 pKa = 11.84APPPPGGPVGAGGRR115 pKa = 11.84SHH117 pKa = 7.29APPARR122 pKa = 11.84TPKK125 pKa = 7.61MTRR128 pKa = 11.84GAPKK132 pKa = 10.48APATPATDD140 pKa = 3.65PARR143 pKa = 11.84GRR145 pKa = 11.84RR146 pKa = 11.84PAQADD151 pKa = 3.66SAVLLDD157 pKa = 4.59APAPTASGRR166 pKa = 11.84TKK168 pKa = 10.47TPAQGLAKK176 pKa = 10.08KK177 pKa = 9.8LHH179 pKa = 6.61FSTAPPSPTAPWTPRR194 pKa = 11.84VAGFNKK200 pKa = 9.92RR201 pKa = 11.84VFCAAVGRR209 pKa = 11.84LAATHH214 pKa = 6.23ARR216 pKa = 11.84LAAVQLWDD224 pKa = 3.32MSRR227 pKa = 11.84PHH229 pKa = 6.58TDD231 pKa = 2.86EE232 pKa = 5.39DD233 pKa = 4.48LNEE236 pKa = 4.43LLDD239 pKa = 3.76LTTIRR244 pKa = 11.84VTVCEE249 pKa = 4.57GKK251 pKa = 10.09NLLQRR256 pKa = 11.84ANEE259 pKa = 4.16LVNPDD264 pKa = 3.15AAQDD268 pKa = 3.57VDD270 pKa = 3.4ATAAARR276 pKa = 11.84GRR278 pKa = 11.84PAGRR282 pKa = 11.84AAATARR288 pKa = 11.84APARR292 pKa = 11.84SASRR296 pKa = 11.84PRR298 pKa = 11.84RR299 pKa = 11.84PLEE302 pKa = 3.76

Molecular weight: 32.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

39131

86

3119

528.8

56.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.677 ± 0.495

1.791 ± 0.114

5.246 ± 0.141

4.907 ± 0.149

3.378 ± 0.18

7.792 ± 0.231

2.591 ± 0.095

2.745 ± 0.146

1.607 ± 0.148

9.652 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.651 ± 0.101

2.078 ± 0.149

9.026 ± 0.432

2.865 ± 0.129

8.709 ± 0.193

6.074 ± 0.228

5.597 ± 0.205

6.943 ± 0.175

1.163 ± 0.063

2.507 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski