Pseudomonas phage POR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9SG24|A0A0N9SG24_9CAUD Putative pyrophosphatase OS=Pseudomonas phage POR1 OX=1718594 GN=POR1_29 PE=4 SV=1
MM1 pKa = 6.61QTIEE5 pKa = 5.21LYY7 pKa = 10.87SDD9 pKa = 3.33DD10 pKa = 4.88DD11 pKa = 4.99YY12 pKa = 12.04YY13 pKa = 11.89VQLLGDD19 pKa = 3.64GVLFYY24 pKa = 11.37LHH26 pKa = 6.09MQWNSEE32 pKa = 4.13AEE34 pKa = 4.18FWTLAIEE41 pKa = 4.43NYY43 pKa = 9.73NRR45 pKa = 11.84EE46 pKa = 4.18LLVSGVKK53 pKa = 9.54VVPNIRR59 pKa = 11.84LLKK62 pKa = 9.73LYY64 pKa = 10.08RR65 pKa = 11.84YY66 pKa = 9.21IDD68 pKa = 3.7GMPTGEE74 pKa = 5.53LIALSDD80 pKa = 3.62SQAISKK86 pKa = 9.36TDD88 pKa = 3.59FAEE91 pKa = 5.93GYY93 pKa = 9.68AQMVYY98 pKa = 7.82VTAPEE103 pKa = 4.3LPQQ106 pKa = 4.93

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9SH86|A0A0N9SH86_9CAUD Uncharacterized protein OS=Pseudomonas phage POR1 OX=1718594 GN=POR1_66 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.31KK3 pKa = 10.43FYY5 pKa = 9.97WRR7 pKa = 11.84LCFIWHH13 pKa = 6.48ICRR16 pKa = 11.84FLSFEE21 pKa = 4.2RR22 pKa = 11.84HH23 pKa = 4.39WLRR26 pKa = 11.84LGWRR30 pKa = 11.84ASKK33 pKa = 10.18RR34 pKa = 11.84WYY36 pKa = 10.41GYY38 pKa = 10.47EE39 pKa = 4.13SLTAKK44 pKa = 9.13QAAMRR49 pKa = 11.84EE50 pKa = 3.79IDD52 pKa = 3.32GWHH55 pKa = 4.86VV56 pKa = 2.92

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

17296

39

873

213.5

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.893 ± 0.359

1.064 ± 0.146

5.602 ± 0.199

6.036 ± 0.262

3.602 ± 0.168

7.915 ± 0.35

2.105 ± 0.231

4.539 ± 0.2

5.053 ± 0.247

7.828 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.15

4.515 ± 0.177

4.724 ± 0.32

4.463 ± 0.474

6.134 ± 0.29

5.741 ± 0.2

5.932 ± 0.302

6.666 ± 0.243

1.393 ± 0.124

3.145 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski