Streptococcus phage Javan139

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A0M4|A0A4D6A0M4_9CAUD Portal protein OS=Streptococcus phage Javan139 OX=2548000 GN=Javan139_0024 PE=4 SV=1
MM1 pKa = 7.49INWNDD6 pKa = 2.94VSLNDD11 pKa = 3.7VICYY15 pKa = 8.94YY16 pKa = 11.36AFVFDD21 pKa = 5.47LDD23 pKa = 3.33ISLVLEE29 pKa = 4.67FSVHH33 pKa = 6.73DD34 pKa = 4.22LDD36 pKa = 6.18EE37 pKa = 4.47IALCKK42 pKa = 10.18LAIEE46 pKa = 4.13NWKK49 pKa = 10.54KK50 pKa = 11.08SEE52 pKa = 3.86

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A1X8|A0A4D6A1X8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan139 OX=2548000 GN=Javan139_0005 PE=4 SV=1
MM1 pKa = 7.57WIEE4 pKa = 3.88EE5 pKa = 3.99QSNGKK10 pKa = 8.81LRR12 pKa = 11.84YY13 pKa = 7.6VEE15 pKa = 4.67RR16 pKa = 11.84YY17 pKa = 8.82KK18 pKa = 10.81EE19 pKa = 4.19HH20 pKa = 5.75YY21 pKa = 7.4TGKK24 pKa = 10.35YY25 pKa = 7.74KK26 pKa = 10.52RR27 pKa = 11.84AYY29 pKa = 9.59EE30 pKa = 4.42LIEE33 pKa = 4.69RR34 pKa = 11.84DD35 pKa = 3.54TPQGRR40 pKa = 11.84KK41 pKa = 8.92KK42 pKa = 10.56AATALRR48 pKa = 11.84LKK50 pKa = 10.23IEE52 pKa = 4.24KK53 pKa = 9.76LVHH56 pKa = 5.68IRR58 pKa = 11.84PKK60 pKa = 10.25KK61 pKa = 8.81EE62 pKa = 3.4KK63 pKa = 8.23VTFGEE68 pKa = 5.03VYY70 pKa = 10.57QNFYY74 pKa = 11.15SSWSLGVKK82 pKa = 10.01QSTIYY87 pKa = 8.8STKK90 pKa = 10.54NIDD93 pKa = 3.91RR94 pKa = 11.84IILKK98 pKa = 9.95KK99 pKa = 10.38IPNDD103 pKa = 3.46YY104 pKa = 10.59LISKK108 pKa = 9.34IDD110 pKa = 3.32RR111 pKa = 11.84RR112 pKa = 11.84FLQKK116 pKa = 10.37IFDD119 pKa = 4.51DD120 pKa = 4.34LLSEE124 pKa = 4.5GRR126 pKa = 11.84SHH128 pKa = 7.43NYY130 pKa = 8.04VKK132 pKa = 10.48KK133 pKa = 11.1VKK135 pKa = 9.22WKK137 pKa = 9.75LNRR140 pKa = 11.84IFKK143 pKa = 10.15YY144 pKa = 10.08ALRR147 pKa = 11.84MDD149 pKa = 4.56YY150 pKa = 10.79IDD152 pKa = 4.11VNEE155 pKa = 4.64MIHH158 pKa = 6.88VEE160 pKa = 4.12LPKK163 pKa = 10.6EE164 pKa = 3.79ILTAEE169 pKa = 3.93QAKK172 pKa = 10.02KK173 pKa = 10.46KK174 pKa = 10.26KK175 pKa = 10.32EE176 pKa = 3.78KK177 pKa = 10.62FLDD180 pKa = 3.41QKK182 pKa = 10.12EE183 pKa = 4.5FKK185 pKa = 10.84LFAQNLRR192 pKa = 11.84EE193 pKa = 4.1EE194 pKa = 4.51VYY196 pKa = 10.48RR197 pKa = 11.84DD198 pKa = 3.62YY199 pKa = 11.02RR200 pKa = 11.84VKK202 pKa = 10.54KK203 pKa = 8.25YY204 pKa = 10.97LRR206 pKa = 11.84IAIVLYY212 pKa = 8.7LTGMRR217 pKa = 11.84YY218 pKa = 10.29GEE220 pKa = 3.98LAGLNISKK228 pKa = 10.82DD229 pKa = 2.81IDD231 pKa = 3.95FKK233 pKa = 11.44KK234 pKa = 9.62KK235 pKa = 8.9TIHH238 pKa = 6.07IQHH241 pKa = 6.61TFNFRR246 pKa = 11.84TKK248 pKa = 10.47QRR250 pKa = 11.84TTPKK254 pKa = 9.63TKK256 pKa = 10.15GSDD259 pKa = 3.57RR260 pKa = 11.84IIEE263 pKa = 4.14VSDD266 pKa = 3.6TVLKK270 pKa = 10.81NIQEE274 pKa = 4.05QLAEE278 pKa = 4.24NIRR281 pKa = 11.84AGFEE285 pKa = 3.67TDD287 pKa = 3.43YY288 pKa = 11.7LFINTLGYY296 pKa = 6.77PTTPEE301 pKa = 3.68RR302 pKa = 11.84VIGAFKK308 pKa = 10.74RR309 pKa = 11.84HH310 pKa = 4.87GQKK313 pKa = 10.66AGIEE317 pKa = 4.3KK318 pKa = 10.73NITTHH323 pKa = 6.57IFRR326 pKa = 11.84HH327 pKa = 4.67SHH329 pKa = 5.38ISLLAEE335 pKa = 3.99IGIPLPAIMEE345 pKa = 4.41RR346 pKa = 11.84VGHH349 pKa = 6.2SDD351 pKa = 2.74SKK353 pKa = 8.85TTLEE357 pKa = 4.47VYY359 pKa = 10.4SHH361 pKa = 6.15VTLQMTTNLAKK372 pKa = 10.66KK373 pKa = 9.96LNEE376 pKa = 3.98VKK378 pKa = 10.78LFF380 pKa = 3.85

Molecular weight: 44.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11044

44

1133

204.5

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.891 ± 0.455

0.561 ± 0.11

6.655 ± 0.407

7.687 ± 0.314

3.903 ± 0.248

5.994 ± 0.362

1.512 ± 0.156

7.905 ± 0.286

8.756 ± 0.387

8.511 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.155

5.714 ± 0.263

2.608 ± 0.221

3.866 ± 0.212

4.138 ± 0.297

6.112 ± 0.335

5.804 ± 0.333

5.931 ± 0.28

1.177 ± 0.143

3.866 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski